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The map label for this gene is gltD [H]

Identifier: 158313771

GI number: 158313771

Start: 2323968

End: 2325413

Strand: Direct

Name: gltD [H]

Synonym: Franean1_1936

Alternate gene names: 158313771

Gene position: 2323968-2325413 (Clockwise)

Preceding gene: 158313770

Following gene: 158313772

Centisome position: 25.87

GC content: 72.27

Gene sequence:

>1446_bases
ATGGCTGACCCGACCGGCTTTCTCAAGCACCGCCGGGAGCTGCCCACGCGCCGACCGGTGGACGTGCGCATCACCGACTG
GCGCGAGGTGTACGAGTCGTTCCCGGAGCCGGCGCTGCGTGACCAGGCGGGCCGGTGCATGGACTGCGGCATCCCGTTCT
GCCACGAGGGCTGCCCGCTCGGGAACATCATTCCCGAGTGGAACGACCTCGCCCGCCGCTCGGACTGGTCCGAGGCGATC
GAACGGCTGCACGCGACGAACAACTTCCCGGAGTTCACCGGGCGGCTGTGCCCCGCGCCGTGCGAGGCGGCCTGCGTGCT
GGGCATCGGGGACGACCCGGTGACCATCAAGCAGATCGAGGTCACCATCATCAACCGTGCCATCTCGGACGGGTCGGTCG
TGCCGGTGCCGCCGTCGGTGCGCACGGGCCGCCGCGTCGCGGTCGTCGGCTCGGGCCCGGCCGGCCTCGCCGCCGCCCAG
CAGCTCACCCGGGCCGGCCACGAGGTGGTCGTCTACGAGCGGGCCGACCGCGCCGGCGGCCTGCTCCGCTACGGCATCCC
CGAGTTCAAGATGGAGAAGCGGGTCCTCGACAACCGTCTGGCCCAGATGGAGGCCGAGGGGACGTCGTTCGTCACCTCGT
GCAACGTGGGCGTCGACATCACCGCCGACGAGCTGCGTTCGTCCTACGACGCGGTCGTCCTGGCCGGCGGGTCGACGGTG
GGGCGTGACCTTCCCGTCCCCGGGCGGGAGCTGCGGGGCATCCACCTGGCCATGGAGTTCCTGGTCCCGGCGAACCGGGT
GCAGCAGGGGGACCTGGCCGAGACGACGATCTCCGCTGCCGGCAAGCGGGTCGTGATCATCGGCGGTGGCGACACCGGGG
CGGACTGCCTGGGCACCTCGCACCGGCAGGGCGCGCTGTCCGTCCACCAGCTCGAGATCATGCCGAGGCCGCCCGCCTCC
CGGCCGCCGGCGAATCCGTGGCCGTCCTTCCCGATCGTCTACCGGGTGTCCAGTGCGCACGAGGAGGGCGGCGAGCGGGT
CTTCGCCGTCTCCACCGAGCGCTTCGTCGGCGATGACGAGGGCAACGTGCGGGCCCTGCTCATGCACGAGGTCCAGATGG
TCGACGGGCGCTTCCAGAAGATCGAGGGCAGCGAGACCGAGCTGCCGTGCGACCTGGTGCTGCTGGCGATGGGCTTCGTC
GGCCCGGAGCGCCCCGGCCTGCTCGAGCAGCTCGGCGTCGACCTGGACGCCCGCGGCAACGTCGCCCGCGATCTTGGTTG
GTCGACGTCGGTGCCCGGCGTGTTCGTGGCCGGTGACATGGGCCGTGGCCAGTCCCTGATCGTCTGGGCCATCGCGGAGG
GGCGCTCGGCGGCCGCCGCCGTCGACCGGCACCTGATGGGGACGACAGACCTGCCGGCTCCGATCCTGCCGACCCGCCGG
CCCTGA

Upstream 100 bases:

>100_bases
GACATCACGGCCGGGAACGCGATGCCGGCGTCCCCGAACCATCAGCGGGGCACGCGGGGCGGACACGTCACAGCGGCCTC
ATGGAACCGACAGGAGCGGC

Downstream 100 bases:

>100_bases
CCCCGAGCCCGGCCGGGCGTTCGCTCGGCCGGGCTCGATCGTCGTCGGCCCGGTCCGGGTGGGGCCATTGGACCCGTCCA
CCCGGCTCCACCCGTCCGTG

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: NADPH-GOGAT [H]

Number of amino acids: Translated: 481; Mature: 480

Protein sequence:

>481_residues
MADPTGFLKHRRELPTRRPVDVRITDWREVYESFPEPALRDQAGRCMDCGIPFCHEGCPLGNIIPEWNDLARRSDWSEAI
ERLHATNNFPEFTGRLCPAPCEAACVLGIGDDPVTIKQIEVTIINRAISDGSVVPVPPSVRTGRRVAVVGSGPAGLAAAQ
QLTRAGHEVVVYERADRAGGLLRYGIPEFKMEKRVLDNRLAQMEAEGTSFVTSCNVGVDITADELRSSYDAVVLAGGSTV
GRDLPVPGRELRGIHLAMEFLVPANRVQQGDLAETTISAAGKRVVIIGGGDTGADCLGTSHRQGALSVHQLEIMPRPPAS
RPPANPWPSFPIVYRVSSAHEEGGERVFAVSTERFVGDDEGNVRALLMHEVQMVDGRFQKIEGSETELPCDLVLLAMGFV
GPERPGLLEQLGVDLDARGNVARDLGWSTSVPGVFVAGDMGRGQSLIVWAIAEGRSAAAAVDRHLMGTTDLPAPILPTRR
P

Sequences:

>Translated_481_residues
MADPTGFLKHRRELPTRRPVDVRITDWREVYESFPEPALRDQAGRCMDCGIPFCHEGCPLGNIIPEWNDLARRSDWSEAI
ERLHATNNFPEFTGRLCPAPCEAACVLGIGDDPVTIKQIEVTIINRAISDGSVVPVPPSVRTGRRVAVVGSGPAGLAAAQ
QLTRAGHEVVVYERADRAGGLLRYGIPEFKMEKRVLDNRLAQMEAEGTSFVTSCNVGVDITADELRSSYDAVVLAGGSTV
GRDLPVPGRELRGIHLAMEFLVPANRVQQGDLAETTISAAGKRVVIIGGGDTGADCLGTSHRQGALSVHQLEIMPRPPAS
RPPANPWPSFPIVYRVSSAHEEGGERVFAVSTERFVGDDEGNVRALLMHEVQMVDGRFQKIEGSETELPCDLVLLAMGFV
GPERPGLLEQLGVDLDARGNVARDLGWSTSVPGVFVAGDMGRGQSLIVWAIAEGRSAAAAVDRHLMGTTDLPAPILPTRR
P
>Mature_480_residues
ADPTGFLKHRRELPTRRPVDVRITDWREVYESFPEPALRDQAGRCMDCGIPFCHEGCPLGNIIPEWNDLARRSDWSEAIE
RLHATNNFPEFTGRLCPAPCEAACVLGIGDDPVTIKQIEVTIINRAISDGSVVPVPPSVRTGRRVAVVGSGPAGLAAAQQ
LTRAGHEVVVYERADRAGGLLRYGIPEFKMEKRVLDNRLAQMEAEGTSFVTSCNVGVDITADELRSSYDAVVLAGGSTVG
RDLPVPGRELRGIHLAMEFLVPANRVQQGDLAETTISAAGKRVVIIGGGDTGADCLGTSHRQGALSVHQLEIMPRPPASR
PPANPWPSFPIVYRVSSAHEEGGERVFAVSTERFVGDDEGNVRALLMHEVQMVDGRFQKIEGSETELPCDLVLLAMGFVG
PERPGLLEQLGVDLDARGNVARDLGWSTSVPGVFVAGDMGRGQSLIVWAIAEGRSAAAAVDRHLMGTTDLPAPILPTRRP

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI111118981, Length=158, Percent_Identity=34.8101265822785, Blast_Score=76, Evalue=7e-14,
Organism=Homo sapiens, GI111118983, Length=158, Percent_Identity=34.8101265822785, Blast_Score=76, Evalue=8e-14,
Organism=Homo sapiens, GI119943098, Length=452, Percent_Identity=22.5663716814159, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1789606, Length=471, Percent_Identity=36.9426751592357, Blast_Score=263, Evalue=2e-71,
Organism=Escherichia coli, GI1788811, Length=464, Percent_Identity=35.3448275862069, Blast_Score=240, Evalue=2e-64,
Organism=Escherichia coli, GI87082180, Length=453, Percent_Identity=35.5408388520971, Blast_Score=211, Evalue=1e-55,
Organism=Escherichia coli, GI1788468, Length=441, Percent_Identity=26.0770975056689, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI1789243, Length=417, Percent_Identity=28.0575539568345, Blast_Score=120, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17570289, Length=490, Percent_Identity=47.5510204081633, Blast_Score=458, Evalue=1e-129,
Organism=Caenorhabditis elegans, GI17543860, Length=156, Percent_Identity=37.8205128205128, Blast_Score=86, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6320030, Length=485, Percent_Identity=47.6288659793814, Blast_Score=439, Evalue=1e-124,
Organism=Drosophila melanogaster, GI28574881, Length=487, Percent_Identity=51.7453798767967, Blast_Score=478, Evalue=1e-135,
Organism=Drosophila melanogaster, GI24665539, Length=487, Percent_Identity=51.7453798767967, Blast_Score=478, Evalue=1e-135,
Organism=Drosophila melanogaster, GI24665547, Length=487, Percent_Identity=51.7453798767967, Blast_Score=476, Evalue=1e-134,
Organism=Drosophila melanogaster, GI24665543, Length=487, Percent_Identity=51.7453798767967, Blast_Score=476, Evalue=1e-134,
Organism=Drosophila melanogaster, GI24640763, Length=483, Percent_Identity=23.6024844720497, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI18858217, Length=483, Percent_Identity=23.6024844720497, Blast_Score=77, Evalue=3e-14,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006005
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 51845; Mature: 51713

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADPTGFLKHRRELPTRRPVDVRITDWREVYESFPEPALRDQAGRCMDCGIPFCHEGCPL
CCCCHHHHHHHHHCCCCCCCEEEECCHHHHHHHCCCCHHHHHCCCEEECCCCHHCCCCCC
GNIIPEWNDLARRSDWSEAIERLHATNNFPEFTGRLCPAPCEAACVLGIGDDPVTIKQIE
CCCCCCHHHHHHCCCHHHHHHHHHHCCCCHHHHCCCCCCCCCCEEEEECCCCCEEEEEEE
VTIINRAISDGSVVPVPPSVRTGRRVAVVGSGPAGLAAAQQLTRAGHEVVVYERADRAGG
EEEEEEECCCCCEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCEEEEEECCCCCCC
LLRYGIPEFKMEKRVLDNRLAQMEAEGTSFVTSCNVGVDITADELRSSYDAVVLAGGSTV
EEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEHHHHHCCCCEEEEECCCCC
GRDLPVPGRELRGIHLAMEFLVPANRVQQGDLAETTISAAGKRVVIIGGGDTGADCLGTS
CCCCCCCCHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHCCCEEEEEECCCCCHHHCCCC
HRQGALSVHQLEIMPRPPASRPPANPWPSFPIVYRVSSAHEEGGERVFAVSTERFVGDDE
CCCCCCEEEEEEEECCCCCCCCCCCCCCCCCEEEEECCCCCCCCCEEEEEEHHHCCCCCC
GNVRALLMHEVQMVDGRFQKIEGSETELPCDLVLLAMGFVGPERPGLLEQLGVDLDARGN
CCEEEEEEHHHHHHCCHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCC
VARDLGWSTSVPGVFVAGDMGRGQSLIVWAIAEGRSAAAAVDRHLMGTTDLPAPILPTRR
CHHHCCCCCCCCCEEEECCCCCCCEEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCC
P
C
>Mature Secondary Structure 
ADPTGFLKHRRELPTRRPVDVRITDWREVYESFPEPALRDQAGRCMDCGIPFCHEGCPL
CCCHHHHHHHHHCCCCCCCEEEECCHHHHHHHCCCCHHHHHCCCEEECCCCHHCCCCCC
GNIIPEWNDLARRSDWSEAIERLHATNNFPEFTGRLCPAPCEAACVLGIGDDPVTIKQIE
CCCCCCHHHHHHCCCHHHHHHHHHHCCCCHHHHCCCCCCCCCCEEEEECCCCCEEEEEEE
VTIINRAISDGSVVPVPPSVRTGRRVAVVGSGPAGLAAAQQLTRAGHEVVVYERADRAGG
EEEEEEECCCCCEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCEEEEEECCCCCCC
LLRYGIPEFKMEKRVLDNRLAQMEAEGTSFVTSCNVGVDITADELRSSYDAVVLAGGSTV
EEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEHHHHHCCCCEEEEECCCCC
GRDLPVPGRELRGIHLAMEFLVPANRVQQGDLAETTISAAGKRVVIIGGGDTGADCLGTS
CCCCCCCCHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHCCCEEEEEECCCCCHHHCCCC
HRQGALSVHQLEIMPRPPASRPPANPWPSFPIVYRVSSAHEEGGERVFAVSTERFVGDDE
CCCCCCEEEEEEEECCCCCCCCCCCCCCCCCEEEEECCCCCCCCCEEEEEEHHHCCCCCC
GNVRALLMHEVQMVDGRFQKIEGSETELPCDLVLLAMGFVGPERPGLLEQLGVDLDARGN
CCEEEEEEHHHHHHCCHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCC
VARDLGWSTSVPGVFVAGDMGRGQSLIVWAIAEGRSAAAAVDRHLMGTTDLPAPILPTRR
CHHHCCCCCCCCCEEEECCCCCCCEEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCC
P
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11029411 [H]