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The map label for this gene is miaA [H]

Identifier: 157963355

GI number: 157963355

Start: 4324010

End: 4324900

Strand: Reverse

Name: miaA [H]

Synonym: Spea_3541

Alternate gene names: 157963355

Gene position: 4324900-4324010 (Counterclockwise)

Preceding gene: 157963356

Following gene: 157963354

Centisome position: 83.58

GC content: 47.14

Gene sequence:

>891_bases
ATGGGGCCTACAGCCTCAGGTAAAACCGCATTAGCGATTGAGCTTGTTAAGCAGTATGACTGTGAGATCATCTCTGTGGA
CTCAGCATTGATCTACAAAGATATGGATATCGGTACCGCTAAGCCAGATGCAGCTGAGCAAGCGGCAGCGCCTCACCGAC
TGATTGATATTATTGATCCCGCGCAGAGCTACTCTGCGGCGGACTTTAGACGTGACGCCTTGGCTCAGATTGAAGATATT
TTGAGTCGTGGTAAGACGCCGTTACTCGTCGGTGGCACCATGATGTATTTCAAGGCATTGTTAGAAGGCTTATCGCCGCT
ACCTGGTGCAGATGAAGCGATAAGGGAGCAGATTGCAGCTGAAGCCGAAGCGCAAGGTTGGCAAGCTCTGCATGATCAGT
TGCGCGAAATTGATCCCGTTTCTGCTGAGCGAATTCACCCGAATGATCCGCAGCGACTAGCGAGAGCTTTGGAGGTATAT
CGAGTTAGTGGTCAAACTCTCACTGAGCTGACTAAGACCAAATCTGACGTATTCCCTTACGATGTGGTGCAATTTGCGAT
TGCACCAAGTGACAGAAAGGTGTTACATAGGGCTATAGAGACTCGATTTAAAGCCATGCTAGCACAAGGGTTTACTAGCG
AAGTTGAGCGTTTAAAGGCTCGAACGGACTTGAATTTAGACCTGCCATCTATGCGTTGTGTAGGATATAGGCAGTGCTGG
CAGTATTTAGATGGTGAAATTGACTATGATACTATGGTTGAAAAAGCTATTGTTGCTACTAGGCAATTAGCTAAACGTCA
ATTAACATGGTTACGCGGTTGGCCCGATTTAATCTGGCTAGAAAGCGGGGCTGAAAATAATCTAGCAACAGTAATGCGAC
ATAGTCGCTAG

Upstream 100 bases:

>100_bases
CAGTGTGAGCTTTATAATCACTCCCAAGAGTTACCTTGGGAGCAATGGATGAAAGAAAATCAGAGTGACCGATAAAAAAT
CACAGAAAGTTATTACCTTG

Downstream 100 bases:

>100_bases
CTTAATAGCTGCTGCTGTATAATACTAAAACCAAAGATAAAGTTTTATTGTATTAAAATAACTATTAATTTTAATTAAAA
AGGAAATTAGAAAATGGCAA

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase [H]

Number of amino acids: Translated: 296; Mature: 295

Protein sequence:

>296_residues
MGPTASGKTALAIELVKQYDCEIISVDSALIYKDMDIGTAKPDAAEQAAAPHRLIDIIDPAQSYSAADFRRDALAQIEDI
LSRGKTPLLVGGTMMYFKALLEGLSPLPGADEAIREQIAAEAEAQGWQALHDQLREIDPVSAERIHPNDPQRLARALEVY
RVSGQTLTELTKTKSDVFPYDVVQFAIAPSDRKVLHRAIETRFKAMLAQGFTSEVERLKARTDLNLDLPSMRCVGYRQCW
QYLDGEIDYDTMVEKAIVATRQLAKRQLTWLRGWPDLIWLESGAENNLATVMRHSR

Sequences:

>Translated_296_residues
MGPTASGKTALAIELVKQYDCEIISVDSALIYKDMDIGTAKPDAAEQAAAPHRLIDIIDPAQSYSAADFRRDALAQIEDI
LSRGKTPLLVGGTMMYFKALLEGLSPLPGADEAIREQIAAEAEAQGWQALHDQLREIDPVSAERIHPNDPQRLARALEVY
RVSGQTLTELTKTKSDVFPYDVVQFAIAPSDRKVLHRAIETRFKAMLAQGFTSEVERLKARTDLNLDLPSMRCVGYRQCW
QYLDGEIDYDTMVEKAIVATRQLAKRQLTWLRGWPDLIWLESGAENNLATVMRHSR
>Mature_295_residues
GPTASGKTALAIELVKQYDCEIISVDSALIYKDMDIGTAKPDAAEQAAAPHRLIDIIDPAQSYSAADFRRDALAQIEDIL
SRGKTPLLVGGTMMYFKALLEGLSPLPGADEAIREQIAAEAEAQGWQALHDQLREIDPVSAERIHPNDPQRLARALEVYR
VSGQTLTELTKTKSDVFPYDVVQFAIAPSDRKVLHRAIETRFKAMLAQGFTSEVERLKARTDLNLDLPSMRCVGYRQCWQ
YLDGEIDYDTMVEKAIVATRQLAKRQLTWLRGWPDLIWLESGAENNLATVMRHSR

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A) [H]

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family [H]

Homologues:

Organism=Homo sapiens, GI31581534, Length=305, Percent_Identity=29.5081967213115, Blast_Score=125, Evalue=5e-29,
Organism=Escherichia coli, GI1790613, Length=282, Percent_Identity=68.4397163120567, Blast_Score=403, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI25144712, Length=270, Percent_Identity=30, Blast_Score=103, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6324848, Length=282, Percent_Identity=28.0141843971631, Blast_Score=133, Evalue=4e-32,
Organism=Drosophila melanogaster, GI24649830, Length=280, Percent_Identity=32.1428571428571, Blast_Score=135, Evalue=4e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002627
- InterPro:   IPR018022 [H]

Pfam domain/function: PF01715 IPPT [H]

EC number: =2.5.1.75 [H]

Molecular weight: Translated: 33098; Mature: 32967

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGPTASGKTALAIELVKQYDCEIISVDSALIYKDMDIGTAKPDAAEQAAAPHRLIDIIDP
CCCCCCCCHHHHHHHHHHHCCCEEEECCCCEEECCCCCCCCCCHHHHHCCCHHHHHHHCC
AQSYSAADFRRDALAQIEDILSRGKTPLLVGGTMMYFKALLEGLSPLPGADEAIREQIAA
HHHCCHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
EAEAQGWQALHDQLREIDPVSAERIHPNDPQRLARALEVYRVSGQTLTELTKTKSDVFPY
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCH
DVVQFAIAPSDRKVLHRAIETRFKAMLAQGFTSEVERLKARTDLNLDLPSMRCVGYRQCW
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
QYLDGEIDYDTMVEKAIVATRQLAKRQLTWLRGWPDLIWLESGAENNLATVMRHSR
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHCCC
>Mature Secondary Structure 
GPTASGKTALAIELVKQYDCEIISVDSALIYKDMDIGTAKPDAAEQAAAPHRLIDIIDP
CCCCCCCHHHHHHHHHHHCCCEEEECCCCEEECCCCCCCCCCHHHHHCCCHHHHHHHCC
AQSYSAADFRRDALAQIEDILSRGKTPLLVGGTMMYFKALLEGLSPLPGADEAIREQIAA
HHHCCHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
EAEAQGWQALHDQLREIDPVSAERIHPNDPQRLARALEVYRVSGQTLTELTKTKSDVFPY
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCH
DVVQFAIAPSDRKVLHRAIETRFKAMLAQGFTSEVERLKARTDLNLDLPSMRCVGYRQCW
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
QYLDGEIDYDTMVEKAIVATRQLAKRQLTWLRGWPDLIWLESGAENNLATVMRHSR
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA