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The map label for this gene is aes [H]

Identifier: 157963842

GI number: 157963842

Start: 4895880

End: 4896818

Strand: Reverse

Name: aes [H]

Synonym: Spea_4031

Alternate gene names: 157963842

Gene position: 4896818-4895880 (Counterclockwise)

Preceding gene: 157963843

Following gene: 157963841

Centisome position: 94.63

GC content: 47.82

Gene sequence:

>939_bases
ATGCCGTTAGATCCAAAAGTTGCGCTGTTTTTACAGCAAGTGCATGATTCTGGCGCACAAGCCTATGAAGATATGACGCC
GGCAGAGTCGAGGCGCTTAGATATGAATGAGCTGACTAAGATGAAAGGCGACCGAGTGTTAGAGCCCGTGGCCGCAATAG
AGCATAGTTTTATTCCTGGGCCGACGGCGGATCTGCCCATTAGGATCTACCGCCCGAAAGGTGACACGAGCGAGTTACAG
CCGGCAATTATTTTTATTCATGGCAGCGGCTGGGTGGTATCGAACATAGAGACTAATGATCATTTTAGCCGTGCCCTCGC
TAATCGTACCGGATCGGTTGTTATCGCAATTAATTATCAGAAGGCACCGGAACACAAGTTTCCTATCCCAATGGATGACT
GTTATGCGTCGACACTGTGGATATTTGAACATGCAAGTTTACTGGGGTTAGACTCGAACCGCATCGGTATATTTGGTGAT
AGTGCGGGTGGTAATCTTGCGGCGGCTGTGACCTTAAGATTACGTGATGAACAAGGACCAAAACTGGCATTTCAAGTATT
AGTTTATCCAGCGGTGCAATATGGCTGGCAGACGCCATCGGCCATTGCTCATGCAGAAGGCTATCTACTGCAACAGGCCA
GTATGAAGTATTATTGGGGCCACTACCTGCGTAGCGAAGCCGATGCAATAAATCCCTATTGTTCTCCGCTGAATGCTCAG
AGCCACAAAGATCTGCCACCAACACTGATCTACACTGCAGAGTTTGATCCACTATGCGATGACGGTTACCTCTATGCTCG
TGAACTGCAAGGCTCTGGCATCAAAGTGAAATACCGCTGTTTTGATGGCGTGATCCATGGATTTATTAAGATGCTTGGGG
TGTTCGAACAGGCCGATGAGTTTCTCGATGAACTTAAGCAAGATCTGTTAGAGATCTAG

Upstream 100 bases:

>100_bases
AGCGCCTCAGGCGGCACACCTAATAATTGAAACGATTAATTGAAACTATAGACAGTTGAACAGCTGTGGCTGGGAGACTG
TAATAGAGAAGGGAAGTAGT

Downstream 100 bases:

>100_bases
GTTTAATTTATTTTACATCACATCTGGAACTGCTCGCAAAACAAACAATTTCGTGAGCTTGTTCCAAATATTGTCATCTA
GTCGCCTAACTCAAATTAAT

Product: alpha/beta hydrolase domain-containing protein

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 312; Mature: 311

Protein sequence:

>312_residues
MPLDPKVALFLQQVHDSGAQAYEDMTPAESRRLDMNELTKMKGDRVLEPVAAIEHSFIPGPTADLPIRIYRPKGDTSELQ
PAIIFIHGSGWVVSNIETNDHFSRALANRTGSVVIAINYQKAPEHKFPIPMDDCYASTLWIFEHASLLGLDSNRIGIFGD
SAGGNLAAAVTLRLRDEQGPKLAFQVLVYPAVQYGWQTPSAIAHAEGYLLQQASMKYYWGHYLRSEADAINPYCSPLNAQ
SHKDLPPTLIYTAEFDPLCDDGYLYARELQGSGIKVKYRCFDGVIHGFIKMLGVFEQADEFLDELKQDLLEI

Sequences:

>Translated_312_residues
MPLDPKVALFLQQVHDSGAQAYEDMTPAESRRLDMNELTKMKGDRVLEPVAAIEHSFIPGPTADLPIRIYRPKGDTSELQ
PAIIFIHGSGWVVSNIETNDHFSRALANRTGSVVIAINYQKAPEHKFPIPMDDCYASTLWIFEHASLLGLDSNRIGIFGD
SAGGNLAAAVTLRLRDEQGPKLAFQVLVYPAVQYGWQTPSAIAHAEGYLLQQASMKYYWGHYLRSEADAINPYCSPLNAQ
SHKDLPPTLIYTAEFDPLCDDGYLYARELQGSGIKVKYRCFDGVIHGFIKMLGVFEQADEFLDELKQDLLEI
>Mature_311_residues
PLDPKVALFLQQVHDSGAQAYEDMTPAESRRLDMNELTKMKGDRVLEPVAAIEHSFIPGPTADLPIRIYRPKGDTSELQP
AIIFIHGSGWVVSNIETNDHFSRALANRTGSVVIAINYQKAPEHKFPIPMDDCYASTLWIFEHASLLGLDSNRIGIFGDS
AGGNLAAAVTLRLRDEQGPKLAFQVLVYPAVQYGWQTPSAIAHAEGYLLQQASMKYYWGHYLRSEADAINPYCSPLNAQS
HKDLPPTLIYTAEFDPLCDDGYLYARELQGSGIKVKYRCFDGVIHGFIKMLGVFEQADEFLDELKQDLLEI

Specific function: Displays esterase activity towards short chain fatty esters (acyl chain length of up to 8 carbons). Able to hydrolyze triacetylglycerol (triacetin) and tributyrylglycerol (tributyrin), but not trioleylglycerol (triolein) or cholesterol oleate. Negatively

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI68299767, Length=186, Percent_Identity=36.0215053763441, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI157041239, Length=138, Percent_Identity=36.231884057971, Blast_Score=105, Evalue=6e-23,
Organism=Homo sapiens, GI206597554, Length=137, Percent_Identity=37.2262773722628, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI61966717, Length=285, Percent_Identity=27.719298245614, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI68051721, Length=288, Percent_Identity=26.3888888888889, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI157041237, Length=107, Percent_Identity=37.3831775700935, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI21328446, Length=91, Percent_Identity=37.3626373626374, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI226423947, Length=296, Percent_Identity=25.6756756756757, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI1786682, Length=264, Percent_Identity=31.0606060606061, Blast_Score=122, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17540028, Length=314, Percent_Identity=25.4777070063694, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI71996133, Length=308, Percent_Identity=29.2207792207792, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17567059, Length=265, Percent_Identity=30.188679245283, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI72001146, Length=155, Percent_Identity=32.9032258064516, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI115533412, Length=137, Percent_Identity=27.7372262773723, Blast_Score=66, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI115533410, Length=137, Percent_Identity=27.7372262773723, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 34822; Mature: 34691

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLDPKVALFLQQVHDSGAQAYEDMTPAESRRLDMNELTKMKGDRVLEPVAAIEHSFIPG
CCCCHHHHHHHHHHHHCHHHHHHCCCCHHHHCCCHHHHHHHCCCHHHHHHHHHHHCCCCC
PTADLPIRIYRPKGDTSELQPAIIFIHGSGWVVSNIETNDHFSRALANRTGSVVIAINYQ
CCCCCCEEEECCCCCCCCCCEEEEEEECCCEEEEECCCCHHHHHHHHCCCCCEEEEEECC
KAPEHKFPIPMDDCYASTLWIFEHASLLGLDSNRIGIFGDSAGGNLAAAVTLRLRDEQGP
CCCCCCCCCCHHHHHHHHHHEECCHHEEECCCCCEEEEECCCCCCEEEEEEEEEECCCCC
KLAFQVLVYPAVQYGWQTPSAIAHAEGYLLQQASMKYYWGHYLRSEADAINPYCSPLNAQ
CEEEHHHHHHHHHHCCCCCHHHHHHCCHHHHHHCHHHHHHHHHHCCHHHCCCCCCCCCCC
SHKDLPPTLIYTAEFDPLCDDGYLYARELQGSGIKVKYRCFDGVIHGFIKMLGVFEQADE
CCCCCCCEEEEEECCCCCCCCCEEEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHH
FLDELKQDLLEI
HHHHHHHHHHCC
>Mature Secondary Structure 
PLDPKVALFLQQVHDSGAQAYEDMTPAESRRLDMNELTKMKGDRVLEPVAAIEHSFIPG
CCCHHHHHHHHHHHHCHHHHHHCCCCHHHHCCCHHHHHHHCCCHHHHHHHHHHHCCCCC
PTADLPIRIYRPKGDTSELQPAIIFIHGSGWVVSNIETNDHFSRALANRTGSVVIAINYQ
CCCCCCEEEECCCCCCCCCCEEEEEEECCCEEEEECCCCHHHHHHHHCCCCCEEEEEECC
KAPEHKFPIPMDDCYASTLWIFEHASLLGLDSNRIGIFGDSAGGNLAAAVTLRLRDEQGP
CCCCCCCCCCHHHHHHHHHHEECCHHEEECCCCCEEEEECCCCCCEEEEEEEEEECCCCC
KLAFQVLVYPAVQYGWQTPSAIAHAEGYLLQQASMKYYWGHYLRSEADAINPYCSPLNAQ
CEEEHHHHHHHHHHCCCCCHHHHHHCCHHHHHHCHHHHHHHHHHCCHHHCCCCCCCCCCC
SHKDLPPTLIYTAEFDPLCDDGYLYARELQGSGIKVKYRCFDGVIHGFIKMLGVFEQADE
CCCCCCCEEEEEECCCCCCCCCEEEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHH
FLDELKQDLLEI
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA