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The map label for this gene is sphR [H]

Identifier: 158313449

GI number: 158313449

Start: 1929687

End: 1930466

Strand: Direct

Name: sphR [H]

Synonym: Franean1_1612

Alternate gene names: 158313449

Gene position: 1929687-1930466 (Clockwise)

Preceding gene: 158313448

Following gene: 158313450

Centisome position: 21.48

GC content: 75.51

Gene sequence:

>780_bases
GTGGACACCCACACGCAGATGGACGCGGACAGCCACGCTCCCGAGCTTGTCCTCGTTGTCGAGGACGAGGCGCACATCGC
CGAGTTGATCCGCATGTACCTCGTCCGGGAGGGGTTCGCCGTCGAGGTCCGCGCCGACGGTCCGGCCGGCCTGGCGGCGG
CGCGTGCCCTCACCCCGGTCGCGGTCATCCTGGACGTCCGCCTGCCCGGCCTGGACGGCCGTGAGCTGCTGCGGGCCCTG
CGCGCCGACGGTGACTGGGTGCCGGTGCTGCTGGTGACGGCCCGCGACGACGAGGTCGACCGCGTCGTCGGCCTGGAACT
GGGCGCCGACGACTACGTCACGAAGCCGTTCTCGCCCCGCGAGCTGGTCGCGCGGCTGCGCGCCGTCCTGCGGCGCACCA
GCGGGCCCGCCGCGCTCGCACGGTCGACCGACGCCGCCGGTGCGCCTGCCGGTCCTGGTGCCGGTGCCGGTCCTGACGCC
AATGCCGGCGGCCCGGTGCTGCGCGCCGGGGTCGTCGTGCTTGACGTCGGGCGGCGGCGGGTGCTCGCCGGCGACCGCGA
GGTGACCCTCACGACCACCGAGTTCGACCTGCTCGCGGCCCTGCTGCGCCGTCCCGGCCTGGTCCACACCCGGGACCGCC
TGCTCGCCGACGTGTGGGGCTACCAGGCGAGCGCCGGCACCCGGACCGTCGATGTGCACGTGGCCCAGGTCCGGGCGAAA
CTCGGCCCGGCCAGCCCGATCCGCACCGTCCGCGGCGTCGGGTACACCGCCGATGCCTGA

Upstream 100 bases:

>100_bases
ACCATGTCCCAGTCCCGGCCCGTCCCGCGAGTGGCCCGCCGTCCGGCCGTCCCGCCCCCGGCCAGCCGGCTTCCGTTCCC
GTGTCCGCACACTCACTGAG

Downstream 100 bases:

>100_bases
CCCCGGGAGCCGCCCGAACCCGCGGAATCGGCGGCCCGCCGGGCTGGCGGTGCGGATCGCCCTGGCGACGACCGCCGTCG
CCGCGCTCGCGGTCCTGCTC

Product: two component transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MDTHTQMDADSHAPELVLVVEDEAHIAELIRMYLVREGFAVEVRADGPAGLAAARALTPVAVILDVRLPGLDGRELLRAL
RADGDWVPVLLVTARDDEVDRVVGLELGADDYVTKPFSPRELVARLRAVLRRTSGPAALARSTDAAGAPAGPGAGAGPDA
NAGGPVLRAGVVVLDVGRRRVLAGDREVTLTTTEFDLLAALLRRPGLVHTRDRLLADVWGYQASAGTRTVDVHVAQVRAK
LGPASPIRTVRGVGYTADA

Sequences:

>Translated_259_residues
MDTHTQMDADSHAPELVLVVEDEAHIAELIRMYLVREGFAVEVRADGPAGLAAARALTPVAVILDVRLPGLDGRELLRAL
RADGDWVPVLLVTARDDEVDRVVGLELGADDYVTKPFSPRELVARLRAVLRRTSGPAALARSTDAAGAPAGPGAGAGPDA
NAGGPVLRAGVVVLDVGRRRVLAGDREVTLTTTEFDLLAALLRRPGLVHTRDRLLADVWGYQASAGTRTVDVHVAQVRAK
LGPASPIRTVRGVGYTADA
>Mature_259_residues
MDTHTQMDADSHAPELVLVVEDEAHIAELIRMYLVREGFAVEVRADGPAGLAAARALTPVAVILDVRLPGLDGRELLRAL
RADGDWVPVLLVTARDDEVDRVVGLELGADDYVTKPFSPRELVARLRAVLRRTSGPAALARSTDAAGAPAGPGAGAGPDA
NAGGPVLRAGVVVLDVGRRRVLAGDREVTLTTTEFDLLAALLRRPGLVHTRDRLLADVWGYQASAGTRTVDVHVAQVRAK
LGPASPIRTVRGVGYTADA

Specific function: Member of the two-component regulatory system sphR/sphS. Response regulator. Involved in inducible production of alkaline phosphatase in response to phosphate limitation as it is directly involved in the regulation of phoA transcription in response to pho

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790860, Length=246, Percent_Identity=42.2764227642276, Blast_Score=158, Evalue=4e-40,
Organism=Escherichia coli, GI1786599, Length=244, Percent_Identity=37.2950819672131, Blast_Score=145, Evalue=3e-36,
Organism=Escherichia coli, GI1789809, Length=246, Percent_Identity=37.8048780487805, Blast_Score=144, Evalue=5e-36,
Organism=Escherichia coli, GI1789402, Length=240, Percent_Identity=38.3333333333333, Blast_Score=140, Evalue=8e-35,
Organism=Escherichia coli, GI1788394, Length=259, Percent_Identity=34.3629343629344, Blast_Score=138, Evalue=3e-34,
Organism=Escherichia coli, GI1790552, Length=239, Percent_Identity=33.8912133891213, Blast_Score=124, Evalue=6e-30,
Organism=Escherichia coli, GI2367329, Length=243, Percent_Identity=34.9794238683128, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI87082012, Length=243, Percent_Identity=34.9794238683128, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1786784, Length=242, Percent_Identity=34.7107438016529, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1786911, Length=245, Percent_Identity=37.1428571428571, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1790863, Length=244, Percent_Identity=31.1475409836066, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1787229, Length=244, Percent_Identity=36.0655737704918, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1787375, Length=242, Percent_Identity=30.5785123966942, Blast_Score=103, Evalue=9e-24,
Organism=Escherichia coli, GI145693140, Length=244, Percent_Identity=30.327868852459, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1788550, Length=106, Percent_Identity=32.0754716981132, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1788191, Length=119, Percent_Identity=35.2941176470588, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 27328; Mature: 27328

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTHTQMDADSHAPELVLVVEDEAHIAELIRMYLVREGFAVEVRADGPAGLAAARALTPV
CCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHE
AVILDVRLPGLDGRELLRALRADGDWVPVLLVTARDDEVDRVVGLELGADDYVTKPFSPR
EEEEEEECCCCCHHHHHHHHHCCCCEEEEEEEEECCCCCCCEEEEEECCCCCCCCCCCHH
ELVARLRAVLRRTSGPAALARSTDAAGAPAGPGAGAGPDANAGGPVLRAGVVVLDVGRRR
HHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCEEEEECCCCE
VLAGDREVTLTTTEFDLLAALLRRPGLVHTRDRLLADVWGYQASAGTRTVDVHVAQVRAK
EECCCCEEEEEECHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHH
LGPASPIRTVRGVGYTADA
CCCCCHHHHHHCCCCCCCC
>Mature Secondary Structure
MDTHTQMDADSHAPELVLVVEDEAHIAELIRMYLVREGFAVEVRADGPAGLAAARALTPV
CCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHE
AVILDVRLPGLDGRELLRALRADGDWVPVLLVTARDDEVDRVVGLELGADDYVTKPFSPR
EEEEEEECCCCCHHHHHHHHHCCCCEEEEEEEEECCCCCCCEEEEEECCCCCCCCCCCHH
ELVARLRAVLRRTSGPAALARSTDAAGAPAGPGAGAGPDANAGGPVLRAGVVVLDVGRRR
HHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCEEEEECCCCE
VLAGDREVTLTTTEFDLLAALLRRPGLVHTRDRLLADVWGYQASAGTRTVDVHVAQVRAK
EECCCCEEEEEECHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHH
LGPASPIRTVRGVGYTADA
CCCCCHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8497200; 8157591 [H]