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The map label for this gene is fdoI [H]

Identifier: 157963938

GI number: 157963938

Start: 5012969

End: 5013952

Strand: Reverse

Name: fdoI [H]

Synonym: Spea_4127

Alternate gene names: 157963938

Gene position: 5013952-5012969 (Counterclockwise)

Preceding gene: 157963939

Following gene: 157963937

Centisome position: 96.9

GC content: 44.82

Gene sequence:

>984_bases
ATGAACAAATGGTTAAAACACATAGGCGTGGCAATTGCACTGTTTTTCAGTGTGTCTGTTTTTGCTAACGCTACTGACAA
AGTGGGCGACACTATTGAAGTTAACAACGGCCAAATCTGGGCTCAGCTAAAAGCGGGCGAAGAAGGTCGTACAACTTCAA
CGAGTGAATTCCACGGTCAAGCAATCAACACCTATGATCTACGTGTGCTTGAATTGCGTAGTGATATTCTTGCTCCAGCG
TTAATGGCAGCCCTTTTCGGCATGATCATTATTTTTGTTCTGTTTATCAAAGTGAATGGCATTTCTAAGCTTCACGGTGG
TTATTCAGGCAAGCTGGTATACCGTTGGTCTAAGTTTGACGTATCAATTCACTGGCTAGGTGCGATCCCTTGTCTGCTAT
TGATCCTAACAGGCTTAATGCTAATTGCTGGACGTTACTTTGTTCAGCCGTACATCGGTGAAGGCCTATGGGCTGGCCTA
GTTAACGCTGCTAAGCAGATCCACGACATCATGGCGATCCCGTTCATGTTAGGTTGGGCGCTTATGGTAACGTTATGGGC
TAAGAACCAGATGCCTAAAATGTATGACCTTAAGTGGATGATGGTTGTTGGTGGTTACATCAACTTCGGTCCATTCAAAG
GTAAGCACCCTGACGCTGGTTTTGCTAACGCGGGTGAGAAGTTATGGTTCTGGGCATTCGCTGTGTTCGGTCTAGTGATT
TCTGCATCAGGTATGTTGTTGCTATTCCCGAACTTGTTCGAGCCTAGCCGCACATTAAGCCTAATCGCATTAGTGCTTCA
CTCTGTAGCTGCTATCATCATTACTGCTTTCTCAATCGTGCATATCTTCATGGCAACGGTTATGTCTGAAGGTGGTATGG
AGTGTATGGTTTCTGGCTACTGTGACGAAACATGGGCAAGCCAACACCATAACCTTTGGTTCGATGAAATCAAAGAAAAC
GGTACCCTTAAGTACAAAGAATAA

Upstream 100 bases:

>100_bases
AAAACGCAGCTTGGGGTTAATCAACTTAGGTTGACTAACTTCCAGCGTACCCTGAGTGGCTCATTAGTTTGAGCCACCTA
GTGGTAATAGAGGACGTATT

Downstream 100 bases:

>100_bases
TCTTCTTTACACTTAATGGCTAAACACCTCAGCTAGTAATAGCTGGGGTGTTTTTATATCTGCTGCAAAATACAGCCTGT
ATGTTTTATGCGCAATCGCT

Product: formate dehydrogenase subunit gamma

Products: CO2; NADH

Alternate protein names: Aerobic formate dehydrogenase cytochrome b556 subunit; FDH-Z subunit gamma; Formate dehydrogenase-O subunit gamma [H]

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MNKWLKHIGVAIALFFSVSVFANATDKVGDTIEVNNGQIWAQLKAGEEGRTTSTSEFHGQAINTYDLRVLELRSDILAPA
LMAALFGMIIIFVLFIKVNGISKLHGGYSGKLVYRWSKFDVSIHWLGAIPCLLLILTGLMLIAGRYFVQPYIGEGLWAGL
VNAAKQIHDIMAIPFMLGWALMVTLWAKNQMPKMYDLKWMMVVGGYINFGPFKGKHPDAGFANAGEKLWFWAFAVFGLVI
SASGMLLLFPNLFEPSRTLSLIALVLHSVAAIIITAFSIVHIFMATVMSEGGMECMVSGYCDETWASQHHNLWFDEIKEN
GTLKYKE

Sequences:

>Translated_327_residues
MNKWLKHIGVAIALFFSVSVFANATDKVGDTIEVNNGQIWAQLKAGEEGRTTSTSEFHGQAINTYDLRVLELRSDILAPA
LMAALFGMIIIFVLFIKVNGISKLHGGYSGKLVYRWSKFDVSIHWLGAIPCLLLILTGLMLIAGRYFVQPYIGEGLWAGL
VNAAKQIHDIMAIPFMLGWALMVTLWAKNQMPKMYDLKWMMVVGGYINFGPFKGKHPDAGFANAGEKLWFWAFAVFGLVI
SASGMLLLFPNLFEPSRTLSLIALVLHSVAAIIITAFSIVHIFMATVMSEGGMECMVSGYCDETWASQHHNLWFDEIKEN
GTLKYKE
>Mature_327_residues
MNKWLKHIGVAIALFFSVSVFANATDKVGDTIEVNNGQIWAQLKAGEEGRTTSTSEFHGQAINTYDLRVLELRSDILAPA
LMAALFGMIIIFVLFIKVNGISKLHGGYSGKLVYRWSKFDVSIHWLGAIPCLLLILTGLMLIAGRYFVQPYIGEGLWAGL
VNAAKQIHDIMAIPFMLGWALMVTLWAKNQMPKMYDLKWMMVVGGYINFGPFKGKHPDAGFANAGEKLWFWAFAVFGLVI
SASGMLLLFPNLFEPSRTLSLIALVLHSVAAIIITAFSIVHIFMATVMSEGGMECMVSGYCDETWASQHHNLWFDEIKEN
GTLKYKE

Specific function: Allows to use formate as major electron donor during aerobic respiration. Subunit gamma is probably the cytochrome b556(FDO) component of the formate dehydrogenase [H]

COG id: COG2864

COG function: function code C; Cytochrome b subunit of formate dehydrogenase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the formate dehydrogenase gamma subunit family [H]

Homologues:

Organism=Escherichia coli, GI1790325, Length=109, Percent_Identity=33.9449541284404, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005797
- InterPro:   IPR016174
- InterPro:   IPR006471 [H]

Pfam domain/function: PF00033 Cytochrom_B_N [H]

EC number: 1.2.1.2

Molecular weight: Translated: 36382; Mature: 36382

Theoretical pI: Translated: 7.71; Mature: 7.71

Prosite motif: PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKWLKHIGVAIALFFSVSVFANATDKVGDTIEVNNGQIWAQLKAGEEGRTTSTSEFHGQ
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCEEEEEEECCCCCCCCCCHHHCCC
AINTYDLRVLELRSDILAPALMAALFGMIIIFVLFIKVNGISKLHGGYSGKLVYRWSKFD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCEEEEEEEEEE
VSIHWLGAIPCLLLILTGLMLIAGRYFVQPYIGEGLWAGLVNAAKQIHDIMAIPFMLGWA
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LMVTLWAKNQMPKMYDLKWMMVVGGYINFGPFKGKHPDAGFANAGEKLWFWAFAVFGLVI
HHHHHHHCCCCCCHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
SASGMLLLFPNLFEPSRTLSLIALVLHSVAAIIITAFSIVHIFMATVMSEGGMECMVSGY
HCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECC
CDETWASQHHNLWFDEIKENGTLKYKE
CCHHHHCCCCCCCHHHHCCCCCEEECC
>Mature Secondary Structure
MNKWLKHIGVAIALFFSVSVFANATDKVGDTIEVNNGQIWAQLKAGEEGRTTSTSEFHGQ
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCEEEEEEECCCCCCCCCCHHHCCC
AINTYDLRVLELRSDILAPALMAALFGMIIIFVLFIKVNGISKLHGGYSGKLVYRWSKFD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCEEEEEEEEEE
VSIHWLGAIPCLLLILTGLMLIAGRYFVQPYIGEGLWAGLVNAAKQIHDIMAIPFMLGWA
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LMVTLWAKNQMPKMYDLKWMMVVGGYINFGPFKGKHPDAGFANAGEKLWFWAFAVFGLVI
HHHHHHHCCCCCCHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
SASGMLLLFPNLFEPSRTLSLIALVLHSVAAIIITAFSIVHIFMATVMSEGGMECMVSGY
HCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECC
CDETWASQHHNLWFDEIKENGTLKYKE
CCHHHHCCCCCCCHHHHCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]