Click here to switch to the map view.

The map label for this gene is purP [H]

Identifier: 157963061

GI number: 157963061

Start: 3978674

End: 3980059

Strand: Reverse

Name: purP [H]

Synonym: Spea_3244

Alternate gene names: 157963061

Gene position: 3980059-3978674 (Counterclockwise)

Preceding gene: 157963063

Following gene: 157963059

Centisome position: 76.92

GC content: 49.49

Gene sequence:

>1386_bases
ATGAGTACGCCAAGCAATAAACAACATGCTTCAACTAATGAAGAGCCCGTAGGAGCTTGTGCTTCTGCCCAACCTGCAAG
TGGTGGGTTGTTAGAGCGCTTTTTTAAATTATCAGCCCATAACACTTCAGTCTCAACTGAGGTGATTGCGGGTATCACCA
CCTTTATGACCATGGTGTATATCGTGTTCGTGAATCCACAAATCCTATCTGCTGCTGGTATGGATGCCAGTGCGGTGTTT
GTGGTTACCTGTTTGATCAGCGCTATCGGCTGTATCGGCATGGGCTTAATTGCCAATTTGCCAATCGCACTCGCGCCAGC
AATGGGACTCAATGCTTTTTTTGCCTTCTCTGTTGTCGTAGGCATGGGCGTGAGTTGGCAAATCGGTATGGGCACCATTT
TTTGGGGCGCCGTTTGTTTTGCTATCGTGTCGCTATTAGGGATCCGCTCGTGGATCTTAACCAATATCCCAAAATGTCTA
CGCATAGGTATTCCAAGTGGTATTGGTTTGCTAATCGCCATGGTTGGCTTTAGTAACGCGGGCATTGTGGTCGCAAGCCC
TGCGACTATGATCACCGTCGGTGACTTAAGCTCGCTGCAATGTGTGTTAGGCGCGTTAGGCTTCTTTATCATCGTTATTC
TAGCCTCTCGTGGTATCCATTCTGCAGTATTGGTGTCGATTGCTATCGTGACGGCTATTGCGGCCTTAATGGGCGATATC
GAGTACACCGGCATTGTGTCTATGCCACCAAGCATCGCCAATACCTTTGGTCAGCTAGATCTTGCTGGATCTTTAGATGT
GGCGCTAATGGGGGTGATTTTCTCGTTTGCGCTTGTGAGCCTATTTGATAGCTCTGGCACAATGATTGGCGTAACAGAAA
AGTGTGGCTTTACCGACAAGCGCGGCCGCTTTCCACGCATGAAGCAAGCCTTGTTGACCGACTCGGCAACTTCAGTCGCT
GGCGCCTATATGGGGACCTCTACGGTAACCGCTTATATTGAAAGTTCGGCAGGTGTCGCTGCGGGTGGCCGCACAGGCTT
AACTGCGATCGTCGCCGGTCTACTGTTTATTCTGGTGATCTTCTTCTCGCCATTGGCGGCTATGGTACCGGGTTACGCGG
TTGCTGGCGCGCTAGTTTATGTGGGCATTTTGATGTCATCAGAGCTGGCTAAAATTGATGGTAAAGACTTAACCGAGTCG
GCTCCAGCCTTTATTACCGCGGTGATGATGCCGTTTACTTTCTCGATTACCGAAGGCGTGGCGGCAGGTTTTATTACCTA
CTGTGTGCTAAAAGCGGGTACCAATCGCTGGCGTGAAATCCACCCTGGCGTGGCGTTAGTGGCTCTGCTGTTTATTATCA
AATTTGCTTTTGTTGATGGGCATTAA

Upstream 100 bases:

>100_bases
CTTTCAGGGGATTAAGTGTGTGCGTCGTAAATTTAATATTGATGTTGCGCAGCTGTTTGGGACGCGGGTTGAGTGAAGTA
AAACTGGAAATAATCTATTT

Downstream 100 bases:

>100_bases
TTCTACTCGCTTAAATGAGTAGTCTGTAAAAGTGATAAACAAATAAAAGGCTCAGAAGGATGATCTTCTGAGCCTTTTTT
ATATGACTAGTCAACTAAGT

Product: xanthine/uracil/vitamin C permease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 460

Protein sequence:

>461_residues
MSTPSNKQHASTNEEPVGACASAQPASGGLLERFFKLSAHNTSVSTEVIAGITTFMTMVYIVFVNPQILSAAGMDASAVF
VVTCLISAIGCIGMGLIANLPIALAPAMGLNAFFAFSVVVGMGVSWQIGMGTIFWGAVCFAIVSLLGIRSWILTNIPKCL
RIGIPSGIGLLIAMVGFSNAGIVVASPATMITVGDLSSLQCVLGALGFFIIVILASRGIHSAVLVSIAIVTAIAALMGDI
EYTGIVSMPPSIANTFGQLDLAGSLDVALMGVIFSFALVSLFDSSGTMIGVTEKCGFTDKRGRFPRMKQALLTDSATSVA
GAYMGTSTVTAYIESSAGVAAGGRTGLTAIVAGLLFILVIFFSPLAAMVPGYAVAGALVYVGILMSSELAKIDGKDLTES
APAFITAVMMPFTFSITEGVAAGFITYCVLKAGTNRWREIHPGVALVALLFIIKFAFVDGH

Sequences:

>Translated_461_residues
MSTPSNKQHASTNEEPVGACASAQPASGGLLERFFKLSAHNTSVSTEVIAGITTFMTMVYIVFVNPQILSAAGMDASAVF
VVTCLISAIGCIGMGLIANLPIALAPAMGLNAFFAFSVVVGMGVSWQIGMGTIFWGAVCFAIVSLLGIRSWILTNIPKCL
RIGIPSGIGLLIAMVGFSNAGIVVASPATMITVGDLSSLQCVLGALGFFIIVILASRGIHSAVLVSIAIVTAIAALMGDI
EYTGIVSMPPSIANTFGQLDLAGSLDVALMGVIFSFALVSLFDSSGTMIGVTEKCGFTDKRGRFPRMKQALLTDSATSVA
GAYMGTSTVTAYIESSAGVAAGGRTGLTAIVAGLLFILVIFFSPLAAMVPGYAVAGALVYVGILMSSELAKIDGKDLTES
APAFITAVMMPFTFSITEGVAAGFITYCVLKAGTNRWREIHPGVALVALLFIIKFAFVDGH
>Mature_460_residues
STPSNKQHASTNEEPVGACASAQPASGGLLERFFKLSAHNTSVSTEVIAGITTFMTMVYIVFVNPQILSAAGMDASAVFV
VTCLISAIGCIGMGLIANLPIALAPAMGLNAFFAFSVVVGMGVSWQIGMGTIFWGAVCFAIVSLLGIRSWILTNIPKCLR
IGIPSGIGLLIAMVGFSNAGIVVASPATMITVGDLSSLQCVLGALGFFIIVILASRGIHSAVLVSIAIVTAIAALMGDIE
YTGIVSMPPSIANTFGQLDLAGSLDVALMGVIFSFALVSLFDSSGTMIGVTEKCGFTDKRGRFPRMKQALLTDSATSVAG
AYMGTSTVTAYIESSAGVAAGGRTGLTAIVAGLLFILVIFFSPLAAMVPGYAVAGALVYVGILMSSELAKIDGKDLTESA
PAFITAVMMPFTFSITEGVAAGFITYCVLKAGTNRWREIHPGVALVALLFIIKFAFVDGH

Specific function: High-affinity uptake of adenine [H]

COG id: COG2252

COG function: function code R; Permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the xanthine/uracil permease family. AzgA purine transporter (TC 2.A.1.40) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790150, Length=440, Percent_Identity=64.3181818181818, Blast_Score=570, Evalue=1e-164,
Organism=Escherichia coli, GI87082309, Length=431, Percent_Identity=60.3248259860789, Blast_Score=535, Evalue=1e-153,
Organism=Escherichia coli, GI1790499, Length=439, Percent_Identity=37.8132118451025, Blast_Score=283, Evalue=2e-77,
Organism=Escherichia coli, GI48994909, Length=431, Percent_Identity=36.1948955916473, Blast_Score=259, Evalue=3e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006043 [H]

Pfam domain/function: PF00860 Xan_ur_permease [H]

EC number: NA

Molecular weight: Translated: 47525; Mature: 47394

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTPSNKQHASTNEEPVGACASAQPASGGLLERFFKLSAHNTSVSTEVIAGITTFMTMVY
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
IVFVNPQILSAAGMDASAVFVVTCLISAIGCIGMGLIANLPIALAPAMGLNAFFAFSVVV
HHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCHHHHHHHHHHHHHH
GMGVSWQIGMGTIFWGAVCFAIVSLLGIRSWILTNIPKCLRIGIPSGIGLLIAMVGFSNA
HCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHCCCCC
GIVVASPATMITVGDLSSLQCVLGALGFFIIVILASRGIHSAVLVSIAIVTAIAALMGDI
CEEEECCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCC
EYTGIVSMPPSIANTFGQLDLAGSLDVALMGVIFSFALVSLFDSSGTMIGVTEKCGFTDK
CCCEEECCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCC
RGRFPRMKQALLTDSATSVAGAYMGTSTVTAYIESSAGVAAGGRTGLTAIVAGLLFILVI
CCCCHHHHHHHHHCCHHHHHHHHHCHHHHHHEEECCCCCCCCCCCCHHHHHHHHHHHHHH
FFSPLAAMVPGYAVAGALVYVGILMSSELAKIDGKDLTESAPAFITAVMMPFTFSITEGV
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
AAGFITYCVLKAGTNRWREIHPGVALVALLFIIKFAFVDGH
HHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
STPSNKQHASTNEEPVGACASAQPASGGLLERFFKLSAHNTSVSTEVIAGITTFMTMVY
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
IVFVNPQILSAAGMDASAVFVVTCLISAIGCIGMGLIANLPIALAPAMGLNAFFAFSVVV
HHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCHHHHHHHHHHHHHH
GMGVSWQIGMGTIFWGAVCFAIVSLLGIRSWILTNIPKCLRIGIPSGIGLLIAMVGFSNA
HCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHCCCCC
GIVVASPATMITVGDLSSLQCVLGALGFFIIVILASRGIHSAVLVSIAIVTAIAALMGDI
CEEEECCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCC
EYTGIVSMPPSIANTFGQLDLAGSLDVALMGVIFSFALVSLFDSSGTMIGVTEKCGFTDK
CCCEEECCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCC
RGRFPRMKQALLTDSATSVAGAYMGTSTVTAYIESSAGVAAGGRTGLTAIVAGLLFILVI
CCCCHHHHHHHHHCCHHHHHHHHHCHHHHHHEEECCCCCCCCCCCCHHHHHHHHHHHHHH
FFSPLAAMVPGYAVAGALVYVGILMSSELAKIDGKDLTESAPAFITAVMMPFTFSITEGV
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
AAGFITYCVLKAGTNRWREIHPGVALVALLFIIKFAFVDGH
HHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503 [H]