Click here to switch to the map view.

The map label for this gene is mdtL [H]

Identifier: 157963767

GI number: 157963767

Start: 4810554

End: 4811756

Strand: Reverse

Name: mdtL [H]

Synonym: Spea_3956

Alternate gene names: 157963767

Gene position: 4811756-4810554 (Counterclockwise)

Preceding gene: 157963770

Following gene: 157963765

Centisome position: 92.99

GC content: 50.29

Gene sequence:

>1203_bases
ATGCGTCGTAGTTTGTTACCCATTTTGTTGCCAATGGTGATTTTAAGCCCGTTGGCTATCGATATTTATCTCCCCTCTAT
GCCTACAATGGCAGCGGAGTTTGCTGTATCAGCTAGCGAAATCCAATCGACGTTAGTACTCTTCTTGTTTGCCATGGGCA
CTGGCCAGTTATTGATTGGTCCACTGGCGGATCGCTATGGTCGCCGACCTATTGCCATAGGTGGCATCATCTTTTATATG
ATTAGCAGTCTATTGGCCGCTATTGCAGTAGAGTTTCATTGGCTGCAAATTGCTCGAGTGATGCAAGGCTTAGCCGCTTG
TTCCACCTCTATCGTGGTATTCAGTGCGGTGCGGGACTGTTTTTCACCCAAAGAGAGCGCTCGTTACTACAGCTATCTTA
ACGGTATGATCTGTATTATTCCCGCGTTAGCACCGACCTTGGGCGGCATGCTGGCGCTGCAATTTGGCTGGCGTTCAAAC
TTCATCTTTATGGCCTTGTATGGCCTAGTTATTCTGGCTTTAGTGAGCCTGCGTCTACCTGAAACTCGCCCACAGAATAC
CATCAGTGACGGGCCACTATACCGCTGGGCGCGTTATAAGCCGGTGATTACTGAGCCGCACTTTGTGTTTTATGCCATCG
CTTGTATGGCGGGTATGGCTGCAATCCTGAGTTATGTGTCTTACGCACCTGTGTGGCTTATCGGTCACTTGGGTGTATCA
GAGTTAACTTTCAGTGGCCTATTTGGCTTAAACGCGGCCGTGAATATCGTTGCCTGTTTTGCCGCACCACTAGTGATTAA
AAAGCTGGGCAACCGACCAACCGTTATTGCTGCGTTAATCTTGATGCTAGCGTCTGCAGTCACTCAGGTTATTGTGCAGT
TGGTGGGTCCGCAGTCAGGCTTGATGGCAGCGTATAGCTACATGTTACCTATGATGCTGCTCTGTATCGGCTTTGCCTTA
CTACTCGGCCCAGCAACCAGTATGGCGCTTTCGGCCTTTGGCGAAAGAGCGGGTACGGCAACAGCAATGCTTGGTTTCAT
TCAGATGAGTGGCGCCTCACTGCTGACGGCACTTATCCAGCAGACGAGTCTGCCTGCTCCGTATGCGGTAGCGGCCTTGA
TGGGCGTGGGAGCGATTGCATTACTTGCCATGATGGCTATGCCTAAGTTTGACCATTGGCACCAAGAGCAACACGCTTAC
TAG

Upstream 100 bases:

>100_bases
AGATATTCCTCAGTGGAAATTCACAATGGAAATAATATCATTTCTGTTCATACCATAGTTTGATTACTATAGCGCTGTTT
TCCTAATTCTGAGAAAGATC

Downstream 100 bases:

>100_bases
TTTGTGTTGGGCAAGTGCCCGATAGTAAACAGTAACAGTTAACTTTGTTTCACCCTGAAACACTTTAGCCTGCACAGACT
AACCATCTGGGCAGGCTTTT

Product: Bcr/CflA subfamily drug resistance transporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MRRSLLPILLPMVILSPLAIDIYLPSMPTMAAEFAVSASEIQSTLVLFLFAMGTGQLLIGPLADRYGRRPIAIGGIIFYM
ISSLLAAIAVEFHWLQIARVMQGLAACSTSIVVFSAVRDCFSPKESARYYSYLNGMICIIPALAPTLGGMLALQFGWRSN
FIFMALYGLVILALVSLRLPETRPQNTISDGPLYRWARYKPVITEPHFVFYAIACMAGMAAILSYVSYAPVWLIGHLGVS
ELTFSGLFGLNAAVNIVACFAAPLVIKKLGNRPTVIAALILMLASAVTQVIVQLVGPQSGLMAAYSYMLPMMLLCIGFAL
LLGPATSMALSAFGERAGTATAMLGFIQMSGASLLTALIQQTSLPAPYAVAALMGVGAIALLAMMAMPKFDHWHQEQHAY

Sequences:

>Translated_400_residues
MRRSLLPILLPMVILSPLAIDIYLPSMPTMAAEFAVSASEIQSTLVLFLFAMGTGQLLIGPLADRYGRRPIAIGGIIFYM
ISSLLAAIAVEFHWLQIARVMQGLAACSTSIVVFSAVRDCFSPKESARYYSYLNGMICIIPALAPTLGGMLALQFGWRSN
FIFMALYGLVILALVSLRLPETRPQNTISDGPLYRWARYKPVITEPHFVFYAIACMAGMAAILSYVSYAPVWLIGHLGVS
ELTFSGLFGLNAAVNIVACFAAPLVIKKLGNRPTVIAALILMLASAVTQVIVQLVGPQSGLMAAYSYMLPMMLLCIGFAL
LLGPATSMALSAFGERAGTATAMLGFIQMSGASLLTALIQQTSLPAPYAVAALMGVGAIALLAMMAMPKFDHWHQEQHAY
>Mature_400_residues
MRRSLLPILLPMVILSPLAIDIYLPSMPTMAAEFAVSASEIQSTLVLFLFAMGTGQLLIGPLADRYGRRPIAIGGIIFYM
ISSLLAAIAVEFHWLQIARVMQGLAACSTSIVVFSAVRDCFSPKESARYYSYLNGMICIIPALAPTLGGMLALQFGWRSN
FIFMALYGLVILALVSLRLPETRPQNTISDGPLYRWARYKPVITEPHFVFYAIACMAGMAAILSYVSYAPVWLIGHLGVS
ELTFSGLFGLNAAVNIVACFAAPLVIKKLGNRPTVIAALILMLASAVTQVIVQLVGPQSGLMAAYSYMLPMMLLCIGFAL
LLGPATSMALSAFGERAGTATAMLGFIQMSGASLLTALIQQTSLPAPYAVAALMGVGAIALLAMMAMPKFDHWHQEQHAY

Specific function: Unknown

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. DHA1 family. MdtL (TC 2.A.1.2.22) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790146, Length=344, Percent_Identity=31.6860465116279, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI87082312, Length=349, Percent_Identity=29.2263610315186, Blast_Score=114, Evalue=9e-27,
Organism=Escherichia coli, GI1788509, Length=373, Percent_Identity=26.2734584450402, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI48994889, Length=239, Percent_Identity=29.2887029288703, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1787065, Length=334, Percent_Identity=24.251497005988, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790794, Length=230, Percent_Identity=23.9130434782609, Blast_Score=74, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6319657, Length=168, Percent_Identity=30.3571428571429, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324264, Length=189, Percent_Identity=24.8677248677249, Blast_Score=67, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6322071, Length=170, Percent_Identity=27.0588235294118, Blast_Score=63, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42989; Mature: 42989

Theoretical pI: Translated: 8.91; Mature: 8.91

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
6.0 %Met     (Translated Protein)
7.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
6.0 %Met     (Mature Protein)
7.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRSLLPILLPMVILSPLAIDIYLPSMPTMAAEFAVSASEIQSTLVLFLFAMGTGQLLIG
CCCCHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
PLADRYGRRPIAIGGIIFYMISSLLAAIAVEFHWLQIARVMQGLAACSTSIVVFSAVRDC
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FSPKESARYYSYLNGMICIIPALAPTLGGMLALQFGWRSNFIFMALYGLVILALVSLRLP
HCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHCCC
ETRPQNTISDGPLYRWARYKPVITEPHFVFYAIACMAGMAAILSYVSYAPVWLIGHLGVS
CCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
ELTFSGLFGLNAAVNIVACFAAPLVIKKLGNRPTVIAALILMLASAVTQVIVQLVGPQSG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHH
LMAAYSYMLPMMLLCIGFALLLGPATSMALSAFGERAGTATAMLGFIQMSGASLLTALIQ
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
QTSLPAPYAVAALMGVGAIALLAMMAMPKFDHWHQEQHAY
HCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCC
>Mature Secondary Structure
MRRSLLPILLPMVILSPLAIDIYLPSMPTMAAEFAVSASEIQSTLVLFLFAMGTGQLLIG
CCCCHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
PLADRYGRRPIAIGGIIFYMISSLLAAIAVEFHWLQIARVMQGLAACSTSIVVFSAVRDC
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FSPKESARYYSYLNGMICIIPALAPTLGGMLALQFGWRSNFIFMALYGLVILALVSLRLP
HCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHCCC
ETRPQNTISDGPLYRWARYKPVITEPHFVFYAIACMAGMAAILSYVSYAPVWLIGHLGVS
CCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
ELTFSGLFGLNAAVNIVACFAAPLVIKKLGNRPTVIAALILMLASAVTQVIVQLVGPQSG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHH
LMAAYSYMLPMMLLCIGFALLLGPATSMALSAFGERAGTATAMLGFIQMSGASLLTALIQ
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
QTSLPAPYAVAALMGVGAIALLAMMAMPKFDHWHQEQHAY
HCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]