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The map label for this gene is curA [H]

Identifier: 158314223

GI number: 158314223

Start: 2847022

End: 2848374

Strand: Direct

Name: curA [H]

Synonym: Franean1_2393

Alternate gene names: 158314223

Gene position: 2847022-2848374 (Clockwise)

Preceding gene: 158314222

Following gene: 158314224

Centisome position: 31.7

GC content: 73.1

Gene sequence:

>1353_bases
GTGAGCAAACCCACGCAGCGGTCGCGGCCGGACGACGCTCACGGGCCGGCGTCACGGGACGGGGGTCCGCGCCGCACCGC
GGTGACCGGGGTCGGCGTCGTCGCCCCCGGTGGCTCGAACCGCAAAGAGTTCTGGACCCTGCTCACAGACGGCCGCACCG
CCACCCGCCGGCTGTCCTTCTTCGACCCGTCGCCGTTCCGGTCGCAGGTCGCGGCCGAGTGCGACTTCGACCCGCTCGCC
GCCGGCCTCACCCGCCAGGAGATCGCGCGCAACGACCGGTTCGTGCAGTTCGCGCTGGCCGCGGCCGCGGAGGCGGTGGC
CGACAGCGGGTTGGACCTCGGCCTCGGTGTCGGCGGCACCGAACGCGAGCCGGGCGACGGGCCGGTCGTCGGGGTGAGCA
TGGGCAGCGCCGTCGGGGCGACCACCCGGCTCGAGAACGAGTACGTGGTCGTCAGCGACGGCGGGAAGCACTGGGAGGTC
GACCCGCGCTACGCGAGCTCGTTCCTCTACCACGCGCTCGTGCCGAGCTGCCTGGCCACCGAGATCGCCTGCCGCTTCGG
CGCGCACGGCCCGTCGTTCGTGGTCTCCACCGGGTGCACCTCCGGCATCGACGCGGTCGGCTACGGCCACCAGCTCATCG
TCGACGGCGAGGCGGACATCGTGATCGCCGGCGCCTCCGACGCGCCGCTCTCGCCGATCTCCATCGCCTGCTTCGACGCC
ATCCGCGCCACCTCGGCCCGCAACGACGACCCCGAGCACGCCTCGCGGCCGTTCGACGCCAGCCGCGACGGCTTCGTGAT
GGGGGAGGGCAGCGCCGTGCTGATCCTCGAGGAGCTGGAGTCGGCCCGGGCCCGCGGCGCGCACATCTACTGCGAGGTCG
GCGGGTACGCCTCGCGGTCCAACGCTTTCCACATGACCGGCCTGCGCCCGGACGGCGTCGAGATGGCCGAGGCCATCCGC
ATCGCCCTGGACCAGGCGCGGCTCGACCCGTCCGCCGTCGACTACGTCAACGCCCACGGGTCGGGGACGAAGCAGAACGA
CCGGCACGAGACGGCCGCTTTCAAACGGTCGCTGGGCGCCCACGCCCACGAGGTGCCGGTCAGCTCGATCAAGTCGATGG
TCGGCCACTCCCTCGGCGCGATCGGCTCCATCGAGCTCGCGGCCTGCGCGCTGGCGATCGAGCACAACGTGGTGCCGCCG
ACGGCGAACTGGTCGACCCGCGACCCCGAGTGCGACCTGGACTACGTCCCGAACACCGCCCGCGACCACACGGTGGACGC
GGCGCTGTCGGTCGGCAGCGGGTTCGGCGGGTTCCAGTCGGCCATCGTCCTGACCTCGGCGGACCGGGCATGA

Upstream 100 bases:

>100_bases
GCGACGACGTGGCCTTCCTCGTCTTCCACCTCAGCCCGCTCGCGCCGCGGCCCGACCTGGGGCACGTGGACACCGAGCCG
CTGCCTGAGCAGTCGCAGCA

Downstream 100 bases:

>100_bases
CCGTCCTCGACACCGCGCCGTCCGGGACGGCCGGCCCGGCCCGGCCGGCCGCGGGCGCGGTGCGGCCGGTCATCACCGGG
CTCGGGGTCATCGCGCCCAG

Product: beta-ketoacyl synthase

Products: [acyl carrier protein]; acetoacetyl-ACP; CO2; [acyl carrier protein]; beta-ketoacyl-ACP [C]

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MSKPTQRSRPDDAHGPASRDGGPRRTAVTGVGVVAPGGSNRKEFWTLLTDGRTATRRLSFFDPSPFRSQVAAECDFDPLA
AGLTRQEIARNDRFVQFALAAAAEAVADSGLDLGLGVGGTEREPGDGPVVGVSMGSAVGATTRLENEYVVVSDGGKHWEV
DPRYASSFLYHALVPSCLATEIACRFGAHGPSFVVSTGCTSGIDAVGYGHQLIVDGEADIVIAGASDAPLSPISIACFDA
IRATSARNDDPEHASRPFDASRDGFVMGEGSAVLILEELESARARGAHIYCEVGGYASRSNAFHMTGLRPDGVEMAEAIR
IALDQARLDPSAVDYVNAHGSGTKQNDRHETAAFKRSLGAHAHEVPVSSIKSMVGHSLGAIGSIELAACALAIEHNVVPP
TANWSTRDPECDLDYVPNTARDHTVDAALSVGSGFGGFQSAIVLTSADRA

Sequences:

>Translated_450_residues
MSKPTQRSRPDDAHGPASRDGGPRRTAVTGVGVVAPGGSNRKEFWTLLTDGRTATRRLSFFDPSPFRSQVAAECDFDPLA
AGLTRQEIARNDRFVQFALAAAAEAVADSGLDLGLGVGGTEREPGDGPVVGVSMGSAVGATTRLENEYVVVSDGGKHWEV
DPRYASSFLYHALVPSCLATEIACRFGAHGPSFVVSTGCTSGIDAVGYGHQLIVDGEADIVIAGASDAPLSPISIACFDA
IRATSARNDDPEHASRPFDASRDGFVMGEGSAVLILEELESARARGAHIYCEVGGYASRSNAFHMTGLRPDGVEMAEAIR
IALDQARLDPSAVDYVNAHGSGTKQNDRHETAAFKRSLGAHAHEVPVSSIKSMVGHSLGAIGSIELAACALAIEHNVVPP
TANWSTRDPECDLDYVPNTARDHTVDAALSVGSGFGGFQSAIVLTSADRA
>Mature_449_residues
SKPTQRSRPDDAHGPASRDGGPRRTAVTGVGVVAPGGSNRKEFWTLLTDGRTATRRLSFFDPSPFRSQVAAECDFDPLAA
GLTRQEIARNDRFVQFALAAAAEAVADSGLDLGLGVGGTEREPGDGPVVGVSMGSAVGATTRLENEYVVVSDGGKHWEVD
PRYASSFLYHALVPSCLATEIACRFGAHGPSFVVSTGCTSGIDAVGYGHQLIVDGEADIVIAGASDAPLSPISIACFDAI
RATSARNDDPEHASRPFDASRDGFVMGEGSAVLILEELESARARGAHIYCEVGGYASRSNAFHMTGLRPDGVEMAEAIRI
ALDQARLDPSAVDYVNAHGSGTKQNDRHETAAFKRSLGAHAHEVPVSSIKSMVGHSLGAIGSIELAACALAIEHNVVPPT
ANWSTRDPECDLDYVPNTARDHTVDAALSVGSGFGGFQSAIVLTSADRA

Specific function: Catalyzes The Condensation Reaction Of Fatty Acid Synthesis By The Addition To An Acyl Acceptor Of Two Carbons From Malonyl-Acp. Has A Preference For Short Chain Acid Substrates And May Function To Supply The Octanoic Substrates For Lipoic Acid Biosynthe

COG id: COG0304

COG function: function code IQ; 3-oxoacyl-(acyl-carrier-protein) synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-ketoacyl-ACP synthases family [H]

Homologues:

Organism=Homo sapiens, GI8923559, Length=432, Percent_Identity=34.0277777777778, Blast_Score=210, Evalue=3e-54,
Organism=Homo sapiens, GI41872631, Length=437, Percent_Identity=24.9427917620137, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI223972627, Length=169, Percent_Identity=32.5443786982249, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI1787337, Length=422, Percent_Identity=35.781990521327, Blast_Score=247, Evalue=1e-66,
Organism=Escherichia coli, GI1788663, Length=426, Percent_Identity=31.2206572769953, Blast_Score=168, Evalue=5e-43,
Organism=Caenorhabditis elegans, GI71983541, Length=426, Percent_Identity=29.3427230046948, Blast_Score=151, Evalue=7e-37,
Organism=Caenorhabditis elegans, GI71983535, Length=254, Percent_Identity=35.0393700787402, Blast_Score=141, Evalue=8e-34,
Organism=Caenorhabditis elegans, GI212642053, Length=441, Percent_Identity=24.9433106575964, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17550940, Length=225, Percent_Identity=27.1111111111111, Blast_Score=72, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6320904, Length=465, Percent_Identity=29.4623655913978, Blast_Score=164, Evalue=3e-41,
Organism=Drosophila melanogaster, GI24644341, Length=437, Percent_Identity=38.6727688787185, Blast_Score=223, Evalue=2e-58,
Organism=Drosophila melanogaster, GI19920632, Length=473, Percent_Identity=24.3128964059197, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI221330659, Length=473, Percent_Identity=24.3128964059197, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24581345, Length=304, Percent_Identity=25.6578947368421, Blast_Score=90, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000794
- InterPro:   IPR018201
- InterPro:   IPR014031
- InterPro:   IPR014030
- InterPro:   IPR016039
- InterPro:   IPR016038 [H]

Pfam domain/function: PF00109 ketoacyl-synt; PF02801 Ketoacyl-synt_C [H]

EC number: 2.3.1.41 [C]

Molecular weight: Translated: 47056; Mature: 46924

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00606 B_KETOACYL_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKPTQRSRPDDAHGPASRDGGPRRTAVTGVGVVAPGGSNRKEFWTLLTDGRTATRRLSF
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCEEEEEEECCCCHHEEEEC
FDPSPFRSQVAAECDFDPLAAGLTRQEIARNDRFVQFALAAAAEAVADSGLDLGLGVGGT
CCCCHHHHHHHHCCCCCHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEECCCCC
EREPGDGPVVGVSMGSAVGATTRLENEYVVVSDGGKHWEVDPRYASSFLYHALVPSCLAT
CCCCCCCCEEEEECCCCCCCEEEECCCEEEEECCCCEEECCHHHHHHHHHHHHHHHHHHH
EIACRFGAHGPSFVVSTGCTSGIDAVGYGHQLIVDGEADIVIAGASDAPLSPISIACFDA
HHHHHHCCCCCCEEEECCCCCCCHHCCCCCEEEECCCCCEEEECCCCCCCCCHHHHHHHH
IRATSARNDDPEHASRPFDASRDGFVMGEGSAVLILEELESARARGAHIYCEVGGYASRS
HHHHCCCCCCHHHHCCCCCCCCCCEEEECCCEEEEEHHHHHHHCCCCEEEEEECCCCCCC
NAFHMTGLRPDGVEMAEAIRIALDQARLDPSAVDYVNAHGSGTKQNDRHETAAFKRSLGA
CCEEECCCCCCCHHHHHHHHHHHHHHCCCCCHHHEEECCCCCCCCCCHHHHHHHHHHHCC
HAHEVPVSSIKSMVGHSLGAIGSIELAACALAIEHNVVPPTANWSTRDPECDLDYVPNTA
CHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
RDHTVDAALSVGSGFGGFQSAIVLTSADRA
CCCHHHHHHCCCCCCCCCCEEEEEEECCCC
>Mature Secondary Structure 
SKPTQRSRPDDAHGPASRDGGPRRTAVTGVGVVAPGGSNRKEFWTLLTDGRTATRRLSF
CCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCEEEEEEECCCCHHEEEEC
FDPSPFRSQVAAECDFDPLAAGLTRQEIARNDRFVQFALAAAAEAVADSGLDLGLGVGGT
CCCCHHHHHHHHCCCCCHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEECCCCC
EREPGDGPVVGVSMGSAVGATTRLENEYVVVSDGGKHWEVDPRYASSFLYHALVPSCLAT
CCCCCCCCEEEEECCCCCCCEEEECCCEEEEECCCCEEECCHHHHHHHHHHHHHHHHHHH
EIACRFGAHGPSFVVSTGCTSGIDAVGYGHQLIVDGEADIVIAGASDAPLSPISIACFDA
HHHHHHCCCCCCEEEECCCCCCCHHCCCCCEEEECCCCCEEEECCCCCCCCCHHHHHHHH
IRATSARNDDPEHASRPFDASRDGFVMGEGSAVLILEELESARARGAHIYCEVGGYASRS
HHHHCCCCCCHHHHCCCCCCCCCCEEEECCCEEEEEHHHHHHHCCCCEEEEEECCCCCCC
NAFHMTGLRPDGVEMAEAIRIALDQARLDPSAVDYVNAHGSGTKQNDRHETAAFKRSLGA
CCEEECCCCCCCHHHHHHHHHHHHHHCCCCCHHHEEECCCCCCCCCCHHHHHHHHHHHCC
HAHEVPVSSIKSMVGHSLGAIGSIELAACALAIEHNVVPPTANWSTRDPECDLDYVPNTA
CHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
RDHTVDAALSVGSGFGGFQSAIVLTSADRA
CCCHHHHHHCCCCCCCCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: None [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.138 {cis-9-hexadecenoyl-ACP}} 0.017 {cis-9-hexadecenoyl-ACP}} 0.012 {cis-3-decenoyl-ACP}} 0.04 {acetyl-ACP}} 0.031 {acetyl-ACP}} [C]

Substrates: acetyl-ACP; malonyl-ACP; acyl-ACP [C]

Specific reaction: acetyl-ACP + malonyl-ACP = [acyl carrier protein] + acetoacetyl-ACP + CO2 acyl-ACP + malonyl-ACP = [acyl carrier protein] + beta-ketoacyl-ACP + CO2 [C]

General reaction: Acyl group transfer [C]

Inhibitor: Acetyl-CoA; Acyl-CoA; Iodoacetamide; N-Ethyl maleimide acyl -ACP-derivatives protect, malonyl-ACP-decarboxylation, Cerulenin [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1644304 [H]