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The map label for this gene is argF

Identifier: 158313577

GI number: 158313577

Start: 2081095

End: 2082048

Strand: Direct

Name: argF

Synonym: Franean1_1741

Alternate gene names: 158313577

Gene position: 2081095-2082048 (Clockwise)

Preceding gene: 158313576

Following gene: 158313578

Centisome position: 23.17

GC content: 71.91

Gene sequence:

>954_bases
ATGACGGCCCGACACTTCCTGCTCGACACCGACCTGACCTCGTCCGAGCAGGCCGCACTGCTGGATCTCGCCGACCAGCT
CAAGGCGACGCGCCGCGACCGGAAGCCCGCCCGCCGCCCGCTCGAGGGCCGCTCGGTCGCGCTGATCTTCGAGAAGCCGT
CGACCCGGACGAGGCTGTCCTTCGACGTTGCGGTGGCCGAGCTCGGCGGGCACCCGATCGTGATCGACTCCGGCTCCTCC
CAGCTCGGCCGCGGCGAGACCATCGAGGACACCGCAGCCGTCCTGAGCCGCTACGTCGACGCCATCGTCATCCGCACCTT
CGCCCAGGACAGGGTCGACCGGATGGCGGCGGCGGCCACCGTGCCCGTCATCAACGCGCTCTCCGACCACACCCACCCCT
GCCAGGCGCTCGCCGACCTGCAGACCATCCGGGAGATCCGCGGCCGGCTCGCCGGCGTGACCCTGGCGTACCTGGGCGAC
GGCAACAACGTCGCCCACTCGCTGCTGCTCGCCGGCGCACTCGCCGGGCTGCGGGTCCACGTCGCCTCGCCGCCGGGCTA
CGAGCCGATCGAGCAGGTCGTGCGGCACGCCAACGAGATCGGTGCGGTCACCGGCGGCGAGGCCCTGGTGACGCACGACG
CGCTCGAGGCCGCGGCCGGCGCGGACGTGCTCTACACCGACGTCTGGGCGTCGATGGGGCAGGACACCGAGTCCGACAGC
CGGGCGCTGGTCTTCCAGCCTTACCGCCTCGACGAGAAGGTCGTCGAGGCGGCCAGCCCGGACGCGATCGTCATGCACTG
CCTGCCCGCGCACCGGGAGATGGAGATCTCCGCGGCTGTGCTCGACGGGCCGCGTTCCGTGGTGTTCGACCAGGCGGAGA
ACCGGCTGCACGCGCAGAAGGCCCTGCTGTCGTTCCTGCTGGACGAGACCACGGCGGGTGTCGGTTCCCGATGA

Upstream 100 bases:

>100_bases
TGCCCGGCATCGCCGACCGGGCGCGGGAGACCCACGCCCAGGGGGACAGGACCGCGGCGACCGGCCCGAAAAAGTCGGAC
GAAGGATCTAAGGTCAGCGC

Downstream 100 bases:

>100_bases
CCATCCGCTCGGCCGCGCCGCTGACGAAACACGCCCGGCAGGCCCGCCTCGCCGCGCTGATCGCGGCGCGGTCGGTACGG
TCGCAGGCGGAGCTCGCGCG

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase

Number of amino acids: Translated: 317; Mature: 316

Protein sequence:

>317_residues
MTARHFLLDTDLTSSEQAALLDLADQLKATRRDRKPARRPLEGRSVALIFEKPSTRTRLSFDVAVAELGGHPIVIDSGSS
QLGRGETIEDTAAVLSRYVDAIVIRTFAQDRVDRMAAAATVPVINALSDHTHPCQALADLQTIREIRGRLAGVTLAYLGD
GNNVAHSLLLAGALAGLRVHVASPPGYEPIEQVVRHANEIGAVTGGEALVTHDALEAAAGADVLYTDVWASMGQDTESDS
RALVFQPYRLDEKVVEAASPDAIVMHCLPAHREMEISAAVLDGPRSVVFDQAENRLHAQKALLSFLLDETTAGVGSR

Sequences:

>Translated_317_residues
MTARHFLLDTDLTSSEQAALLDLADQLKATRRDRKPARRPLEGRSVALIFEKPSTRTRLSFDVAVAELGGHPIVIDSGSS
QLGRGETIEDTAAVLSRYVDAIVIRTFAQDRVDRMAAAATVPVINALSDHTHPCQALADLQTIREIRGRLAGVTLAYLGD
GNNVAHSLLLAGALAGLRVHVASPPGYEPIEQVVRHANEIGAVTGGEALVTHDALEAAAGADVLYTDVWASMGQDTESDS
RALVFQPYRLDEKVVEAASPDAIVMHCLPAHREMEISAAVLDGPRSVVFDQAENRLHAQKALLSFLLDETTAGVGSR
>Mature_316_residues
TARHFLLDTDLTSSEQAALLDLADQLKATRRDRKPARRPLEGRSVALIFEKPSTRTRLSFDVAVAELGGHPIVIDSGSSQ
LGRGETIEDTAAVLSRYVDAIVIRTFAQDRVDRMAAAATVPVINALSDHTHPCQALADLQTIREIRGRLAGVTLAYLGDG
NNVAHSLLLAGALAGLRVHVASPPGYEPIEQVVRHANEIGAVTGGEALVTHDALEAAAGADVLYTDVWASMGQDTESDSR
ALVFQPYRLDEKVVEAASPDAIVMHCLPAHREMEISAAVLDGPRSVVFDQAENRLHAQKALLSFLLDETTAGVGSR

