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The map label for this gene is yfcE [H]

Identifier: 157963870

GI number: 157963870

Start: 4933159

End: 4933716

Strand: Direct

Name: yfcE [H]

Synonym: Spea_4059

Alternate gene names: 157963870

Gene position: 4933159-4933716 (Clockwise)

Preceding gene: 157963869

Following gene: 157963874

Centisome position: 95.33

GC content: 48.75

Gene sequence:

>558_bases
ATGAAACTTTTTATTGCCTCCGATCTACACGGCTCTGTTATAGCCACTCAAGCCATGCTGACTCGCTTTGAGCAGTCTGG
GGCTCAATACCTTATTCTGCTCGGAGACATGCTTAATCACGGCCCGCGTAACGCCATCCCCGACGGCTACAATCCCACGG
CCTTAGCTGAAACGCTGAACCAGTATGCCGATAAGATTATTGCCGTTCGCGGTAACTGCGACAGCGAAGTAGACCAAATG
CTACTGCAATTTCCGATCACCGCGAGCTTTGCTCAAGTATTGATGCCACAAATGCGTTTATTCCTCACCCACGGCCATAT
TTATAATCCCGATAACCTGCCACCGCTACAAGCTGGTGATGCACTAGTCTATGGTCATACCCACATTGCAGTGGCTGAAT
ATCGTGACGATATTTTATTGTTCAACCCGGGTTCAACCACCATTCCACGTAATGGTGCTCGAGCTTCTTACGGCTTAATC
ACTGACACAGAATGTCAGGTACGCGCCATAGACAACGATGAGCTACTACTCAGCTGTCCGATACGTCATACAAGATAG

Upstream 100 bases:

>100_bases
GTGAGCCTGCGAACGTCCTAGCTCCTAACCTATCTTTTACTAGCTTTATCGACAAACGAAGCTATATTGACGATCTGACT
CATCATTCTAGCCATAGTCC

Downstream 100 bases:

>100_bases
GTTCGAGGGTCTAGGGAATGCTAAGACGAGGACGGGCTTCGCCCTGCGAGGGCGTGACTGCGTCACTTCGAGAAAGGATT
AAAAGATAGGTCCTAGGTGC

Product: phosphodiesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 185; Mature: 185

Protein sequence:

>185_residues
MKLFIASDLHGSVIATQAMLTRFEQSGAQYLILLGDMLNHGPRNAIPDGYNPTALAETLNQYADKIIAVRGNCDSEVDQM
LLQFPITASFAQVLMPQMRLFLTHGHIYNPDNLPPLQAGDALVYGHTHIAVAEYRDDILLFNPGSTTIPRNGARASYGLI
TDTECQVRAIDNDELLLSCPIRHTR

Sequences:

>Translated_185_residues
MKLFIASDLHGSVIATQAMLTRFEQSGAQYLILLGDMLNHGPRNAIPDGYNPTALAETLNQYADKIIAVRGNCDSEVDQM
LLQFPITASFAQVLMPQMRLFLTHGHIYNPDNLPPLQAGDALVYGHTHIAVAEYRDDILLFNPGSTTIPRNGARASYGLI
TDTECQVRAIDNDELLLSCPIRHTR
>Mature_185_residues
MKLFIASDLHGSVIATQAMLTRFEQSGAQYLILLGDMLNHGPRNAIPDGYNPTALAETLNQYADKIIAVRGNCDSEVDQM
LLQFPITASFAQVLMPQMRLFLTHGHIYNPDNLPPLQAGDALVYGHTHIAVAEYRDDILLFNPGSTTIPRNGARASYGLI
TDTECQVRAIDNDELLLSCPIRHTR

Specific function: Shows phosphodiesterase activity [H]

COG id: COG0622

COG function: function code R; Predicted phosphoesterase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallophosphoesterase superfamily. YfcE family [H]

Homologues:

Organism=Escherichia coli, GI1788638, Length=181, Percent_Identity=58.0110497237569, Blast_Score=227, Evalue=3e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR020935
- InterPro:   IPR000979 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: NA

Molecular weight: Translated: 20435; Mature: 20435

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLFIASDLHGSVIATQAMLTRFEQSGAQYLILLGDMLNHGPRNAIPDGYNPTALAETLN
CEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEEEHHHCCCCCCCCCCCCCHHHHHHHHH
QYADKIIAVRGNCDSEVDQMLLQFPITASFAQVLMPQMRLFLTHGHIYNPDNLPPLQAGD
HHHHEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCC
ALVYGHTHIAVAEYRDDILLFNPGSTTIPRNGARASYGLITDTECQVRAIDNDELLLSCP
EEEECCEEEEEEECCCCEEEECCCCCCCCCCCCCCCCCCEECCCEEEEEECCCCEEEECC
IRHTR
CCCCC
>Mature Secondary Structure
MKLFIASDLHGSVIATQAMLTRFEQSGAQYLILLGDMLNHGPRNAIPDGYNPTALAETLN
CEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEEEHHHCCCCCCCCCCCCCHHHHHHHHH
QYADKIIAVRGNCDSEVDQMLLQFPITASFAQVLMPQMRLFLTHGHIYNPDNLPPLQAGD
HHHHEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCC
ALVYGHTHIAVAEYRDDILLFNPGSTTIPRNGARASYGLITDTECQVRAIDNDELLLSCP
EEEECCEEEEEEECCCCEEEECCCCCCCCCCCCCCCCCCEECCCEEEEEECCCCEEEECC
IRHTR
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]