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The map label for this gene is pnp [H]

Identifier: 158313041

GI number: 158313041

Start: 1413593

End: 1415782

Strand: Direct

Name: pnp [H]

Synonym: Franean1_1189

Alternate gene names: 158313041

Gene position: 1413593-1415782 (Clockwise)

Preceding gene: 158313040

Following gene: 158313042

Centisome position: 15.74

GC content: 71.05

Gene sequence:

>2190_bases
GTGGACGACTCGACACAGGCGGCAGAGGCCGTCATCACGACCCCGACCGGGGCCCGCACGGTCCGTTTCGAGACCGGCCG
GCTGGCCAGGCAGGCCGCTGGCGCGGTCGTCGCCCACCTCGGCGACACGATGGTCCTCTCGGCGACGACGGTCAGCCGCT
CGCCGAAGGAGCAGCTGGACTTCTTCCCCCTGACGGTGGACGTCGAGGAGCGGATGTACGCGGCCGGGCGCATCCCCGGC
TCGTTCTTCCGCCGCGAGGGCCGCCCCAGTGAGGACGCGATCCTCACCTGCCGGCTCATCGACCGGCCGTTGCGGCCGTC
GTTCGCCAAGGGGCTGCGCAACGAGATCCAGGTCGTGGCGACCGTGCTCGCCCTCGACCCCGACACCCTGTACGACGTGG
TCGCGATCAACGCGGCGAGCGCGTCGACGATGCTGGCCGGGCTGCCCTTCAGCGGGCCGATCGGCGCCACCCGGGTCGGC
TACGTCGACGGCGACTGGATCGCCTTCCCGACGCACAGCGAGCTGGAGCGGGCGACCTTCGACATGGTCGTGGCCGGCCG
GGTGCTCGAGGACGGCTCCGACGTCGCGATCATGATGGTCGAGGCGGAGGCGACGCCGGGCACGGTGGCCCTGGTGGCCG
GTGGCGCGCCGGCGCCGACCGAGGAGGTCGTCGCCGCGGGCCTGGAGGCCGCCAAGCCGGCCATCCGCGAGCTGTGCCGG
GCGCAGAGCGAGCTGGCCGCGGGCGCGGCGAAGCCGCAGCGCGAGTTCCCGCTGTTCATCGACTACCACGACGACGTGCT
GGAGACCCTGACGGCGGCGGTCGGCGAGGAGCTTGCGGCGGCACTGCGCATCGCCGGCAAGGCCGAGCGCGAGACCGAGC
TGGAGCGGGTCCGGGCGCTGGCCGTGGAGAAGGTGTCCGGCCAGTTCGAGGGCCGCGACAAGGAGATCAGCCCGGCCTAC
CGGGCGCTGACCAAGAAGCTGGTCCGCTCGCGCATCGTCACCGACGGTGTGCGCATCGACGGGCGTTCCACGACCGAGAT
CCGCGAGCTGTCGGCCGAGGTCGACTACATCCCGCGGGTGCACGGCTCGGCGCTGTTCGAACGGGGCGAGACGCAGATCC
TCGGCGTCACGACGCTGGCGATGCTCCGGATGGAGCAGACCGTCGACACGCTCAACCCGGACCGCACCAAGCGGTACATG
CACAACTACAACTTCCCGCCGTACTCCACCGGTGAGACCGGCCGGGTGGGCTCGCCGAAGCGGCGCGAGATCGGCCACGG
CGCGCTCGCGGAGCGGGCGCTGCTGCCGGTGCTGCCCAGCCGCGAGGAGTTCCCCTACGCGATCCGCCAGGTCTCCGAGG
CGCTGAGCTCCAACGGCTCGACGAGCATGGGCTCGGTCTGCGCGAGCACGCTGTCGCTGCTGAACGCCGGTGTGCCGCTG
AAGGCCCCGGTCGCGGGCATCGCGATGGGCCTGATCCGGGAGGGCGACGCCTTCGTCACCCTGACCGACATCCTCGGGGC
AGAGGACGCCTACGGCGACATGGACTTCAAGGTCGCCGGCACGCAGGAGTTCGTGACCGCCCTGCAGCTGGACACCAAGC
TCGACGGCATCCCGGCGTCGGTGCTCGCCGGCGCGCTGCAGCAGGCGCGGGCGGCCCGGCTGACGATCCTCGAGGTGATG
GCCGAGGCCATCGGCGGGCCGGACGAGATGTCCGCGCACGCCCCGCGGGTCATCTCCGTGAAGATCCCCGTTGACAAGAT
CGGCGAGGTCATCGGGCCCAAGGGCAAGATGATCAACCAGATCCAGGCGGACTCCGGTGCGGAGATCACCGTCGAGGACG
ACGGCACGATCTACATCGGTGCCGCCGACGGGCCGGCGGCCGAGACCGCCCGCTCCGCGATCAACGCGATCGCGAACCCG
CAGATGCCCGAGGTCGGCGAGCGCTACCTCGGTACCATCGTGAAGATCACGAACTTCGGAGCCTTCGTCTCGCTGACGCC
CGGCAAGGACGGCCTGCTGCACGTCAGCAAGCTCAAGCAGCTCGCCGGCGGCAAGCGCGTCGAAAAGGTCGAGGACGTGC
TGTCGGTGGGCCAGAAGCTCCAGGTGGAGATCACCGAGATCGACGCCCGCGGCAAGATCAGCCTCGCGCCGGGCGCGGAG
TCCATCGACACCGTAAGCGCCGGATCCTGA

Upstream 100 bases:

>100_bases
GCCGCCGGGACGAGCGCCGTCGGTGCCGCCCGGAGACTTCGACTGAGCACCGGCATCCTCCATGGCGCGGGCACGGCCCA
CGATGAGGATGGAGTATCGA

Downstream 100 bases:

>100_bases
CAGGAGCGCAGTGACGGAGACGGATCTGTCCACCTCGGACGACCGCACCTCGGACGGTCGCACCTCGAACGACCGCACCC
CGGGCCTGGCCGGGCCGGAG

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 729; Mature: 729

Protein sequence:

>729_residues
MDDSTQAAEAVITTPTGARTVRFETGRLARQAAGAVVAHLGDTMVLSATTVSRSPKEQLDFFPLTVDVEERMYAAGRIPG
SFFRREGRPSEDAILTCRLIDRPLRPSFAKGLRNEIQVVATVLALDPDTLYDVVAINAASASTMLAGLPFSGPIGATRVG
YVDGDWIAFPTHSELERATFDMVVAGRVLEDGSDVAIMMVEAEATPGTVALVAGGAPAPTEEVVAAGLEAAKPAIRELCR
AQSELAAGAAKPQREFPLFIDYHDDVLETLTAAVGEELAAALRIAGKAERETELERVRALAVEKVSGQFEGRDKEISPAY
RALTKKLVRSRIVTDGVRIDGRSTTEIRELSAEVDYIPRVHGSALFERGETQILGVTTLAMLRMEQTVDTLNPDRTKRYM
HNYNFPPYSTGETGRVGSPKRREIGHGALAERALLPVLPSREEFPYAIRQVSEALSSNGSTSMGSVCASTLSLLNAGVPL
KAPVAGIAMGLIREGDAFVTLTDILGAEDAYGDMDFKVAGTQEFVTALQLDTKLDGIPASVLAGALQQARAARLTILEVM
AEAIGGPDEMSAHAPRVISVKIPVDKIGEVIGPKGKMINQIQADSGAEITVEDDGTIYIGAADGPAAETARSAINAIANP
QMPEVGERYLGTIVKITNFGAFVSLTPGKDGLLHVSKLKQLAGGKRVEKVEDVLSVGQKLQVEITEIDARGKISLAPGAE
SIDTVSAGS

Sequences:

>Translated_729_residues
MDDSTQAAEAVITTPTGARTVRFETGRLARQAAGAVVAHLGDTMVLSATTVSRSPKEQLDFFPLTVDVEERMYAAGRIPG
SFFRREGRPSEDAILTCRLIDRPLRPSFAKGLRNEIQVVATVLALDPDTLYDVVAINAASASTMLAGLPFSGPIGATRVG
YVDGDWIAFPTHSELERATFDMVVAGRVLEDGSDVAIMMVEAEATPGTVALVAGGAPAPTEEVVAAGLEAAKPAIRELCR
AQSELAAGAAKPQREFPLFIDYHDDVLETLTAAVGEELAAALRIAGKAERETELERVRALAVEKVSGQFEGRDKEISPAY
RALTKKLVRSRIVTDGVRIDGRSTTEIRELSAEVDYIPRVHGSALFERGETQILGVTTLAMLRMEQTVDTLNPDRTKRYM
HNYNFPPYSTGETGRVGSPKRREIGHGALAERALLPVLPSREEFPYAIRQVSEALSSNGSTSMGSVCASTLSLLNAGVPL
KAPVAGIAMGLIREGDAFVTLTDILGAEDAYGDMDFKVAGTQEFVTALQLDTKLDGIPASVLAGALQQARAARLTILEVM
AEAIGGPDEMSAHAPRVISVKIPVDKIGEVIGPKGKMINQIQADSGAEITVEDDGTIYIGAADGPAAETARSAINAIANP
QMPEVGERYLGTIVKITNFGAFVSLTPGKDGLLHVSKLKQLAGGKRVEKVEDVLSVGQKLQVEITEIDARGKISLAPGAE
SIDTVSAGS
>Mature_729_residues
MDDSTQAAEAVITTPTGARTVRFETGRLARQAAGAVVAHLGDTMVLSATTVSRSPKEQLDFFPLTVDVEERMYAAGRIPG
SFFRREGRPSEDAILTCRLIDRPLRPSFAKGLRNEIQVVATVLALDPDTLYDVVAINAASASTMLAGLPFSGPIGATRVG
YVDGDWIAFPTHSELERATFDMVVAGRVLEDGSDVAIMMVEAEATPGTVALVAGGAPAPTEEVVAAGLEAAKPAIRELCR
AQSELAAGAAKPQREFPLFIDYHDDVLETLTAAVGEELAAALRIAGKAERETELERVRALAVEKVSGQFEGRDKEISPAY
RALTKKLVRSRIVTDGVRIDGRSTTEIRELSAEVDYIPRVHGSALFERGETQILGVTTLAMLRMEQTVDTLNPDRTKRYM
HNYNFPPYSTGETGRVGSPKRREIGHGALAERALLPVLPSREEFPYAIRQVSEALSSNGSTSMGSVCASTLSLLNAGVPL
KAPVAGIAMGLIREGDAFVTLTDILGAEDAYGDMDFKVAGTQEFVTALQLDTKLDGIPASVLAGALQQARAARLTILEVM
AEAIGGPDEMSAHAPRVISVKIPVDKIGEVIGPKGKMINQIQADSGAEITVEDDGTIYIGAADGPAAETARSAINAIANP
QMPEVGERYLGTIVKITNFGAFVSLTPGKDGLLHVSKLKQLAGGKRVEKVEDVLSVGQKLQVEITEIDARGKISLAPGAE
SIDTVSAGS

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=723, Percent_Identity=36.3762102351314, Blast_Score=412, Evalue=1e-115,
Organism=Escherichia coli, GI145693187, Length=706, Percent_Identity=45.6090651558074, Blast_Score=583, Evalue=1e-167,
Organism=Caenorhabditis elegans, GI115534063, Length=670, Percent_Identity=32.8358208955224, Blast_Score=297, Evalue=1e-80,
Organism=Drosophila melanogaster, GI281362905, Length=726, Percent_Identity=36.0881542699724, Blast_Score=394, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24651641, Length=726, Percent_Identity=36.0881542699724, Blast_Score=394, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24651643, Length=726, Percent_Identity=36.0881542699724, Blast_Score=394, Evalue=1e-109,
Organism=Drosophila melanogaster, GI161079377, Length=657, Percent_Identity=36.2252663622527, Blast_Score=360, Evalue=3e-99,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR014069
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 77322; Mature: 77322

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDSTQAAEAVITTPTGARTVRFETGRLARQAAGAVVAHLGDTMVLSATTVSRSPKEQLD
CCCCCCCCCEEEECCCCCEEEEEEHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHCC
FFPLTVDVEERMYAAGRIPGSFFRREGRPSEDAILTCRLIDRPLRPSFAKGLRNEIQVVA
EEEEEEEHHHHHHHHCCCCHHHHHCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHH
TVLALDPDTLYDVVAINAASASTMLAGLPFSGPIGATRVGYVDGDWIAFPTHSELERATF
HHHHCCCCHHHHEEEECCCCHHHHEECCCCCCCCCCCEEEEECCCEEECCCHHHHHHHHH
DMVVAGRVLEDGSDVAIMMVEAEATPGTVALVAGGAPAPTEEVVAAGLEAAKPAIRELCR
HHHHHHHHCCCCCCEEEEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHCCHHHHHHHH
AQSELAAGAAKPQREFPLFIDYHDDVLETLTAAVGEELAAALRIAGKAERETELERVRAL
HHHHHHHCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
AVEKVSGQFEGRDKEISPAYRALTKKLVRSRIVTDGVRIDGRSTTEIRELSAEVDYIPRV
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHCCCC
HGSALFERGETQILGVTTLAMLRMEQTVDTLNPDRTKRYMHNYNFPPYSTGETGRVGSPK
CCHHHHHCCCCEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCCCH
RREIGHGALAERALLPVLPSREEFPYAIRQVSEALSSNGSTSMGSVCASTLSLLNAGVPL
HHHCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCC
KAPVAGIAMGLIREGDAFVTLTDILGAEDAYGDMDFKVAGTQEFVTALQLDTKLDGIPAS
CCCHHHHHHHHHCCCCCEEEEHHHHCCCCCCCCCCEEEECHHHHHHHHHHCCCCCCCCHH
VLAGALQQARAARLTILEVMAEAIGGPDEMSAHAPRVISVKIPVDKIGEVIGPKGKMINQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCHHHHHHHHCCCCHHHHH
IQADSGAEITVEDDGTIYIGAADGPAAETARSAINAIANPQMPEVGERYLGTIVKITNFG
HCCCCCCEEEEECCCEEEEECCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHEEECCC
AFVSLTPGKDGLLHVSKLKQLAGGKRVEKVEDVLSVGQKLQVEITEIDARGKISLAPGAE
EEEEECCCCCCCEEHHHHHHHHCCCHHHHHHHHHHCCCEEEEEEEEECCCCEEEECCCCC
SIDTVSAGS
CCCCCCCCC
>Mature Secondary Structure
MDDSTQAAEAVITTPTGARTVRFETGRLARQAAGAVVAHLGDTMVLSATTVSRSPKEQLD
CCCCCCCCCEEEECCCCCEEEEEEHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHCC
FFPLTVDVEERMYAAGRIPGSFFRREGRPSEDAILTCRLIDRPLRPSFAKGLRNEIQVVA
EEEEEEEHHHHHHHHCCCCHHHHHCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHH
TVLALDPDTLYDVVAINAASASTMLAGLPFSGPIGATRVGYVDGDWIAFPTHSELERATF
HHHHCCCCHHHHEEEECCCCHHHHEECCCCCCCCCCCEEEEECCCEEECCCHHHHHHHHH
DMVVAGRVLEDGSDVAIMMVEAEATPGTVALVAGGAPAPTEEVVAAGLEAAKPAIRELCR
HHHHHHHHCCCCCCEEEEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHCCHHHHHHHH
AQSELAAGAAKPQREFPLFIDYHDDVLETLTAAVGEELAAALRIAGKAERETELERVRAL
HHHHHHHCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
AVEKVSGQFEGRDKEISPAYRALTKKLVRSRIVTDGVRIDGRSTTEIRELSAEVDYIPRV
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHCCCC
HGSALFERGETQILGVTTLAMLRMEQTVDTLNPDRTKRYMHNYNFPPYSTGETGRVGSPK
CCHHHHHCCCCEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCCCH
RREIGHGALAERALLPVLPSREEFPYAIRQVSEALSSNGSTSMGSVCASTLSLLNAGVPL
HHHCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCC
KAPVAGIAMGLIREGDAFVTLTDILGAEDAYGDMDFKVAGTQEFVTALQLDTKLDGIPAS
CCCHHHHHHHHHCCCCCEEEEHHHHCCCCCCCCCCEEEECHHHHHHHHHHCCCCCCCCHH
VLAGALQQARAARLTILEVMAEAIGGPDEMSAHAPRVISVKIPVDKIGEVIGPKGKMINQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCHHHHHHHHCCCCHHHHH
IQADSGAEITVEDDGTIYIGAADGPAAETARSAINAIANPQMPEVGERYLGTIVKITNFG
HCCCCCCEEEEECCCEEEEECCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHEEECCC
AFVSLTPGKDGLLHVSKLKQLAGGKRVEKVEDVLSVGQKLQVEITEIDARGKISLAPGAE
EEEEECCCCCCCEEHHHHHHHHCCCHHHHHHHHHHCCCEEEEEEEEECCCCEEEECCCCC
SIDTVSAGS
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA