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The map label for this gene is atoC [H]

Identifier: 157963904

GI number: 157963904

Start: 4977275

End: 4978648

Strand: Reverse

Name: atoC [H]

Synonym: Spea_4093

Alternate gene names: 157963904

Gene position: 4978648-4977275 (Counterclockwise)

Preceding gene: 157963911

Following gene: 157963903

Centisome position: 96.21

GC content: 48.11

Gene sequence:

>1374_bases
ATGAATAATATGAAGCCACTTCCTTCAGCTGTATCTGTATTGATTGTCGATGATGAACCGGGCATGCGCAGTTTTTTGAA
AAAAGCGCTAGCGAAGAAATTTGCCTTGGTTGAAACCGCTTCGAGTGTCGAAGATGCTGAGCAATTGCGTTCACGCTGCC
ATTTTGATCTATTAATTGTCGATATTCGCCTACCTGGGCGCTCGGGAATTGAATGGCATGAGGCGTTAAATGATCAAGAA
AGGCGCTCTGACATCATCTTTATGACCGGCTACGCCGATATGGATGTGGCAATAAAGGCACTACGCGCTGGTGCATCTGA
TTTCATTATGAAGCCTTTCCACCTTGAACAGATGATGAAGGCGGTTGACCGCTGTATCGAGCGACGCTTGCTTAAGCGTG
AAAACTTGATGCTACGTCGTGAAGTGTCTTTCAATCAATCTTCTACCATTATTGGCCAAAGCGATGCGATGACTGAGGTG
AAGCATGTTATTGAACGTGTGGCGCCAACCAATGCCGTAGTCTTGATTGAGGGTGAGTCTGGTACGGGTAAAGAGTTGGT
TGCTCGTCAGTTGCATATTCTTAGTGGTCGCCAAGGACCATTTGTGCCAGTTAACTGTGGCGCCATCGCCCCAGAACTTT
TAGAGAGCGAGCTTTTCGGTCATTCTGCAGGTGCATTTACAGGCGCGAAGGGTAACCGAGAAGGTCTATTTAGTTTTGCC
TCTGGTGGTACAATCTTCCTCGATGAAATTGGCGAGATGCCGCTGAAAATGCAAACGGCGCTATTGCGCGTGCTTGAGCA
GCGTACCATTCGTCCCGTCGGTAGCGAGAAAGAGATCAATATCGATGTGCGCGTGTTGGCAGCGACTAACCGTAAGCTAT
CTGAAGAGGTTGAAGCGGGTAACTTTAGACGCGATCTATATTATCGCCTCAATGTGCTCGATATAGTGATCCCGCCGTTG
CGTGATCGCCCAGAAGATATCGTTGAGCTGACTCATCACTTCACTTGCCAATTAGCGGCAGAGCTAGGCGTGAAAGAGGT
GGTGTGGAGTCATGAAGATCTATTGAAGCTGCAACAACACGAATGGCCAGGTAATATTCGTGAGCTGCGAAATATGATTG
AACGCTGTATCCTATTGGGCAAGCCGCCTGCTGAATACTGGAAAGTGCAGCCAAAGACTGAGGCTGCAGGCGAGTCAGGT
TATCCACTACAGTGGGCGCTTAAAGATGTTGAGCGTGATCATGTGACTCGCGTTGTTGACCTACATGGTGGCAATAAGTC
GGCAGCAGCAAGGGATCTGGGGGTATCACGAAAGACTCTAGATCGAAAATATAAAGAATGGTTCGGCGGTGAAGCCGACA
AAGAAGAATCTTAA

Upstream 100 bases:

>100_bases
CGATTACATGGTTCATAGTGACCTATTTCGACTGGTTAGATGATAGACTTGCTCCATAATGAGTCAAAATGTCCCTCTAT
AAGACAAGGTGGGACAGAGA

Downstream 100 bases:

>100_bases
ACAATATTGAGATTGCTCTAGGGGAGTGTAGGTGTCTTTTTTTTCCAATATGTTTGGTGTTAGTTGGCAACAAATGCAGG
CTAAGGTGCGCTATCGCATC

Product: Fis family two component sigma54 specific transcriptional regulator

Products: NA

Alternate protein names: Ornithine decarboxylase antizyme; Ornithine/arginine decarboxylase inhibitor [H]

