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The map label for this gene is yafV [C]

Identifier: 157963560

GI number: 157963560

Start: 4557422

End: 4558261

Strand: Reverse

Name: yafV [C]

Synonym: Spea_3748

Alternate gene names: 157963560

Gene position: 4558261-4557422 (Counterclockwise)

Preceding gene: 157963561

Following gene: 157963559

Centisome position: 88.09

GC content: 52.02

Gene sequence:

>840_bases
ATGCAAGTCAGCCTACTGCAATGTCAAAGCAGCCGCAACGTAGCGGACAATCTAGCGTTTATCGAGTCGCAGCTTAATCA
ATTACCGCGCCATAGCGATGAGCCGCAACTGGTGGTTTTGCCAGAATGTTGCCTACTGTTCGGAGGTTATGAAAGCCAGC
AACTAGAATATGCGACCGACGCAGAGCAAAGCCCGCTTAGACAGGCACTGTCTGAGTTAGCGAAACGCTATCGGGTAGTG
ATGGTGGCGGGGAGTATTCCGATTTTGGCCGAAGATGGTCGGGTCTACAATCGCTGCTATGTATTTGATACAGACGGTAG
CATATTGGGCCAGTACGATAAGCTGCACCTGTTCGATGTCGAGGTGACTGATGGCACTAAGCAGTATCGCGAGAGCGATG
CATTCTGCCCGGGTGAGCAGATAGTTGTGGTCGATACGCCAATGGGTAAAGTGGGGCTGGCAATCTGTTACGACATACGT
TTCCCAGATCTTTTTAGGGCTCTGCGAGAAGCTGGAGCCGAGCTTATTGCCGTTCCTGCCGCATTCACTAAGGTGACGGG
GCAAGCTCATTGGCAGATACTACTACAGGCCCGCGCTATCGAAACCCAGTGCTTTATTTTAGGTGCAGCCCAGTGGGGGC
AACATAATCAGGGTAGCCGAGAGACCTGGGGGCAAAGTATGATAGTCAGCCCGTGGGGAGAGATTCTAGTGCAAAAGCTG
CAGGGAACCGGTTGGCTACAAGCGCAAGTGGAGCTGCAAAGCTTGCAAGATATCCGCGCTAAGATGCCTGTGATGACGCA
TAATCGATTTGCCAAGCCAGTACTTAAGTCGCACAGCTAA

Upstream 100 bases:

>100_bases
CAAGCCGAGTCATCATTGAGCGAGTCATCACTGGCTGAGTCGCCATTGAACGAATCGTTATTGAACCAGACATCGGACGG
GAAACTAGAGGGAGATGTAG

Downstream 100 bases:

>100_bases
GACCAAATACTAAGAGCAAGTCGAACCATAAATTTAAGGTAAATTGAGGCTCACGCGTGAGACTCTGTTTACCGAAATGT
TTTATCGAATCAATATGAAG

Product: Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MQVSLLQCQSSRNVADNLAFIESQLNQLPRHSDEPQLVVLPECCLLFGGYESQQLEYATDAEQSPLRQALSELAKRYRVV
MVAGSIPILAEDGRVYNRCYVFDTDGSILGQYDKLHLFDVEVTDGTKQYRESDAFCPGEQIVVVDTPMGKVGLAICYDIR
FPDLFRALREAGAELIAVPAAFTKVTGQAHWQILLQARAIETQCFILGAAQWGQHNQGSRETWGQSMIVSPWGEILVQKL
QGTGWLQAQVELQSLQDIRAKMPVMTHNRFAKPVLKSHS

Sequences:

>Translated_279_residues
MQVSLLQCQSSRNVADNLAFIESQLNQLPRHSDEPQLVVLPECCLLFGGYESQQLEYATDAEQSPLRQALSELAKRYRVV
MVAGSIPILAEDGRVYNRCYVFDTDGSILGQYDKLHLFDVEVTDGTKQYRESDAFCPGEQIVVVDTPMGKVGLAICYDIR
FPDLFRALREAGAELIAVPAAFTKVTGQAHWQILLQARAIETQCFILGAAQWGQHNQGSRETWGQSMIVSPWGEILVQKL
QGTGWLQAQVELQSLQDIRAKMPVMTHNRFAKPVLKSHS
>Mature_279_residues
MQVSLLQCQSSRNVADNLAFIESQLNQLPRHSDEPQLVVLPECCLLFGGYESQQLEYATDAEQSPLRQALSELAKRYRVV
MVAGSIPILAEDGRVYNRCYVFDTDGSILGQYDKLHLFDVEVTDGTKQYRESDAFCPGEQIVVVDTPMGKVGLAICYDIR
FPDLFRALREAGAELIAVPAAFTKVTGQAHWQILLQARAIETQCFILGAAQWGQHNQGSRETWGQSMIVSPWGEILVQKL
QGTGWLQAQVELQSLQDIRAKMPVMTHNRFAKPVLKSHS

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=277, Percent_Identity=35.3790613718412, Blast_Score=168, Evalue=4e-42,
Organism=Homo sapiens, GI5031947, Length=177, Percent_Identity=43.5028248587571, Blast_Score=167, Evalue=8e-42,
Organism=Homo sapiens, GI297632348, Length=177, Percent_Identity=43.5028248587571, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI297632350, Length=177, Percent_Identity=43.5028248587571, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI297632346, Length=100, Percent_Identity=44, Blast_Score=96, Evalue=4e-20,
Organism=Escherichia coli, GI1786412, Length=185, Percent_Identity=30.8108108108108, Blast_Score=73, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17556280, Length=273, Percent_Identity=35.5311355311355, Blast_Score=171, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6323383, Length=280, Percent_Identity=31.7857142857143, Blast_Score=166, Evalue=4e-42,
Organism=Saccharomyces cerevisiae, GI6322335, Length=304, Percent_Identity=34.2105263157895, Blast_Score=161, Evalue=1e-40,
Organism=Drosophila melanogaster, GI17933642, Length=265, Percent_Identity=40, Blast_Score=174, Evalue=6e-44,
Organism=Drosophila melanogaster, GI21355835, Length=275, Percent_Identity=30.9090909090909, Blast_Score=166, Evalue=2e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010
- InterPro:   IPR001110 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: 3.5.-.- [C]

Molecular weight: Translated: 31213; Mature: 31213

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS50263 CN_HYDROLASE ; PS01227 UPF0012

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVSLLQCQSSRNVADNLAFIESQLNQLPRHSDEPQLVVLPECCLLFGGYESQQLEYATD
CCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEECCHHHHHHCCCCCCCEEECCC
AEQSPLRQALSELAKRYRVVMVAGSIPILAEDGRVYNRCYVFDTDGSILGQYDKLHLFDV
CCHHHHHHHHHHHHHHEEEEEEECCCCEEECCCCEEEEEEEEECCCCEECCCCEEEEEEE
EVTDGTKQYRESDAFCPGEQIVVVDTPMGKVGLAICYDIRFPDLFRALREAGAELIAVPA
EECCCHHHHHCCCCCCCCCEEEEEECCCCCEEEEEEEECCCHHHHHHHHHCCCCEEEECH
AFTKVTGQAHWQILLQARAIETQCFILGAAQWGQHNQGSRETWGQSMIVSPWGEILVQKL
HHHHHCCCCEEEEEEEHHHCCEEEEEEECHHCCCCCCCCHHHCCCEEEECCHHHHHHHHH
QGTGWLQAQVELQSLQDIRAKMPVMTHNRFAKPVLKSHS
CCCCEEEEEEHHHHHHHHHHHCCCHHCCHHHHHHHHCCC
>Mature Secondary Structure
MQVSLLQCQSSRNVADNLAFIESQLNQLPRHSDEPQLVVLPECCLLFGGYESQQLEYATD
CCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEECCHHHHHHCCCCCCCEEECCC
AEQSPLRQALSELAKRYRVVMVAGSIPILAEDGRVYNRCYVFDTDGSILGQYDKLHLFDV
CCHHHHHHHHHHHHHHEEEEEEECCCCEEECCCCEEEEEEEEECCCCEECCCCEEEEEEE
EVTDGTKQYRESDAFCPGEQIVVVDTPMGKVGLAICYDIRFPDLFRALREAGAELIAVPA
EECCCHHHHHCCCCCCCCCEEEEEECCCCCEEEEEEEECCCHHHHHHHHHCCCCEEEECH
AFTKVTGQAHWQILLQARAIETQCFILGAAQWGQHNQGSRETWGQSMIVSPWGEILVQKL
HHHHHCCCCEEEEEEEHHHCCEEEEEEECHHCCCCCCCCHHHCCCEEEECCHHHHHHHHH
QGTGWLQAQVELQSLQDIRAKMPVMTHNRFAKPVLKSHS
CCCCEEEEEEHHHHHHHHHHHCCCHHCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]