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI38788445, Length=307, Percent_Identity=40.3908794788274, Blast_Score=236, Evalue=1e-62,
Organism=Homo sapiens, GI18105007, Length=315, Percent_Identity=32.0634920634921, Blast_Score=100, Evalue=1e-21,
Organism=Escherichia coli, GI1790703, Length=323, Percent_Identity=38.0804953560372, Blast_Score=210, Evalue=1e-55,
Organism=Escherichia coli, GI1786469, Length=332, Percent_Identity=37.6506024096386, Blast_Score=207, Evalue=5e-55,
Organism=Escherichia coli, GI2367364, Length=318, Percent_Identity=32.7044025157233, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI48994908, Length=352, Percent_Identity=27.8409090909091, Blast_Score=96, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI193204318, Length=310, Percent_Identity=31.9354838709677, Blast_Score=98, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6322373, Length=309, Percent_Identity=33.9805825242718, Blast_Score=191, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6322331, Length=313, Percent_Identity=31.629392971246, Blast_Score=105, Evalue=7e-24,
Organism=Drosophila melanogaster, GI24642586, Length=314, Percent_Identity=30.2547770700637, Blast_Score=114, Evalue=7e-26,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): OTC_FRASN (A8LE44)

Other databases:

- EMBL:   CP000820
- RefSeq:   YP_001506085.1
- ProteinModelPortal:   A8LE44
- SMR:   A8LE44
- GeneID:   5670143
- GenomeReviews:   CP000820_GR
- KEGG:   fre:Franean1_1741
- HOGENOM:   HBG579429
- OMA:   GATIHIS
- ProtClustDB:   PRK00779
- BioCyc:   FSP1855:FRANEAN1_1741-MONOMER
- HAMAP:   MF_01109
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292
- PRINTS:   PR00100
- PRINTS:   PR00102
- TIGRFAMs:   TIGR00658

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.3

Molecular weight: Translated: 33947; Mature: 33816

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: BINDING 105-105 BINDING 132-132

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTARHFLLDTDLTSSEQAALLDLADQLKATRRDRKPARRPLEGRSVALIFEKPSTRTRLS
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCHHCCCCCCCEEEEEEECCCCCEEEE
FDVAVAELGGHPIVIDSGSSQLGRGETIEDTAAVLSRYVDAIVIRTFAQDRVDRMAAAAT
HEEHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VPVINALSDHTHPCQALADLQTIREIRGRLAGVTLAYLGDGNNVAHSLLLAGALAGLRVH
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCEEEEEECCCCHHHHHHHHHHHHHCCEEE
VASPPGYEPIEQVVRHANEIGAVTGGEALVTHDALEAAAGADVLYTDVWASMGQDTESDS
ECCCCCCHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHCCCCHHHHHHHHHCCCCCCCCC
RALVFQPYRLDEKVVEAASPDAIVMHCLPAHREMEISAAVLDGPRSVVFDQAENRLHAQK
CEEEEECCCCCHHHHHHCCCCEEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHH
ALLSFLLDETTAGVGSR
HHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TARHFLLDTDLTSSEQAALLDLADQLKATRRDRKPARRPLEGRSVALIFEKPSTRTRLS
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCHHCCCCCCCEEEEEEECCCCCEEEE
FDVAVAELGGHPIVIDSGSSQLGRGETIEDTAAVLSRYVDAIVIRTFAQDRVDRMAAAAT
HEEHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VPVINALSDHTHPCQALADLQTIREIRGRLAGVTLAYLGDGNNVAHSLLLAGALAGLRVH
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCEEEEEECCCCHHHHHHHHHHHHHCCEEE
VASPPGYEPIEQVVRHANEIGAVTGGEALVTHDALEAAAGADVLYTDVWASMGQDTESDS
ECCCCCCHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHCCCCHHHHHHHHHCCCCCCCCC
RALVFQPYRLDEKVVEAASPDAIVMHCLPAHREMEISAAVLDGPRSVVFDQAENRLHAQK
CEEEEECCCCCHHHHHHCCCCEEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHH
ALLSFLLDETTAGVGSR
HHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA