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The map label for this gene is leuC

Identifier: 158312965

GI number: 158312965

Start: 1330711

End: 1332108

Strand: Direct

Name: leuC

Synonym: Franean1_1113

Alternate gene names: 158312965

Gene position: 1330711-1332108 (Clockwise)

Preceding gene: 158312962

Following gene: 158312966

Centisome position: 14.82

GC content: 73.39

Gene sequence:

>1398_bases
ATGGGTCGCACACTCGCGGAGAAGGTCTGGGACGCACACGTCGTCCGCCGCGCGGACGGAGAGCCCGACCTGCTCTACAT
CGATCTGCACCTCGTCCACGAGGTCACCTCGCCGCAGGCGTTCGAGGCGCTGCGGCTGGCCGGGCGCCCGGTCCGCCGTC
CCGACCTGACGCTGGCGACCGAGGACCACAACGTCCCGACGACCGACACGCTGCTGCCGATCGCCGACCCGGTCTCGCGG
GCGCAGGTCGAGGCGCTGCGCAAGAACTGCGCCGACTTCGGGGTCCGCCTGTTCCCGATGAACGACCCGGACCAGGGCAT
CGTCCACGTGGTCGGCCCGCAGCTCGGCCTGTCCGAGCCGGGCATGACGATCGTCTGTGGCGACAGCCACACCTCGACGC
ACGGCGCCTTCGGGGCGCTCGCCTTCGGCATCGGCACCAGCCAGGTCGAGCATGTGCTGGCCACCCAGACGCTGCCGCAG
CGCCGCCCGAAGACGATGGCGGTCACCGTCCAGGGTGAGCTGCCCGCCGGGGTCACCGCGAAGGACCTGATCCTCGCCGT
GATCGCCCGGATCGGCACGGGTGGCGGCGCCGGCTACGTCATCGAGTACCGCGGCGAAGCCGTCCGCGGGCTGTCGATGG
AGGGCCGGATGACGGTCTGCAACATGTCGATCGAGGCGGGCGCGCGCGCCGGGATGATCGCCCCGGACGAGACCACGTTC
GAGTACCTCAGGGGACGTCCGAACGCCCCGACCGGGGCCGACTGGGACGCCGCGGTCGAGTACTGGCGCACCCTGGCCAC
CGACCCCGACGCCACGTTCGACCACGAGGTCGTCATCGACGGGCCCAGCCTGAGCCCGTACGTCACCTGGGGGACCAACC
CGGGCCAGGCTGCGCCGCTGAGCTCGCCCGTGCCCGACCCGGCCGCCTATGCCGACCCGGCCGCGCGCGGCTCGGTGGAA
CGCGCCCTGGCCTACATGGATCTCGTGCCGGGCACCCCGCTGTCCGACGTCGCCGTCGACACCGTCTTCATCGGATCCTG
CACCAACGGCCGGATCTCCGACCTGCGTGACGCCGCCGACGTGCTGCGCGGGCGCCAGGTGGCGGACGGCCTTCGGGTCC
TGGTCGTCCCCGGCTCGATGGCGGTCAAGGCCGAGGCGGAGGCGGAGGGCCTCGACGAGGTGTTCCGCGCCGCGGGCGCC
GACTGGCGTAGCGCCGGCTGCTCGATGTGCCTGGGCATGAACCCGGACACCCTGCGGCCGGGGGAGCGCAGCGCGTCGAC
GTCCAACCGCAACTTCGAGGGCCGGCAGGGCCCCGGCGGGCGAACCCACCTCGTCTCGCCGGCGGTCGCCGCCGCCACCG
CGGTGACCGGCCGCCTCACGGCGCCCGCCGACCTGTAG

Upstream 100 bases:

>100_bases
CCGTGGGAGGCACGGCACGGCCATGGGGCGCGTGGTCCCGTCATGACAGCGAAGTCCGAGCAGCGTGGGCCCCGCCCACG
CGAGAGAGGGAGTGGGGCCA

Downstream 100 bases:

>100_bases
GGCGCCCCGGCCCGGGTCGAACTCCGGGCTCCCGGACCCCGGGCCACCGGACTCCCAGACTTCCGAAACTTCCGAACGGA
CTCGAAGGGAACGTGCCGTC

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MGRTLAEKVWDAHVVRRADGEPDLLYIDLHLVHEVTSPQAFEALRLAGRPVRRPDLTLATEDHNVPTTDTLLPIADPVSR
AQVEALRKNCADFGVRLFPMNDPDQGIVHVVGPQLGLSEPGMTIVCGDSHTSTHGAFGALAFGIGTSQVEHVLATQTLPQ
RRPKTMAVTVQGELPAGVTAKDLILAVIARIGTGGGAGYVIEYRGEAVRGLSMEGRMTVCNMSIEAGARAGMIAPDETTF
EYLRGRPNAPTGADWDAAVEYWRTLATDPDATFDHEVVIDGPSLSPYVTWGTNPGQAAPLSSPVPDPAAYADPAARGSVE
RALAYMDLVPGTPLSDVAVDTVFIGSCTNGRISDLRDAADVLRGRQVADGLRVLVVPGSMAVKAEAEAEGLDEVFRAAGA
DWRSAGCSMCLGMNPDTLRPGERSASTSNRNFEGRQGPGGRTHLVSPAVAAATAVTGRLTAPADL

Sequences:

>Translated_465_residues
MGRTLAEKVWDAHVVRRADGEPDLLYIDLHLVHEVTSPQAFEALRLAGRPVRRPDLTLATEDHNVPTTDTLLPIADPVSR
AQVEALRKNCADFGVRLFPMNDPDQGIVHVVGPQLGLSEPGMTIVCGDSHTSTHGAFGALAFGIGTSQVEHVLATQTLPQ
RRPKTMAVTVQGELPAGVTAKDLILAVIARIGTGGGAGYVIEYRGEAVRGLSMEGRMTVCNMSIEAGARAGMIAPDETTF
EYLRGRPNAPTGADWDAAVEYWRTLATDPDATFDHEVVIDGPSLSPYVTWGTNPGQAAPLSSPVPDPAAYADPAARGSVE
RALAYMDLVPGTPLSDVAVDTVFIGSCTNGRISDLRDAADVLRGRQVADGLRVLVVPGSMAVKAEAEAEGLDEVFRAAGA
DWRSAGCSMCLGMNPDTLRPGERSASTSNRNFEGRQGPGGRTHLVSPAVAAATAVTGRLTAPADL
>Mature_464_residues
GRTLAEKVWDAHVVRRADGEPDLLYIDLHLVHEVTSPQAFEALRLAGRPVRRPDLTLATEDHNVPTTDTLLPIADPVSRA
QVEALRKNCADFGVRLFPMNDPDQGIVHVVGPQLGLSEPGMTIVCGDSHTSTHGAFGALAFGIGTSQVEHVLATQTLPQR
RPKTMAVTVQGELPAGVTAKDLILAVIARIGTGGGAGYVIEYRGEAVRGLSMEGRMTVCNMSIEAGARAGMIAPDETTFE
YLRGRPNAPTGADWDAAVEYWRTLATDPDATFDHEVVIDGPSLSPYVTWGTNPGQAAPLSSPVPDPAAYADPAARGSVER
ALAYMDLVPGTPLSDVAVDTVFIGSCTNGRISDLRDAADVLRGRQVADGLRVLVVPGSMAVKAEAEAEGLDEVFRAAGAD
WRSAGCSMCLGMNPDTLRPGERSASTSNRNFEGRQGPGGRTHLVSPAVAAATAVTGRLTAPADL

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4501867, Length=380, Percent_Identity=28.1578947368421, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI8659555, Length=425, Percent_Identity=26.3529411764706, Blast_Score=94, Evalue=4e-19,
Organism=Escherichia coli, GI1786259, Length=459, Percent_Identity=63.8344226579521, Blast_Score=607, Evalue=1e-175,
Organism=Escherichia coli, GI1787531, Length=368, Percent_Identity=27.445652173913, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI87081781, Length=363, Percent_Identity=25.8953168044077, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI25149337, Length=380, Percent_Identity=29.4736842105263, Blast_Score=128, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI32564738, Length=380, Percent_Identity=29.4736842105263, Blast_Score=128, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI25149342, Length=308, Percent_Identity=30.1948051948052, Blast_Score=119, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI17568399, Length=403, Percent_Identity=26.0545905707196, Blast_Score=91, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6321429, Length=454, Percent_Identity=61.0132158590308, Blast_Score=566, Evalue=1e-162,
Organism=Saccharomyces cerevisiae, GI6320440, Length=488, Percent_Identity=27.0491803278689, Blast_Score=151, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6322261, Length=391, Percent_Identity=27.6214833759591, Blast_Score=139, Evalue=7e-34,
Organism=Saccharomyces cerevisiae, GI6323335, Length=366, Percent_Identity=29.5081967213115, Blast_Score=138, Evalue=2e-33,
Organism=Drosophila melanogaster, GI161076999, Length=395, Percent_Identity=27.0886075949367, Blast_Score=125, Evalue=8e-29,
Organism=Drosophila melanogaster, GI281365315, Length=394, Percent_Identity=26.9035532994924, Blast_Score=125, Evalue=9e-29,
Organism=Drosophila melanogaster, GI17864292, Length=394, Percent_Identity=26.9035532994924, Blast_Score=125, Evalue=9e-29,
Organism=Drosophila melanogaster, GI28571643, Length=401, Percent_Identity=26.9326683291771, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24645686, Length=368, Percent_Identity=25.8152173913043, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI17137564, Length=373, Percent_Identity=26.0053619302949, Blast_Score=89, Evalue=9e-18,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC_FRASN (A8L567)

Other databases:

- EMBL:   CP000820
- RefSeq:   YP_001505473.1
- ProteinModelPortal:   A8L567
- SMR:   A8L567
- GeneID:   5669526
- GenomeReviews:   CP000820_GR
- KEGG:   fre:Franean1_1113
- HOGENOM:   HBG330745
- OMA:   TLERNCA
- ProtClustDB:   PRK05478
- BioCyc:   FSP1855:FRANEAN1_1113-MONOMER
- HAMAP:   MF_01026
- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR00170

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33

Molecular weight: Translated: 48967; Mature: 48836

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRTLAEKVWDAHVVRRADGEPDLLYIDLHLVHEVTSPQAFEALRLAGRPVRRPDLTLAT
CCCHHHHHHHHHHHHHCCCCCCCEEEEEEEEEHHCCCCHHHHHHHHCCCCCCCCCCEEEE
EDHNVPTTDTLLPIADPVSRAQVEALRKNCADFGVRLFPMNDPDQGIVHVVGPQLGLSEP
CCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHCCEEEEECCCCCCCEEEEECCCCCCCCC
GMTIVCGDSHTSTHGAFGALAFGIGTSQVEHVLATQTLPQRRPKTMAVTVQGELPAGVTA
CCEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCH
KDLILAVIARIGTGGGAGYVIEYRGEAVRGLSMEGRMTVCNMSIEAGARAGMIAPDETTF
HHHHHHHHHHHCCCCCCEEEEEECCCEEECCCCCCCEEEEEEEHHCCCCCCCCCCCCHHH
EYLRGRPNAPTGADWDAAVEYWRTLATDPDATFDHEVVIDGPSLSPYVTWGTNPGQAAPL
HHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCEEEECCCCCCCCCC
SSPVPDPAAYADPAARGSVERALAYMDLVPGTPLSDVAVDTVFIGSCTNGRISDLRDAAD
CCCCCCCCCCCCCHHCCHHHHHHHHHHCCCCCCHHHHEEEEEEEECCCCCCHHHHHHHHH
VLRGRQVADGLRVLVVPGSMAVKAEAEAEGLDEVFRAAGADWRSAGCSMCLGMNPDTLRP
HHHCHHHCCCCEEEEECCCEEEEECCCCCCHHHHHHHCCCCHHHCCCCEEECCCCCCCCC
GERSASTSNRNFEGRQGPGGRTHLVSPAVAAATAVTGRLTAPADL
CCCCCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
GRTLAEKVWDAHVVRRADGEPDLLYIDLHLVHEVTSPQAFEALRLAGRPVRRPDLTLAT
CCHHHHHHHHHHHHHCCCCCCCEEEEEEEEEHHCCCCHHHHHHHHCCCCCCCCCCEEEE
EDHNVPTTDTLLPIADPVSRAQVEALRKNCADFGVRLFPMNDPDQGIVHVVGPQLGLSEP
CCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHCCEEEEECCCCCCCEEEEECCCCCCCCC
GMTIVCGDSHTSTHGAFGALAFGIGTSQVEHVLATQTLPQRRPKTMAVTVQGELPAGVTA
CCEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCH
KDLILAVIARIGTGGGAGYVIEYRGEAVRGLSMEGRMTVCNMSIEAGARAGMIAPDETTF
HHHHHHHHHHHCCCCCCEEEEEECCCEEECCCCCCCEEEEEEEHHCCCCCCCCCCCCHHH
EYLRGRPNAPTGADWDAAVEYWRTLATDPDATFDHEVVIDGPSLSPYVTWGTNPGQAAPL
HHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCEEEECCCCCCCCCC
SSPVPDPAAYADPAARGSVERALAYMDLVPGTPLSDVAVDTVFIGSCTNGRISDLRDAAD
CCCCCCCCCCCCCHHCCHHHHHHHHHHCCCCCCHHHHEEEEEEEECCCCCCHHHHHHHHH
VLRGRQVADGLRVLVVPGSMAVKAEAEAEGLDEVFRAAGADWRSAGCSMCLGMNPDTLRP
HHHCHHHCCCCEEEEECCCEEEEECCCCCCHHHHHHHCCCCHHHCCCCEEECCCCCCCCC
GERSASTSNRNFEGRQGPGGRTHLVSPAVAAATAVTGRLTAPADL
CCCCCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA