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The map label for this gene is hemB [H]

Identifier: 157963613

GI number: 157963613

Start: 4615072

End: 4616082

Strand: Direct

Name: hemB [H]

Synonym: Spea_3801

Alternate gene names: 157963613

Gene position: 4615072-4616082 (Clockwise)

Preceding gene: 157963612

Following gene: 157963616

Centisome position: 89.19

GC content: 45.8

Gene sequence:

>1011_bases
GTGAATATTATTACTAGTGCCTACCCACAACGTAGAATGCGCCGTATGCGTAAACATGAGTTTAGTCGCCGTCTAATGGC
TGAAAATCAGCTATCAGTAAATGACTTAATCTACCCTATGTTTGTCTTAGAAGGTAACAACCGCACAGAGCAAGTTGCTT
CAATGCCTGGTGTAGAGCGCCTTTCAATTGACCTATTACTTAAAGAAGCTGAAGAGCTTGTTGAGCTAGGTATTCCTCTA
ATTGCATTATTCCCAGTCACTCCAGCTGAGAAGAAAACTTTAATGGCTGAAGAAGCCTATAATGCCGATGCGCTAGCCCA
ACGTGCAGTTCGTGCACTGAAAGAAGCATTCCCTCAGCTTGGCGTCATGACAGACGTTGCACTCGACCCATTCACGACAC
ATGGTCAAGATGGGATTATCGATGATACTGGCTATATCATGAATGATATCACCACAGAGATCTTGGTTAAACAGGCCCTG
TCTCATGCTGAAGCTGGAGCTGATATCGTCGCCCCCTCAGACATGATGGATGGCCGTATTGGTGCTATTCGTAAAGCACT
TGAAGAAGCTGGCCATGTGAATACACAGATCATGGCGTACTCAGCAAAGTACTCTTCTAATTACTACGGCCCTTTCCGTG
ATGCAGTTGGCTCTGCAGGCAACCTAAAAGGTGGCAATAAGCATAGCTACCAGATGGATCCAGCAAATAGCGATGAAGCA
CTACATGAAGTTGCTTTAGACATCCAAGAAGGCGCTGATATGGTCATGGTTAAGCCTGGTATGCCATACCTAGACATAGT
GCATCGCGTAAAGACCGAACTAGCAGTACCTACGTTTGCTTACCAGGTAAGTGGCGAATACGCGATGCATATGGCAGCGA
TTCAAAACGGTTGGTTAGCTGAGAAGGCGATCGTTATGGAATCGCTACTTTGCTTTAAGCGTGCAGGCGCCGATGGCATC
CTAACCTATTTCGCTAAACGTGCCGCTCAATGGTTAAAAGAAGACAAATAA

Upstream 100 bases:

>100_bases
AGCAGATAAAGCACTTTATCAAGCAAAGAATGACGGGCGTAACCGAGTAAGACTCTACAGCCCCGATCAAGATTAATATC
ACCATCGTCTGGAGCCAAAT

Downstream 100 bases:

>100_bases
TCCTGTCTGAATTTAAACCTGTCTAGTTCTAAAATAGGTTGACGGTTTTTATTAAGCCAGTTGGTATTCCCAACTGGCTT
TTTTCATGCCCTACTCCACA

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MNIITSAYPQRRMRRMRKHEFSRRLMAENQLSVNDLIYPMFVLEGNNRTEQVASMPGVERLSIDLLLKEAEELVELGIPL
IALFPVTPAEKKTLMAEEAYNADALAQRAVRALKEAFPQLGVMTDVALDPFTTHGQDGIIDDTGYIMNDITTEILVKQAL
SHAEAGADIVAPSDMMDGRIGAIRKALEEAGHVNTQIMAYSAKYSSNYYGPFRDAVGSAGNLKGGNKHSYQMDPANSDEA
LHEVALDIQEGADMVMVKPGMPYLDIVHRVKTELAVPTFAYQVSGEYAMHMAAIQNGWLAEKAIVMESLLCFKRAGADGI
LTYFAKRAAQWLKEDK

Sequences:

>Translated_336_residues
MNIITSAYPQRRMRRMRKHEFSRRLMAENQLSVNDLIYPMFVLEGNNRTEQVASMPGVERLSIDLLLKEAEELVELGIPL
IALFPVTPAEKKTLMAEEAYNADALAQRAVRALKEAFPQLGVMTDVALDPFTTHGQDGIIDDTGYIMNDITTEILVKQAL
SHAEAGADIVAPSDMMDGRIGAIRKALEEAGHVNTQIMAYSAKYSSNYYGPFRDAVGSAGNLKGGNKHSYQMDPANSDEA
LHEVALDIQEGADMVMVKPGMPYLDIVHRVKTELAVPTFAYQVSGEYAMHMAAIQNGWLAEKAIVMESLLCFKRAGADGI
LTYFAKRAAQWLKEDK
>Mature_336_residues
MNIITSAYPQRRMRRMRKHEFSRRLMAENQLSVNDLIYPMFVLEGNNRTEQVASMPGVERLSIDLLLKEAEELVELGIPL
IALFPVTPAEKKTLMAEEAYNADALAQRAVRALKEAFPQLGVMTDVALDPFTTHGQDGIIDDTGYIMNDITTEILVKQAL
SHAEAGADIVAPSDMMDGRIGAIRKALEEAGHVNTQIMAYSAKYSSNYYGPFRDAVGSAGNLKGGNKHSYQMDPANSDEA
LHEVALDIQEGADMVMVKPGMPYLDIVHRVKTELAVPTFAYQVSGEYAMHMAAIQNGWLAEKAIVMESLLCFKRAGADGI
LTYFAKRAAQWLKEDK

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=311, Percent_Identity=41.4790996784566, Blast_Score=236, Evalue=3e-62,
Organism=Escherichia coli, GI87081728, Length=315, Percent_Identity=44.4444444444444, Blast_Score=238, Evalue=5e-64,
Organism=Saccharomyces cerevisiae, GI6321398, Length=324, Percent_Identity=37.3456790123457, Blast_Score=202, Evalue=7e-53,
Organism=Drosophila melanogaster, GI21358291, Length=320, Percent_Identity=39.0625, Blast_Score=224, Evalue=7e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 37175; Mature: 37175

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
5.7 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
5.7 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIITSAYPQRRMRRMRKHEFSRRLMAENQLSVNDLIYPMFVLEGNNRTEQVASMPGVER
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHEEEEEEEECCCHHHHHHHCCCCHH
LSIDLLLKEAEELVELGIPLIALFPVTPAEKKTLMAEEAYNADALAQRAVRALKEAFPQL
HHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHC
GVMTDVALDPFTTHGQDGIIDDTGYIMNDITTEILVKQALSHAEAGADIVAPSDMMDGRI
CCHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCHH
GAIRKALEEAGHVNTQIMAYSAKYSSNYYGPFRDAVGSAGNLKGGNKHSYQMDPANSDEA
HHHHHHHHHHCCCCEEEEEEEECCCCCCCCCHHHHCCCCCCCCCCCCCCEECCCCCCHHH
LHEVALDIQEGADMVMVKPGMPYLDIVHRVKTELAVPTFAYQVSGEYAMHMAAIQNGWLA
HHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCHHEEEECCHHHHHHHHHHCCCHH
EKAIVMESLLCFKRAGADGILTYFAKRAAQWLKEDK
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNIITSAYPQRRMRRMRKHEFSRRLMAENQLSVNDLIYPMFVLEGNNRTEQVASMPGVER
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHEEEEEEEECCCHHHHHHHCCCCHH
LSIDLLLKEAEELVELGIPLIALFPVTPAEKKTLMAEEAYNADALAQRAVRALKEAFPQL
HHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHC
GVMTDVALDPFTTHGQDGIIDDTGYIMNDITTEILVKQALSHAEAGADIVAPSDMMDGRI
CCHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCHH
GAIRKALEEAGHVNTQIMAYSAKYSSNYYGPFRDAVGSAGNLKGGNKHSYQMDPANSDEA
HHHHHHHHHHCCCCEEEEEEEECCCCCCCCCHHHHCCCCCCCCCCCCCCEECCCCCCHHH
LHEVALDIQEGADMVMVKPGMPYLDIVHRVKTELAVPTFAYQVSGEYAMHMAAIQNGWLA
HHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCHHEEEECCHHHHHHHHHHCCCHH
EKAIVMESLLCFKRAGADGILTYFAKRAAQWLKEDK
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9529530; 10984043; 10356331; 12079382 [H]