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The map label for this gene is ubiH [H]

Identifier: 157963121

GI number: 157963121

Start: 4048910

End: 4050190

Strand: Reverse

Name: ubiH [H]

Synonym: Spea_3305

Alternate gene names: 157963121

Gene position: 4050190-4048910 (Counterclockwise)

Preceding gene: 157963122

Following gene: 157963120

Centisome position: 78.27

GC content: 51.29

Gene sequence:

>1281_bases
ATGGCTAATCCTAACGAGCTGGCTGATCTAGCAAGTAGCACACAGATAAATGAAGTCGATATCGCGATTGTTGGCGGTGC
GATGGCGGGGGCAACTTTAGCCCTAGGCCTAGCAGAACTTGCTAAACAGCAAGCGAGCATTGGTGCCGATGCGCCAATTA
AAATAGCCCTTATCGAGGCGCATAAGCCAAGCAATGAACACCCAGGGTTCGACGCCCGCTCTATCGCTATCTCCCATGGC
TCTATCTTTGAGCTAAAACAGCTCGGCCTTTGGCAGCATCTTGACCCCTTAGCGACGCCAATTAGCAATATTCATGTATC
CGATCGCGGCCACTTTGGAATGACGGAGCTTAATAGCGAGTCACTTCACCTGCCATATCTTGGCCAAGTGGTCGAGCTCG
AAGCTGTGGGCAACAAGCTGTTTAGCCTGTTAGAGAAAAGCGATATTGCACTTTACTGTCCGAGTCGCTTGCACAGCGTT
GAAGCGCGAATCGACTCACAACTGCTAACTCTCGAAGATGGCCAGCAGATCTCGGCAAAACTCGTGGTTGCCGCCGACGG
GGTAAACTCTAAGGTGCGCCAAGCCTTTAAGCAGCCATTAGAACAAGTTGAGTTTGGTCAGAGCGCAATAATTGCCAATA
TTCATACCGAGCAGCCACATGCTGGCTGGGCCTATGAGCGCTTTACCCAAACCGGACCATTAGCCTTATTGCCTATGGCC
GATAACAAGGGGGACTCGCGCTTATCTTTAGTGTGGGCATTACCGCCAGAAGAAGCTGAGCGGCTATTAACGGCGCCAAA
GAGTGAGTTTATCGACAAGCTACAGCAAGCCTTTGGCTACCGAGCCGGGCGATTTGTCGATGTCGGTAATCGCTTTAGCT
ACCCGTTGACCCTGTCGTATATGCCAAGGCCTATCTACCACCGCACCGTCTTTTTAGGCAATGCCGCCCAGACCTTGCAC
CCAATTGCAGGCCAAGGTTTTAACTTAGGTCTGCGGGATGTGGTATGCCTGATTGAGGTACTTAAGCAAGTGCTTGCTAA
TCCAGAAGGTCAGGCTGGCAAGGATGTTGGTAGTAGCGAATTGACCCATCTCTTTCTTGAACGTCGTAAGACAGACCGAC
AAGGTACCATGAACAGTATCGAGTTTTTAGTGCGCGGTTTTTCTAATGATTATTGGCCGCTGGTGGTTGGGCGTAATCTT
GGGCTACGATTGCTGTCTTGGTTCCCGCCGTTAAAGGCACCTGTTGCACGCCGCGCAATGGGCTGGAACCAAGGCGAATA
G

Upstream 100 bases:

>100_bases
CTAAAACTCATACCTAAATACCGCCATCGCAGCAATTTGCAATGGCGGTTTTTGTATCTGCAACACTACCAAATATTTTT
GTTATTGGGTCTATAAACTC

Downstream 100 bases:

>100_bases
GCTAAGTACAACCGTCTCCATTAGCTAAGCATCATTATGGCAAGCTAGTGGCCGCGTAATTCACGCTATGAGCAGTATTT
TTAGTAAGGGTAAAAGATGT

Product: 2-polyprenyl-6-methoxyphenol 4-hydroxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MANPNELADLASSTQINEVDIAIVGGAMAGATLALGLAELAKQQASIGADAPIKIALIEAHKPSNEHPGFDARSIAISHG
SIFELKQLGLWQHLDPLATPISNIHVSDRGHFGMTELNSESLHLPYLGQVVELEAVGNKLFSLLEKSDIALYCPSRLHSV
EARIDSQLLTLEDGQQISAKLVVAADGVNSKVRQAFKQPLEQVEFGQSAIIANIHTEQPHAGWAYERFTQTGPLALLPMA
DNKGDSRLSLVWALPPEEAERLLTAPKSEFIDKLQQAFGYRAGRFVDVGNRFSYPLTLSYMPRPIYHRTVFLGNAAQTLH
PIAGQGFNLGLRDVVCLIEVLKQVLANPEGQAGKDVGSSELTHLFLERRKTDRQGTMNSIEFLVRGFSNDYWPLVVGRNL
GLRLLSWFPPLKAPVARRAMGWNQGE

Sequences:

>Translated_426_residues
MANPNELADLASSTQINEVDIAIVGGAMAGATLALGLAELAKQQASIGADAPIKIALIEAHKPSNEHPGFDARSIAISHG
SIFELKQLGLWQHLDPLATPISNIHVSDRGHFGMTELNSESLHLPYLGQVVELEAVGNKLFSLLEKSDIALYCPSRLHSV
EARIDSQLLTLEDGQQISAKLVVAADGVNSKVRQAFKQPLEQVEFGQSAIIANIHTEQPHAGWAYERFTQTGPLALLPMA
DNKGDSRLSLVWALPPEEAERLLTAPKSEFIDKLQQAFGYRAGRFVDVGNRFSYPLTLSYMPRPIYHRTVFLGNAAQTLH
PIAGQGFNLGLRDVVCLIEVLKQVLANPEGQAGKDVGSSELTHLFLERRKTDRQGTMNSIEFLVRGFSNDYWPLVVGRNL
GLRLLSWFPPLKAPVARRAMGWNQGE
>Mature_425_residues
ANPNELADLASSTQINEVDIAIVGGAMAGATLALGLAELAKQQASIGADAPIKIALIEAHKPSNEHPGFDARSIAISHGS
IFELKQLGLWQHLDPLATPISNIHVSDRGHFGMTELNSESLHLPYLGQVVELEAVGNKLFSLLEKSDIALYCPSRLHSVE
ARIDSQLLTLEDGQQISAKLVVAADGVNSKVRQAFKQPLEQVEFGQSAIIANIHTEQPHAGWAYERFTQTGPLALLPMAD
NKGDSRLSLVWALPPEEAERLLTAPKSEFIDKLQQAFGYRAGRFVDVGNRFSYPLTLSYMPRPIYHRTVFLGNAAQTLHP
IAGQGFNLGLRDVVCLIEVLKQVLANPEGQAGKDVGSSELTHLFLERRKTDRQGTMNSIEFLVRGFSNDYWPLVVGRNLG
LRLLSWFPPLKAPVARRAMGWNQGE

Specific function: Oxygenase that introduces the hydroxyl group at carbon four of 2-octaprenyl-6-methoxyphenol resulting in the formation of 2-octaprenyl-6-methoxy-1,4-benzoquinone [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiH/COQ6 family [H]

Homologues:

Organism=Homo sapiens, GI32967305, Length=398, Percent_Identity=28.643216080402, Blast_Score=125, Evalue=7e-29,
Organism=Homo sapiens, GI32967307, Length=319, Percent_Identity=30.0940438871473, Blast_Score=120, Evalue=3e-27,
Organism=Escherichia coli, GI1789274, Length=407, Percent_Identity=45.2088452088452, Blast_Score=343, Evalue=1e-95,
Organism=Escherichia coli, GI1789273, Length=420, Percent_Identity=32.8571428571429, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI1786883, Length=390, Percent_Identity=31.5384615384615, Blast_Score=154, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI25146631, Length=440, Percent_Identity=27.9545454545455, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI19920760, Length=457, Percent_Identity=27.7899343544858, Blast_Score=128, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042
- InterPro:   IPR018168
- InterPro:   IPR010971
- InterPro:   IPR011295 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.-

Molecular weight: Translated: 46641; Mature: 46510

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS01304 UBIH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANPNELADLASSTQINEVDIAIVGGAMAGATLALGLAELAKQQASIGADAPIKIALIEA
CCCHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEC
HKPSNEHPGFDARSIAISHGSIFELKQLGLWQHLDPLATPISNIHVSDRGHFGMTELNSE
CCCCCCCCCCCCEEEEECCCCHHHHHHHCHHHHCCHHHCCHHCEEECCCCCCCCCCCCCC
SLHLPYLGQVVELEAVGNKLFSLLEKSDIALYCPSRLHSVEARIDSQLLTLEDGQQISAK
CEECCCCCCEEHHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHCCEEEEECCCCCEEEE
LVVAADGVNSKVRQAFKQPLEQVEFGQSAIIANIHTEQPHAGWAYERFTQTGPLALLPMA
EEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHCCCCCEEEEEEC
DNKGDSRLSLVWALPPEEAERLLTAPKSEFIDKLQQAFGYRAGRFVDVGNRFSYPLTLSY
CCCCCCEEEEEEECCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEECCCCCCCCEEEEC
MPRPIYHRTVFLGNAAQTLHPIAGQGFNLGLRDVVCLIEVLKQVLANPEGQAGKDVGSSE
CCCCHHEEEEEECCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
LTHLFLERRKTDRQGTMNSIEFLVRGFSNDYWPLVVGRNLGLRLLSWFPPLKAPVARRAM
HHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHCCCCCHHHHHHHC
GWNQGE
CCCCCC
>Mature Secondary Structure 
ANPNELADLASSTQINEVDIAIVGGAMAGATLALGLAELAKQQASIGADAPIKIALIEA
CCHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEC
HKPSNEHPGFDARSIAISHGSIFELKQLGLWQHLDPLATPISNIHVSDRGHFGMTELNSE
CCCCCCCCCCCCEEEEECCCCHHHHHHHCHHHHCCHHHCCHHCEEECCCCCCCCCCCCCC
SLHLPYLGQVVELEAVGNKLFSLLEKSDIALYCPSRLHSVEARIDSQLLTLEDGQQISAK
CEECCCCCCEEHHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHCCEEEEECCCCCEEEE
LVVAADGVNSKVRQAFKQPLEQVEFGQSAIIANIHTEQPHAGWAYERFTQTGPLALLPMA
EEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHCCCCCEEEEEEC
DNKGDSRLSLVWALPPEEAERLLTAPKSEFIDKLQQAFGYRAGRFVDVGNRFSYPLTLSY
CCCCCCEEEEEEECCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEECCCCCCCCEEEEC
MPRPIYHRTVFLGNAAQTLHPIAGQGFNLGLRDVVCLIEVLKQVLANPEGQAGKDVGSSE
CCCCHHEEEEEECCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
LTHLFLERRKTDRQGTMNSIEFLVRGFSNDYWPLVVGRNLGLRLLSWFPPLKAPVARRAM
HHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHCCCCCHHHHHHHC
GWNQGE
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1339425; 9278503 [H]