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The map label for this gene is entA [C]

Identifier: 158312438

GI number: 158312438

Start: 673508

End: 674332

Strand: Direct

Name: entA [C]

Synonym: Franean1_0581

Alternate gene names: 158312438

Gene position: 673508-674332 (Clockwise)

Preceding gene: 158312437

Following gene: 158312439

Centisome position: 7.5

GC content: 69.94

Gene sequence:

>825_bases
ATGACGCTGTTCGACGCGTTCCGATTCGACGGGAAGCGGGTCCTCGTGGTCGGCGGCGCGACCGGCATGGGTGCCGCCGC
CGCCGAGCTCGCCCAGGCAGCGGGCGCCGAGGTCATCGTCGCCGACTACGCCGAGGTCACCCTCCCCGGTGCGAAGTCGA
TCCACGTGAACCTCGCCGACGCCGCCTCCATCGACACAGCCGTCGACCAGCTCGGCGGACCCGTCCACGCCCTGCTCTCC
GCCGCCGGCGTCGCCGACGGCACCCCGGGCATCGAGCGGATCAACTTCATCGGCCACCGCTACCTCATCGACCGCCTGCT
GGCCGGCGGGCTGCTGCCCCGCGGCTCCGCGATCGGCTTCATCTCCTCCGCGGCCGGCCTCGGCTGGGAGGCCAACCTCC
CGCTACTCAAGGAATACCTGGCCATCACCGACTTCGACGAAGCCACCAAGTGGGCCGTCGAGCACGGCAAGGCCGACTAC
ATGTGGAGCAAGCAGGCCGTCTGCGCCTACGTCGCCACCCAGGCGATGCCCCTGCTCAAGCAGGGCATCCGCATCAACGC
GATCTGCCCCGGCCCCACCGACACCCCCCTCGCGCGGGCGAACGAGGAGAGGTGGCTCGGCTTCGGTACCGACTACCGGG
CCGAGCTGGGCATCGAGGCCGCGACCCCCCTGGAACAGGCCTACCCGCTGCTGTTCCTCTGCAGCGACGCCGCCTCCGCC
ATCAACGGCATCACCATCGTCACCGACTCGGGCTACTTCACCGCCGGCATCTCCGACTCCTTCCCCCCGGCCACACCCAT
CGTGAAATTCATGCTCGGTCGGTGA

Upstream 100 bases:

>100_bases
CTCACCGAGCTCGGACTCGACACCGACGCCGTCCTCGACCTCAAGATCCGCGGCGTCGTCGCATGACCCCCACCTCAACG
AAGAGCCAGGAGACATTGAG

Downstream 100 bases:

>100_bases
CCGGCTGCCTGGCGGCGCTCGCCGTCGGGCCCGTCCGTCCCTACTTCGGCACACCACCACGCAGCAGGCACTCGACATCC
ATTAAGGACGAAACCGATGA

Product: short-chain dehydrogenase/reductase SDR

Products: 2,3-Dihydroxybenzoate; H+; Nicotinamide adenine dinucleotide - reduced [C]

Alternate protein names: Short-Chain Dehydrogenase/Reductase SDR; NAD-Dependent Epimerase/Dehydratase; Dehydrogenase/Reductase; 3-Oxoacyl-(Acyl-Carrier-Protein) Reductase; Short-Chain Type Dehydrogenase/Reductase; Oxidoreductase Short-Chain Dehydrogenase/Reductase Family; Oxidoreductase Short Chain Dehydrogenase/Reductase Family

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MTLFDAFRFDGKRVLVVGGATGMGAAAAELAQAAGAEVIVADYAEVTLPGAKSIHVNLADAASIDTAVDQLGGPVHALLS
AAGVADGTPGIERINFIGHRYLIDRLLAGGLLPRGSAIGFISSAAGLGWEANLPLLKEYLAITDFDEATKWAVEHGKADY
MWSKQAVCAYVATQAMPLLKQGIRINAICPGPTDTPLARANEERWLGFGTDYRAELGIEAATPLEQAYPLLFLCSDAASA
INGITIVTDSGYFTAGISDSFPPATPIVKFMLGR

Sequences:

>Translated_274_residues
MTLFDAFRFDGKRVLVVGGATGMGAAAAELAQAAGAEVIVADYAEVTLPGAKSIHVNLADAASIDTAVDQLGGPVHALLS
AAGVADGTPGIERINFIGHRYLIDRLLAGGLLPRGSAIGFISSAAGLGWEANLPLLKEYLAITDFDEATKWAVEHGKADY
MWSKQAVCAYVATQAMPLLKQGIRINAICPGPTDTPLARANEERWLGFGTDYRAELGIEAATPLEQAYPLLFLCSDAASA
INGITIVTDSGYFTAGISDSFPPATPIVKFMLGR
>Mature_273_residues
TLFDAFRFDGKRVLVVGGATGMGAAAAELAQAAGAEVIVADYAEVTLPGAKSIHVNLADAASIDTAVDQLGGPVHALLSA
AGVADGTPGIERINFIGHRYLIDRLLAGGLLPRGSAIGFISSAAGLGWEANLPLLKEYLAITDFDEATKWAVEHGKADYM
WSKQAVCAYVATQAMPLLKQGIRINAICPGPTDTPLARANEERWLGFGTDYRAELGIEAATPLEQAYPLLFLCSDAASAI
NGITIVTDSGYFTAGISDSFPPATPIVKFMLGR

Specific function: Siderophore biosynthesis; enterobactin biosynthesis. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 1.3.1.28 [C]

Molecular weight: Translated: 28670; Mature: 28539

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLFDAFRFDGKRVLVVGGATGMGAAAAELAQAAGAEVIVADYAEVTLPGAKSIHVNLAD
CCCCCCEECCCCEEEEEECCCCCCHHHHHHHHHCCCEEEEECCCEEECCCCCEEEEEECC
AASIDTAVDQLGGPVHALLSAAGVADGTPGIERINFIGHRYLIDRLLAGGLLPRGSAIGF
HHHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEE
ISSAAGLGWEANLPLLKEYLAITDFDEATKWAVEHGKADYMWSKQAVCAYVATQAMPLLK
HHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCEECHHHHHHHHHHHHHHHHH
QGIRINAICPGPTDTPLARANEERWLGFGTDYRAELGIEAATPLEQAYPLLFLCSDAASA
CCCEEEEECCCCCCCCCCCCCCCCEEECCCCCHHHCCCCCCCCHHHHCCEEEEECCHHHH
INGITIVTDSGYFTAGISDSFPPATPIVKFMLGR
HCCEEEEECCCEEEECCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
TLFDAFRFDGKRVLVVGGATGMGAAAAELAQAAGAEVIVADYAEVTLPGAKSIHVNLAD
CCCCCEECCCCEEEEEECCCCCCHHHHHHHHHCCCEEEEECCCEEECCCCCEEEEEECC
AASIDTAVDQLGGPVHALLSAAGVADGTPGIERINFIGHRYLIDRLLAGGLLPRGSAIGF
HHHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEE
ISSAAGLGWEANLPLLKEYLAITDFDEATKWAVEHGKADYMWSKQAVCAYVATQAMPLLK
HHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCEECHHHHHHHHHHHHHHHHH
QGIRINAICPGPTDTPLARANEERWLGFGTDYRAELGIEAATPLEQAYPLLFLCSDAASA
CCCEEEEECCCCCCCCCCCCCCCCEEECCCCCHHHCCCCCCCCHHHHCCEEEEECCHHHH
INGITIVTDSGYFTAGISDSFPPATPIVKFMLGR
HCCEEEEECCCEEEECCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 5550 [C]

Specific activity: 66

Km value (mM): 0.26 {methyl-2,3-dehydro-3-hydroxybenzoate}} 0.2 {3-oxocyclohexane-1-carboxylic} 0.3 {2,3-dihydro-2,3-dihydroxybenzoate}} [C]

Substrates: 2,3-Dihydro-2,3-dihydroxybenzoate; Nicotinamide adenine dinucleotide [C]

Specific reaction: 2,3-Dihydro-2,3-dihydroxybenzoate + Nicotinamide adenine dinucleotide <==> 2,3-Dihydroxybenzoate + H+ + Nicotinamide adenine dinucleotide - reduced [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA