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The map label for this gene is leuD

Identifier: 158312966

GI number: 158312966

Start: 1332209

End: 1332796

Strand: Direct

Name: leuD

Synonym: Franean1_1114

Alternate gene names: 158312966

Gene position: 1332209-1332796 (Clockwise)

Preceding gene: 158312965

Following gene: 158312967

Centisome position: 14.83

GC content: 72.45

Gene sequence:

>588_bases
ATGGAGGCGTTCACCATCCACACCGGTCGGGCGGTGCCGCTGCGGCGCAGCGACGTCGACACCGACCAGATCATCCCGAG
CGAATGGCTCAAGCGGATCGAGCGGACGGGCTTCGGTGCCGGGCTGTTCTCCGAATGGCGGGCGGACCCGTCGTTCGTGC
TGAACGACCCCGCCCACGCCGGTGCGTCGATCCTGCTCGCCGGCCCGGACTTCGGCACCGGCTCGTCGCGTGAGCACGCG
GTCTGGGCGCTGCAGGACTACGGCTTCCGCGCGGTGCTCTCGCCCCGCTTCGCCGACATCTTCCGCGGCAACGCGCTGGG
CAACGGCCTGCTCCCGGTGGTGCTGCCGGCCGACACGGTCGAGGCGCTGACCGCGGCGGTGGAGGCCGACCCGACCACGG
AGATCACCGTCGACCTGGTGGCCCGGGAGGTCCGCGGCGCCGGTCAGGTCGCCGGCTTCGAGCTCGACGACTTCACCCGC
TGGCGGCTGATGGAGGGCCTGGACGACGTCGGCCTGACGCTGCGCCACGAGCAGGACATCACCGTGTTCGAGGCCAGCCG
GCCCGGGTGGCTCCCGACCACGGCCTGA

Upstream 100 bases:

>100_bases
GGCGCCCCGGCCCGGGTCGAACTCCGGGCTCCCGGACCCCGGGCCACCGGACTCCCAGACTTCCGAAACTTCCGAACGGA
CTCGAAGGGAACGTGCCGTC

Downstream 100 bases:

>100_bases
GCCTCCCGCGTCATCTGAGGATTTTCCCCGGGCCGAGCTGGGACGTCCCCGCTCGGCCCGGGATTTCCGGCCCACTGACG
GGCTCCCGGGCCGCCCTCGC

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 195; Mature: 195

Protein sequence:

>195_residues
MEAFTIHTGRAVPLRRSDVDTDQIIPSEWLKRIERTGFGAGLFSEWRADPSFVLNDPAHAGASILLAGPDFGTGSSREHA
VWALQDYGFRAVLSPRFADIFRGNALGNGLLPVVLPADTVEALTAAVEADPTTEITVDLVAREVRGAGQVAGFELDDFTR
WRLMEGLDDVGLTLRHEQDITVFEASRPGWLPTTA

Sequences:

>Translated_195_residues
MEAFTIHTGRAVPLRRSDVDTDQIIPSEWLKRIERTGFGAGLFSEWRADPSFVLNDPAHAGASILLAGPDFGTGSSREHA
VWALQDYGFRAVLSPRFADIFRGNALGNGLLPVVLPADTVEALTAAVEADPTTEITVDLVAREVRGAGQVAGFELDDFTR
WRLMEGLDDVGLTLRHEQDITVFEASRPGWLPTTA
>Mature_195_residues
MEAFTIHTGRAVPLRRSDVDTDQIIPSEWLKRIERTGFGAGLFSEWRADPSFVLNDPAHAGASILLAGPDFGTGSSREHA
VWALQDYGFRAVLSPRFADIFRGNALGNGLLPVVLPADTVEALTAAVEADPTTEITVDLVAREVRGAGQVAGFELDDFTR
WRLMEGLDDVGLTLRHEQDITVFEASRPGWLPTTA

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786258, Length=198, Percent_Identity=50, Blast_Score=199, Evalue=9e-53,
Organism=Saccharomyces cerevisiae, GI6321429, Length=200, Percent_Identity=38.5, Blast_Score=136, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEUD_FRASN (A8L568)

Other databases:

- EMBL:   CP000820
- RefSeq:   YP_001505474.1
- ProteinModelPortal:   A8L568
- SMR:   A8L568
- GeneID:   5669527
- GenomeReviews:   CP000820_GR
- KEGG:   fre:Franean1_1114
- HOGENOM:   HBG304838
- OMA:   TVDLTTC
- ProtClustDB:   PRK01641
- BioCyc:   FSP1855:FRANEAN1_1114-MONOMER
- HAMAP:   MF_01031
- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- Gene3D:   G3DSA:3.20.19.10
- PANTHER:   PTHR11670:SF2
- PANTHER:   PTHR11670
- TIGRFAMs:   TIGR00171

Pfam domain/function: PF00694 Aconitase_C; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.33

Molecular weight: Translated: 21230; Mature: 21230

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEAFTIHTGRAVPLRRSDVDTDQIIPSEWLKRIERTGFGAGLFSEWRADPSFVLNDPAHA
CCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHCCCCCEEECCCCCC
GASILLAGPDFGTGSSREHAVWALQDYGFRAVLSPRFADIFRGNALGNGLLPVVLPADTV
CCEEEEECCCCCCCCCCCCEEEEEECCCEEEEECCHHHHHHCCCCCCCCCEEEEECCHHH
EALTAAVEADPTTEITVDLVAREVRGAGQVAGFELDDFTRWRLMEGLDDVGLTLRHEQDI
HHHHHHHCCCCCCEEHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCE
TVFEASRPGWLPTTA
EEEECCCCCCCCCCC
>Mature Secondary Structure
MEAFTIHTGRAVPLRRSDVDTDQIIPSEWLKRIERTGFGAGLFSEWRADPSFVLNDPAHA
CCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHCCCCCEEECCCCCC
GASILLAGPDFGTGSSREHAVWALQDYGFRAVLSPRFADIFRGNALGNGLLPVVLPADTV
CCEEEEECCCCCCCCCCCCEEEEEECCCEEEEECCHHHHHHCCCCCCCCCEEEEECCHHH
EALTAAVEADPTTEITVDLVAREVRGAGQVAGFELDDFTRWRLMEGLDDVGLTLRHEQDI
HHHHHHHCCCCCCEEHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCE
TVFEASRPGWLPTTA
EEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA