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The map label for this gene is glnG [H]

Identifier: 157963741

GI number: 157963741

Start: 4778165

End: 4779574

Strand: Direct

Name: glnG [H]

Synonym: Spea_3930

Alternate gene names: 157963741

Gene position: 4778165-4779574 (Clockwise)

Preceding gene: 157963740

Following gene: 157963744

Centisome position: 92.34

GC content: 52.27

Gene sequence:

>1410_bases
ATGACAGAACAAGTTTGGATCCTCGACGACGATAGTTCGATACGTTGGGTACTAGAAAAAGCACTACAGAGCGCCAAATT
TAGCAGCGCAAGCTTTGCCGCAGCGGAATCCTTATGGCAAGCCTTAGAGACGGCGCAGCCTCAGGTGATAGTGTCAGACA
TTCGTATGCCTGGCACCGATGGTTTAACGCTATTAGAGCGCCTGCAAAACCATTACCCGCATATTCCGGTTATCATCATG
ACGGCGCATTCCGATCTCGACAGCGCGGTAAGTGCCTATCAAGCGGGCGCCTTTGAGTATCTGCCTAAGCCTTTCGATAT
CGATGAAGCGATATCCTTAGTCGACCGCGCCCTGACTCACGCCAAAGAGCAGTCTTCGACCATCACGACCGAAGAGCCGA
TAGTCGCCACGCCAGAGATCATCGGCGAAGCCCCTGCCATGCAGGAGGTCTTTAGGGCCATTGGCCGCCTTTCTCGCTCA
TCCATTAGTGTATTGATTAATGGCCAATCTGGTACGGGTAAAGAGCTGGTTGCTAGCGCGCTGCATAAACACAGCCCACG
CAAGGGTAAACCCTTTATTGCCATCAACATGGCGGCAATCCCTAAAGACTTAATTGAATCTGAGCTATTTGGCCATGAAA
AAGGTGCCTTTACTGGCGCCGGTAGCGTGCGCCAAGGCCGCTTCGAGCAAGCCAATGGCGGTACCTTATTTCTAGATGAA
ATTGGCGATATGCCTCTCGATGTTCAGACCCGATTATTGCGGGTATTGGCCGATGGCCAGTTCTATCGCGTCGGCGGCCA
TTCACCAGTGCAGGTGGATGTGCGCATCATAGCCGCAACCCACCAAAACCTTGAGCAACGGGTACATCAGGGAGGTTTTC
GTGAAGATCTGTTCCACCGTTTAAACGTCATTCGGGTACACCTGCCACCACTATCTCAACGTCGAGAGGATATCCCACAA
CTGGCACGTCACTTCCTGGTAATCGCCGCCAAAGAGATAGGCGTTGAGCCCAAGGTGCTGACCAAAGAAACTGCCAATAA
GCTGTCACAACTCCCCTGGCCCGGCAACGTGCGCCAACTTGAAAACACCTGCCGCTGGTTAACTGTCATGGCCTCGGGGC
AGGAGATCTTGCCACTGGACTTGCCACCTGAGCTACTGCAAGAACCTAAGCTGAGCCATGCTCAAAGCAGTGACTGTGAT
GACTGGCAAGGCGCATTAAAGCTGTTTATCGATCAACGCCTCAGTGACGGCGATAGCGATCTGCTTACCGAAGTGCAGCC
AGCCTTCGAGCGCATCTTACTCGAAACCGCCCTTAAACATACCAATGGCCATAAGCAAGAAGCGGCTAAACGCCTAGGCT
GGGGACGTAATACTCTAACCCGTAAATTAAAAGAGCTAGAAATGGATTAG

Upstream 100 bases:

>100_bases
CCCGCTTGCACGGTGGTCGCATCGATTGTGACTCAACAGTCGGCCACACAGAATTTACCATTACCCTACCCATCAATAAT
TAATAAGAGGAAAACACTCG

Downstream 100 bases:

>100_bases
AGGCCCTTCAATAGCGACTGAAAAAAAGGAGAGCCACAGGCTCTCCTTTTTTGCTTATACGCAAACCGCTACACGGGATC
TTGGTGAATAATCACCTCCG

Product: nitrogen metabolism transcriptional regulator NtrC

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MTEQVWILDDDSSIRWVLEKALQSAKFSSASFAAAESLWQALETAQPQVIVSDIRMPGTDGLTLLERLQNHYPHIPVIIM
TAHSDLDSAVSAYQAGAFEYLPKPFDIDEAISLVDRALTHAKEQSSTITTEEPIVATPEIIGEAPAMQEVFRAIGRLSRS
SISVLINGQSGTGKELVASALHKHSPRKGKPFIAINMAAIPKDLIESELFGHEKGAFTGAGSVRQGRFEQANGGTLFLDE
IGDMPLDVQTRLLRVLADGQFYRVGGHSPVQVDVRIIAATHQNLEQRVHQGGFREDLFHRLNVIRVHLPPLSQRREDIPQ
LARHFLVIAAKEIGVEPKVLTKETANKLSQLPWPGNVRQLENTCRWLTVMASGQEILPLDLPPELLQEPKLSHAQSSDCD
DWQGALKLFIDQRLSDGDSDLLTEVQPAFERILLETALKHTNGHKQEAAKRLGWGRNTLTRKLKELEMD

Sequences:

>Translated_469_residues
MTEQVWILDDDSSIRWVLEKALQSAKFSSASFAAAESLWQALETAQPQVIVSDIRMPGTDGLTLLERLQNHYPHIPVIIM
TAHSDLDSAVSAYQAGAFEYLPKPFDIDEAISLVDRALTHAKEQSSTITTEEPIVATPEIIGEAPAMQEVFRAIGRLSRS
SISVLINGQSGTGKELVASALHKHSPRKGKPFIAINMAAIPKDLIESELFGHEKGAFTGAGSVRQGRFEQANGGTLFLDE
IGDMPLDVQTRLLRVLADGQFYRVGGHSPVQVDVRIIAATHQNLEQRVHQGGFREDLFHRLNVIRVHLPPLSQRREDIPQ
LARHFLVIAAKEIGVEPKVLTKETANKLSQLPWPGNVRQLENTCRWLTVMASGQEILPLDLPPELLQEPKLSHAQSSDCD
DWQGALKLFIDQRLSDGDSDLLTEVQPAFERILLETALKHTNGHKQEAAKRLGWGRNTLTRKLKELEMD
>Mature_468_residues
TEQVWILDDDSSIRWVLEKALQSAKFSSASFAAAESLWQALETAQPQVIVSDIRMPGTDGLTLLERLQNHYPHIPVIIMT
AHSDLDSAVSAYQAGAFEYLPKPFDIDEAISLVDRALTHAKEQSSTITTEEPIVATPEIIGEAPAMQEVFRAIGRLSRSS
ISVLINGQSGTGKELVASALHKHSPRKGKPFIAINMAAIPKDLIESELFGHEKGAFTGAGSVRQGRFEQANGGTLFLDEI
GDMPLDVQTRLLRVLADGQFYRVGGHSPVQVDVRIIAATHQNLEQRVHQGGFREDLFHRLNVIRVHLPPLSQRREDIPQL
ARHFLVIAAKEIGVEPKVLTKETANKLSQLPWPGNVRQLENTCRWLTVMASGQEILPLDLPPELLQEPKLSHAQSSDCDD
WQGALKLFIDQRLSDGDSDLLTEVQPAFERILLETALKHTNGHKQEAAKRLGWGRNTLTRKLKELEMD

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=467, Percent_Identity=69.1648822269807, Blast_Score=662, Evalue=0.0,
Organism=Escherichia coli, GI1788550, Length=474, Percent_Identity=40.5063291139241, Blast_Score=343, Evalue=1e-95,
Organism=Escherichia coli, GI1790437, Length=466, Percent_Identity=40.7725321888412, Blast_Score=324, Evalue=8e-90,
Organism=Escherichia coli, GI1788905, Length=467, Percent_Identity=38.1156316916488, Blast_Score=291, Evalue=5e-80,
Organism=Escherichia coli, GI1789233, Length=332, Percent_Identity=43.6746987951807, Blast_Score=253, Evalue=1e-68,
Organism=Escherichia coli, GI1789087, Length=334, Percent_Identity=41.6167664670659, Blast_Score=249, Evalue=4e-67,
Organism=Escherichia coli, GI87082117, Length=335, Percent_Identity=41.7910447761194, Blast_Score=237, Evalue=9e-64,
Organism=Escherichia coli, GI1786524, Length=341, Percent_Identity=41.6422287390029, Blast_Score=234, Evalue=1e-62,
Organism=Escherichia coli, GI1787583, Length=332, Percent_Identity=39.4578313253012, Blast_Score=214, Evalue=1e-56,
Organism=Escherichia coli, GI87082152, Length=247, Percent_Identity=45.748987854251, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI87081872, Length=243, Percent_Identity=45.2674897119342, Blast_Score=200, Evalue=2e-52,
Organism=Escherichia coli, GI87081858, Length=317, Percent_Identity=30.5993690851735, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1789828, Length=230, Percent_Identity=36.9565217391304, Blast_Score=130, Evalue=3e-31,
Organism=Escherichia coli, GI1789809, Length=134, Percent_Identity=29.8507462686567, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790552, Length=106, Percent_Identity=31.1320754716981, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 52075; Mature: 51944

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEQVWILDDDSSIRWVLEKALQSAKFSSASFAAAESLWQALETAQPQVIVSDIRMPGTD
CCCEEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCC
GLTLLERLQNHYPHIPVIIMTAHSDLDSAVSAYQAGAFEYLPKPFDIDEAISLVDRALTH
CHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHHH
AKEQSSTITTEEPIVATPEIIGEAPAMQEVFRAIGRLSRSSISVLINGQSGTGKELVASA
HHHHCCCCCCCCCCEECHHHHCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHH
LHKHSPRKGKPFIAINMAAIPKDLIESELFGHEKGAFTGAGSVRQGRFEQANGGTLFLDE
HHHCCCCCCCCEEEEEHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEHH
IGDMPLDVQTRLLRVLADGQFYRVGGHSPVQVDVRIIAATHQNLEQRVHQGGFREDLFHR
HCCCCCHHHHHHHHHHHCCCEEEECCCCCEEEEEEEEEHHHHHHHHHHHCCCCHHHHHHH
LNVIRVHLPPLSQRREDIPQLARHFLVIAAKEIGVEPKVLTKETANKLSQLPWPGNVRQL
HCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCHHHH
ENTCRWLTVMASGQEILPLDLPPELLQEPKLSHAQSSDCDDWQGALKLFIDQRLSDGDSD
HHHHHEEEEEECCCEEEECCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHH
LLTEVQPAFERILLETALKHTNGHKQEAAKRLGWGRNTLTRKLKELEMD
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
TEQVWILDDDSSIRWVLEKALQSAKFSSASFAAAESLWQALETAQPQVIVSDIRMPGTD
CCEEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCC
GLTLLERLQNHYPHIPVIIMTAHSDLDSAVSAYQAGAFEYLPKPFDIDEAISLVDRALTH
CHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHHH
AKEQSSTITTEEPIVATPEIIGEAPAMQEVFRAIGRLSRSSISVLINGQSGTGKELVASA
HHHHCCCCCCCCCCEECHHHHCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHH
LHKHSPRKGKPFIAINMAAIPKDLIESELFGHEKGAFTGAGSVRQGRFEQANGGTLFLDE
HHHCCCCCCCCEEEEEHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEHH
IGDMPLDVQTRLLRVLADGQFYRVGGHSPVQVDVRIIAATHQNLEQRVHQGGFREDLFHR
HCCCCCHHHHHHHHHHHCCCEEEECCCCCEEEEEEEEEHHHHHHHHHHHCCCCHHHHHHH
LNVIRVHLPPLSQRREDIPQLARHFLVIAAKEIGVEPKVLTKETANKLSQLPWPGNVRQL
HCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCHHHH
ENTCRWLTVMASGQEILPLDLPPELLQEPKLSHAQSSDCDDWQGALKLFIDQRLSDGDSD
HHHHHEEEEEECCCEEEECCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHH
LLTEVQPAFERILLETALKHTNGHKQEAAKRLGWGRNTLTRKLKELEMD
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]