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The map label for this gene is rhmD [H]

Identifier: 157963063

GI number: 157963063

Start: 3981385

End: 3982539

Strand: Reverse

Name: rhmD [H]

Synonym: Spea_3246

Alternate gene names: 157963063

Gene position: 3982539-3981385 (Counterclockwise)

Preceding gene: 157963064

Following gene: 157963061

Centisome position: 76.96

GC content: 47.36

Gene sequence:

>1155_bases
ATGGGCGCAAATATTGTTTCGGTTGAATTTATTCCGGTAAACGTGGCGGCGACTAACTGGAGCGAAAACACCGTTATCGT
TAAGGTTACCGATGAAAATGGTGTTTATGGACTGGGTGAGGCCGATGGTCCGCCAGAGTGCATGAAAGCCTTCTCGGAAA
TTGAGAATGAGCATAAGTGGCTCAACAACATTAAAGAAGCTGTGATTGGTAGAGATCCGTTAGAGTTCCGCGCAAACTAC
AACCGCATGTACGACACCACCAAGTGGATCGGTATGCGCGGCTTAGGTTTGTTTGCCATCTCTGGTATCGATATGGCGCT
ATATGATCTTGCCGGTAAGCAGTTAGGTGTACCAGCCTATAAGCTAATGGGCGGCGCCCAGAAAGCGCAGTTAACGCCAT
ATTTTACTCTGTATCCGTCGGTTGCCGCCGATGCGACACTAAGTGAGATTGTTGAAGCGTATAAACCGCTTATCGCTAAA
GCCAAAGAGCGTGGCGCTAAAGCCGTTAAAGTGTGCATCATTCCTAATGATAAGGTTAGTGACAAAGAGATTGTCGCTTA
CCTACGTGAGTTACGTGAGGTTATCGGTTGGGATATGGACATGATGGTTGATTGTCTATATCGCTGGACCGACTGGCAGA
AAGCTCGCTGGACATTCCGCCAGCTAGAAGATATCGATTTGTACTTTATCGAAGCCTGTTTGCAGCATGACGACTTGATT
GGTCATCAAAAGCTGGCTGCGGCGATTAATACCCGTTTATGTGGCGCCGAGATGTCGACCACACGTTTTGAAGCGCAAGA
GTGGCTCGAAAAAACCGGTATTTCGGTCGTGCAGTCTGACTACAACCGTTGTGGTGGTGTCACTGAGCTTTTACGTATTA
TGGACATCTGTGAGCACCATAATGCTCAGCTAATGCCTCATAACTGGAAAACAGGTATTACTGCCGCGGCGGCGCGTCAC
TTTGGTATCGTTTGCCATATCTCTGAGTATGTAGAGTATCTACACCCAGATTTCTGGAACGGCACCTTGACCCAGCAGCT
AACACTGAATGAGCCAAAGATTATCGATGGCGCCATTGAAGTCAGTGATAAGCCGGGATTAGGTATCGAGCTAAATATTG
AATTTGTTGAGCAGGTAACGGGTCACAAGTTCTAA

Upstream 100 bases:

>100_bases
CCAGAACTGTTCAAATCAACAGTTACACAGGCTGCAAAAAACAACGCAGAACAGGCTGAGCCTGCTCAAGCTGAATTAGC
AAAAGTTGAGGGATAACGAG

Downstream 100 bases:

>100_bases
CACGTTGTCTCATAAGCTAGTGTTAGCTCTTAGCTGTAGCTCTTTATATTAGCTTCGAGCTAATTCAGCGGACAGATTTA
AAAAGTGCCGATAAGCATGG

Product: mandelate racemase/muconate lactonizing protein

Products: NA

Alternate protein names: RhamD [H]

Number of amino acids: Translated: 384; Mature: 383

Protein sequence:

>384_residues
MGANIVSVEFIPVNVAATNWSENTVIVKVTDENGVYGLGEADGPPECMKAFSEIENEHKWLNNIKEAVIGRDPLEFRANY
NRMYDTTKWIGMRGLGLFAISGIDMALYDLAGKQLGVPAYKLMGGAQKAQLTPYFTLYPSVAADATLSEIVEAYKPLIAK
AKERGAKAVKVCIIPNDKVSDKEIVAYLRELREVIGWDMDMMVDCLYRWTDWQKARWTFRQLEDIDLYFIEACLQHDDLI
GHQKLAAAINTRLCGAEMSTTRFEAQEWLEKTGISVVQSDYNRCGGVTELLRIMDICEHHNAQLMPHNWKTGITAAAARH
FGIVCHISEYVEYLHPDFWNGTLTQQLTLNEPKIIDGAIEVSDKPGLGIELNIEFVEQVTGHKF

Sequences:

>Translated_384_residues
MGANIVSVEFIPVNVAATNWSENTVIVKVTDENGVYGLGEADGPPECMKAFSEIENEHKWLNNIKEAVIGRDPLEFRANY
NRMYDTTKWIGMRGLGLFAISGIDMALYDLAGKQLGVPAYKLMGGAQKAQLTPYFTLYPSVAADATLSEIVEAYKPLIAK
AKERGAKAVKVCIIPNDKVSDKEIVAYLRELREVIGWDMDMMVDCLYRWTDWQKARWTFRQLEDIDLYFIEACLQHDDLI
GHQKLAAAINTRLCGAEMSTTRFEAQEWLEKTGISVVQSDYNRCGGVTELLRIMDICEHHNAQLMPHNWKTGITAAAARH
FGIVCHISEYVEYLHPDFWNGTLTQQLTLNEPKIIDGAIEVSDKPGLGIELNIEFVEQVTGHKF
>Mature_383_residues
GANIVSVEFIPVNVAATNWSENTVIVKVTDENGVYGLGEADGPPECMKAFSEIENEHKWLNNIKEAVIGRDPLEFRANYN
RMYDTTKWIGMRGLGLFAISGIDMALYDLAGKQLGVPAYKLMGGAQKAQLTPYFTLYPSVAADATLSEIVEAYKPLIAKA
KERGAKAVKVCIIPNDKVSDKEIVAYLRELREVIGWDMDMMVDCLYRWTDWQKARWTFRQLEDIDLYFIEACLQHDDLIG
HQKLAAAINTRLCGAEMSTTRFEAQEWLEKTGISVVQSDYNRCGGVTELLRIMDICEHHNAQLMPHNWKTGITAAAARHF
GIVCHISEYVEYLHPDFWNGTLTQQLTLNEPKIIDGAIEVSDKPGLGIELNIEFVEQVTGHKF

Specific function: Catalyzes the dehydration of L-rhamnonate to 2-keto-3- deoxy-L-rhamnonate (KDR) [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. RhamD subfamily [H]

Homologues:

Organism=Escherichia coli, GI48994953, Length=366, Percent_Identity=26.5027322404372, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI226510960, Length=360, Percent_Identity=27.5, Blast_Score=95, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =4.2.1.90 [H]

Molecular weight: Translated: 43228; Mature: 43097

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGANIVSVEFIPVNVAATNWSENTVIVKVTDENGVYGLGEADGPPECMKAFSEIENEHKW
CCCCEEEEEEEEEEEEEECCCCCEEEEEEECCCCEEECCCCCCCHHHHHHHHHHHHHHHH
LNNIKEAVIGRDPLEFRANYNRMYDTTKWIGMRGLGLFAISGIDMALYDLAGKQLGVPAY
HHHHHHHHHCCCCHHHHCCCHHHHHHHHHHCCCCCCEEEECCCHHHHHHHCCCCCCCCHH
KLMGGAQKAQLTPYFTLYPSVAADATLSEIVEAYKPLIAKAKERGAKAVKVCIIPNDKVS
HHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCC
DKEIVAYLRELREVIGWDMDMMVDCLYRWTDWQKARWTFRQLEDIDLYFIEACLQHDDLI
HHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCC
GHQKLAAAINTRLCGAEMSTTRFEAQEWLEKTGISVVQSDYNRCGGVTELLRIMDICEHH
CHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHCCCHHHHHHHHHHHHCC
NAQLMPHNWKTGITAAAARHFGIVCHISEYVEYLHPDFWNGTLTQQLTLNEPKIIDGAIE
CCEECCCCCCCCHHHHHHHHHHHEEEHHHHHHHHCCCCCCCCEEEEEECCCCCEEECEEE
VSDKPGLGIELNIEFVEQVTGHKF
ECCCCCCEEEEEHHHHHHHCCCCC
>Mature Secondary Structure 
GANIVSVEFIPVNVAATNWSENTVIVKVTDENGVYGLGEADGPPECMKAFSEIENEHKW
CCCEEEEEEEEEEEEEECCCCCEEEEEEECCCCEEECCCCCCCHHHHHHHHHHHHHHHH
LNNIKEAVIGRDPLEFRANYNRMYDTTKWIGMRGLGLFAISGIDMALYDLAGKQLGVPAY
HHHHHHHHHCCCCHHHHCCCHHHHHHHHHHCCCCCCEEEECCCHHHHHHHCCCCCCCCHH
KLMGGAQKAQLTPYFTLYPSVAADATLSEIVEAYKPLIAKAKERGAKAVKVCIIPNDKVS
HHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCC
DKEIVAYLRELREVIGWDMDMMVDCLYRWTDWQKARWTFRQLEDIDLYFIEACLQHDDLI
HHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCC
GHQKLAAAINTRLCGAEMSTTRFEAQEWLEKTGISVVQSDYNRCGGVTELLRIMDICEHH
CHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHCCCHHHHHHHHHHHHCC
NAQLMPHNWKTGITAAAARHFGIVCHISEYVEYLHPDFWNGTLTQQLTLNEPKIIDGAIE
CCEECCCCCCCCHHHHHHHHHHHEEEHHHHHHHHCCCCCCCCEEEEEECCCCCEEECEEE
VSDKPGLGIELNIEFVEQVTGHKF
ECCCCCCEEEEEHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA