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The map label for this gene is lit [H]

Identifier: 157963268

GI number: 157963268

Start: 4222148

End: 4222978

Strand: Reverse

Name: lit [H]

Synonym: Spea_3454

Alternate gene names: 157963268

Gene position: 4222978-4222148 (Counterclockwise)

Preceding gene: 157963269

Following gene: 157963267

Centisome position: 81.61

GC content: 35.02

Gene sequence:

>831_bases
ATGTCAGCAGTTGCATCGCGAGTTGCACCTGAGAATGAAAGTAAGCTAGTGGAGTTATCTAACAAGCATGACTTTTCGCT
ACTTTTTTTGAAAGAGTCAGGCTTTACTTTTCGTGTTAACACAAAAACAAAGGTTATAAAGATCCCTTATGGCGGCTTGG
AGTATTTATGGACGTTTAGTTTGAAAGCTTGGTTACTTTATCAAGCATACGCTCAGGCACAATTGAATGGTGAACAAAAC
TTAGACTTGGAAACTATTAATGGTTATGCTGGAACAAGTCAGTTAACTGAATATTTGAAAGAAAGTCTATATGCGGAAAG
TTATGACCCAAATAAAAACTTTAGCGCTTTAATAAGTGTTGAGGAAATTGATCAGGAGGTAGCAACGGAAATATTCCTTT
GTGCTTTAGGATGGATTATTTGGCATGAAATTGCACATATTGAGTTAGGTCATAGTTCTCTAGAAATTAATACCTTGTCA
ATACAAGAAGAAAAAGATGCTGACTTATATAGTACCAATTGGATACTAAGTTCTTCTACTATAGCAGCAGAATCAAAAAA
ACGTATTGTTGGTATAACTATCGCATTGTTAGCAATTCAGAGCTTAGAGCTAGATTCTAAATCTTGCTTTAAAGGTACTC
ATCCTGATGCGAGTAATCGTATTTTTGATAACTTATCACAACACTCTGAAATAGGTGATGAAATCAGTCAAGCAATGAGT
ATCGTGACGTTACAATCTATGACTAAAATAGACATAGCTCCTATGAGCGACCTGAACTTCTCAGATATGTTAGGAGAAGC
TTTGTATCAAATAAATGTAAATAAGCGTTAA

Upstream 100 bases:

>100_bases
AGGTTAAGTGGTGGCTTGCTCACACCTTAACGCGGCGTTAGCAGAATTAGCATAGTTCAGAGGAAGCAATACATTGTTTA
CAAGAGAGCATTTTGAATTA

Downstream 100 bases:

>100_bases
AATTCAGCTAACAAGCATTTAAGAGTGACTCCCAAAGCTTGGCATTTTTAATTCCATCGTTGGGTTTCGTGTTTAAAGTG
GTATGGTTAAGTTCAGTGGT

Product: phage exclusion protein Lit

Products: NA

Alternate protein names: GpLit [H]

Number of amino acids: Translated: 276; Mature: 275

Protein sequence:

>276_residues
MSAVASRVAPENESKLVELSNKHDFSLLFLKESGFTFRVNTKTKVIKIPYGGLEYLWTFSLKAWLLYQAYAQAQLNGEQN
LDLETINGYAGTSQLTEYLKESLYAESYDPNKNFSALISVEEIDQEVATEIFLCALGWIIWHEIAHIELGHSSLEINTLS
IQEEKDADLYSTNWILSSSTIAAESKKRIVGITIALLAIQSLELDSKSCFKGTHPDASNRIFDNLSQHSEIGDEISQAMS
IVTLQSMTKIDIAPMSDLNFSDMLGEALYQINVNKR

Sequences:

>Translated_276_residues
MSAVASRVAPENESKLVELSNKHDFSLLFLKESGFTFRVNTKTKVIKIPYGGLEYLWTFSLKAWLLYQAYAQAQLNGEQN
LDLETINGYAGTSQLTEYLKESLYAESYDPNKNFSALISVEEIDQEVATEIFLCALGWIIWHEIAHIELGHSSLEINTLS
IQEEKDADLYSTNWILSSSTIAAESKKRIVGITIALLAIQSLELDSKSCFKGTHPDASNRIFDNLSQHSEIGDEISQAMS
IVTLQSMTKIDIAPMSDLNFSDMLGEALYQINVNKR
>Mature_275_residues
SAVASRVAPENESKLVELSNKHDFSLLFLKESGFTFRVNTKTKVIKIPYGGLEYLWTFSLKAWLLYQAYAQAQLNGEQNL
DLETINGYAGTSQLTEYLKESLYAESYDPNKNFSALISVEEIDQEVATEIFLCALGWIIWHEIAHIELGHSSLEINTLSI
QEEKDADLYSTNWILSSSTIAAESKKRIVGITIALLAIQSLELDSKSCFKGTHPDASNRIFDNLSQHSEIGDEISQAMSI
VTLQSMTKIDIAPMSDLNFSDMLGEALYQINVNKR

Specific function: Interacts with a short DNA sequence about one-quarter of the way into the major capsid protein gene 23 of T4, and the inhibition occurs when this late gene of the virus is expressed [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U49 family [H]

Homologues:

Organism=Escherichia coli, GI1787385, Length=278, Percent_Identity=34.5323741007194, Blast_Score=145, Evalue=4e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019504 [H]

Pfam domain/function: PF10463 Peptidase_U49 [H]

EC number: NA

Molecular weight: Translated: 30918; Mature: 30787

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAVASRVAPENESKLVELSNKHDFSLLFLKESGFTFRVNTKTKVIKIPYGGLEYLWTFS
CCCHHHHCCCCCCCCEEEECCCCCEEEEEEECCCCEEEEECCEEEEEECCCCHHHHHHHH
LKAWLLYQAYAQAQLNGEQNLDLETINGYAGTSQLTEYLKESLYAESYDPNKNFSALISV
HHHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEH
EEIDQEVATEIFLCALGWIIWHEIAHIELGHSSLEINTLSIQEEKDADLYSTNWILSSST
HHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEEEEEEECCCCCCCEEECCEEEECCE
IAAESKKRIVGITIALLAIQSLELDSKSCFKGTHPDASNRIFDNLSQHSEIGDEISQAMS
ECCCCCCEEEHHHHHHHHHHHCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
IVTLQSMTKIDIAPMSDLNFSDMLGEALYQINVNKR
HHHHHHHHEEECCCCCCCCHHHHHHHHHEEEECCCC
>Mature Secondary Structure 
SAVASRVAPENESKLVELSNKHDFSLLFLKESGFTFRVNTKTKVIKIPYGGLEYLWTFS
CCHHHHCCCCCCCCEEEECCCCCEEEEEEECCCCEEEEECCEEEEEECCCCHHHHHHHH
LKAWLLYQAYAQAQLNGEQNLDLETINGYAGTSQLTEYLKESLYAESYDPNKNFSALISV
HHHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEH
EEIDQEVATEIFLCALGWIIWHEIAHIELGHSSLEINTLSIQEEKDADLYSTNWILSSST
HHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEEEEEEECCCCCCCEEECCEEEECCE
IAAESKKRIVGITIALLAIQSLELDSKSCFKGTHPDASNRIFDNLSQHSEIGDEISQAMS
ECCCCCCEEEHHHHHHHHHHHCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
IVTLQSMTKIDIAPMSDLNFSDMLGEALYQINVNKR
HHHHHHHHEEECCCCCCCCHHHHHHHHHEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2452152; 8905232; 9278503 [H]