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The map label for this gene is hyfR [H]

Identifier: 157963182

GI number: 157963182

Start: 4112640

End: 4114166

Strand: Reverse

Name: hyfR [H]

Synonym: Spea_3367

Alternate gene names: 157963182

Gene position: 4114166-4112640 (Counterclockwise)

Preceding gene: 157963186

Following gene: 157963180

Centisome position: 79.51

GC content: 45.78

Gene sequence:

>1527_bases
ATGAAGGAACAATTTTATTATCAGGCGACTCACTGTCTATGCAGCAGTTTGTCACTCGAAGTCGCTCTACAAAGTTACTA
CGAATTTGTTAAGCAGTACCTGCCTATCGATGGTATTTTTCTCAATATCTATCGCCCCGAACACGGTGATATTCAGTTCT
TGTCTCATGTTAATGAACAACAGGCGGTAGCACTGGATAGACAGGTGAAAATTTCGCCTGAAATGGAAGCTGTGTTGCAG
GAAACTGACCGACCAGTGATCCGTATTATTAATGATATTAGCCTGGATAAGGTGACGGGGTTTGTTGCGCCGCAATTGAT
CCCTGAAATCGCTTCGGTTGTTTTGTTGCGCATGGTTAGCAGCGATACCCATCTCGGTGTGGTCGGATTTTATTCCAAAA
AGAAAGCACAGTTTAAACCTTTGCATGCGGACCTAATTGAACCCCACAAAGACACATTATCTCTGATCACCGCCTTCAAT
TTACGTGGTCGTAACTTGCTTAGAGCTAACCAAGCATTGAGCCTAGAAAATGATAGTCTTAAGCGAGCCATGTTTTGTCC
TGACGGTGTTGTGGGAGCCGAAGGGGGTTTGAAGAAGGTAATGGTGCAAGTCGATGCGATTGCTGCTTTGAATACCAGTG
TATTAATGCAGGGTGAAACTGGCTGCGGCAAAGAAGTCATTGCGAATGCGATTCACGCAAAATCAAATCGTGCAGGGAAG
CCTTTTATTAAAGTGAACTGCGGCGCCATTCCTGAAACACTCATAGACTCGGAGCTATTTGGTTATGAGAAAGGAGCGTT
TACTGGAGCCGAGACCCGTAAAGCTGGTTACTTTGAGCAGGCGAATGGCGGCACCATCTTCTTAGATGAGATTGGCGAGC
TGCCACTCTCTGCGCAGGTGCGCTTATTACGTGTTCTGCAAAACAGTTCAATTACCCGAGTGGGTGGCTATGAAAATGTT
GACCTGGATATTCGGGTTATTGCTGCGACCCATAGAAACCTACAAGCCATGGTACATGAGAAAACCTTTAGAGAAGATCT
CTGGTATCGATTAGCTGTTTTTCCTATTGATATTCCGGCGCTACGCCAGCGTCGCAGTGACATTCCACTGTTAGTGCAAC
ACTTTATCGAAATGTTGGCCGCAAAGTTTAACTTGGAAAAGTTACCTAGAGTGATGCCAGAGCAATTGAGTATTTTGAAT
AATTACAGCTGGCCGGGTAACGTGCGCGAGCTGATCAATGTACTTGAGCGAGCCATCATTCAAAAGCCCAGAGGACCGTT
AACCTTTGATTTGTTGCAGGCGCAATCAGATGAGGAAGTGACCACGAAAGCGGGACAAACCATTATCGTTGATCCCAGCC
ACGCCAGTGACAAATTAGTGCCACTCGAGACCATGATAAGTAAATACATTAGCCATGCAATGCGTATAACCGGCGGAAAA
CTCTATGGACCGGGCGGCGCAGCAGAGCTATTGGACATTAACCCTAACACTCTCAAGAGCAAGATGAAGAAGCTCGGATT
ATGCTAA

Upstream 100 bases:

>100_bases
TTAATTGTTGTCTATTTTTGGTTATTTATGAACAGGAACACAGAATCACGAAATACAATGATTTACCATCACGAAACTTC
GTGGCTAACCCGAGATATCA

Downstream 100 bases:

>100_bases
AGCAGCTTCCAGGGATGATGGGTTAACAGCAAAGTTAATTAGGCAATAAAAAAACACTTCCGAGGAAGTGTTTTTTTATG
ACTTTCTAAAGAGGTTCTCG

Product: response regulator receiver protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 508; Mature: 508

Protein sequence:

>508_residues
MKEQFYYQATHCLCSSLSLEVALQSYYEFVKQYLPIDGIFLNIYRPEHGDIQFLSHVNEQQAVALDRQVKISPEMEAVLQ
ETDRPVIRIINDISLDKVTGFVAPQLIPEIASVVLLRMVSSDTHLGVVGFYSKKKAQFKPLHADLIEPHKDTLSLITAFN
LRGRNLLRANQALSLENDSLKRAMFCPDGVVGAEGGLKKVMVQVDAIAALNTSVLMQGETGCGKEVIANAIHAKSNRAGK
PFIKVNCGAIPETLIDSELFGYEKGAFTGAETRKAGYFEQANGGTIFLDEIGELPLSAQVRLLRVLQNSSITRVGGYENV
DLDIRVIAATHRNLQAMVHEKTFREDLWYRLAVFPIDIPALRQRRSDIPLLVQHFIEMLAAKFNLEKLPRVMPEQLSILN
NYSWPGNVRELINVLERAIIQKPRGPLTFDLLQAQSDEEVTTKAGQTIIVDPSHASDKLVPLETMISKYISHAMRITGGK
LYGPGGAAELLDINPNTLKSKMKKLGLC

Sequences:

>Translated_508_residues
MKEQFYYQATHCLCSSLSLEVALQSYYEFVKQYLPIDGIFLNIYRPEHGDIQFLSHVNEQQAVALDRQVKISPEMEAVLQ
ETDRPVIRIINDISLDKVTGFVAPQLIPEIASVVLLRMVSSDTHLGVVGFYSKKKAQFKPLHADLIEPHKDTLSLITAFN
LRGRNLLRANQALSLENDSLKRAMFCPDGVVGAEGGLKKVMVQVDAIAALNTSVLMQGETGCGKEVIANAIHAKSNRAGK
PFIKVNCGAIPETLIDSELFGYEKGAFTGAETRKAGYFEQANGGTIFLDEIGELPLSAQVRLLRVLQNSSITRVGGYENV
DLDIRVIAATHRNLQAMVHEKTFREDLWYRLAVFPIDIPALRQRRSDIPLLVQHFIEMLAAKFNLEKLPRVMPEQLSILN
NYSWPGNVRELINVLERAIIQKPRGPLTFDLLQAQSDEEVTTKAGQTIIVDPSHASDKLVPLETMISKYISHAMRITGGK
LYGPGGAAELLDINPNTLKSKMKKLGLC
>Mature_508_residues
MKEQFYYQATHCLCSSLSLEVALQSYYEFVKQYLPIDGIFLNIYRPEHGDIQFLSHVNEQQAVALDRQVKISPEMEAVLQ
ETDRPVIRIINDISLDKVTGFVAPQLIPEIASVVLLRMVSSDTHLGVVGFYSKKKAQFKPLHADLIEPHKDTLSLITAFN
LRGRNLLRANQALSLENDSLKRAMFCPDGVVGAEGGLKKVMVQVDAIAALNTSVLMQGETGCGKEVIANAIHAKSNRAGK
PFIKVNCGAIPETLIDSELFGYEKGAFTGAETRKAGYFEQANGGTIFLDEIGELPLSAQVRLLRVLQNSSITRVGGYENV
DLDIRVIAATHRNLQAMVHEKTFREDLWYRLAVFPIDIPALRQRRSDIPLLVQHFIEMLAAKFNLEKLPRVMPEQLSILN
NYSWPGNVRELINVLERAIIQKPRGPLTFDLLQAQSDEEVTTKAGQTIIVDPSHASDKLVPLETMISKYISHAMRITGGK
LYGPGGAAELLDINPNTLKSKMKKLGLC

Specific function: Required for induction of expression of the hydrogenase- 4 structural genes [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI87082117, Length=353, Percent_Identity=41.3597733711048, Blast_Score=265, Evalue=7e-72,
Organism=Escherichia coli, GI1789087, Length=519, Percent_Identity=33.718689788054, Blast_Score=259, Evalue=3e-70,
Organism=Escherichia coli, GI1788550, Length=223, Percent_Identity=55.1569506726457, Blast_Score=257, Evalue=1e-69,
Organism=Escherichia coli, GI1790437, Length=316, Percent_Identity=41.7721518987342, Blast_Score=246, Evalue=3e-66,
Organism=Escherichia coli, GI1790299, Length=338, Percent_Identity=41.1242603550296, Blast_Score=237, Evalue=1e-63,
Organism=Escherichia coli, GI1788905, Length=287, Percent_Identity=40.0696864111498, Blast_Score=219, Evalue=2e-58,
Organism=Escherichia coli, GI1789233, Length=392, Percent_Identity=37.7551020408163, Blast_Score=216, Evalue=3e-57,
Organism=Escherichia coli, GI87081872, Length=232, Percent_Identity=44.8275862068966, Blast_Score=211, Evalue=6e-56,
Organism=Escherichia coli, GI87082152, Length=231, Percent_Identity=45.021645021645, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI1786524, Length=249, Percent_Identity=43.7751004016064, Blast_Score=209, Evalue=3e-55,
Organism=Escherichia coli, GI1787583, Length=232, Percent_Identity=41.3793103448276, Blast_Score=189, Evalue=4e-49,
Organism=Escherichia coli, GI87081858, Length=295, Percent_Identity=32.5423728813559, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1789828, Length=260, Percent_Identity=32.3076923076923, Blast_Score=132, Evalue=5e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 56489; Mature: 56489

Theoretical pI: Translated: 7.36; Mature: 7.36

Prosite motif: PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEQFYYQATHCLCSSLSLEVALQSYYEFVKQYLPIDGIFLNIYRPEHGDIQFLSHVNEQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHCCHH
QAVALDRQVKISPEMEAVLQETDRPVIRIINDISLDKVTGFVAPQLIPEIASVVLLRMVS
HHHHHCCEEECCHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHC
SDTHLGVVGFYSKKKAQFKPLHADLIEPHKDTLSLITAFNLRGRNLLRANQALSLENDSL
CCCCEEEEEECCCCCCCCCCCHHHHCCCCHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCH
KRAMFCPDGVVGAEGGLKKVMVQVDAIAALNTSVLMQGETGCGKEVIANAIHAKSNRAGK
HHHEECCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHCCCCCCC
PFIKVNCGAIPETLIDSELFGYEKGAFTGAETRKAGYFEQANGGTIFLDEIGELPLSAQV
CEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCEEEEECCCCCCHHHHH
RLLRVLQNSSITRVGGYENVDLDIRVIAATHRNLQAMVHEKTFREDLWYRLAVFPIDIPA
HHHHHHHCCCEEECCCCCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHEEEEEEECCCHH
LRQRRSDIPLLVQHFIEMLAAKFNLEKLPRVMPEQLSILNNYSWPGNVRELINVLERAII
HHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
QKPRGPLTFDLLQAQSDEEVTTKAGQTIIVDPSHASDKLVPLETMISKYISHAMRITGGK
HCCCCCCCHHHHHCCCCCHHHHHCCCEEEECCCCCCCCEECHHHHHHHHHHHHHHHCCCE
LYGPGGAAELLDINPNTLKSKMKKLGLC
EECCCCCCEEEECCHHHHHHHHHHHCCC
>Mature Secondary Structure
MKEQFYYQATHCLCSSLSLEVALQSYYEFVKQYLPIDGIFLNIYRPEHGDIQFLSHVNEQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHCCHH
QAVALDRQVKISPEMEAVLQETDRPVIRIINDISLDKVTGFVAPQLIPEIASVVLLRMVS
HHHHHCCEEECCHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHC
SDTHLGVVGFYSKKKAQFKPLHADLIEPHKDTLSLITAFNLRGRNLLRANQALSLENDSL
CCCCEEEEEECCCCCCCCCCCHHHHCCCCHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCH
KRAMFCPDGVVGAEGGLKKVMVQVDAIAALNTSVLMQGETGCGKEVIANAIHAKSNRAGK
HHHEECCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHCCCCCCC
PFIKVNCGAIPETLIDSELFGYEKGAFTGAETRKAGYFEQANGGTIFLDEIGELPLSAQV
CEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCEEEEECCCCCCHHHHH
RLLRVLQNSSITRVGGYENVDLDIRVIAATHRNLQAMVHEKTFREDLWYRLAVFPIDIPA
HHHHHHHCCCEEECCCCCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHEEEEEEECCCHH
LRQRRSDIPLLVQHFIEMLAAKFNLEKLPRVMPEQLSILNNYSWPGNVRELINVLERAII
HHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
QKPRGPLTFDLLQAQSDEEVTTKAGQTIIVDPSHASDKLVPLETMISKYISHAMRITGGK
HCCCCCCCHHHHHCCCCCHHHHHCCCEEEECCCCCCCCEECHHHHHHHHHHHHHHHCCCE
LYGPGGAAELLDINPNTLKSKMKKLGLC
EECCCCCCEEEECCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]