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The map label for this gene is glnL [H]

Identifier: 157963740

GI number: 157963740

Start: 4777107

End: 4778147

Strand: Direct

Name: glnL [H]

Synonym: Spea_3929

Alternate gene names: 157963740

Gene position: 4777107-4778147 (Clockwise)

Preceding gene: 157963739

Following gene: 157963741

Centisome position: 92.32

GC content: 49.28

Gene sequence:

>1041_bases
ATGGATACAAATTTATTGCTCAATCATTTAGTAACGGCGGTGCTCGTTATCAATGAAGAGCTTGAGTTACTCTATGTCAA
TACTGCTACCGAGCAATTGCTCGGTGTCGGCAGCAACCGACTCACAGAGCAACCCTTACCCGAGCACTATCAGTTACTCG
GCGTCGACCCTCAAGTCCTAAAGCTCGCCATCTGCGACGGCCAAGGCCTGAGCATCAATACCGTGCCTTTAGTGACCATA
GATGGCCAGCACCATACCGTCGATGTCACCCTGACACCGCTTGAACAAGAAAAGAATCTAGCCCTGATTGAGCTTCGCCA
AGTCGATCAACAGCGACGCATTCACCAACAGTTAACCCAAGATGCTCAGCAGCAAGCGGCGCAATACTTAGTGCGTAATC
TTGCTCATGAGATTAAGAATCCATTGGGTGGATTACGCGGCGCGGCGCAGCTATTGTCTCGCGAACTAAAAGACCCCGAG
CTCAACGAATTTACCGATCTTATTATCGAGCAGGCCGACAGACTGCGTAACTTAGTCGACCGCTTACTCGGCCCACAGAA
GCCGACCCAGCATAGCCTTTACAATATTCATGAAGTGATCCAAAAGGTGCTCAAGCTGGTAAATGTTACCCTGCCTGACA
ATATTGAATTGACCCAAGATTACGACCCATCCATCCCTGATATCGAGATGGATCCGGATCAACTGCAGCAGACCATTCTT
AATATCGTACAAAACGCGGTACAAGCCTTAGAGCCAAGCGGCGGTCATATTCGCCTAAAGACCCGTACTCAACATCAAGT
CACCATAGGTACCAAGCGCCACAAGTTGGTGCTTATGCTGTCGGTGATAGACGACGGCCCGGGCATTCAACCTGAGCTTA
TGGATACTTTGTTTTATCCTATGGTCACAGGCCGAGAACAAGGCTCTGGATTGGGCTTATCCATCGCCCATAACTTTGCC
CGCTTGCACGGTGGTCGCATCGATTGTGACTCAACAGTCGGCCACACAGAATTTACCATTACCCTACCCATCAATAATTA
A

Upstream 100 bases:

>100_bases
CGATGACCTTCTATTGCGCACTGCACCATTTCGGTGCAACATAGTGGTGTAATCGACTGAAATGCCCAAACTGGTGCATT
TGCATAATTCGGAGTAGCTG

Downstream 100 bases:

>100_bases
TAAGAGGAAAACACTCGATGACAGAACAAGTTTGGATCCTCGACGACGATAGTTCGATACGTTGGGTACTAGAAAAAGCA
CTACAGAGCGCCAAATTTAG

Product: signal transduction histidine kinase, nitrogen specific, NtrB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MDTNLLLNHLVTAVLVINEELELLYVNTATEQLLGVGSNRLTEQPLPEHYQLLGVDPQVLKLAICDGQGLSINTVPLVTI
DGQHHTVDVTLTPLEQEKNLALIELRQVDQQRRIHQQLTQDAQQQAAQYLVRNLAHEIKNPLGGLRGAAQLLSRELKDPE
LNEFTDLIIEQADRLRNLVDRLLGPQKPTQHSLYNIHEVIQKVLKLVNVTLPDNIELTQDYDPSIPDIEMDPDQLQQTIL
NIVQNAVQALEPSGGHIRLKTRTQHQVTIGTKRHKLVLMLSVIDDGPGIQPELMDTLFYPMVTGREQGSGLGLSIAHNFA
RLHGGRIDCDSTVGHTEFTITLPINN

Sequences:

>Translated_346_residues
MDTNLLLNHLVTAVLVINEELELLYVNTATEQLLGVGSNRLTEQPLPEHYQLLGVDPQVLKLAICDGQGLSINTVPLVTI
DGQHHTVDVTLTPLEQEKNLALIELRQVDQQRRIHQQLTQDAQQQAAQYLVRNLAHEIKNPLGGLRGAAQLLSRELKDPE
LNEFTDLIIEQADRLRNLVDRLLGPQKPTQHSLYNIHEVIQKVLKLVNVTLPDNIELTQDYDPSIPDIEMDPDQLQQTIL
NIVQNAVQALEPSGGHIRLKTRTQHQVTIGTKRHKLVLMLSVIDDGPGIQPELMDTLFYPMVTGREQGSGLGLSIAHNFA
RLHGGRIDCDSTVGHTEFTITLPINN
>Mature_346_residues
MDTNLLLNHLVTAVLVINEELELLYVNTATEQLLGVGSNRLTEQPLPEHYQLLGVDPQVLKLAICDGQGLSINTVPLVTI
DGQHHTVDVTLTPLEQEKNLALIELRQVDQQRRIHQQLTQDAQQQAAQYLVRNLAHEIKNPLGGLRGAAQLLSRELKDPE
LNEFTDLIIEQADRLRNLVDRLLGPQKPTQHSLYNIHEVIQKVLKLVNVTLPDNIELTQDYDPSIPDIEMDPDQLQQTIL
NIVQNAVQALEPSGGHIRLKTRTQHQVTIGTKRHKLVLMLSVIDDGPGIQPELMDTLFYPMVTGREQGSGLGLSIAHNFA
RLHGGRIDCDSTVGHTEFTITLPINN

Specific function: NtrB acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of ntrC. In nitrogen limitation ntrB activates ntrC by phosphorylating it, while in nitrogen excess ntrC is dephosphorylated and consequently inactiva

COG id: COG3852

COG function: function code T; Signal transduction histidine kinase, nitrogen specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790300, Length=345, Percent_Identity=51.304347826087, Blast_Score=340, Evalue=1e-94,
Organism=Escherichia coli, GI1790436, Length=252, Percent_Identity=31.3492063492063, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI1788549, Length=353, Percent_Identity=25.2124645892351, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI87081816, Length=249, Percent_Identity=27.3092369477912, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1786600, Length=354, Percent_Identity=27.4011299435028, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1790861, Length=241, Percent_Identity=30.2904564315353, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 38648; Mature: 38648

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTNLLLNHLVTAVLVINEELELLYVNTATEQLLGVGSNRLTEQPLPEHYQLLGVDPQVL
CCHHHHHHHHHHHHHHCCCCEEEEEEECHHHHHHCCCCCCCCCCCCCCHHEEECCCHHHE
KLAICDGQGLSINTVPLVTIDGQHHTVDVTLTPLEQEKNLALIELRQVDQQRRIHQQLTQ
EEEEECCCCCEEEEEEEEEECCCCEEEEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHH
DAQQQAAQYLVRNLAHEIKNPLGGLRGAAQLLSRELKDPELNEFTDLIIEQADRLRNLVD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
RLLGPQKPTQHSLYNIHEVIQKVLKLVNVTLPDNIELTQDYDPSIPDIEMDPDQLQQTIL
HHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCCCCHHHHHHHHH
NIVQNAVQALEPSGGHIRLKTRTQHQVTIGTKRHKLVLMLSVIDDGPGIQPELMDTLFYP
HHHHHHHHHHCCCCCEEEEEECCCEEEEECCCCCEEEEEEEEECCCCCCCHHHHHHHHHH
MVTGREQGSGLGLSIAHNFARLHGGRIDCDSTVGHTEFTITLPINN
HHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCC
>Mature Secondary Structure
MDTNLLLNHLVTAVLVINEELELLYVNTATEQLLGVGSNRLTEQPLPEHYQLLGVDPQVL
CCHHHHHHHHHHHHHHCCCCEEEEEEECHHHHHHCCCCCCCCCCCCCCHHEEECCCHHHE
KLAICDGQGLSINTVPLVTIDGQHHTVDVTLTPLEQEKNLALIELRQVDQQRRIHQQLTQ
EEEEECCCCCEEEEEEEEEECCCCEEEEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHH
DAQQQAAQYLVRNLAHEIKNPLGGLRGAAQLLSRELKDPELNEFTDLIIEQADRLRNLVD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
RLLGPQKPTQHSLYNIHEVIQKVLKLVNVTLPDNIELTQDYDPSIPDIEMDPDQLQQTIL
HHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCCCCHHHHHHHHH
NIVQNAVQALEPSGGHIRLKTRTQHQVTIGTKRHKLVLMLSVIDDGPGIQPELMDTLFYP
HHHHHHHHHHCCCCCEEEEEECCCEEEEECCCCCEEEEEEEEECCCCCCCHHHHHHHHHH
MVTGREQGSGLGLSIAHNFARLHGGRIDCDSTVGHTEFTITLPINN
HHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]