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The map label for this gene is groEL [H]

Identifier: 157963501

GI number: 157963501

Start: 4488521

End: 4490164

Strand: Reverse

Name: groEL [H]

Synonym: Spea_3689

Alternate gene names: 157963501

Gene position: 4490164-4488521 (Counterclockwise)

Preceding gene: 157963502

Following gene: 157963496

Centisome position: 86.77

GC content: 47.51

Gene sequence:

>1644_bases
ATGGCAGCTAAAGAAGTATTATTTGGTAATGACGCACGCGTAAAAATGCTAGCGGGCGTAAACATTCTTGCTAACGCAGT
TAAAGTAACTCTAGGCCCAAAAGGTCGTAACGTAGTACTAGACAAGAGCTTTGGTGCTCCGCTTATCACTAAAGATGGCG
TGTCAGTCGCTAAAGAGATCGAGCTTGAAGACAAGTTCGAAAACATGGGCGCACAGATGGTTAAAGAAGTTGCTTCTAAA
GCCAATGATGCAGCCGGTGACGGTACTACTACTGCTACAGTACTTGCGCAAGCAATCGTTACTGAAGGCCTAAAAGCTGT
TGCAGCGGGTATGAACCCAATGGATCTTAAGCGCGGTATCGACAAAGCGGTAGTTGCTGCTGTTGCCGAGCTTAAAAACC
TATCTCAAGAGTGTTCTGATACTAAAGCTATCGCTCAAGTAGGTACTATCTCTGCTAACTCTGACGAGTCAATTGGTGAA
ATCATCGCAACTGCGATGGAGCGCGTGGGTAAAGAAGGCGTTATCACAGTTGAAGAAGGTCAAGCACTAGAGAACGAGCT
AGACGTAGTTGAAGGTATGCAGTTCGACCGCGGTTACCTATCTCCATACTTCATCAACAAGCCAGAAACTGGCAGCGTTG
AACTAGAAAGCCCATTCATCCTTCTAGTAGACAAGAAAGTATCTAACATTCGTGAATTGCTACCAATCCTTGAAGGTCTA
GCTAAAACGGGTAAGCCACTGCTTATCGTTGCAGAAGACGTTGAAGGTGAAGCACTAGCAACACTAGTTGTGAACAACAT
GCGCGGTATCGTTAAAGTTGCAGCTGTTAAGGCGCCAGGCTTTGGTGACCGTCGTAAGGCAATGCTACAAGACATCGCTA
TCCTAACTGGCGGCACTGTTATTGCTGAAGAGATTGGTCTAGAGCTAGAAAAAGCGACTCTAGAAGATCTAGGTACGGCT
AAGCGCGTTATTATCACTAAAGATGACACGACTATCATCGACGGTAACGGCGAAGAGACTCAGATCAAAGCACGTGTTGC
ACAGATCAAAATTCAAGCAGAAGAGTCTACTTCAGACTACGACAAAGAGAAGCTTCAAGAGCGTATGGCTAAACTTGCTG
GCGGCGTAGCCGTAATCAAGGTTGGCGCAGCAACTGAAGTTGAAATGAAAGAGAAGAAAGCACGCGTAGAAGACGCACTA
CACGCAACTCGCGCAGCGGTTGAAGAAGGTGTGGTTGCTGGTGGTGGTGTTGCACTAGTACGCGTAGCAAGCAAAATCGG
TGAGCTAGACGTAATCAACGAAGACCAGAAGCACGGTGTGATCATCGCACTACGTGCAATGGAAGCGCCACTACGTCAAA
TCGCGGCTAACGCGGGTGAAGAAGGTTCAGTTGTTGCTAACAACGTTAAGAACGGCACCGGTAACTACGGTTACAACGCA
GGTAACGACACATACGGCGACATGCTAGAGATGGGTATCCTAGACCCAACTAAAGTAACTCGTAGCGCACTACAGTTCGC
TTCTTCAATTGCCGGCCTAATGATCACGACAGAGTGTATGGTTGCTGAAGTTAAAGAAGATGCAGCTGATATGGGCGGCA
TGGGCGGCATGGGTGGTATGGGCGGTATGGGCGGCATGATGTAA

Upstream 100 bases:

>100_bases
TAGTGGGTTAATCCCATACTCTTGCAACATACAAATCATTAATGCCGTTGTTGGCGCAACTGTGTAACCACGGCTAAAAA
GAATTGAAAGGATATTAAAG

Downstream 100 bases:

>100_bases
TTTTTGCTTTTTTGCTGGTCTAAATCGGTGATTACGGCGTTGCTGCTCGCTCGCGTAAGAAAACTACGCGTCGCTCACAG
CGCCTTGTACTAACACGATT

Product: chaperonin GroEL

Products: NA

Alternate protein names: GroEL protein; Protein Cpn60 [H]

Number of amino acids: Translated: 547; Mature: 546

Protein sequence:

>547_residues
MAAKEVLFGNDARVKMLAGVNILANAVKVTLGPKGRNVVLDKSFGAPLITKDGVSVAKEIELEDKFENMGAQMVKEVASK
ANDAAGDGTTTATVLAQAIVTEGLKAVAAGMNPMDLKRGIDKAVVAAVAELKNLSQECSDTKAIAQVGTISANSDESIGE
IIATAMERVGKEGVITVEEGQALENELDVVEGMQFDRGYLSPYFINKPETGSVELESPFILLVDKKVSNIRELLPILEGL
AKTGKPLLIVAEDVEGEALATLVVNNMRGIVKVAAVKAPGFGDRRKAMLQDIAILTGGTVIAEEIGLELEKATLEDLGTA
KRVIITKDDTTIIDGNGEETQIKARVAQIKIQAEESTSDYDKEKLQERMAKLAGGVAVIKVGAATEVEMKEKKARVEDAL
HATRAAVEEGVVAGGGVALVRVASKIGELDVINEDQKHGVIIALRAMEAPLRQIAANAGEEGSVVANNVKNGTGNYGYNA
GNDTYGDMLEMGILDPTKVTRSALQFASSIAGLMITTECMVAEVKEDAADMGGMGGMGGMGGMGGMM

Sequences:

>Translated_547_residues
MAAKEVLFGNDARVKMLAGVNILANAVKVTLGPKGRNVVLDKSFGAPLITKDGVSVAKEIELEDKFENMGAQMVKEVASK
ANDAAGDGTTTATVLAQAIVTEGLKAVAAGMNPMDLKRGIDKAVVAAVAELKNLSQECSDTKAIAQVGTISANSDESIGE
IIATAMERVGKEGVITVEEGQALENELDVVEGMQFDRGYLSPYFINKPETGSVELESPFILLVDKKVSNIRELLPILEGL
AKTGKPLLIVAEDVEGEALATLVVNNMRGIVKVAAVKAPGFGDRRKAMLQDIAILTGGTVIAEEIGLELEKATLEDLGTA
KRVIITKDDTTIIDGNGEETQIKARVAQIKIQAEESTSDYDKEKLQERMAKLAGGVAVIKVGAATEVEMKEKKARVEDAL
HATRAAVEEGVVAGGGVALVRVASKIGELDVINEDQKHGVIIALRAMEAPLRQIAANAGEEGSVVANNVKNGTGNYGYNA
GNDTYGDMLEMGILDPTKVTRSALQFASSIAGLMITTECMVAEVKEDAADMGGMGGMGGMGGMGGMM
>Mature_546_residues
AAKEVLFGNDARVKMLAGVNILANAVKVTLGPKGRNVVLDKSFGAPLITKDGVSVAKEIELEDKFENMGAQMVKEVASKA
NDAAGDGTTTATVLAQAIVTEGLKAVAAGMNPMDLKRGIDKAVVAAVAELKNLSQECSDTKAIAQVGTISANSDESIGEI
IATAMERVGKEGVITVEEGQALENELDVVEGMQFDRGYLSPYFINKPETGSVELESPFILLVDKKVSNIRELLPILEGLA
KTGKPLLIVAEDVEGEALATLVVNNMRGIVKVAAVKAPGFGDRRKAMLQDIAILTGGTVIAEEIGLELEKATLEDLGTAK
RVIITKDDTTIIDGNGEETQIKARVAQIKIQAEESTSDYDKEKLQERMAKLAGGVAVIKVGAATEVEMKEKKARVEDALH
ATRAAVEEGVVAGGGVALVRVASKIGELDVINEDQKHGVIIALRAMEAPLRQIAANAGEEGSVVANNVKNGTGNYGYNAG
NDTYGDMLEMGILDPTKVTRSALQFASSIAGLMITTECMVAEVKEDAADMGGMGGMGGMGGMGGMM

Specific function: Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions [H]

COG id: COG0459

COG function: function code O; Chaperonin GroEL (HSP60 family)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chaperonin (HSP60) family [H]

Homologues:

Organism=Homo sapiens, GI41399285, Length=529, Percent_Identity=50.6616257088847, Blast_Score=523, Evalue=1e-148,
Organism=Homo sapiens, GI31542947, Length=529, Percent_Identity=50.6616257088847, Blast_Score=523, Evalue=1e-148,
Organism=Escherichia coli, GI1790586, Length=532, Percent_Identity=83.2706766917293, Blast_Score=879, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17555558, Length=529, Percent_Identity=51.4177693761815, Blast_Score=533, Evalue=1e-152,
Organism=Caenorhabditis elegans, GI193210679, Length=211, Percent_Identity=52.132701421801, Blast_Score=208, Evalue=5e-54,
Organism=Saccharomyces cerevisiae, GI6323288, Length=526, Percent_Identity=54.5627376425855, Blast_Score=555, Evalue=1e-159,
Organism=Saccharomyces cerevisiae, GI6322524, Length=583, Percent_Identity=20.926243567753, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24641193, Length=527, Percent_Identity=52.561669829222, Blast_Score=546, Evalue=1e-155,
Organism=Drosophila melanogaster, GI24641191, Length=527, Percent_Identity=52.561669829222, Blast_Score=546, Evalue=1e-155,
Organism=Drosophila melanogaster, GI45550936, Length=523, Percent_Identity=48.7571701720841, Blast_Score=508, Evalue=1e-144,
Organism=Drosophila melanogaster, GI45550132, Length=523, Percent_Identity=48.7571701720841, Blast_Score=508, Evalue=1e-144,
Organism=Drosophila melanogaster, GI45550935, Length=523, Percent_Identity=48.7571701720841, Blast_Score=508, Evalue=1e-144,
Organism=Drosophila melanogaster, GI17864606, Length=540, Percent_Identity=41.6666666666667, Blast_Score=438, Evalue=1e-123,
Organism=Drosophila melanogaster, GI24584129, Length=548, Percent_Identity=35.7664233576642, Blast_Score=321, Evalue=8e-88,
Organism=Drosophila melanogaster, GI19921262, Length=548, Percent_Identity=35.7664233576642, Blast_Score=321, Evalue=8e-88,

Paralogues:

None

Copy number: 2180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 480 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 15012 Molecules/Cell In: Growth Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018370
- InterPro:   IPR001844
- InterPro:   IPR002423 [H]

Pfam domain/function: PF00118 Cpn60_TCP1 [H]

EC number: NA

Molecular weight: Translated: 57288; Mature: 57156

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS00296 CHAPERONINS_CPN60

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAKEVLFGNDARVKMLAGVNILANAVKVTLGPKGRNVVLDKSFGAPLITKDGVSVAKEI
CCCCCEEECCCCCEEEEECHHHHHHHEEEEECCCCCEEEEECCCCCCEEECCCCHHHHHC
ELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIVTEGLKAVAAGMNPMDLKRGI
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCH
DKAVVAAVAELKNLSQECSDTKAIAQVGTISANSDESIGEIIATAMERVGKEGVITVEEG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCC
QALENELDVVEGMQFDRGYLSPYFINKPETGSVELESPFILLVDKKVSNIRELLPILEGL
CHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCEEECCCEEEEECCHHHHHHHHHHHHHHH
AKTGKPLLIVAEDVEGEALATLVVNNMRGIVKVAAVKAPGFGDRRKAMLQDIAILTGGTV
HHCCCCEEEEEECCCCCHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCH
IAEEIGLELEKATLEDLGTAKRVIITKDDTTIIDGNGEETQIKARVAQIKIQAEESTSDY
HHHHHCCHHHHHHHHHCCCCCEEEEECCCCEEECCCCCCHHHEEEEEEEEEEECCCCCCC
DKEKLQERMAKLAGGVAVIKVGAATEVEMKEKKARVEDALHATRAAVEEGVVAGGGVALV
HHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
RVASKIGELDVINEDQKHGVIIALRAMEAPLRQIAANAGEEGSVVANNVKNGTGNYGYNA
HHHHHHCCEEECCCCCCCCEEEEEEHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCC
GNDTYGDMLEMGILDPTKVTRSALQFASSIAGLMITTECMVAEVKEDAADMGGMGGMGGM
CCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
GGMGGMM
CCCCCCC
>Mature Secondary Structure 
AAKEVLFGNDARVKMLAGVNILANAVKVTLGPKGRNVVLDKSFGAPLITKDGVSVAKEI
CCCCEEECCCCCEEEEECHHHHHHHEEEEECCCCCEEEEECCCCCCEEECCCCHHHHHC
ELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIVTEGLKAVAAGMNPMDLKRGI
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCH
DKAVVAAVAELKNLSQECSDTKAIAQVGTISANSDESIGEIIATAMERVGKEGVITVEEG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCC
QALENELDVVEGMQFDRGYLSPYFINKPETGSVELESPFILLVDKKVSNIRELLPILEGL
CHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCEEECCCEEEEECCHHHHHHHHHHHHHHH
AKTGKPLLIVAEDVEGEALATLVVNNMRGIVKVAAVKAPGFGDRRKAMLQDIAILTGGTV
HHCCCCEEEEEECCCCCHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCH
IAEEIGLELEKATLEDLGTAKRVIITKDDTTIIDGNGEETQIKARVAQIKIQAEESTSDY
HHHHHCCHHHHHHHHHCCCCCEEEEECCCCEEECCCCCCHHHEEEEEEEEEEECCCCCCC
DKEKLQERMAKLAGGVAVIKVGAATEVEMKEKKARVEDALHATRAAVEEGVVAGGGVALV
HHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
RVASKIGELDVINEDQKHGVIIALRAMEAPLRQIAANAGEEGSVVANNVKNGTGNYGYNA
HHHHHHCCEEECCCCCCCCEEEEEEHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCC
GNDTYGDMLEMGILDPTKVTRSALQFASSIAGLMITTECMVAEVKEDAADMGGMGGMGGM
CCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
GGMGGMM
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA