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The map label for this gene is appB [H]

Identifier: 157963455

GI number: 157963455

Start: 4430832

End: 4431809

Strand: Reverse

Name: appB [H]

Synonym: Spea_3643

Alternate gene names: 157963455

Gene position: 4431809-4430832 (Counterclockwise)

Preceding gene: 157963456

Following gene: 157963454

Centisome position: 85.65

GC content: 49.28

Gene sequence:

>978_bases
ATGAGCAACTTATCCTCCTCGATATTGCACACCGGCCTGCGTTTGCTGTTGCTGCTCTTTCTCGTTGTCGTGTTTGCCTA
TGTTTTGCTAAGTTGGTCGCCACTGGATCCGATTAATGCCTATCTTGGCGGCAATGTTTTTGCCGTCTCAGAAGCGCAAA
AATCCCTGTTAGTGAGCCAGTTGGCGTTGGATCAAAGTGTCGGTGAGCGTTTGATGCATTGGCTTTCGGGTTTAATGCAG
GGGGAGCTTGGTTACTCAAGCCTCTATCAGCAACCCGTGTCGAGTGTGATTGCAGAGCAGTTACCGTTATCACTGCTATT
GATTGGCTTAAGCTGGATCTGCTCATTAGTTTTCGGCTATCTGCTTGGCTTAGTCGCGGCTATTTATCGCGGCGGTTGGT
TAGATCGCATCATTCAAAAGCTGGCATGGTTGATGGTCTGTATGCCGTCGTTCTGGCTAGCTATGCTGCTTATCAGCCTA
TTTTCAATCGGCCTTAGCCTGACACCCGTTTGCTGTGCCGCCCCGATCGGCATGACCTTTATGGATCAGTCGTTAGGCAG
CATGGTGCTACACCTGATGTTGCCCGTCGCGACATTGACCCTTGTGAGCATGGCGCCGATCATGCTGCATACTCGGGAGA
AGGTGATCGATGTATTGCAGTCAGATTATGTGCAGTACTCCTTAGCCCATGGCGATAGCTTATCTAGAGTGGTGTTGTTT
CATGTGATCCGCAATAGTCTGCTGCCTGCGATTATCTTGCAGTTTGCCACCATCGCTGAGTTGTTTGGCGGCTCCTTGAT
TGCCGAGACGGTATTTAGCTTTCCAGGGCTCGGGGCTGGTATTGTGAAAGCGGGATTGGCTAGCGATACGGCTTTATTAA
TGGGCTGCACGTTAATATCTGCGCTATTGGTATTTAGTGGCAACCTGATCGCTAACCTACTGTCAGGTTATTTTATGCTA
GAGGCTAATGATGTATAA

Upstream 100 bases:

>100_bases
ATGAATGCCTAGATCTTGCCAAACCAGGCATCGAGGCCCACGGACATGGTTGGCCGCTTGCCAATAATATCGAGCAGTGG
CGTTGGCAGTGTCCGAGTCG

Downstream 100 bases:

>100_bases
AGCCCTACGCCAACCGAGTTTTATCTTGGCTAGTCTCAGCTTGTTAAGCTTAGTGATATTAGCCTTTTTCTGGCCTTGGT
TGGGGGATGAACTCAACCTT

Product: binding-protein-dependent transport systems inner membrane component

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MSNLSSSILHTGLRLLLLLFLVVVFAYVLLSWSPLDPINAYLGGNVFAVSEAQKSLLVSQLALDQSVGERLMHWLSGLMQ
GELGYSSLYQQPVSSVIAEQLPLSLLLIGLSWICSLVFGYLLGLVAAIYRGGWLDRIIQKLAWLMVCMPSFWLAMLLISL
FSIGLSLTPVCCAAPIGMTFMDQSLGSMVLHLMLPVATLTLVSMAPIMLHTREKVIDVLQSDYVQYSLAHGDSLSRVVLF
HVIRNSLLPAIILQFATIAELFGGSLIAETVFSFPGLGAGIVKAGLASDTALLMGCTLISALLVFSGNLIANLLSGYFML
EANDV

Sequences:

>Translated_325_residues
MSNLSSSILHTGLRLLLLLFLVVVFAYVLLSWSPLDPINAYLGGNVFAVSEAQKSLLVSQLALDQSVGERLMHWLSGLMQ
GELGYSSLYQQPVSSVIAEQLPLSLLLIGLSWICSLVFGYLLGLVAAIYRGGWLDRIIQKLAWLMVCMPSFWLAMLLISL
FSIGLSLTPVCCAAPIGMTFMDQSLGSMVLHLMLPVATLTLVSMAPIMLHTREKVIDVLQSDYVQYSLAHGDSLSRVVLF
HVIRNSLLPAIILQFATIAELFGGSLIAETVFSFPGLGAGIVKAGLASDTALLMGCTLISALLVFSGNLIANLLSGYFML
EANDV
>Mature_324_residues
SNLSSSILHTGLRLLLLLFLVVVFAYVLLSWSPLDPINAYLGGNVFAVSEAQKSLLVSQLALDQSVGERLMHWLSGLMQG
ELGYSSLYQQPVSSVIAEQLPLSLLLIGLSWICSLVFGYLLGLVAAIYRGGWLDRIIQKLAWLMVCMPSFWLAMLLISLF
SIGLSLTPVCCAAPIGMTFMDQSLGSMVLHLMLPVATLTLVSMAPIMLHTREKVIDVLQSDYVQYSLAHGDSLSRVVLFH
VIRNSLLPAIILQFATIAELFGGSLIAETVFSFPGLGAGIVKAGLASDTALLMGCTLISALLVFSGNLIANLLSGYFMLE
ANDV

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence, though, unlike OPP, is incapable of transportin

COG id: COG0601

COG function: function code EP; ABC-type dipeptide/oligopeptide/nickel transport systems, permease components

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789965, Length=315, Percent_Identity=30.1587301587302, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1787053, Length=303, Percent_Identity=31.023102310231, Blast_Score=131, Evalue=6e-32,
Organism=Escherichia coli, GI1787550, Length=262, Percent_Identity=30.1526717557252, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1789888, Length=306, Percent_Identity=28.4313725490196, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1787761, Length=301, Percent_Identity=27.2425249169435, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1787497, Length=310, Percent_Identity=26.1290322580645, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788504, Length=262, Percent_Identity=25.1908396946565, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 35087; Mature: 34956

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHCCHHHHHHHCCHHHEECCCC
>Mature Secondary Structure 
SNLSSSILHTGLRLLLLLFLVVVFAYVLLSWSPLDPINAYLGGNVFAVSEAQKSLLVSQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCEEEHHHHHHHHHHHH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HHHHHCCHHHHHHHCCHHHEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]