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The map label for this gene is dapF [H]

Identifier: 158313082

GI number: 158313082

Start: 1471573

End: 1472496

Strand: Direct

Name: dapF [H]

Synonym: Franean1_1230

Alternate gene names: 158313082

Gene position: 1471573-1472496 (Clockwise)

Preceding gene: 158313081

Following gene: 158313083

Centisome position: 16.38

GC content: 75.43

Gene sequence:

>924_bases
GTGAGCTCGCCGCGGCCGTACGATCGCGGCGTGCGCTTCGTCAAGGGACACGGAACGGGTAACGACTTCCTCATCCTGCC
GGATGCTGACGGCGAGCTCGATCTCAGCCCCGAGCTGGTGCGGGCGCTGTGCCACCGAGGCACCGGCATTGGCGCCGACG
GGGTGCTGCGGGTCGTGTTGACCGCGGCCGAGCCCGCCGCCGGCGACAGCGCGGCCGCCCGCTGGTTCATGGACTATCGC
AACGCCGACGGGTCGATCGCCGAGATGTGCGGCAACGGGATCCGGGTGTTCGCCCGCTATCTCGTCGACGCGGGTTACGC
CGCGCCCGGCCGTCTCGTCATCGCCACCCGGGCCGGCCTGCGGGCGGTCGAGGTGCCTGCGGGCGGCGACGTCACCGTCG
AGATGGGACCGCCCGAGCTGGCCGCCGGCCCGGTGCCGGTCACGGTCGCCGGGCGGGAGTTCGCCGCCGTGCCGGTCTCG
ATGGGGAACCCGCACGCGGTCTGCTTCGCCGACGACCTCGACCCGGCCGGCCTGGCGCGCCTGGACCTGCGCGCGCCGGC
CTACCCGGCCGCCGCCTTCCCCGAGGGCGTGAATGTCGAGGTCGTCGTCCCGGCTCCGGACGGGGTCGCCATGCGGGTCT
TCGAGCGGGGCGTCGGTGAGACCGCGTCCTGCGGCACCGGCGCGTGTGCCGTCGCTGTGGCCTGGGCGGCCCGCAATGGC
GATGGGCCGCCCGCAGAGGTCGAGGTCGACCTCCCCGGCGGGCGGCTGACTGTCGGCTGGCGGGAGTCGACCGTCCTGCT
CAGTGGCCCGGCCGTGCTGGTGTCGGACGGTGTCCTTCGTCCCGACTGGCTGCGCGCGGCGGGTGGCTTCGGCCCGGCGG
CTCCCGCGGGCGAGCGGCGTCCGAGCGCTGTCGGGGCCGGCTGA

Upstream 100 bases:

>100_bases
CCGCCCAGCCGACGCGTTCGGGAGGTACCGCGCAGTCAGGAGATACCGCGTATCCGGGTGGTTCGGGGCGAGGGGACCGT
TCGCGGGCGGCTGCCGCGGG

Downstream 100 bases:

>100_bases
GCGCGGCCGAGGCAGGTTGAGCGCGGCCGGGGCAGGCAGGGCGAAGTCCCTCGATCTCCCTCGGGGACCCGTAGCGCGTG
GGTGCGCCCGAGCGTCGGGC

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase [H]

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MSSPRPYDRGVRFVKGHGTGNDFLILPDADGELDLSPELVRALCHRGTGIGADGVLRVVLTAAEPAAGDSAAARWFMDYR
NADGSIAEMCGNGIRVFARYLVDAGYAAPGRLVIATRAGLRAVEVPAGGDVTVEMGPPELAAGPVPVTVAGREFAAVPVS
MGNPHAVCFADDLDPAGLARLDLRAPAYPAAAFPEGVNVEVVVPAPDGVAMRVFERGVGETASCGTGACAVAVAWAARNG
DGPPAEVEVDLPGGRLTVGWRESTVLLSGPAVLVSDGVLRPDWLRAAGGFGPAAPAGERRPSAVGAG

Sequences:

>Translated_307_residues
MSSPRPYDRGVRFVKGHGTGNDFLILPDADGELDLSPELVRALCHRGTGIGADGVLRVVLTAAEPAAGDSAAARWFMDYR
NADGSIAEMCGNGIRVFARYLVDAGYAAPGRLVIATRAGLRAVEVPAGGDVTVEMGPPELAAGPVPVTVAGREFAAVPVS
MGNPHAVCFADDLDPAGLARLDLRAPAYPAAAFPEGVNVEVVVPAPDGVAMRVFERGVGETASCGTGACAVAVAWAARNG
DGPPAEVEVDLPGGRLTVGWRESTVLLSGPAVLVSDGVLRPDWLRAAGGFGPAAPAGERRPSAVGAG
>Mature_306_residues
SSPRPYDRGVRFVKGHGTGNDFLILPDADGELDLSPELVRALCHRGTGIGADGVLRVVLTAAEPAAGDSAAARWFMDYRN
ADGSIAEMCGNGIRVFARYLVDAGYAAPGRLVIATRAGLRAVEVPAGGDVTVEMGPPELAAGPVPVTVAGREFAAVPVSM
GNPHAVCFADDLDPAGLARLDLRAPAYPAAAFPEGVNVEVVVPAPDGVAMRVFERGVGETASCGTGACAVAVAWAARNGD
GPPAEVEVDLPGGRLTVGWRESTVLLSGPAVLVSDGVLRPDWLRAAGGFGPAAPAGERRPSAVGAG

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family [H]

Homologues:

Organism=Escherichia coli, GI87082334, Length=285, Percent_Identity=36.140350877193, Blast_Score=138, Evalue=5e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001653
- InterPro:   IPR018510 [H]

Pfam domain/function: PF01678 DAP_epimerase [H]

EC number: =5.1.1.7 [H]

Molecular weight: Translated: 31223; Mature: 31091

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSPRPYDRGVRFVKGHGTGNDFLILPDADGELDLSPELVRALCHRGTGIGADGVLRVVL
CCCCCCHHHCCEEEEECCCCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEEE
TAAEPAAGDSAAARWFMDYRNADGSIAEMCGNGIRVFARYLVDAGYAAPGRLVIATRAGL
EECCCCCCCHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHCCCCCCCEEEEEECCCC
RAVEVPAGGDVTVEMGPPELAAGPVPVTVAGREFAAVPVSMGNPHAVCFADDLDPAGLAR
EEEEECCCCCEEEEECCCCCCCCCEEEEECCCCEEEEEEECCCCEEEEEECCCCCCCEEE
LDLRAPAYPAAAFPEGVNVEVVVPAPDGVAMRVFERGVGETASCGTGACAVAVAWAARNG
EECCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCC
DGPPAEVEVDLPGGRLTVGWRESTVLLSGPAVLVSDGVLRPDWLRAAGGFGPAAPAGERR
CCCCCEEEEECCCCEEEEEECCCEEEEECCEEEEECCCCCHHHHHHCCCCCCCCCCCCCC
PSAVGAG
CCCCCCC
>Mature Secondary Structure 
SSPRPYDRGVRFVKGHGTGNDFLILPDADGELDLSPELVRALCHRGTGIGADGVLRVVL
CCCCCHHHCCEEEEECCCCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEEE
TAAEPAAGDSAAARWFMDYRNADGSIAEMCGNGIRVFARYLVDAGYAAPGRLVIATRAGL
EECCCCCCCHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHCCCCCCCEEEEEECCCC
RAVEVPAGGDVTVEMGPPELAAGPVPVTVAGREFAAVPVSMGNPHAVCFADDLDPAGLAR
EEEEECCCCCEEEEECCCCCCCCCEEEEECCCCEEEEEEECCCCEEEEEECCCCCCCEEE
LDLRAPAYPAAAFPEGVNVEVVVPAPDGVAMRVFERGVGETASCGTGACAVAVAWAARNG
EECCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCC
DGPPAEVEVDLPGGRLTVGWRESTVLLSGPAVLVSDGVLRPDWLRAAGGFGPAAPAGERR
CCCCCEEEEECCCCEEEEEECCCEEEEECCEEEEECCCCCHHHHHHCCCCCCCCCCCCCC
PSAVGAG
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12000953 [H]