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The map label for this gene is gfa [H]

Identifier: 157963202

GI number: 157963202

Start: 4140714

End: 4141322

Strand: Reverse

Name: gfa [H]

Synonym: Spea_3387

Alternate gene names: 157963202

Gene position: 4141322-4140714 (Counterclockwise)

Preceding gene: 157963203

Following gene: 157963201

Centisome position: 80.03

GC content: 40.23

Gene sequence:

>609_bases
ATGTTGATTATGATTTCTAAAATATTAAGTTTTTTCCAGACAAAAAAGACGACTAAGGTGTTAATTCATCCATCTGTTGA
TAACAATAGATTCCCTGAATTACCTGGGTTTACAGGTGGTTCGTTAACTTGTCACTGTAAGAAAAAGCCAGTGATTGTAA
AGTTAAGCAGCCAAACTGCACATAACCATGTTTGTGGTTGCTCAAAGTGTTGGAAACCTAAAGGTGCCGTATTCTCTCAA
GTTGCAGTTATTTCTCGTGAGAATGTGAAAGTTACTGCAAATGAAAATAAGCTCGTCGTGGTTGATGCTCAGGCAGCAAT
TAATCGTTATGCCTGTAAAGATTGTGGTGTACACATGTATGGCCGTATCCATAATGAAGCTCATCCATTCTTTGGTTTAG
ACTTTGTTCACACAGAGCTAAGTAAGCAATCAGGTTGGGCGCCAGCAGAGTTTGCCGCTTTTGTATCTTCAATTATTGAA
ACGGGAACGAATCCTCAAGACATGCCTGCCATACGTGAGCGCTTACTGGAATTAGGCCTAGAGCCGTACGATTGTTTATC
ACCTGACCTAATGGATGCTATTGCTGCACATGTTGCTAAAAAGGCGTAA

Upstream 100 bases:

>100_bases
CCTTGCCCATGAGTGACATACTTATGCTGCATCATAATTGAGTTTCTAAATGTTAATTTAGAAGCCAATAGTTCAACGAT
TTAAGTTTTTCACTATATGT

Downstream 100 bases:

>100_bases
TTAGCCATCAGAATTATTAGCCAATTCTAAAATTTAAAGCCCTGTTTATGATAGACATCATGAGCGGGGCTTTTGTTTTC
AGCTCTCCTCCCCTTCTTTA

Product: glutathione-dependent formaldehyde-activating protein

Products: NA

Alternate protein names: S-(hydroxymethyl)glutathione synthase [H]

Number of amino acids: Translated: 202; Mature: 202

Protein sequence:

>202_residues
MLIMISKILSFFQTKKTTKVLIHPSVDNNRFPELPGFTGGSLTCHCKKKPVIVKLSSQTAHNHVCGCSKCWKPKGAVFSQ
VAVISRENVKVTANENKLVVVDAQAAINRYACKDCGVHMYGRIHNEAHPFFGLDFVHTELSKQSGWAPAEFAAFVSSIIE
TGTNPQDMPAIRERLLELGLEPYDCLSPDLMDAIAAHVAKKA

Sequences:

>Translated_202_residues
MLIMISKILSFFQTKKTTKVLIHPSVDNNRFPELPGFTGGSLTCHCKKKPVIVKLSSQTAHNHVCGCSKCWKPKGAVFSQ
VAVISRENVKVTANENKLVVVDAQAAINRYACKDCGVHMYGRIHNEAHPFFGLDFVHTELSKQSGWAPAEFAAFVSSIIE
TGTNPQDMPAIRERLLELGLEPYDCLSPDLMDAIAAHVAKKA
>Mature_202_residues
MLIMISKILSFFQTKKTTKVLIHPSVDNNRFPELPGFTGGSLTCHCKKKPVIVKLSSQTAHNHVCGCSKCWKPKGAVFSQ
VAVISRENVKVTANENKLVVVDAQAAINRYACKDCGVHMYGRIHNEAHPFFGLDFVHTELSKQSGWAPAEFAAFVSSIIE
TGTNPQDMPAIRERLLELGLEPYDCLSPDLMDAIAAHVAKKA

Specific function: Catalyzes the condensation of formaldehyde and glutathione to S-hydroxymethylglutathione [H]

COG id: COG3791

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gfa family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014185
- InterPro:   IPR006913
- InterPro:   IPR011057 [H]

Pfam domain/function: PF04828 GFA [H]

EC number: =4.4.1.22 [H]

Molecular weight: Translated: 22208; Mature: 22208

Theoretical pI: Translated: 8.59; Mature: 8.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
4.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIMISKILSFFQTKKTTKVLIHPSVDNNRFPELPGFTGGSLTCHCKKKPVIVKLSSQTA
CEEEHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCEEEEECCCCEEEEECCCCC
HNHVCGCSKCWKPKGAVFSQVAVISRENVKVTANENKLVVVDAQAAINRYACKDCGVHMY
CCCCCCCHHHCCCCCHHHHHHHHEECCCEEEEECCCEEEEEECHHHHHHHHHHHCCEEEE
GRIHNEAHPFFGLDFVHTELSKQSGWAPAEFAAFVSSIIETGTNPQDMPAIRERLLELGL
EEECCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCC
EPYDCLSPDLMDAIAAHVAKKA
CCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLIMISKILSFFQTKKTTKVLIHPSVDNNRFPELPGFTGGSLTCHCKKKPVIVKLSSQTA
CEEEHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCEEEEECCCCEEEEECCCCC
HNHVCGCSKCWKPKGAVFSQVAVISRENVKVTANENKLVVVDAQAAINRYACKDCGVHMY
CCCCCCCHHHCCCCCHHHHHHHHEECCCEEEEECCCEEEEEECHHHHHHHHHHHCCEEEE
GRIHNEAHPFFGLDFVHTELSKQSGWAPAEFAAFVSSIIETGTNPQDMPAIRERLLELGL
EEECCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCC
EPYDCLSPDLMDAIAAHVAKKA
CCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481431 [H]