Click here to switch to the map view.

The map label for this gene is amiB [H]

Identifier: 157963357

GI number: 157963357

Start: 4326832

End: 4328154

Strand: Reverse

Name: amiB [H]

Synonym: Spea_3543

Alternate gene names: 157963357

Gene position: 4328154-4326832 (Counterclockwise)

Preceding gene: 157963358

Following gene: 157963356

Centisome position: 83.64

GC content: 42.55

Gene sequence:

>1323_bases
ATGATAATAAACAGTAATATATTTAGATTATTTCTACTTCTTGTTACGTTTGGTGCTTCATTCTTTGCGCAGAGTGCGAA
TAAACTCGAAAGCGTAAGGATTTGGGCTGCGCCAGACTCGACTCGTCTTGTTTTCGATTTGAGTCAGTCTCCAGACTATA
GCTATTTCACTCTTACTAATCCTTATCGATTAGTGGTGGATTTGAAGACCTCCTCCAATAGAGTCGATCTCAATAAGATA
AAAAATAACAGTAAGCTGGTTAAGAAGGTACGTAAGAGTACCCCTCCAGAGAAGGGCACGTTGCGGTTGGTTATCGATCT
AGTTAAGCCAGTCAAGGCGAGTTTGTTTGCATTGCCTCCAACCGCACCATACGGGAATCGTTTGGTGGTTGATTTAGATG
GTGCAGGATCGACAGCGAAAAAGCCTACTGTCACTAAACCAAAAACTCAATTAAGAGATGTGGTGATTGCCATTGATGCG
GGTCATGGTGGAGAAGATCCCGGTTCTATCGGACCGACTGGTATCTATGAGAAAAAACTAGTATTAGAGATATCTAAAAA
AGTCGCTCAAAAAATTAATGCCACACCGGGTATGAGGGCTGTAATGACGCGAACAGGCGATTATTTTGTTAATTTGAATA
AACGCTCTGACATCGCCCGCAATAGTAAGGCTGATCTTCTCATTTCTATCCATGCGGATGCCTTTACTTCACCCCAGCCT
AGGGGGGCATCGGTATGGGTATTATCCATGCGCCGTGCAAACAGTGAAATTGGTCGCTGGTTAGAGCAAAAAGAAAAACA
TTCCGAACTACTTGGTGGTGCGGGAGAGATAATTCAAAATACCGATAATGAACAGTATCTTGCGATGACATTGTTAGATA
TGTCTATGGATCGCTCTATGGCGATTAGCCATAATATTGCTGGTGATGTGCTCTCCAACCTTGGACGCGTGACTAAACTG
CATAAGCATAAACCTGAATCAGCCAGTTTTGCCGTACTCAAGTCCCCAGATATTCCATCTATTTTGGTAGAAACTGGCTT
TATCTCAAACCCAAAAGAAGAGCGACTATTATCACAGCGTGATCATCAGAATAATGTCGCTAAGGCGATCCACAAAGGTG
TGGTTAAGTATTTTGAGGCTAACCCTCCAGTCAACTCATTACTAGCGAGTAAGAGCAGTGTTGAGCATAAGGTTCGAAGT
GGAGAGTCGTTATCGGTTATTGCGCATCGTTACCAGATCTCGATAAGCCGTTTGAAGAAGGCAAACAACCTTAAATCAAA
TAACCTACGAATTGGTCAGAAATTAAAGATTCCGAGTGCGTAA

Upstream 100 bases:

>100_bases
GATGTACATATCCACATTGAATATGTTAATACTGGAAGGCAAGTTGAGTTACAGGCACTTTCTCCAAAAGGGGAACAAAT
CCTAGCGGCCCTAAATAATA

Downstream 100 bases:

>100_bases
GGGCTTTTGAGAGTACACAATGGCAATAGAAAAATTACCGCCACAATTAGCTAATCAGATCGCCGCAGGTGAGGTGGTCG
AACGTCCTGCATCTGTGATT

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MIINSNIFRLFLLLVTFGASFFAQSANKLESVRIWAAPDSTRLVFDLSQSPDYSYFTLTNPYRLVVDLKTSSNRVDLNKI
KNNSKLVKKVRKSTPPEKGTLRLVIDLVKPVKASLFALPPTAPYGNRLVVDLDGAGSTAKKPTVTKPKTQLRDVVIAIDA
GHGGEDPGSIGPTGIYEKKLVLEISKKVAQKINATPGMRAVMTRTGDYFVNLNKRSDIARNSKADLLISIHADAFTSPQP
RGASVWVLSMRRANSEIGRWLEQKEKHSELLGGAGEIIQNTDNEQYLAMTLLDMSMDRSMAISHNIAGDVLSNLGRVTKL
HKHKPESASFAVLKSPDIPSILVETGFISNPKEERLLSQRDHQNNVAKAIHKGVVKYFEANPPVNSLLASKSSVEHKVRS
GESLSVIAHRYQISISRLKKANNLKSNNLRIGQKLKIPSA

Sequences:

>Translated_440_residues
MIINSNIFRLFLLLVTFGASFFAQSANKLESVRIWAAPDSTRLVFDLSQSPDYSYFTLTNPYRLVVDLKTSSNRVDLNKI
KNNSKLVKKVRKSTPPEKGTLRLVIDLVKPVKASLFALPPTAPYGNRLVVDLDGAGSTAKKPTVTKPKTQLRDVVIAIDA
GHGGEDPGSIGPTGIYEKKLVLEISKKVAQKINATPGMRAVMTRTGDYFVNLNKRSDIARNSKADLLISIHADAFTSPQP
RGASVWVLSMRRANSEIGRWLEQKEKHSELLGGAGEIIQNTDNEQYLAMTLLDMSMDRSMAISHNIAGDVLSNLGRVTKL
HKHKPESASFAVLKSPDIPSILVETGFISNPKEERLLSQRDHQNNVAKAIHKGVVKYFEANPPVNSLLASKSSVEHKVRS
GESLSVIAHRYQISISRLKKANNLKSNNLRIGQKLKIPSA
>Mature_440_residues
MIINSNIFRLFLLLVTFGASFFAQSANKLESVRIWAAPDSTRLVFDLSQSPDYSYFTLTNPYRLVVDLKTSSNRVDLNKI
KNNSKLVKKVRKSTPPEKGTLRLVIDLVKPVKASLFALPPTAPYGNRLVVDLDGAGSTAKKPTVTKPKTQLRDVVIAIDA
GHGGEDPGSIGPTGIYEKKLVLEISKKVAQKINATPGMRAVMTRTGDYFVNLNKRSDIARNSKADLLISIHADAFTSPQP
RGASVWVLSMRRANSEIGRWLEQKEKHSELLGGAGEIIQNTDNEQYLAMTLLDMSMDRSMAISHNIAGDVLSNLGRVTKL
HKHKPESASFAVLKSPDIPSILVETGFISNPKEERLLSQRDHQNNVAKAIHKGVVKYFEANPPVNSLLASKSSVEHKVRS
GESLSVIAHRYQISISRLKKANNLKSNNLRIGQKLKIPSA

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1790611, Length=416, Percent_Identity=37.2596153846154, Blast_Score=276, Evalue=2e-75,
Organism=Escherichia coli, GI87082163, Length=387, Percent_Identity=37.2093023255814, Blast_Score=231, Evalue=6e-62,
Organism=Escherichia coli, GI1788776, Length=225, Percent_Identity=41.7777777777778, Blast_Score=173, Evalue=2e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508 [H]

Pfam domain/function: PF01520 Amidase_3 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 48463; Mature: 48463

Theoretical pI: Translated: 10.67; Mature: 10.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIINSNIFRLFLLLVTFGASFFAQSANKLESVRIWAAPDSTRLVFDLSQSPDYSYFTLTN
CEECCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCEEEEEECCCCCCCEEEECC
PYRLVVDLKTSSNRVDLNKIKNNSKLVKKVRKSTPPEKGTLRLVIDLVKPVKASLFALPP
CEEEEEEEECCCCEEEHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHEEECCC
TAPYGNRLVVDLDGAGSTAKKPTVTKPKTQLRDVVIAIDAGHGGEDPGSIGPTGIYEKKL
CCCCCCEEEEEECCCCCCCCCCCCCCCHHHHCEEEEEEECCCCCCCCCCCCCCCCHHHHH
VLEISKKVAQKINATPGMRAVMTRTGDYFVNLNKRSDIARNSKADLLISIHADAFTSPQP
HHHHHHHHHHHHCCCCCCEEEEEECCCEEEEECCCCCCCCCCCCCEEEEEECCCCCCCCC
RGASVWVLSMRRANSEIGRWLEQKEKHSELLGGAGEIIQNTDNEQYLAMTLLDMSMDRSM
CCCEEEEEEEHHCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCEEEEEEHHHHCCCCH
AISHNIAGDVLSNLGRVTKLHKHKPESASFAVLKSPDIPSILVETGFISNPKEERLLSQR
HHHHCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEEECCCCCCCHHHHHHHHH
DHQNNVAKAIHKGVVKYFEANPPVNSLLASKSSVEHKVRSGESLSVIAHRYQISISRLKK
CCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHH
ANNLKSNNLRIGQKLKIPSA
HCCCCCCCEECCCEECCCCC
>Mature Secondary Structure
MIINSNIFRLFLLLVTFGASFFAQSANKLESVRIWAAPDSTRLVFDLSQSPDYSYFTLTN
CEECCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCEEEEEECCCCCCCEEEECC
PYRLVVDLKTSSNRVDLNKIKNNSKLVKKVRKSTPPEKGTLRLVIDLVKPVKASLFALPP
CEEEEEEEECCCCEEEHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHEEECCC
TAPYGNRLVVDLDGAGSTAKKPTVTKPKTQLRDVVIAIDAGHGGEDPGSIGPTGIYEKKL
CCCCCCEEEEEECCCCCCCCCCCCCCCHHHHCEEEEEEECCCCCCCCCCCCCCCCHHHHH
VLEISKKVAQKINATPGMRAVMTRTGDYFVNLNKRSDIARNSKADLLISIHADAFTSPQP
HHHHHHHHHHHHCCCCCCEEEEEECCCEEEEECCCCCCCCCCCCCEEEEEECCCCCCCCC
RGASVWVLSMRRANSEIGRWLEQKEKHSELLGGAGEIIQNTDNEQYLAMTLLDMSMDRSM
CCCEEEEEEEHHCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCEEEEEEHHHHCCCCH
AISHNIAGDVLSNLGRVTKLHKHKPESASFAVLKSPDIPSILVETGFISNPKEERLLSQR
HHHHCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEEECCCCCCCHHHHHHHHH
DHQNNVAKAIHKGVVKYFEANPPVNSLLASKSSVEHKVRSGESLSVIAHRYQISISRLKK
CCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHH
ANNLKSNNLRIGQKLKIPSA
HCCCCCCCEECCCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609; 2676972 [H]