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The map label for this gene is glpK [H]

Identifier: 158312272

GI number: 158312272

Start: 486307

End: 487827

Strand: Direct

Name: glpK [H]

Synonym: Franean1_0408

Alternate gene names: 158312272

Gene position: 486307-487827 (Clockwise)

Preceding gene: 158312271

Following gene: 158312273

Centisome position: 5.41

GC content: 74.62

Gene sequence:

>1521_bases
GTGGCACCCGCGCGTGAGGGCCTCCTCCTGGCCATCGACCAGGGAACCAGCTCGACCAAGGCCGTGCTGGTCGACCGGGA
CGCGACGGTCGTCGCCTCGTCCTCCTGCCCAGTCGGACAGCGCCACCCAGGCCCCGGGCAGGTCGAGCAGAGCGCCGAGG
AGATCTGGGCCAGCGTCCAGCGGGCGGTCCGCACCTGCGTCGACCGGGAACTGGCCGCGCGCGTCGTGGGCGTCGCGATC
AGCGTCCAGCGCGAGTCCGTCGTCCTGTGGGACACCGTCTCCGGCGCCGCCGTGGGACCGATCCTCAGCTGGCAGGATCG
ACGCCTTGCCACCGCCACCGCCGCGGACGCCCTCGAGCGCGGCGGGCACGCGCCCTACATCCGGTCCTCTACCGGCCTGC
CCCTCGACCCGATGTTCTCCGCGCTCAAGGCCGGCTGGCTGCTCGACACCTACGACCCCAGCCGCGCCCGCGCCGGCACG
GGTGCCTGGAAGGTCGGCACCCTCGACGCCTGGCTGCTGTCCCGCTTCGGCGGCCCCGCCGTCACCGAGATCGGCAACGC
CTCCCGCACCCAGCTGCTCGACATCGGCGGCCGACAGTGGGATCCCACCCTCCTCGACCTGTTCGACGTCCCGCGCGCGG
CACTCCCCGACGTCGTCGCCTCACTCGGGCCGTTCCCTCCCGCGCGTGGCCTGCCGCCGCTGCCGGACGGGCTGCCCGTG
CTGGCGGTGCTCGGGGACTCGCACGCCGCGCTGTTCGCGCAGGCCAGGGGCGCTCCCGGCGTCGTGAAGGCCACCTACGG
CACCGGGTCGTCGGTCATGACCCTCGGCCGCGCCGACGCCGGCGACACCAGCCTGTGCTCCACCATCGCCTGGCAGCTCC
CCGGCACCGCCGGCACCGCCGCCGACGGCGTGGCGCACGCGACCGAGGCGAACATCCGCTCCACCGGCCGGACGATCAGC
TGGCTCGCCGACCTGTTCCAGGTCGACCCCACGGCTCTGCTCGCCGAGGCGGCTACGGCGAGCAGCGACGAGATCATCCT
AGTCCCCGCCTTCGGTGGCCTGGCCGCCCCGTGGTGGGATCGCGACGCGACCCCGGTGATCATCGGAGTCTCGCTGGGCA
CCGGCCGGCCGCAGCTCGCCCGCGCAGCCCTCGAATCCGTCGCCTTCCAGATCGACGACGTGATCACCGTGCTCCAGCGG
GCGGCCGGTCCGCTGGCCTTGCTGGCCTGCGACGGCGGACTGACCCGGTCCGAGGTGCTCATGCAGCTCCAGGCGGACGT
GAGCGGGCTGCCGGTCCGGATCTCCGCGACGCCGAACCTGTCCGCGCTGGGAGCGGCCCTGCTCGCCGGCGTGGGCGCCG
GCTGGTGGACCCTCGACGGCCTGCCCGAACCGCCCGGGCGAGCGACCGGCGCCGAGGTCCTTCCCCGGATCGACGCGGAC
ACTCGTGCTCGCCTCCGCAACCGCTGGGCCGACGCGGTGGCCCGCGCGCGTTCCCACCAGCGCACGAAGGCAGCGTCATG
A

Upstream 100 bases:

>100_bases
GTTCCTCGCCAGTTCGCCCCCACTGGGAGTACCCAGTTCCTGCTGGACCACTTCGGTCTCAACGCGGACGGCATCGTGGG
CGCGGCCCGAGAGGTGGTCG

Downstream 100 bases:

>100_bases
CCGGCGACGCGGCGTCCGCCGGCCGCGTCGCCGCGGACGGGCAGAACATACTGGTGCATGGCCATGCGCGCGAGCCCTGA
CCGCGATCGCCTGCGGCTGC

Product: carbohydrate kinase FGGY

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK [H]

Number of amino acids: Translated: 506; Mature: 505

Protein sequence:

>506_residues
MAPAREGLLLAIDQGTSSTKAVLVDRDATVVASSSCPVGQRHPGPGQVEQSAEEIWASVQRAVRTCVDRELAARVVGVAI
SVQRESVVLWDTVSGAAVGPILSWQDRRLATATAADALERGGHAPYIRSSTGLPLDPMFSALKAGWLLDTYDPSRARAGT
GAWKVGTLDAWLLSRFGGPAVTEIGNASRTQLLDIGGRQWDPTLLDLFDVPRAALPDVVASLGPFPPARGLPPLPDGLPV
LAVLGDSHAALFAQARGAPGVVKATYGTGSSVMTLGRADAGDTSLCSTIAWQLPGTAGTAADGVAHATEANIRSTGRTIS
WLADLFQVDPTALLAEAATASSDEIILVPAFGGLAAPWWDRDATPVIIGVSLGTGRPQLARAALESVAFQIDDVITVLQR
AAGPLALLACDGGLTRSEVLMQLQADVSGLPVRISATPNLSALGAALLAGVGAGWWTLDGLPEPPGRATGAEVLPRIDAD
TRARLRNRWADAVARARSHQRTKAAS

Sequences:

>Translated_506_residues
MAPAREGLLLAIDQGTSSTKAVLVDRDATVVASSSCPVGQRHPGPGQVEQSAEEIWASVQRAVRTCVDRELAARVVGVAI
SVQRESVVLWDTVSGAAVGPILSWQDRRLATATAADALERGGHAPYIRSSTGLPLDPMFSALKAGWLLDTYDPSRARAGT
GAWKVGTLDAWLLSRFGGPAVTEIGNASRTQLLDIGGRQWDPTLLDLFDVPRAALPDVVASLGPFPPARGLPPLPDGLPV
LAVLGDSHAALFAQARGAPGVVKATYGTGSSVMTLGRADAGDTSLCSTIAWQLPGTAGTAADGVAHATEANIRSTGRTIS
WLADLFQVDPTALLAEAATASSDEIILVPAFGGLAAPWWDRDATPVIIGVSLGTGRPQLARAALESVAFQIDDVITVLQR
AAGPLALLACDGGLTRSEVLMQLQADVSGLPVRISATPNLSALGAALLAGVGAGWWTLDGLPEPPGRATGAEVLPRIDAD
TRARLRNRWADAVARARSHQRTKAAS
>Mature_505_residues
APAREGLLLAIDQGTSSTKAVLVDRDATVVASSSCPVGQRHPGPGQVEQSAEEIWASVQRAVRTCVDRELAARVVGVAIS
VQRESVVLWDTVSGAAVGPILSWQDRRLATATAADALERGGHAPYIRSSTGLPLDPMFSALKAGWLLDTYDPSRARAGTG
AWKVGTLDAWLLSRFGGPAVTEIGNASRTQLLDIGGRQWDPTLLDLFDVPRAALPDVVASLGPFPPARGLPPLPDGLPVL
AVLGDSHAALFAQARGAPGVVKATYGTGSSVMTLGRADAGDTSLCSTIAWQLPGTAGTAADGVAHATEANIRSTGRTISW
LADLFQVDPTALLAEAATASSDEIILVPAFGGLAAPWWDRDATPVIIGVSLGTGRPQLARAALESVAFQIDDVITVLQRA
AGPLALLACDGGLTRSEVLMQLQADVSGLPVRISATPNLSALGAALLAGVGAGWWTLDGLPEPPGRATGAEVLPRIDADT
RARLRNRWADAVARARSHQRTKAAS

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism [H]

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family [H]

Homologues:

Organism=Homo sapiens, GI4504007, Length=512, Percent_Identity=32.421875, Blast_Score=228, Evalue=8e-60,
Organism=Homo sapiens, GI189409120, Length=512, Percent_Identity=32.421875, Blast_Score=228, Evalue=1e-59,
Organism=Homo sapiens, GI42794763, Length=520, Percent_Identity=32.3076923076923, Blast_Score=228, Evalue=1e-59,
Organism=Homo sapiens, GI41393575, Length=446, Percent_Identity=33.8565022421525, Blast_Score=221, Evalue=9e-58,
Organism=Homo sapiens, GI88196792, Length=521, Percent_Identity=26.679462571977, Blast_Score=159, Evalue=7e-39,
Organism=Escherichia coli, GI1790361, Length=500, Percent_Identity=35.2, Blast_Score=269, Evalue=3e-73,
Organism=Escherichia coli, GI1789168, Length=474, Percent_Identity=26.1603375527426, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1789987, Length=465, Percent_Identity=25.1612903225806, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI1789138, Length=502, Percent_Identity=23.3067729083665, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI1787789, Length=505, Percent_Identity=25.5445544554455, Blast_Score=69, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17535599, Length=509, Percent_Identity=30.4518664047151, Blast_Score=199, Evalue=3e-51,
Organism=Caenorhabditis elegans, GI71986140, Length=498, Percent_Identity=24.6987951807229, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI71986147, Length=234, Percent_Identity=27.7777777777778, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6321755, Length=484, Percent_Identity=28.099173553719, Blast_Score=149, Evalue=1e-36,
Organism=Drosophila melanogaster, GI24655555, Length=470, Percent_Identity=31.7021276595745, Blast_Score=213, Evalue=2e-55,
Organism=Drosophila melanogaster, GI24655560, Length=470, Percent_Identity=31.7021276595745, Blast_Score=213, Evalue=2e-55,
Organism=Drosophila melanogaster, GI24655570, Length=470, Percent_Identity=31.7021276595745, Blast_Score=213, Evalue=2e-55,
Organism=Drosophila melanogaster, GI24655574, Length=470, Percent_Identity=31.7021276595745, Blast_Score=213, Evalue=2e-55,
Organism=Drosophila melanogaster, GI24655565, Length=470, Percent_Identity=31.7021276595745, Blast_Score=213, Evalue=2e-55,
Organism=Drosophila melanogaster, GI17864214, Length=513, Percent_Identity=31.1890838206628, Blast_Score=209, Evalue=3e-54,
Organism=Drosophila melanogaster, GI24654573, Length=443, Percent_Identity=32.5056433408578, Blast_Score=201, Evalue=8e-52,
Organism=Drosophila melanogaster, GI19922058, Length=546, Percent_Identity=27.4725274725275, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24652871, Length=501, Percent_Identity=27.9441117764471, Blast_Score=151, Evalue=8e-37,
Organism=Drosophila melanogaster, GI24656367, Length=519, Percent_Identity=25.2408477842004, Blast_Score=139, Evalue=3e-33,
Organism=Drosophila melanogaster, GI21357867, Length=534, Percent_Identity=24.1573033707865, Blast_Score=135, Evalue=7e-32,
Organism=Drosophila melanogaster, GI24656372, Length=351, Percent_Identity=25.6410256410256, Blast_Score=105, Evalue=1e-22,
Organism=Drosophila melanogaster, GI45552909, Length=312, Percent_Identity=23.7179487179487, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999 [H]

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N [H]

EC number: =2.7.1.30 [H]

Molecular weight: Translated: 52409; Mature: 52278

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPAREGLLLAIDQGTSSTKAVLVDRDATVVASSSCPVGQRHPGPGQVEQSAEEIWASVQ
CCCCCCCEEEEEECCCCCCEEEEEECCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHH
RAVRTCVDRELAARVVGVAISVQRESVVLWDTVSGAAVGPILSWQDRRLATATAADALER
HHHHHHHHHHHHHHHHHHEEEEECCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHH
GGHAPYIRSSTGLPLDPMFSALKAGWLLDTYDPSRARAGTGAWKVGTLDAWLLSRFGGPA
CCCCCEEECCCCCCHHHHHHHHHCCCEEECCCCHHCCCCCCCEEECCHHHHHHHHCCCCH
VTEIGNASRTQLLDIGGRQWDPTLLDLFDVPRAALPDVVASLGPFPPARGLPPLPDGLPV
HHHCCCCCHHEEEECCCCCCCCHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCE
LAVLGDSHAALFAQARGAPGVVKATYGTGSSVMTLGRADAGDTSLCSTIAWQLPGTAGTA
EEEECCCCCEEHHHCCCCCCEEEEECCCCCCEEEECCCCCCCHHHHHHHHHCCCCCCCCC
ADGVAHATEANIRSTGRTISWLADLFQVDPTALLAEAATASSDEIILVPAFGGLAAPWWD
CCCHHHHHHCCHHHCCCHHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCCC
RDATPVIIGVSLGTGRPQLARAALESVAFQIDDVITVLQRAAGPLALLACDGGLTRSEVL
CCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHH
MQLQADVSGLPVRISATPNLSALGAALLAGVGAGWWTLDGLPEPPGRATGAEVLPRIDAD
HHHHCCCCCCCEEEECCCCHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCHHHHCCCCCH
TRARLRNRWADAVARARSHQRTKAAS
HHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
APAREGLLLAIDQGTSSTKAVLVDRDATVVASSSCPVGQRHPGPGQVEQSAEEIWASVQ
CCCCCCEEEEEECCCCCCEEEEEECCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHH
RAVRTCVDRELAARVVGVAISVQRESVVLWDTVSGAAVGPILSWQDRRLATATAADALER
HHHHHHHHHHHHHHHHHHEEEEECCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHH
GGHAPYIRSSTGLPLDPMFSALKAGWLLDTYDPSRARAGTGAWKVGTLDAWLLSRFGGPA
CCCCCEEECCCCCCHHHHHHHHHCCCEEECCCCHHCCCCCCCEEECCHHHHHHHHCCCCH
VTEIGNASRTQLLDIGGRQWDPTLLDLFDVPRAALPDVVASLGPFPPARGLPPLPDGLPV
HHHCCCCCHHEEEECCCCCCCCHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCE
LAVLGDSHAALFAQARGAPGVVKATYGTGSSVMTLGRADAGDTSLCSTIAWQLPGTAGTA
EEEECCCCCEEHHHCCCCCCEEEEECCCCCCEEEECCCCCCCHHHHHHHHHCCCCCCCCC
ADGVAHATEANIRSTGRTISWLADLFQVDPTALLAEAATASSDEIILVPAFGGLAAPWWD
CCCHHHHHHCCHHHCCCHHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCCC
RDATPVIIGVSLGTGRPQLARAALESVAFQIDDVITVLQRAAGPLALLACDGGLTRSEVL
CCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHH
MQLQADVSGLPVRISATPNLSALGAALLAGVGAGWWTLDGLPEPPGRATGAEVLPRIDAD
HHHHCCCCCCCEEEECCCCHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCHHHHCCCCCH
TRARLRNRWADAVARARSHQRTKAAS
HHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9162046; 12663927; 3011747 [H]