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The map label for this gene is rihB [H]

Identifier: 157964064

GI number: 157964064

Start: 5166414

End: 5167382

Strand: Direct

Name: rihB [H]

Synonym: Spea_4253

Alternate gene names: 157964064

Gene position: 5166414-5167382 (Clockwise)

Preceding gene: 157964044

Following gene: 157964066

Centisome position: 99.84

GC content: 45.1

Gene sequence:

>969_bases
ATGACAAATAAAATAATTCTCGATTCTGATCCCGGGATTGACGATGTAATGGCGATTCTGTTTGCTGAAGCGCACCCTGC
AATTGAGCTGAAAGCTATTACAACAGTCTACGGTAACGCGACTATCGAGAACGGTACTCGTAATGCTCTATTTTTAAAAC
AGAAGTATGGCTTTAATGCCGATGTGGTTAAGGGGGCTGATAAGCCTATCGTTCGCGACCCTGTTGGACCTACTATCGTG
GTTCATGGGGAAACTGGCCTAGGTGATGTACATGCGCCTACAGATCTAACTCTGTCTGCGGATCCTCGTCCTGCTTATCA
ATATATTATCGATTCTGTTAAGGCTGAACCGGGTGAGATCACCTTGGTTGCCGTAGGTCCTTTAACTAACTTGGCTTTAG
CACTGGAAGCTGCACCTGAGATAGCTGGCTTAGTAAAAGAAGTTGTTGTAATGGGCGGCGCGTTTGGTATGAACGATCAC
CGTGGTAACGTGACTCCTTATGCTGAGGCTAACATTCATGATGATCCGCATGCGGCAGATATCGTTTTCACTGCACCTTG
GCCTGTGGTTGTTATTGGCTTAGACGTGACAGAAGAGAGCTTCTTTAGTAAAGACTATCTTGATCAGCTAAGAGATGACG
CCGCTGAAGTTGGCCAGTTTATCTGGGACATTAGCCGCTATTATCTTAAGTTTTACTCCGAAAAAGTTGGCTTAGAAGGC
TGCCACGTACACGATCCGTCGGCAATTGCTTATGTTATCGATCCTTCTCTGTTTACCTTACGTGAAGGGCCTATTCGCGT
GGTAACAGATGGTCCAGCCGAAGGATTAACAATTCAGAAGTTTGATGAGCGTAAGTACCAACATGATGACTGGGGAATGA
ATCCGGCTCAAAAAGTTGGGGTAGGGGTAAGAGATGAAGCGCTATTAAAGCTTTATCGTGAAACGATTATTGAATACGGT
AATCGTTAA

Upstream 100 bases:

>100_bases
TATTTGGCTTAAAATCTATATCACGTTAATCAATGGCTTATAGAGGTATCTTACTGTCCACCTACACTAGATAGACTGTA
ATAATTAGGATTCAAATAAA

Downstream 100 bases:

>100_bases
TAAATTTATGAAAGTAAAAGCGGGGATAATTCCCCGCTTTTTTATTGGCTTTTTTGGTATTAACTGATGTTTTGCACTTG
AGAAGCGAGCAACCAAATAG

Product: inosine/uridine-preferring nucleoside hydrolase

Products: NA

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MTNKIILDSDPGIDDVMAILFAEAHPAIELKAITTVYGNATIENGTRNALFLKQKYGFNADVVKGADKPIVRDPVGPTIV
VHGETGLGDVHAPTDLTLSADPRPAYQYIIDSVKAEPGEITLVAVGPLTNLALALEAAPEIAGLVKEVVVMGGAFGMNDH
RGNVTPYAEANIHDDPHAADIVFTAPWPVVVIGLDVTEESFFSKDYLDQLRDDAAEVGQFIWDISRYYLKFYSEKVGLEG
CHVHDPSAIAYVIDPSLFTLREGPIRVVTDGPAEGLTIQKFDERKYQHDDWGMNPAQKVGVGVRDEALLKLYRETIIEYG
NR

Sequences:

>Translated_322_residues
MTNKIILDSDPGIDDVMAILFAEAHPAIELKAITTVYGNATIENGTRNALFLKQKYGFNADVVKGADKPIVRDPVGPTIV
VHGETGLGDVHAPTDLTLSADPRPAYQYIIDSVKAEPGEITLVAVGPLTNLALALEAAPEIAGLVKEVVVMGGAFGMNDH
RGNVTPYAEANIHDDPHAADIVFTAPWPVVVIGLDVTEESFFSKDYLDQLRDDAAEVGQFIWDISRYYLKFYSEKVGLEG
CHVHDPSAIAYVIDPSLFTLREGPIRVVTDGPAEGLTIQKFDERKYQHDDWGMNPAQKVGVGVRDEALLKLYRETIIEYG
NR
>Mature_321_residues
TNKIILDSDPGIDDVMAILFAEAHPAIELKAITTVYGNATIENGTRNALFLKQKYGFNADVVKGADKPIVRDPVGPTIVV
HGETGLGDVHAPTDLTLSADPRPAYQYIIDSVKAEPGEITLVAVGPLTNLALALEAAPEIAGLVKEVVVMGGAFGMNDHR
GNVTPYAEANIHDDPHAADIVFTAPWPVVVIGLDVTEESFFSKDYLDQLRDDAAEVGQFIWDISRYYLKFYSEKVGLEGC
HVHDPSAIAYVIDPSLFTLREGPIRVVTDGPAEGLTIQKFDERKYQHDDWGMNPAQKVGVGVRDEALLKLYRETIIEYGN
R

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively. Has a clear preference for cytidine over uridine. Strictly specific for ribonucleosides [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788486, Length=321, Percent_Identity=39.8753894080997, Blast_Score=196, Evalue=1e-51,
Organism=Escherichia coli, GI1786871, Length=281, Percent_Identity=37.0106761565836, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1786213, Length=275, Percent_Identity=38.9090909090909, Blast_Score=152, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI32565474, Length=274, Percent_Identity=32.4817518248175, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17565698, Length=190, Percent_Identity=34.7368421052632, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6320608, Length=268, Percent_Identity=31.7164179104478, Blast_Score=103, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24641839, Length=192, Percent_Identity=31.7708333333333, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24641837, Length=233, Percent_Identity=31.3304721030043, Blast_Score=72, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022977 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: =3.2.2.8 [H]

Molecular weight: Translated: 35172; Mature: 35041

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNKIILDSDPGIDDVMAILFAEAHPAIELKAITTVYGNATIENGTRNALFLKQKYGFNA
CCCCEEECCCCCHHHHHHHHHHCCCCCEEEEEEEEEECCCEECCCCCCEEEEEECCCCCC
DVVKGADKPIVRDPVGPTIVVHGETGLGDVHAPTDLTLSADPRPAYQYIIDSVKAEPGEI
CEECCCCCCCEECCCCCEEEEECCCCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCCCCE
TLVAVGPLTNLALALEAAPEIAGLVKEVVVMGGAFGMNDHRGNVTPYAEANIHDDPHAAD
EEEEECCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCCCCCCCCCE
IVFTAPWPVVVIGLDVTEESFFSKDYLDQLRDDAAEVGQFIWDISRYYLKFYSEKVGLEG
EEEECCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
CHVHDPSAIAYVIDPSLFTLREGPIRVVTDGPAEGLTIQKFDERKYQHDDWGMNPAQKVG
CEECCCCEEEEEECCCEEEECCCCEEEEECCCCCCCEEEEHHHHCCCCCCCCCCHHHHHC
VGVRDEALLKLYRETIIEYGNR
CCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TNKIILDSDPGIDDVMAILFAEAHPAIELKAITTVYGNATIENGTRNALFLKQKYGFNA
CCCEEECCCCCHHHHHHHHHHCCCCCEEEEEEEEEECCCEECCCCCCEEEEEECCCCCC
DVVKGADKPIVRDPVGPTIVVHGETGLGDVHAPTDLTLSADPRPAYQYIIDSVKAEPGEI
CEECCCCCCCEECCCCCEEEEECCCCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCCCCE
TLVAVGPLTNLALALEAAPEIAGLVKEVVVMGGAFGMNDHRGNVTPYAEANIHDDPHAAD
EEEEECCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCCCCCCCCCE
IVFTAPWPVVVIGLDVTEESFFSKDYLDQLRDDAAEVGQFIWDISRYYLKFYSEKVGLEG
EEEECCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
CHVHDPSAIAYVIDPSLFTLREGPIRVVTDGPAEGLTIQKFDERKYQHDDWGMNPAQKVG
CEECCCCEEEEEECCCEEEECCCCEEEEECCCCCCCEEEEHHHHCCCCCCCCCCHHHHHC
VGVRDEALLKLYRETIIEYGNR
CCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA