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The map label for this gene is yaaJ [C]

Identifier: 157963546

GI number: 157963546

Start: 4537898

End: 4539277

Strand: Reverse

Name: yaaJ [C]

Synonym: Spea_3734

Alternate gene names: 157963546

Gene position: 4539277-4537898 (Counterclockwise)

Preceding gene: 157963547

Following gene: 157963540

Centisome position: 87.72

GC content: 47.9

Gene sequence:

>1380_bases
ATGGAAGCTATTACGAATTTTGTAAGCATGATCAATGGCGTGGTCTGGGGGACTCCGATGTTGATTATGATTCTGGGGGT
CGGGTTACTCTTGACCATTGGGTTGCGGTTTATGCCGATTTTAAAGTTAGGCACGGGTTTTAAGCTGCTATGGTCTGGGC
GTATTCCGGATAAAGATAAGAACATGAAAGGGGAGATTAGCCCTTTTAATGCTTTGATGACGTCACTGTCGGCGACTATA
GGTACAGGTAATATTGCCGGTGTGGCTACCGCGATTTTTATTGGTGGTCCAGGTGCTTTGTTTTGGATGTGGTGTACGGC
GTTGGTAGGAATGGCGACTAAGTTTGCCGAAGCCGTGCTTGCGGTTAAGTATCGTGAAGTGGATGACAATGGTAACCACG
TTGGTGGTCCAATGTACTACATCAAAAATGGTTTAGGGGCTAAATGGGCTTGGCTAGGTACCGCCTTTGCACTATTTGGT
TCTCTTGCCGGTTTTGGTATCGGTAATACGGTTCAGGCTAACTCGGTTGCCGATGCACTCAGTAGTAATTTTGGCGTGCC
AAATTGGATTACGGGTGTGGTACTTATGGTGCTGGTTGGCGCTGTACTTATGGGCGGAATTAAGCGTATTGCCGATGTTG
CCGGCAAGCTCGTGCCAATGATGACGATTTTCTATCTCGCCGCAGGTGTTGCGGTTTTGGTGGTTTACGCTTCTGAGATC
CCTGCGGCAATTGAGCTGATTTTGCATAGCGCATTTAACCCTGTTGCTGCGCAAGGTGGCTTTGCTGGAGCGGCGGTTTG
GGCTGCGATTCGTTTTGGTGTGGCACGCGGTGTGTTCTCTAATGAAGCTGGCCTAGGTAGTGCGCCGATTGCCCATGCTG
CGGCGCAGACTAACAATCCAGTGGCACAAGGTTTAGTGGCCATGCTAGGCACATTTATCGATACCTTGATTGTTTGTAGT
ATCACAGGTTTGGCTATTATCGTTTCAGGTGCTTGGACTTCGGGTGAGAACGGCGCAGCGTTAACCTCTTTTGCTTTCTC
TCATGCTTTCCCTATGGGTAACTATGTAGTGGCAATTGCTCTGAGTATTTTCGCCTTTACTACGATTTTGGGCTGGAGCT
TTTACTGTGAGAAGTGCGTGCAGTTCCTGTTTGGCATTAAAGCCATCAAGCCATTTAGAATTCTTTGGACCATAGTGGTG
CCGTTAGGAGCAGTGAGTTCGCTGGAGTTTATTTGGTTATTGGCCGATACGCTCAATGCCATGATGGCAATTCCCAACCT
GATCGCCTTGGCACTATTGAGTCCTGTGGTATTTGCACTTACCAGAGAGTACTTCTTGAAGAAGCGTCAGACTGATTTAG
AATCGCAAAAAGCCAAATAG

Upstream 100 bases:

>100_bases
AATTCAAGATCAAAAATGCAAGATCAAAAAGCCCTAGAGACAAAAACACAATTATAAATATAAATAATAACGATAAATTC
TCTGTATCGACGAGGTTTGA

Downstream 100 bases:

>100_bases
TTGATCATGAGTTTGGTTACACTATCCTCATAAACTAAAAAAGCTCCTAGATATGATGCTAGGAGCTTTTTTCATCAGGG
CTAAATCTTATTTACGCCCA

Product: amino acid carrier protein

Products: Na (I) [Cytoplasm]; L-alanine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MEAITNFVSMINGVVWGTPMLIMILGVGLLLTIGLRFMPILKLGTGFKLLWSGRIPDKDKNMKGEISPFNALMTSLSATI
GTGNIAGVATAIFIGGPGALFWMWCTALVGMATKFAEAVLAVKYREVDDNGNHVGGPMYYIKNGLGAKWAWLGTAFALFG
SLAGFGIGNTVQANSVADALSSNFGVPNWITGVVLMVLVGAVLMGGIKRIADVAGKLVPMMTIFYLAAGVAVLVVYASEI
PAAIELILHSAFNPVAAQGGFAGAAVWAAIRFGVARGVFSNEAGLGSAPIAHAAAQTNNPVAQGLVAMLGTFIDTLIVCS
ITGLAIIVSGAWTSGENGAALTSFAFSHAFPMGNYVVAIALSIFAFTTILGWSFYCEKCVQFLFGIKAIKPFRILWTIVV
PLGAVSSLEFIWLLADTLNAMMAIPNLIALALLSPVVFALTREYFLKKRQTDLESQKAK

Sequences:

>Translated_459_residues
MEAITNFVSMINGVVWGTPMLIMILGVGLLLTIGLRFMPILKLGTGFKLLWSGRIPDKDKNMKGEISPFNALMTSLSATI
GTGNIAGVATAIFIGGPGALFWMWCTALVGMATKFAEAVLAVKYREVDDNGNHVGGPMYYIKNGLGAKWAWLGTAFALFG
SLAGFGIGNTVQANSVADALSSNFGVPNWITGVVLMVLVGAVLMGGIKRIADVAGKLVPMMTIFYLAAGVAVLVVYASEI
PAAIELILHSAFNPVAAQGGFAGAAVWAAIRFGVARGVFSNEAGLGSAPIAHAAAQTNNPVAQGLVAMLGTFIDTLIVCS
ITGLAIIVSGAWTSGENGAALTSFAFSHAFPMGNYVVAIALSIFAFTTILGWSFYCEKCVQFLFGIKAIKPFRILWTIVV
PLGAVSSLEFIWLLADTLNAMMAIPNLIALALLSPVVFALTREYFLKKRQTDLESQKAK
>Mature_459_residues
MEAITNFVSMINGVVWGTPMLIMILGVGLLLTIGLRFMPILKLGTGFKLLWSGRIPDKDKNMKGEISPFNALMTSLSATI
GTGNIAGVATAIFIGGPGALFWMWCTALVGMATKFAEAVLAVKYREVDDNGNHVGGPMYYIKNGLGAKWAWLGTAFALFG
SLAGFGIGNTVQANSVADALSSNFGVPNWITGVVLMVLVGAVLMGGIKRIADVAGKLVPMMTIFYLAAGVAVLVVYASEI
PAAIELILHSAFNPVAAQGGFAGAAVWAAIRFGVARGVFSNEAGLGSAPIAHAAAQTNNPVAQGLVAMLGTFIDTLIVCS
ITGLAIIVSGAWTSGENGAALTSFAFSHAFPMGNYVVAIALSIFAFTTILGWSFYCEKCVQFLFGIKAIKPFRILWTIVV
PLGAVSSLEFIWLLADTLNAMMAIPNLIALALLSPVVFALTREYFLKKRQTDLESQKAK

Specific function: Unknown

COG id: COG1115

COG function: function code E; Na+/alanine symporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:alanine (SAF) symporter family [H]

Homologues:

Organism=Escherichia coli, GI1786188, Length=454, Percent_Identity=37.6651982378855, Blast_Score=245, Evalue=4e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001463 [H]

Pfam domain/function: PF01235 Na_Ala_symp [H]

EC number: NA

Molecular weight: Translated: 48321; Mature: 48321

Theoretical pI: Translated: 9.31; Mature: 9.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEAITNFVSMINGVVWGTPMLIMILGVGLLLTIGLRFMPILKLGTGFKLLWSGRIPDKDK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCC
NMKGEISPFNALMTSLSATIGTGNIAGVATAIFIGGPGALFWMWCTALVGMATKFAEAVL
CCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHH
AVKYREVDDNGNHVGGPMYYIKNGLGAKWAWLGTAFALFGSLAGFGIGNTVQANSVADAL
HHHHHEECCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
SSNFGVPNWITGVVLMVLVGAVLMGGIKRIADVAGKLVPMMTIFYLAAGVAVLVVYASEI
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PAAIELILHSAFNPVAAQGGFAGAAVWAAIRFGVARGVFSNEAGLGSAPIAHAAAQTNNP
HHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCH
VAQGLVAMLGTFIDTLIVCSITGLAIIVSGAWTSGENGAALTSFAFSHAFPMGNYVVAIA
HHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCHHHHHHHHHCCCCCHHHHHHH
LSIFAFTTILGWSFYCEKCVQFLFGIKAIKPFRILWTIVVPLGAVSSLEFIWLLADTLNA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MMAIPNLIALALLSPVVFALTREYFLKKRQTDLESQKAK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCC
>Mature Secondary Structure
MEAITNFVSMINGVVWGTPMLIMILGVGLLLTIGLRFMPILKLGTGFKLLWSGRIPDKDK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCC
NMKGEISPFNALMTSLSATIGTGNIAGVATAIFIGGPGALFWMWCTALVGMATKFAEAVL
CCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHH
AVKYREVDDNGNHVGGPMYYIKNGLGAKWAWLGTAFALFGSLAGFGIGNTVQANSVADAL
HHHHHEECCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
SSNFGVPNWITGVVLMVLVGAVLMGGIKRIADVAGKLVPMMTIFYLAAGVAVLVVYASEI
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PAAIELILHSAFNPVAAQGGFAGAAVWAAIRFGVARGVFSNEAGLGSAPIAHAAAQTNNP
HHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCH
VAQGLVAMLGTFIDTLIVCSITGLAIIVSGAWTSGENGAALTSFAFSHAFPMGNYVVAIA
HHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCHHHHHHHHHCCCCCHHHHHHH
LSIFAFTTILGWSFYCEKCVQFLFGIKAIKPFRILWTIVVPLGAVSSLEFIWLLADTLNA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MMAIPNLIALALLSPVVFALTREYFLKKRQTDLESQKAK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-alanine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-alanine [Periplasm] = Na (I) [Cytoplasm] + L-alanine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]