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The map label for this gene is tfdA [H]

Identifier: 158313257

GI number: 158313257

Start: 1711641

End: 1712471

Strand: Direct

Name: tfdA [H]

Synonym: Franean1_1415

Alternate gene names: 158313257

Gene position: 1711641-1712471 (Clockwise)

Preceding gene: 158313256

Following gene: 158313259

Centisome position: 19.06

GC content: 67.27

Gene sequence:

>831_bases
ATGGCGGTCCTGACACTCAACAAGCTGAGCACGCACGTCGGCGCCGAGATCCTCGGCCTCGACGCGACCCGATTGTTATC
GGACGAGACACTGCCCGGAGCCGTCCTGGACGCCCTCGAAGAGAACGGCGTGCTCGTCTTCCGGAACCTTCATCTCGACG
ACGCCACCCAGGTCGCGTTCTGCCGGAAGCTCGGGGCGGTCCAGAGCTGGGCCAGTCACGCGATTCCGGAGATCACCGTG
GTCAGTCTGGACCCGGCGAAGACAGCCATCGCCGAGTATCTGAAGGGCACGTTCGACTGGCACATCGACGGAACCGTCGA
CGTGATCCCGAACAAGGCGACAGTCCTCACCGCGCACACGCTCGCGGAGGAGGGCGGGCAGACCGAGTTCGCCAGCACCT
ACGTCGCCTACGAGAATCTCACCGCCGAGGAGAAGGAGCAGTACGCACAGCTTCGTGTGCTGCACAGTTTCGAGGCACTC
CAGCTGCGCAGGAACCCGAATCCGACGCCGGAGGTCGCGGCGGACATGAAGTCCCGCCCCCAGCGCGAGCATCCGCTGAT
CTGGCGCCACGGCTCGGGGCGCAACTCGCTGGTGCTCGGGGCGACCGCCTCCCACGTCGTCGGCTGGGATGACGCGGCCA
GCCAGCAGCTCCTCGACGGCCTGCTCGCCCGCTCCACCGCGCCGCAACTGGTGTACCGGCACGAGTGGTCCCTGGGCGAC
ACCGTGATCTGGGACAACCGCGGCGTCCTGCACCGTGTCAGCCCCTACGACCCCACGTCGCCCCGGGAGATGCACCGCTC
GACGCTCCTCGGGGACGAACCAATCCAGTGA

Upstream 100 bases:

>100_bases
CCGCAGTCGGCACAGCGGCGTGCACGACACAGCGACCGCCGGACCTGGGTAAGCCTAGTCAAGCTGGACGTTCACCAGCT
CGACCGGCGAGAGGAGCACG

Downstream 100 bases:

>100_bases
CGCCTGCAGCGAGCGCTCGGCGGGCGGCCGATCCGGTCAGCAGGCTCCGCCGGCACCGGCCGCCGGGTACTCACGATCGC
TGTCACGACGTCGGCGCATC

Product: taurine catabolism dioxygenase TauD/TfdA

Products: Aminoacetaldehyde; CO2; H+; Sulfite; Succinate [C]

Alternate protein names: 2,4-D dioxygenase [H]

Number of amino acids: Translated: 276; Mature: 275

Protein sequence:

>276_residues
MAVLTLNKLSTHVGAEILGLDATRLLSDETLPGAVLDALEENGVLVFRNLHLDDATQVAFCRKLGAVQSWASHAIPEITV
VSLDPAKTAIAEYLKGTFDWHIDGTVDVIPNKATVLTAHTLAEEGGQTEFASTYVAYENLTAEEKEQYAQLRVLHSFEAL
QLRRNPNPTPEVAADMKSRPQREHPLIWRHGSGRNSLVLGATASHVVGWDDAASQQLLDGLLARSTAPQLVYRHEWSLGD
TVIWDNRGVLHRVSPYDPTSPREMHRSTLLGDEPIQ

Sequences:

>Translated_276_residues
MAVLTLNKLSTHVGAEILGLDATRLLSDETLPGAVLDALEENGVLVFRNLHLDDATQVAFCRKLGAVQSWASHAIPEITV
VSLDPAKTAIAEYLKGTFDWHIDGTVDVIPNKATVLTAHTLAEEGGQTEFASTYVAYENLTAEEKEQYAQLRVLHSFEAL
QLRRNPNPTPEVAADMKSRPQREHPLIWRHGSGRNSLVLGATASHVVGWDDAASQQLLDGLLARSTAPQLVYRHEWSLGD
TVIWDNRGVLHRVSPYDPTSPREMHRSTLLGDEPIQ
>Mature_275_residues
AVLTLNKLSTHVGAEILGLDATRLLSDETLPGAVLDALEENGVLVFRNLHLDDATQVAFCRKLGAVQSWASHAIPEITVV
SLDPAKTAIAEYLKGTFDWHIDGTVDVIPNKATVLTAHTLAEEGGQTEFASTYVAYENLTAEEKEQYAQLRVLHSFEALQ
LRRNPNPTPEVAADMKSRPQREHPLIWRHGSGRNSLVLGATASHVVGWDDAASQQLLDGLLARSTAPQLVYRHEWSLGDT
VIWDNRGVLHRVSPYDPTSPREMHRSTLLGDEPIQ

Specific function: Involved in degradation of the herbicide 2,4- dichlorophenoxyacetic acid (2,4-D). Is also able to degrade 2- methyl-4-chlorophenoxyacetic acid and 3-chlorobenzoic acid [H]

COG id: COG2175

COG function: function code Q; Probable taurine catabolism dioxygenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tfdA dioxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786565, Length=280, Percent_Identity=27.5, Blast_Score=92, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003819 [H]

Pfam domain/function: PF02668 TauD [H]

EC number: 1.14.11.17 [C]

Molecular weight: Translated: 30389; Mature: 30258

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVLTLNKLSTHVGAEILGLDATRLLSDETLPGAVLDALEENGVLVFRNLHLDDATQVAF
CEEEEEHHHHHHHCCEEECCCHHHHHCCCCCCHHHHHHHHCCCEEEEEECCCCCHHHHHH
CRKLGAVQSWASHAIPEITVVSLDPAKTAIAEYLKGTFDWHIDGTVDVIPNKATVLTAHT
HHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHCCCEEEEECCEEEECCCCEEEEEEHH
LAEEGGQTEFASTYVAYENLTAEEKEQYAQLRVLHSFEALQLRRNPNPTPEVAADMKSRP
HHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCC
QREHPLIWRHGSGRNSLVLGATASHVVGWDDAASQQLLDGLLARSTAPQLVYRHEWSLGD
CCCCCEEEEECCCCCCEEEECCCCCEECCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCC
TVIWDNRGVLHRVSPYDPTSPREMHRSTLLGDEPIQ
EEEECCCCCEEECCCCCCCCHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
AVLTLNKLSTHVGAEILGLDATRLLSDETLPGAVLDALEENGVLVFRNLHLDDATQVAF
EEEEEHHHHHHHCCEEECCCHHHHHCCCCCCHHHHHHHHCCCEEEEEECCCCCHHHHHH
CRKLGAVQSWASHAIPEITVVSLDPAKTAIAEYLKGTFDWHIDGTVDVIPNKATVLTAHT
HHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHCCCEEEEECCEEEECCCCEEEEEEHH
LAEEGGQTEFASTYVAYENLTAEEKEQYAQLRVLHSFEALQLRRNPNPTPEVAADMKSRP
HHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCC
QREHPLIWRHGSGRNSLVLGATASHVVGWDDAASQQLLDGLLARSTAPQLVYRHEWSLGD
CCCCCEEEEECCCCCCEEEECCCCCEECCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCC
TVIWDNRGVLHRVSPYDPTSPREMHRSTLLGDEPIQ
EEEECCCCCEEECCCCCCCCHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Ascorbate. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-Oxoglutarate; O2; Taurine [C]

Specific reaction: 2-Oxoglutarate + O2 + Taurine --> Aminoacetaldehyde + CO2 + H+ + Sulfite + Succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8779585 [H]