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The map label for this gene is maeB [H]

Identifier: 157963586

GI number: 157963586

Start: 4588590

End: 4589840

Strand: Reverse

Name: maeB [H]

Synonym: Spea_3774

Alternate gene names: 157963586

Gene position: 4589840-4588590 (Counterclockwise)

Preceding gene: 157963587

Following gene: 157963585

Centisome position: 88.7

GC content: 45.96

Gene sequence:

>1251_bases
ATGGCAGATCTTCGTCAACAAGCCCTCGATTATCATGAATTCCCAGTTCCAGGCAAAACAGCAGTTTGCCTAACCAAGCC
CGCAGAAACCAGCATGGACCTAGCGCTAGCATATAGCCCTGGCGTAGCTGAACCTGTCCGTGAAATTGCGGCTAATCCAG
ACAATGCGTACCGCTATACAGGTAAAGGCAATACTGTTGCTGTTATCTCAAATGGTACGGCAATCTTAGGTTTAGGGAAT
TTAGGCCCGTTGGCCTCTAAGCCTGTTATGGAAGGTAAAGCGCTACTTTTCAAACACTTTGCGAATATCGATGCTACAGA
CATTGAAGTTAAACACAGAACGTCTGAAGAGTTCATCAACGTAGTTGAGGCGATTGCCGACACGTTTGGTGGTATTAACC
TTGAAGATATCAAGGCACCAGAGTGTTTTGAGATTGAAAAAGCACTGATTGAGCGTTGTAACGTACCAGTATTCCACGAT
GATCAGCATGGTACTGCGATTGTGACTGCGGCCGGTATGATTAACGCACTGGAAATTCAAGGCAAGTCTATCGAAGAGGC
TGTGTTTGTATGTATGGGCGCCGGCGCTGCAGCTATTGCCTGTATGACTATGATTGTGAACTGTGGCGCTCAGCGCGAAA
ACGTTTACATGCTAGATAGAAAAGGCGTTATTCATACTCGTCGTGAAGACATCAACGAGTACAAGGCGCTATTTGCCAAC
AATACAGATAAGCGCACCCTGCAGGATGTGATTAAAGGTGCTGACGTATTCTTAGGTCTTTCTGGCCCAGATGTATTAGG
CGCTGAAGATGTGGCATTGATGGCCGATAAGCCAGTTATTTTTGCTTGTTCTAACCCAGATCCTGAGATCAAGCCTGAAC
TGGCACACGACGTGCGTAAAGATTTGATCATGGGGACGGGTCGTAGTGATTATCCTAACCAAGTTAACAATGTACTTTGC
TTCCCATTCATTTTCCGTGGTGCGCTAGATGTTCGTGCTTCAAGTATTAATGATGAGATGAAGATTGCAGCGGTACATGC
TATTGCAGCATTAGCTAAAGAGCCTGTTCCTGCGTCTGTGCTTGCAGCTTACCCAAATGTTGACTCGCTAGAGTTTGGTC
CTGAGTACGTGATCCCTAAACCAATCGATGCACGTCTTCTACCAAACGTTGCTAAAGCCGTTGCTATTGCAGCAATTGAA
TCGGGTGTAGCGACAATTAAAGAGCTACCAGAAGGCTACATGGAGTCTTAA

Upstream 100 bases:

>100_bases
AAAGAACCTTTTAAATGTATCTTTATTGAGCAGCATGATTAACCTTTTAGTTGTCGACCTTTCGCTCAGGATATTATTAG
TCGCACTCAGGATTAGAAAA

Downstream 100 bases:

>100_bases
TCACTTGGTGCCATACTCAAGCGACTGAATTCTGTATCTTCAGGTGGCTTGGGCATACATCATAAAATAAGGGCCTAGTG
GCCCTTTTTTGTTGCCGAGC

Product: malate dehydrogenase

Products: NA

Alternate protein names: NADP-ME [H]

Number of amino acids: Translated: 416; Mature: 415

Protein sequence:

>416_residues
MADLRQQALDYHEFPVPGKTAVCLTKPAETSMDLALAYSPGVAEPVREIAANPDNAYRYTGKGNTVAVISNGTAILGLGN
LGPLASKPVMEGKALLFKHFANIDATDIEVKHRTSEEFINVVEAIADTFGGINLEDIKAPECFEIEKALIERCNVPVFHD
DQHGTAIVTAAGMINALEIQGKSIEEAVFVCMGAGAAAIACMTMIVNCGAQRENVYMLDRKGVIHTRREDINEYKALFAN
NTDKRTLQDVIKGADVFLGLSGPDVLGAEDVALMADKPVIFACSNPDPEIKPELAHDVRKDLIMGTGRSDYPNQVNNVLC
FPFIFRGALDVRASSINDEMKIAAVHAIAALAKEPVPASVLAAYPNVDSLEFGPEYVIPKPIDARLLPNVAKAVAIAAIE
SGVATIKELPEGYMES

Sequences:

>Translated_416_residues
MADLRQQALDYHEFPVPGKTAVCLTKPAETSMDLALAYSPGVAEPVREIAANPDNAYRYTGKGNTVAVISNGTAILGLGN
LGPLASKPVMEGKALLFKHFANIDATDIEVKHRTSEEFINVVEAIADTFGGINLEDIKAPECFEIEKALIERCNVPVFHD
DQHGTAIVTAAGMINALEIQGKSIEEAVFVCMGAGAAAIACMTMIVNCGAQRENVYMLDRKGVIHTRREDINEYKALFAN
NTDKRTLQDVIKGADVFLGLSGPDVLGAEDVALMADKPVIFACSNPDPEIKPELAHDVRKDLIMGTGRSDYPNQVNNVLC
FPFIFRGALDVRASSINDEMKIAAVHAIAALAKEPVPASVLAAYPNVDSLEFGPEYVIPKPIDARLLPNVAKAVAIAAIE
SGVATIKELPEGYMES
>Mature_415_residues
ADLRQQALDYHEFPVPGKTAVCLTKPAETSMDLALAYSPGVAEPVREIAANPDNAYRYTGKGNTVAVISNGTAILGLGNL
GPLASKPVMEGKALLFKHFANIDATDIEVKHRTSEEFINVVEAIADTFGGINLEDIKAPECFEIEKALIERCNVPVFHDD
QHGTAIVTAAGMINALEIQGKSIEEAVFVCMGAGAAAIACMTMIVNCGAQRENVYMLDRKGVIHTRREDINEYKALFANN
TDKRTLQDVIKGADVFLGLSGPDVLGAEDVALMADKPVIFACSNPDPEIKPELAHDVRKDLIMGTGRSDYPNQVNNVLCF
PFIFRGALDVRASSINDEMKIAAVHAIAALAKEPVPASVLAAYPNVDSLEFGPEYVIPKPIDARLLPNVAKAVAIAAIES
GVATIKELPEGYMES

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the phosphate acetyltransferase and butyryltransferase family [H]

Homologues:

Organism=Homo sapiens, GI62420882, Length=350, Percent_Identity=26.2857142857143, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI62420880, Length=350, Percent_Identity=26.2857142857143, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI239049447, Length=350, Percent_Identity=26.2857142857143, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI4505143, Length=343, Percent_Identity=24.7813411078717, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI4505145, Length=371, Percent_Identity=25.6064690026954, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1788806, Length=404, Percent_Identity=62.1287128712871, Blast_Score=479, Evalue=1e-136,
Organism=Escherichia coli, GI87081919, Length=242, Percent_Identity=26.0330578512397, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17537199, Length=367, Percent_Identity=25.8855585831063, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6322823, Length=328, Percent_Identity=25.9146341463415, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI78707242, Length=401, Percent_Identity=25.9351620947631, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI78707238, Length=401, Percent_Identity=25.9351620947631, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI78707240, Length=339, Percent_Identity=26.8436578171091, Blast_Score=86, Evalue=7e-17,
Organism=Drosophila melanogaster, GI281362674, Length=409, Percent_Identity=26.6503667481663, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI281362672, Length=409, Percent_Identity=26.6503667481663, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21356279, Length=408, Percent_Identity=26.2254901960784, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI281363503, Length=357, Percent_Identity=22.96918767507, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI281363505, Length=357, Percent_Identity=22.96918767507, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI78707236, Length=357, Percent_Identity=22.96918767507, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI78707232, Length=357, Percent_Identity=22.96918767507, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24646388, Length=365, Percent_Identity=27.3972602739726, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24646386, Length=365, Percent_Identity=27.3972602739726, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR012188
- InterPro:   IPR016040
- InterPro:   IPR002505 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M; PF01515 PTA_PTB [H]

EC number: =1.1.1.40 [H]

Molecular weight: Translated: 44620; Mature: 44488

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS00331 MALIC_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADLRQQALDYHEFPVPGKTAVCLTKPAETSMDLALAYSPGVAEPVREIAANPDNAYRYT
CCCHHHHHHCCCCCCCCCCEEEEEECCCCCCCEEEEEECCCHHHHHHHHHCCCCCCEEEE
GKGNTVAVISNGTAILGLGNLGPLASKPVMEGKALLFKHFANIDATDIEVKHRTSEEFIN
CCCCEEEEEECCEEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCHHHHHH
VVEAIADTFGGINLEDIKAPECFEIEKALIERCNVPVFHDDQHGTAIVTAAGMINALEIQ
HHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHCCCCEEECCCCCCEEEEECCCEEEEEEC
GKSIEEAVFVCMGAGAAAIACMTMIVNCGAQRENVYMLDRKGVIHTRREDINEYKALFAN
CCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCC
NTDKRTLQDVIKGADVFLGLSGPDVLGAEDVALMADKPVIFACSNPDPEIKPELAHDVRK
CCCHHHHHHHHCCCCEEEECCCCCCCCCCCEEEEECCCEEEEECCCCCCCCCHHHHHHHH
DLIMGTGRSDYPNQVNNVLCFPFIFRGALDVRASSINDEMKIAAVHAIAALAKEPVPASV
HHEECCCCCCCCHHHHCEEEEHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHH
LAAYPNVDSLEFGPEYVIPKPIDARLLPNVAKAVAIAAIESGVATIKELPEGYMES
HEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCC
>Mature Secondary Structure 
ADLRQQALDYHEFPVPGKTAVCLTKPAETSMDLALAYSPGVAEPVREIAANPDNAYRYT
CCHHHHHHCCCCCCCCCCEEEEEECCCCCCCEEEEEECCCHHHHHHHHHCCCCCCEEEE
GKGNTVAVISNGTAILGLGNLGPLASKPVMEGKALLFKHFANIDATDIEVKHRTSEEFIN
CCCCEEEEEECCEEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCHHHHHH
VVEAIADTFGGINLEDIKAPECFEIEKALIERCNVPVFHDDQHGTAIVTAAGMINALEIQ
HHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHCCCCEEECCCCCCEEEEECCCEEEEEEC
GKSIEEAVFVCMGAGAAAIACMTMIVNCGAQRENVYMLDRKGVIHTRREDINEYKALFAN
CCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCC
NTDKRTLQDVIKGADVFLGLSGPDVLGAEDVALMADKPVIFACSNPDPEIKPELAHDVRK
CCCHHHHHHHHCCCCEEEECCCCCCCCCCCEEEEECCCEEEEECCCCCCCCCHHHHHHHH
DLIMGTGRSDYPNQVNNVLCFPFIFRGALDVRASSINDEMKIAAVHAIAALAKEPVPASV
HHEECCCCCCCCHHHHCEEEEHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHH
LAAYPNVDSLEFGPEYVIPKPIDARLLPNVAKAVAIAAIESGVATIKELPEGYMES
HEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 36376 [H]