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The map label for this gene is hemN [H]

Identifier: 157963937

GI number: 157963937

Start: 5011375

End: 5012703

Strand: Reverse

Name: hemN [H]

Synonym: Spea_4126

Alternate gene names: 157963937

Gene position: 5012703-5011375 (Counterclockwise)

Preceding gene: 157963938

Following gene: 157963936

Centisome position: 96.87

GC content: 45.0

Gene sequence:

>1329_bases
ATGTCGTCAATTATTCTGCCTGATTCTGTTCAACCTTATAACGCCAATATCACCACGCCCGATTGGATGATTAGCGGTAT
GGAAAAAGTCATGCAGTTTTATGTGGACCGTAATTTACGACTCGATACGGTTTCGGCAGAGATGATGCCAAATCCAATTG
AAGGCAAAAAGTACATGCTGTATGCACATGTCCCTTTCTGCCATACCTTGTGCTCATTTTGTACTTTCCATCGTTTTCTG
TTTAAAGAAGATAAGGCGCGCGCTTATTTCATCTCCTTGCGTAAAGAGATGGATATGGTTAAAGCCTTAGGCTATGACTT
CGAGTCTATGTATATCGGTGGCGGTACCACAACCGTGCTTGAAGATGAGCTTGCACGCACTATCGAGCACGCTAAGAAGC
TATTCCCGAGCATTAAAGAAGTGTCTTGTGAGTCGGATCCACAGCACCTTGATAGTCCTGGCTTTAAGCAGCTTGAAGGC
TTAGTGGATCGTATGTCTATCGGCGTGCAGAGTTTCAATGACGATATTCTTGCCATGACAGATCGAATCGAGAAGTTCGG
TTCTGGACAACAGACTTTCGATAAGATCATGGCGGCAAAAGAGTTATTTCCGATCATCAACGTCGATCTTATTTTCGGTT
TCCGTGGCCAAACTGATGAAGTGATCCAACATGACTTGGACATGGCGTCAAAGCTAGATCCTCGCCAGATCACCACTTAT
CCATTGATGATCACTCACCAAACTCGTAAGAGCGTAAAAGGTAAGTTAGCTGCACCTCAGGGCGATATGGCGCGTCAATA
CCGCCAGATTCTGAATAGCTTAAACGGTCAGTACAATCAGTTATCAGCGTGGGCTTTTGGTAAAGCAAACGATGAGGGCT
TCGATGAGTACGTGATCGATTTTGATGAATACTTAGGGGTAGGTTCAGGCTCATTCAGTTTCTTGAATGACACGCTTTAT
GTGAATACCTTCTCGTTACGCAAGTACCAAGAGCGTATCGCTAATGGGCAGATGGGTGTTGACCAGCAGAAGAACTATGG
TCGTAAAGAAGTGATGCAGTATCGTTTCTTGCTAGGCATGTTCTCAGGTCGTCTATCGCGTAAGTATTTCCGTGAGACAT
TTGGCGTTAACCTAGACACAGCGCTGTTTAAAGAGATGACTTCGATGAAGGCGATTGGTGCGATTAAGAACGACCCAACT
GACCCTGATAACCTGATTGTTACCGACAACGGCAAGATGATGGGACTACTGATGATGAAAGAGTTCTATTCAGGCATGGA
TAACGTACGAGCGCAGTTACGTAAGCCACTAAAAGCCTGCGACATGTAG

Upstream 100 bases:

>100_bases
ATCACGTTTTATGCTTATTTAAAGTGCTAATTTTGTTCACCGCTAGTGCATTCCAATTTCCCGGTTCTTTTGTAATTAGC
ATTCTTCTATAGGAACTTAT

Downstream 100 bases:

>100_bases
CCTTGTAAACAAGTTAAAAAGGGTGACCGCGTTAGCGCCCACCCTTTTTTGCGATCAGTGCCTGTAGCTTTTTGATATCT
TCTTTGATCTGCAATACGTA

Product: coproporphyrinogen III oxidase

Products: NA

Alternate protein names: Coprogen oxidase; Coproporphyrinogenase [H]

Number of amino acids: Translated: 442; Mature: 441

Protein sequence:

>442_residues
MSSIILPDSVQPYNANITTPDWMISGMEKVMQFYVDRNLRLDTVSAEMMPNPIEGKKYMLYAHVPFCHTLCSFCTFHRFL
FKEDKARAYFISLRKEMDMVKALGYDFESMYIGGGTTTVLEDELARTIEHAKKLFPSIKEVSCESDPQHLDSPGFKQLEG
LVDRMSIGVQSFNDDILAMTDRIEKFGSGQQTFDKIMAAKELFPIINVDLIFGFRGQTDEVIQHDLDMASKLDPRQITTY
PLMITHQTRKSVKGKLAAPQGDMARQYRQILNSLNGQYNQLSAWAFGKANDEGFDEYVIDFDEYLGVGSGSFSFLNDTLY
VNTFSLRKYQERIANGQMGVDQQKNYGRKEVMQYRFLLGMFSGRLSRKYFRETFGVNLDTALFKEMTSMKAIGAIKNDPT
DPDNLIVTDNGKMMGLLMMKEFYSGMDNVRAQLRKPLKACDM

Sequences:

>Translated_442_residues
MSSIILPDSVQPYNANITTPDWMISGMEKVMQFYVDRNLRLDTVSAEMMPNPIEGKKYMLYAHVPFCHTLCSFCTFHRFL
FKEDKARAYFISLRKEMDMVKALGYDFESMYIGGGTTTVLEDELARTIEHAKKLFPSIKEVSCESDPQHLDSPGFKQLEG
LVDRMSIGVQSFNDDILAMTDRIEKFGSGQQTFDKIMAAKELFPIINVDLIFGFRGQTDEVIQHDLDMASKLDPRQITTY
PLMITHQTRKSVKGKLAAPQGDMARQYRQILNSLNGQYNQLSAWAFGKANDEGFDEYVIDFDEYLGVGSGSFSFLNDTLY
VNTFSLRKYQERIANGQMGVDQQKNYGRKEVMQYRFLLGMFSGRLSRKYFRETFGVNLDTALFKEMTSMKAIGAIKNDPT
DPDNLIVTDNGKMMGLLMMKEFYSGMDNVRAQLRKPLKACDM
>Mature_441_residues
SSIILPDSVQPYNANITTPDWMISGMEKVMQFYVDRNLRLDTVSAEMMPNPIEGKKYMLYAHVPFCHTLCSFCTFHRFLF
KEDKARAYFISLRKEMDMVKALGYDFESMYIGGGTTTVLEDELARTIEHAKKLFPSIKEVSCESDPQHLDSPGFKQLEGL
VDRMSIGVQSFNDDILAMTDRIEKFGSGQQTFDKIMAAKELFPIINVDLIFGFRGQTDEVIQHDLDMASKLDPRQITTYP
LMITHQTRKSVKGKLAAPQGDMARQYRQILNSLNGQYNQLSAWAFGKANDEGFDEYVIDFDEYLGVGSGSFSFLNDTLYV
NTFSLRKYQERIANGQMGVDQQKNYGRKEVMQYRFLLGMFSGRLSRKYFRETFGVNLDTALFKEMTSMKAIGAIKNDPTD
PDNLIVTDNGKMMGLLMMKEFYSGMDNVRAQLRKPLKACDM

Specific function: Anaerobic transformation of coproporphyrinogen-III into protoporphyrinogen-IX [H]

COG id: COG0635

COG function: function code H; Coproporphyrinogen III oxidase and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anaerobic coproporphyrinogen-III oxidase family [H]

Homologues:

Organism=Homo sapiens, GI8922911, Length=286, Percent_Identity=26.5734265734266, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI87082341, Length=309, Percent_Identity=26.8608414239482, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1789325, Length=263, Percent_Identity=26.615969581749, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR004559
- InterPro:   IPR010723
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06969 HemN_C; PF04055 Radical_SAM [H]

EC number: =1.3.99.22 [H]

Molecular weight: Translated: 50534; Mature: 50403

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
6.1 %Met     (Translated Protein)
7.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
5.9 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSIILPDSVQPYNANITTPDWMISGMEKVMQFYVDRNLRLDTVSAEMMPNPIEGKKYML
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEHHHCCCCCCCCEEEE
YAHVPFCHTLCSFCTFHRFLFKEDKARAYFISLRKEMDMVKALGYDFESMYIGGGTTTVL
EEECHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHEEECCCCHHHH
EDELARTIEHAKKLFPSIKEVSCESDPQHLDSPGFKQLEGLVDRMSIGVQSFNDDILAMT
HHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHH
DRIEKFGSGQQTFDKIMAAKELFPIINVDLIFGFRGQTDEVIQHDLDMASKLDPRQITTY
HHHHHHCCCHHHHHHHHHHHHHHHHEEEEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEE
PLMITHQTRKSVKGKLAAPQGDMARQYRQILNSLNGQYNQLSAWAFGKANDEGFDEYVID
EEEEEECHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHEEECCCCCCCCCHHHHHH
FDEYLGVGSGSFSFLNDTLYVNTFSLRKYQERIANGQMGVDQQKNYGRKEVMQYRFLLGM
HHHHHCCCCCCHHHHCCEEEEEEHHHHHHHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHH
FSGRLSRKYFRETFGVNLDTALFKEMTSMKAIGAIKNDPTDPDNLIVTDNGKMMGLLMMK
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCHHHHHHH
EFYSGMDNVRAQLRKPLKACDM
HHHCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SSIILPDSVQPYNANITTPDWMISGMEKVMQFYVDRNLRLDTVSAEMMPNPIEGKKYML
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEHHHCCCCCCCCEEEE
YAHVPFCHTLCSFCTFHRFLFKEDKARAYFISLRKEMDMVKALGYDFESMYIGGGTTTVL
EEECHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHEEECCCCHHHH
EDELARTIEHAKKLFPSIKEVSCESDPQHLDSPGFKQLEGLVDRMSIGVQSFNDDILAMT
HHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHH
DRIEKFGSGQQTFDKIMAAKELFPIINVDLIFGFRGQTDEVIQHDLDMASKLDPRQITTY
HHHHHHCCCHHHHHHHHHHHHHHHHEEEEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEE
PLMITHQTRKSVKGKLAAPQGDMARQYRQILNSLNGQYNQLSAWAFGKANDEGFDEYVID
EEEEEECHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHEEECCCCCCCCCHHHHHH
FDEYLGVGSGSFSFLNDTLYVNTFSLRKYQERIANGQMGVDQQKNYGRKEVMQYRFLLGM
HHHHHCCCCCCHHHHCCEEEEEEHHHHHHHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHH
FSGRLSRKYFRETFGVNLDTALFKEMTSMKAIGAIKNDPTDPDNLIVTDNGKMMGLLMMK
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCHHHHHHH
EFYSGMDNVRAQLRKPLKACDM
HHHCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8757728; 8969508; 9384377; 1339421 [H]