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MNNMKPLPSAVSVLIVDDEPGMRSFLKKALAKKFALVETASSVEDAEQLRSRCHFDLLIVDIRLPGRSGIEWHEALNDQE
RRSDIIFMTGYADMDVAIKALRAGASDFIMKPFHLEQMMKAVDRCIERRLLKRENLMLRREVSFNQSSTIIGQSDAMTEV
KHVIERVAPTNAVVLIEGESGTGKELVARQLHILSGRQGPFVPVNCGAIAPELLESELFGHSAGAFTGAKGNREGLFSFA
SGGTIFLDEIGEMPLKMQTALLRVLEQRTIRPVGSEKEINIDVRVLAATNRKLSEEVEAGNFRRDLYYRLNVLDIVIPPL
RDRPEDIVELTHHFTCQLAAELGVKEVVWSHEDLLKLQQHEWPGNIRELRNMIERCILLGKPPAEYWKVQPKTEAAGESG
YPLQWALKDVERDHVTRVVDLHGGNKSAAARDLGVSRKTLDRKYKEWFGGEADKEES

Sequences:

>Translated_457_residues
MNNMKPLPSAVSVLIVDDEPGMRSFLKKALAKKFALVETASSVEDAEQLRSRCHFDLLIVDIRLPGRSGIEWHEALNDQE
RRSDIIFMTGYADMDVAIKALRAGASDFIMKPFHLEQMMKAVDRCIERRLLKRENLMLRREVSFNQSSTIIGQSDAMTEV
KHVIERVAPTNAVVLIEGESGTGKELVARQLHILSGRQGPFVPVNCGAIAPELLESELFGHSAGAFTGAKGNREGLFSFA
SGGTIFLDEIGEMPLKMQTALLRVLEQRTIRPVGSEKEINIDVRVLAATNRKLSEEVEAGNFRRDLYYRLNVLDIVIPPL
RDRPEDIVELTHHFTCQLAAELGVKEVVWSHEDLLKLQQHEWPGNIRELRNMIERCILLGKPPAEYWKVQPKTEAAGESG
YPLQWALKDVERDHVTRVVDLHGGNKSAAARDLGVSRKTLDRKYKEWFGGEADKEES
>Mature_457_residues
MNNMKPLPSAVSVLIVDDEPGMRSFLKKALAKKFALVETASSVEDAEQLRSRCHFDLLIVDIRLPGRSGIEWHEALNDQE
RRSDIIFMTGYADMDVAIKALRAGASDFIMKPFHLEQMMKAVDRCIERRLLKRENLMLRREVSFNQSSTIIGQSDAMTEV
KHVIERVAPTNAVVLIEGESGTGKELVARQLHILSGRQGPFVPVNCGAIAPELLESELFGHSAGAFTGAKGNREGLFSFA
SGGTIFLDEIGEMPLKMQTALLRVLEQRTIRPVGSEKEINIDVRVLAATNRKLSEEVEAGNFRRDLYYRLNVLDIVIPPL
RDRPEDIVELTHHFTCQLAAELGVKEVVWSHEDLLKLQQHEWPGNIRELRNMIERCILLGKPPAEYWKVQPKTEAAGESG
YPLQWALKDVERDHVTRVVDLHGGNKSAAARDLGVSRKTLDRKYKEWFGGEADKEES

Specific function: Member of the two-component regulatory system AtoS/AtoC involved in the transcriptional regulation of the ato genes for acetoacetate metabolism. Also an inhibitor of polyamine biosynthesis [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=456, Percent_Identity=40.1315789473684, Blast_Score=323, Evalue=1e-89,
Organism=Escherichia coli, GI1790437, Length=438, Percent_Identity=39.9543378995434, Blast_Score=283, Evalue=2e-77,
Organism=Escherichia coli, GI1790299, Length=465, Percent_Identity=39.1397849462366, Blast_Score=281, Evalue=9e-77,
Organism=Escherichia coli, GI1788905, Length=427, Percent_Identity=35.8313817330211, Blast_Score=258, Evalue=4e-70,
Organism=Escherichia coli, GI1789233, Length=317, Percent_Identity=40.6940063091483, Blast_Score=234, Evalue=7e-63,
Organism=Escherichia coli, GI1786524, Length=322, Percent_Identity=43.167701863354, Blast_Score=234, Evalue=9e-63,
Organism=Escherichia coli, GI87082117, Length=253, Percent_Identity=49.4071146245059, Blast_Score=234, Evalue=1e-62,
Organism=Escherichia coli, GI1789087, Length=251, Percent_Identity=50.199203187251, Blast_Score=229, Evalue=3e-61,
Organism=Escherichia coli, GI87082152, Length=237, Percent_Identity=48.5232067510549, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI1787583, Length=324, Percent_Identity=37.037037037037, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI87081872, Length=231, Percent_Identity=42.8571428571429, Blast_Score=191, Evalue=9e-50,
Organism=Escherichia coli, GI1789828, Length=223, Percent_Identity=39.0134529147982, Blast_Score=143, Evalue=3e-35,
Organism=Escherichia coli, GI87081858, Length=294, Percent_Identity=30.6122448979592, Blast_Score=122, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 51396; Mature: 51396

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNMKPLPSAVSVLIVDDEPGMRSFLKKALAKKFALVETASSVEDAEQLRSRCHFDLLIV
CCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEE
DIRLPGRSGIEWHEALNDQERRSDIIFMTGYADMDVAIKALRAGASDFIMKPFHLEQMMK
EEECCCCCCCCHHHHCCCHHHCCCEEEEECCCCHHHHHHHHHCCCHHHHCCCHHHHHHHH
AVDRCIERRLLKRENLMLRREVSFNQSSTIIGQSDAMTEVKHVIERVAPTNAVVLIEGES
HHHHHHHHHHHHHHCHHEEEECCCCCCCEEECCCHHHHHHHHHHHHHCCCCEEEEEECCC
GTGKELVARQLHILSGRQGPFVPVNCGAIAPELLESELFGHSAGAFTGAKGNREGLFSFA
CCCHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEEC
SGGTIFLDEIGEMPLKMQTALLRVLEQRTIRPVGSEKEINIDVRVLAATNRKLSEEVEAG
CCCEEEEEHHCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEEECCCHHHHHHHCC
NFRRDLYYRLNVLDIVIPPLRDRPEDIVELTHHFTCQLAAELGVKEVVWSHEDLLKLQQH
CCHHHHHEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHCHHHHHHHHHC
EWPGNIRELRNMIERCILLGKPPAEYWKVQPKTEAAGESGYPLQWALKDVERDHVTRVVD
CCCCCHHHHHHHHHHHHHCCCCCHHHEECCCCCCCCCCCCCCHHHHHHHHHHHHHEEEEE
LHGGNKSAAARDLGVSRKTLDRKYKEWFGGEADKEES
ECCCCCCHHHHHCCCCHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MNNMKPLPSAVSVLIVDDEPGMRSFLKKALAKKFALVETASSVEDAEQLRSRCHFDLLIV
CCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEE
DIRLPGRSGIEWHEALNDQERRSDIIFMTGYADMDVAIKALRAGASDFIMKPFHLEQMMK
EEECCCCCCCCHHHHCCCHHHCCCEEEEECCCCHHHHHHHHHCCCHHHHCCCHHHHHHHH
AVDRCIERRLLKRENLMLRREVSFNQSSTIIGQSDAMTEVKHVIERVAPTNAVVLIEGES
HHHHHHHHHHHHHHCHHEEEECCCCCCCEEECCCHHHHHHHHHHHHHCCCCEEEEEECCC
GTGKELVARQLHILSGRQGPFVPVNCGAIAPELLESELFGHSAGAFTGAKGNREGLFSFA
CCCHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEEC
SGGTIFLDEIGEMPLKMQTALLRVLEQRTIRPVGSEKEINIDVRVLAATNRKLSEEVEAG
CCCEEEEEHHCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEEECCCHHHHHHHCC
NFRRDLYYRLNVLDIVIPPLRDRPEDIVELTHHFTCQLAAELGVKEVVWSHEDLLKLQQH
CCHHHHHEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHCHHHHHHHHHC
EWPGNIRELRNMIERCILLGKPPAEYWKVQPKTEAAGESGYPLQWALKDVERDHVTRVVD
CCCCCHHHHHHHHHHHHHCCCCCHHHEECCCCCCCCCCCCCCHHHHHHHHHHHHHEEEEE
LHGGNKSAAARDLGVSRKTLDRKYKEWFGGEADKEES
ECCCCCCHHHHHCCCCHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 8346225 [H]