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The map label for this gene is mtr [H]

Identifier: 157963806

GI number: 157963806

Start: 4853380

End: 4854636

Strand: Reverse

Name: mtr [H]

Synonym: Spea_3995

Alternate gene names: 157963806

Gene position: 4854636-4853380 (Counterclockwise)

Preceding gene: 157963807

Following gene: 157963804

Centisome position: 93.82

GC content: 47.73

Gene sequence:

>1257_bases
ATGGCAAATCATATGAATGCAGCCAAGAACAAGGTTGCAGGTAAGTCGCTGTTAGGTGGGGCCATGATTATTGCGGGCAC
AGCTGTCGGCGCAGGCATGTTTTCTCTGCCTGTTGTAGGCGCTGGAATGTGGTTCGGTTACTCGCTATTGATGATGGCTG
TTGTGTGGCTTTGTATGTTGGTGTCGGGCCTAATGCTGCTTGAAACTAACCTGCACTTCGAGCCTGGAGCGAGCTTCGAC
ACCTTAACCAAAGCCACTCTAGGCCAGTTCTGGCGTATCGTAAATGGCCTATCGATTGCGTTTGTTCTGTATATTTTGAC
CTATGCCTATATCAGTGGTGGTGGTTCAATCGTAAATCATAGCCTTGAAGGCTTCGGGATTAACCTGCCTCAAAGCGTTG
CCGGATTGGTATTCGCTGGTGTGCTAGCAACTATCGTCATAATCAGTACTAAGGCGGTCGATAGAATTACCACTGTGATG
CTGGGTGGTATGATTATTACCTTCTTCCTCGCTGTGGGTAATCTGTTACTCGACGTCGAGTCGGTGAAGCTGTTGAGCCC
TGATGGTGAAAACAAGTATCTGCCTTATATGCTAGCGGCGCTGCCTTTTGGTCTAGTGAGCTTTGGCTATCATGGCAATG
TGCCCAGCCTAGTTAAATATTACGGTAAAGATCACCGCACCATTATCAAGGCGATAGTGATTGGTACTGGTATCGCATTG
GTTATCTATGTCTGCTGGCTTGTTGCAACCATGGGTAATATCTCTAGAACTGGTTTTATTGGTATCATAGAGCAGGGCGG
CAATATGGGCGTGCTAGTGAACGCTCTGTCAGGCGTGATAACCAGTGATTGGTTAACGACTATGCTTACTCTGTTTGCTA
ACCTCGCCGTAGCCTCATCTTTCTTGGGAGTGACTTTAGGTCTATTTGATTATCTGGCGGATCTATTTGGATTTGATGAG
TCGCGCTCGGGTCGACTAAAGACTGCAGCGGTGACCTTCTTACCTCCAACATTACTTGGCCTATTATTTCCAAATGGCTT
CCTCATAGCTATTGGTTTTGCGGCGCTGGCAGCCACTATCTGGGCAGCAATTGTTCCGGCAATGATGGCTTATAAGGTCA
GGGCTATGTATCCAGATAGTACCAGCTATAAAGTACCTGGTGGCAACGCGGTGATCGCCATAGTGATCCTGTTTGGTGTG
ATTACTGCTGCATGTCACTTATTGGCAATGGCGGATATGCTGCCGCTATACGGTTAG

Upstream 100 bases:

>100_bases
GCAGCGATATCAAACGTATTAAAGAGAGAGCGAATCTACTGTTACGCTCGTCCGTAAGGCAAACGATTGTAGATAGTGTG
CATGCGGAGCAGAATACTTA

Downstream 100 bases:

>100_bases
TCGTTAGCTGCGGGCCAATCAAAAACTAAAGCCTCTTTTAAAGAGGCTTTTTGCTATTTAGATTTAGCTTATGCAAATAT
TGAGTCGGTGTTTGACGACG

Product: aromatic amino acid transporter

Products: Indole [Cytoplasm]; L-tryptophan [Cytoplasm]; Proton [Cytoplasm] [C]

Alternate protein names: Tryptophan permease [H]

Number of amino acids: Translated: 418; Mature: 417

Protein sequence:

>418_residues
MANHMNAAKNKVAGKSLLGGAMIIAGTAVGAGMFSLPVVGAGMWFGYSLLMMAVVWLCMLVSGLMLLETNLHFEPGASFD
TLTKATLGQFWRIVNGLSIAFVLYILTYAYISGGGSIVNHSLEGFGINLPQSVAGLVFAGVLATIVIISTKAVDRITTVM
LGGMIITFFLAVGNLLLDVESVKLLSPDGENKYLPYMLAALPFGLVSFGYHGNVPSLVKYYGKDHRTIIKAIVIGTGIAL
VIYVCWLVATMGNISRTGFIGIIEQGGNMGVLVNALSGVITSDWLTTMLTLFANLAVASSFLGVTLGLFDYLADLFGFDE
SRSGRLKTAAVTFLPPTLLGLLFPNGFLIAIGFAALAATIWAAIVPAMMAYKVRAMYPDSTSYKVPGGNAVIAIVILFGV
ITAACHLLAMADMLPLYG

Sequences:

>Translated_418_residues
MANHMNAAKNKVAGKSLLGGAMIIAGTAVGAGMFSLPVVGAGMWFGYSLLMMAVVWLCMLVSGLMLLETNLHFEPGASFD
TLTKATLGQFWRIVNGLSIAFVLYILTYAYISGGGSIVNHSLEGFGINLPQSVAGLVFAGVLATIVIISTKAVDRITTVM
LGGMIITFFLAVGNLLLDVESVKLLSPDGENKYLPYMLAALPFGLVSFGYHGNVPSLVKYYGKDHRTIIKAIVIGTGIAL
VIYVCWLVATMGNISRTGFIGIIEQGGNMGVLVNALSGVITSDWLTTMLTLFANLAVASSFLGVTLGLFDYLADLFGFDE
SRSGRLKTAAVTFLPPTLLGLLFPNGFLIAIGFAALAATIWAAIVPAMMAYKVRAMYPDSTSYKVPGGNAVIAIVILFGV
ITAACHLLAMADMLPLYG
>Mature_417_residues
ANHMNAAKNKVAGKSLLGGAMIIAGTAVGAGMFSLPVVGAGMWFGYSLLMMAVVWLCMLVSGLMLLETNLHFEPGASFDT
LTKATLGQFWRIVNGLSIAFVLYILTYAYISGGGSIVNHSLEGFGINLPQSVAGLVFAGVLATIVIISTKAVDRITTVML
GGMIITFFLAVGNLLLDVESVKLLSPDGENKYLPYMLAALPFGLVSFGYHGNVPSLVKYYGKDHRTIIKAIVIGTGIALV
IYVCWLVATMGNISRTGFIGIIEQGGNMGVLVNALSGVITSDWLTTMLTLFANLAVASSFLGVTLGLFDYLADLFGFDES
RSGRLKTAAVTFLPPTLLGLLFPNGFLIAIGFAALAATIWAAIVPAMMAYKVRAMYPDSTSYKVPGGNAVIAIVILFGVI
TAACHLLAMADMLPLYG

Specific function: Involved in transporting tryptophan across the cytoplasmic membrane [H]

COG id: COG0814

COG function: function code E; Amino acid permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid/polyamine transporter 2 family. Mtr/TnaB/TyrP permease subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789552, Length=386, Percent_Identity=58.0310880829015, Blast_Score=429, Evalue=1e-121,
Organism=Escherichia coli, GI1790145, Length=417, Percent_Identity=46.2829736211031, Blast_Score=350, Evalue=7e-98,
Organism=Escherichia coli, GI1788218, Length=411, Percent_Identity=36.0097323600973, Blast_Score=244, Evalue=1e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002091
- InterPro:   IPR013061
- InterPro:   IPR013059
- InterPro:   IPR018227 [H]

Pfam domain/function: PF03222 Trp_Tyr_perm [H]

EC number: NA

Molecular weight: Translated: 44086; Mature: 43955

Theoretical pI: Translated: 8.40; Mature: 8.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ANHMNAAKNKVAGKSLLGGAMIIAGTAVGAGMFSLPVVGAGMWFGYSLLMMAVVWLCML
CCCHHHHHHHHCCHHHHCCHHHEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSGLMLLETNLHFEPGASFDTLTKATLGQFWRIVNGLSIAFVLYILTYAYISGGGSIVNH
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHC
SLEGFGINLPQSVAGLVFAGVLATIVIISTKAVDRITTVMLGGMIITFFLAVGNLLLDVE
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SVKLLSPDGENKYLPYMLAALPFGLVSFGYHGNVPSLVKYYGKDHRTIIKAIVIGTGIAL
HHEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHH
VIYVCWLVATMGNISRTGFIGIIEQGGNMGVLVNALSGVITSDWLTTMLTLFANLAVASS
HHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLGVTLGLFDYLADLFGFDESRSGRLKTAAVTFLPPTLLGLLFPNGFLIAIGFAALAATI
HHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEHHCCHHHHHHHCCCCHHHHHHHHHHHHHH
WAAIVPAMMAYKVRAMYPDSTSYKVPGGNAVIAIVILFGVITAACHLLAMADMLPLYG
HHHHHHHHHHHHHHHCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Indole [Periplasm]; L-tryptophan [Periplasm]; Proton [Periplasm] [C]

Specific reaction: Proton [Periplasm] + indole [Periplasm] = Proton [Cytoplasm] + indole [Cytoplasm] L-tryptophan [Periplasm] + Proton [Periplasm] = L-tryptophan [Cytoplasm] + Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1987112; 2061290; 9278503; 1904443; 7814318 [H]