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The map label for this gene is lepA [H]

Identifier: 158313940

GI number: 158313940

Start: 2531627

End: 2533528

Strand: Direct

Name: lepA [H]

Synonym: Franean1_2106

Alternate gene names: 158313940

Gene position: 2531627-2533528 (Clockwise)

Preceding gene: 158313938

Following gene: 158313942

Centisome position: 28.19

GC content: 68.03

Gene sequence:

>1902_bases
GTGCCCGACAGACCGACGAGCAGCGAAACGAGACCAGCCCGCGTGTCCGAAGCCCCGCACGAGGCATCCGGCGTCGACCC
CGCGTCGATCCGTAACTTCTGCATCATCGCCCACATCGACCACGGCAAGTCCACGCTCGCCGACCGCATGCTGGGCGTGA
CCGGTGTGGTCGAGGCACGCAACATGCGCGCGCAGTACCTCGACCGGATGGACATCGAGCGCGAGCGCGGCATCACGATC
AAGGCGCAGAACGTCCGGCTGCCCTGGCGCGCCGACGACGGCCAGGACTACGTCCTGCACCTCATCGACACGCCCGGCCA
CGTGGACTTCACCTACGAGGTGAGCCGCTCGCTCGCGGCCTGTGAGGGCGCGGTGCTGCTGGTCGACGCGGCCCAGGGCA
TCGAGGCGCAGACACTCGCCAACCTGTACCTGGCGATCGAGAACGACCTGGCGATCATCCCGGTGCTCAACAAGATCGAC
CTGCCGGCCGCGCAGCCGGAGAAGTGCGCCGCGGAGATCTCCTCAATCATCGGCTGCGACCCGGGCGACGTCCTCAAGGT
CTCCGGCAAGACCGGCCAGGGCGTGCCGGAGCTGCTCAACGAGATCGTCCGCCGTATCCCGGCGCCGAGCGGCGACCCGG
ACGGCCCGCTCCGTGCCATGATCTTCGACAGCGTGTACGACATCTACCGCGGTGTGATCACCTATGTCCGGGTGGTGGAC
GGGTCGCTCAGCACCCGTGACCGTTGCCTGATGATGTCCACCGGGACCAGCCACGAGACCCTCGAGGTCGGTGTCATCTC
GCCGGAGGCCCAACCGACCGGGTCGCTGTCGGCAGGTGAGGTCGGCTATGTCATCCCCGGTGTGAAGGACGTCCGCCAGG
CCCGTGTCGGGGACACGATCACCTCGGCGCGCCGCCCGGCCACGGAGATGCTCGGCGGCTACCGGGATCCGCGTCCGATG
GTCTACTCGGGGCTGTATCCCCTGGACGGCAGCGAGTACCCGGCGCTGCGCGAGGCGCTGGACAAGCTGCAGCTCAACGA
CGCGGCGCTCACCTTCGAGCCGGAGACGTCGGCGGCGCTGGGGTTCGGCTTCCGGTGTGGCTTCCTCGGGCTGCTCCATC
TGGAGATCGTCCGGGAGCGGCTGGAGCGGGAGTTCGACCTCACGCTCATCTCGACGGCCCCCAACGTGGTCTACCGGGTC
GTCATGGAGGACACGTCCGAGGTCACGGTGACCAACCCCAGCGACTGGCCCGGCGGCAAGATCGCCGAGGTGTACGAGCC
GGTCGTGGAGGCGATGCTGCTGCTGCCCACCGACTTCGTCGGCGCCGTGATGGAGCTGTGTCAGGGCCGGCGCGGCGTGC
TCAAGGGGATGGACTACATCTCCACCGACCGGGTGGAACTGAAGTACACGCTGCCGCTCGGTGAGATCATCTTCGACTTC
TTCGACGCGCTGAAGTCGCGCACCCGCGGCTACGCCAGCCTCGACTACGAGCCCGCCGGCGAGCAGGCCTCGGACCTGGT
CAAGGTGGACATCCTCCTGCAGGGCGAGGCGGTGGACGCGTTCTCGGCGATCGTGCACCGGGAGAAGGCCTACTCCTACG
GAGTGGCGATGACCTCGAAGCTGCGGGAGCTGATTCCCCGCCAGCAGTTCGAGGTGCCGATCCAGGCGGCCATCGGCAGC
CGGATCATCGCCCGGGAGAACATCCGCGCCATCCGCAAGGACGTCCTCGCCAAGTGCTACGGCGGTGACATCAGCCGTAA
GCGGAAGCTGCTGGAGAAGCAGAAGGAGGGCAAGAAGCGGATGAAGACCATCGGCCGGGTGGAGGTCCCGCAGGAGGCGT
TCATCGCGGCACTGTCCACGACCGAGAGCCCGAAGGCGCCGGCGCCCAAGACCGGGCGGTAG

Upstream 100 bases:

>100_bases
CGGGCGGGCGTGGCGCGCCTGGCACGATGGGGGCCTGGCACGATGAAGGACTCGAGCCCGTGCACATGACAGTCCGTGCA
CACGGCGCCAGCCGGACCGA

Downstream 100 bases:

>100_bases
CGGCGCGGCGGTCCGCACCATGCGAGCGGCACGGCGGCCCGGCCGGCCCTTCGCGGGCCGGGCGCGGGCGGGCGGCCGTC
AGACGTTCTCGGGAGCCGCG

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA [H]

Number of amino acids: Translated: 633; Mature: 632

Protein sequence:

>633_residues
MPDRPTSSETRPARVSEAPHEASGVDPASIRNFCIIAHIDHGKSTLADRMLGVTGVVEARNMRAQYLDRMDIERERGITI
KAQNVRLPWRADDGQDYVLHLIDTPGHVDFTYEVSRSLAACEGAVLLVDAAQGIEAQTLANLYLAIENDLAIIPVLNKID
LPAAQPEKCAAEISSIIGCDPGDVLKVSGKTGQGVPELLNEIVRRIPAPSGDPDGPLRAMIFDSVYDIYRGVITYVRVVD
GSLSTRDRCLMMSTGTSHETLEVGVISPEAQPTGSLSAGEVGYVIPGVKDVRQARVGDTITSARRPATEMLGGYRDPRPM
VYSGLYPLDGSEYPALREALDKLQLNDAALTFEPETSAALGFGFRCGFLGLLHLEIVRERLEREFDLTLISTAPNVVYRV
VMEDTSEVTVTNPSDWPGGKIAEVYEPVVEAMLLLPTDFVGAVMELCQGRRGVLKGMDYISTDRVELKYTLPLGEIIFDF
FDALKSRTRGYASLDYEPAGEQASDLVKVDILLQGEAVDAFSAIVHREKAYSYGVAMTSKLRELIPRQQFEVPIQAAIGS
RIIARENIRAIRKDVLAKCYGGDISRKRKLLEKQKEGKKRMKTIGRVEVPQEAFIAALSTTESPKAPAPKTGR

Sequences:

>Translated_633_residues
MPDRPTSSETRPARVSEAPHEASGVDPASIRNFCIIAHIDHGKSTLADRMLGVTGVVEARNMRAQYLDRMDIERERGITI
KAQNVRLPWRADDGQDYVLHLIDTPGHVDFTYEVSRSLAACEGAVLLVDAAQGIEAQTLANLYLAIENDLAIIPVLNKID
LPAAQPEKCAAEISSIIGCDPGDVLKVSGKTGQGVPELLNEIVRRIPAPSGDPDGPLRAMIFDSVYDIYRGVITYVRVVD
GSLSTRDRCLMMSTGTSHETLEVGVISPEAQPTGSLSAGEVGYVIPGVKDVRQARVGDTITSARRPATEMLGGYRDPRPM
VYSGLYPLDGSEYPALREALDKLQLNDAALTFEPETSAALGFGFRCGFLGLLHLEIVRERLEREFDLTLISTAPNVVYRV
VMEDTSEVTVTNPSDWPGGKIAEVYEPVVEAMLLLPTDFVGAVMELCQGRRGVLKGMDYISTDRVELKYTLPLGEIIFDF
FDALKSRTRGYASLDYEPAGEQASDLVKVDILLQGEAVDAFSAIVHREKAYSYGVAMTSKLRELIPRQQFEVPIQAAIGS
RIIARENIRAIRKDVLAKCYGGDISRKRKLLEKQKEGKKRMKTIGRVEVPQEAFIAALSTTESPKAPAPKTGR
>Mature_632_residues
PDRPTSSETRPARVSEAPHEASGVDPASIRNFCIIAHIDHGKSTLADRMLGVTGVVEARNMRAQYLDRMDIERERGITIK
AQNVRLPWRADDGQDYVLHLIDTPGHVDFTYEVSRSLAACEGAVLLVDAAQGIEAQTLANLYLAIENDLAIIPVLNKIDL
PAAQPEKCAAEISSIIGCDPGDVLKVSGKTGQGVPELLNEIVRRIPAPSGDPDGPLRAMIFDSVYDIYRGVITYVRVVDG
SLSTRDRCLMMSTGTSHETLEVGVISPEAQPTGSLSAGEVGYVIPGVKDVRQARVGDTITSARRPATEMLGGYRDPRPMV
YSGLYPLDGSEYPALREALDKLQLNDAALTFEPETSAALGFGFRCGFLGLLHLEIVRERLEREFDLTLISTAPNVVYRVV
MEDTSEVTVTNPSDWPGGKIAEVYEPVVEAMLLLPTDFVGAVMELCQGRRGVLKGMDYISTDRVELKYTLPLGEIIFDFF
DALKSRTRGYASLDYEPAGEQASDLVKVDILLQGEAVDAFSAIVHREKAYSYGVAMTSKLRELIPRQQFEVPIQAAIGSR
IIARENIRAIRKDVLAKCYGGDISRKRKLLEKQKEGKKRMKTIGRVEVPQEAFIAALSTTESPKAPAPKTGR

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily [H]

Homologues:

Organism=Homo sapiens, GI157426893, Length=604, Percent_Identity=49.8344370860927, Blast_Score=634, Evalue=0.0,
Organism=Homo sapiens, GI94966754, Length=136, Percent_Identity=41.1764705882353, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI18390331, Length=161, Percent_Identity=36.6459627329193, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI25306287, Length=136, Percent_Identity=44.8529411764706, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI25306283, Length=136, Percent_Identity=44.8529411764706, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI19923640, Length=136, Percent_Identity=44.8529411764706, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI4503483, Length=145, Percent_Identity=37.2413793103448, Blast_Score=100, Evalue=6e-21,
Organism=Homo sapiens, GI310132016, Length=113, Percent_Identity=38.0530973451327, Blast_Score=96, Evalue=8e-20,
Organism=Homo sapiens, GI310110807, Length=113, Percent_Identity=38.0530973451327, Blast_Score=96, Evalue=8e-20,
Organism=Homo sapiens, GI310123363, Length=113, Percent_Identity=38.0530973451327, Blast_Score=96, Evalue=8e-20,
Organism=Homo sapiens, GI217272894, Length=156, Percent_Identity=34.6153846153846, Blast_Score=87, Evalue=5e-17,
Organism=Homo sapiens, GI217272892, Length=156, Percent_Identity=34.6153846153846, Blast_Score=87, Evalue=5e-17,
Organism=Homo sapiens, GI53729339, Length=216, Percent_Identity=28.7037037037037, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI53729337, Length=216, Percent_Identity=28.7037037037037, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1788922, Length=598, Percent_Identity=55.5183946488294, Blast_Score=650, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=475, Percent_Identity=28.8421052631579, Blast_Score=151, Evalue=1e-37,
Organism=Escherichia coli, GI1789738, Length=159, Percent_Identity=36.4779874213836, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1790835, Length=184, Percent_Identity=31.5217391304348, Blast_Score=89, Evalue=9e-19,
Organism=Escherichia coli, GI1789559, Length=248, Percent_Identity=26.2096774193548, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17557151, Length=616, Percent_Identity=40.4220779220779, Blast_Score=470, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI71988811, Length=145, Percent_Identity=39.3103448275862, Blast_Score=105, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI71988819, Length=145, Percent_Identity=39.3103448275862, Blast_Score=105, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17556745, Length=158, Percent_Identity=34.1772151898734, Blast_Score=97, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17533571, Length=164, Percent_Identity=39.0243902439024, Blast_Score=97, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17506493, Length=219, Percent_Identity=29.6803652968037, Blast_Score=92, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17552882, Length=152, Percent_Identity=34.2105263157895, Blast_Score=91, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6323320, Length=622, Percent_Identity=45.3376205787781, Blast_Score=555, Evalue=1e-158,
Organism=Saccharomyces cerevisiae, GI6323098, Length=200, Percent_Identity=33, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6324707, Length=144, Percent_Identity=36.8055555555556, Blast_Score=100, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6320593, Length=144, Percent_Identity=36.8055555555556, Blast_Score=100, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6322359, Length=114, Percent_Identity=42.1052631578947, Blast_Score=98, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6324166, Length=157, Percent_Identity=38.8535031847134, Blast_Score=97, Evalue=5e-21,
Organism=Drosophila melanogaster, GI78706572, Length=598, Percent_Identity=45.819397993311, Blast_Score=539, Evalue=1e-153,
Organism=Drosophila melanogaster, GI28574573, Length=136, Percent_Identity=42.6470588235294, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24582462, Length=152, Percent_Identity=37.5, Blast_Score=101, Evalue=2e-21,
Organism=Drosophila melanogaster, GI221458488, Length=160, Percent_Identity=39.375, Blast_Score=98, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24585709, Length=148, Percent_Identity=34.4594594594595, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24585711, Length=148, Percent_Identity=34.4594594594595, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24585713, Length=148, Percent_Identity=34.4594594594595, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21357743, Length=138, Percent_Identity=36.9565217391304, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI28572034, Length=247, Percent_Identity=28.7449392712551, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI45550900, Length=352, Percent_Identity=26.1363636363636, Blast_Score=70, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C [H]

EC number: NA

Molecular weight: Translated: 69319; Mature: 69188

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDRPTSSETRPARVSEAPHEASGVDPASIRNFCIIAHIDHGKSTLADRMLGVTGVVEAR
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCEEEEEEECCCHHHHHHHHHHHHHHHHHH
NMRAQYLDRMDIERERGITIKAQNVRLPWRADDGQDYVLHLIDTPGHVDFTYEVSRSLAA
HHHHHHHHHHCCHHHCCCEEEECCEECCEECCCCCCEEEEEECCCCCEEEEHHHHHHHHH
CEGAVLLVDAAQGIEAQTLANLYLAIENDLAIIPVLNKIDLPAAQPEKCAAEISSIIGCD
HCCCEEEEECCCCCCHHHHHHEEEEEECCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCC
PGDVLKVSGKTGQGVPELLNEIVRRIPAPSGDPDGPLRAMIFDSVYDIYRGVITYVRVVD
CCCEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
GSLSTRDRCLMMSTGTSHETLEVGVISPEAQPTGSLSAGEVGYVIPGVKDVRQARVGDTI
CCCCCCCCEEEEECCCCCCEEEEEEECCCCCCCCCCCCCCCCEECCCHHHHHHHHCCCHH
TSARRPATEMLGGYRDPRPMVYSGLYPLDGSEYPALREALDKLQLNDAALTFEPETSAAL
HHHCCCHHHHHCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCHH
GFGFRCGFLGLLHLEIVRERLEREFDLTLISTAPNVVYRVVMEDTSEVTVTNPSDWPGGK
CCCHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCEEEEEECCCCEEEEECCCCCCCCC
IAEVYEPVVEAMLLLPTDFVGAVMELCQGRRGVLKGMDYISTDRVELKYTLPLGEIIFDF
HHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHCCCCCCCCCEEEEEECCHHHHHHHH
FDALKSRTRGYASLDYEPAGEQASDLVKVDILLQGEAVDAFSAIVHREKAYSYGVAMTSK
HHHHHHHCCCCEECCCCCCCCCHHCEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHH
LRELIPRQQFEVPIQAAIGSRIIARENIRAIRKDVLAKCYGGDISRKRKLLEKQKEGKKR
HHHHCCHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
MKTIGRVEVPQEAFIAALSTTESPKAPAPKTGR
HHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
PDRPTSSETRPARVSEAPHEASGVDPASIRNFCIIAHIDHGKSTLADRMLGVTGVVEAR
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCEEEEEEECCCHHHHHHHHHHHHHHHHHH
NMRAQYLDRMDIERERGITIKAQNVRLPWRADDGQDYVLHLIDTPGHVDFTYEVSRSLAA
HHHHHHHHHHCCHHHCCCEEEECCEECCEECCCCCCEEEEEECCCCCEEEEHHHHHHHHH
CEGAVLLVDAAQGIEAQTLANLYLAIENDLAIIPVLNKIDLPAAQPEKCAAEISSIIGCD
HCCCEEEEECCCCCCHHHHHHEEEEEECCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCC
PGDVLKVSGKTGQGVPELLNEIVRRIPAPSGDPDGPLRAMIFDSVYDIYRGVITYVRVVD
CCCEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
GSLSTRDRCLMMSTGTSHETLEVGVISPEAQPTGSLSAGEVGYVIPGVKDVRQARVGDTI
CCCCCCCCEEEEECCCCCCEEEEEEECCCCCCCCCCCCCCCCEECCCHHHHHHHHCCCHH
TSARRPATEMLGGYRDPRPMVYSGLYPLDGSEYPALREALDKLQLNDAALTFEPETSAAL
HHHCCCHHHHHCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCHH
GFGFRCGFLGLLHLEIVRERLEREFDLTLISTAPNVVYRVVMEDTSEVTVTNPSDWPGGK
CCCHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCEEEEEECCCCEEEEECCCCCCCCC
IAEVYEPVVEAMLLLPTDFVGAVMELCQGRRGVLKGMDYISTDRVELKYTLPLGEIIFDF
HHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHCCCCCCCCCEEEEEECCHHHHHHHH
FDALKSRTRGYASLDYEPAGEQASDLVKVDILLQGEAVDAFSAIVHREKAYSYGVAMTSK
HHHHHHHCCCCEECCCCCCCCCHHCEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHH
LRELIPRQQFEVPIQAAIGSRIIARENIRAIRKDVLAKCYGGDISRKRKLLEKQKEGKKR
HHHHCCHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
MKTIGRVEVPQEAFIAALSTTESPKAPAPKTGR
HHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA