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The map label for this gene is phsA [H]

Identifier: 157963005

GI number: 157963005

Start: 3908137

End: 3910407

Strand: Reverse

Name: phsA [H]

Synonym: Spea_3188

Alternate gene names: 157963005

Gene position: 3910407-3908137 (Counterclockwise)

Preceding gene: 157963006

Following gene: 157963004

Centisome position: 75.57

GC content: 48.83

Gene sequence:

>2271_bases
ATGTTGTCTCGCCGCGATTTTTTGGCAAAAGGTAGCGCCTTTTGCGCTATAACAGTTTATGCCCCCGGAACGTTAGGGGC
GCAAAGTAAAATATTAACACCAGCAACGCCATTTATTAAAGAAATAAACAGTATCTGCGAAATGTGTTCAACTCGGTGTC
CCATTACCGTTGAAAGCAGACTGGGCAAGTTATTCATTCGGGGGAACCTGAACGCTAAGAGCTTTGGCGGTAAGGTATGC
GCAAGAGGTACATCAGGTAGCAAGCTGCTCAATGATCCTCATCGATTGGTAAAACCGATTAAGCGAACCGGACCTCGAGG
TTCGGGGCAGTGGCAGGAAATTGAGTGGGAAGAAGCATACCAACTTATTGCGGTTAACCTAAATCAAGTGCGTGACCAAT
ACGGACCAGAAGCAATCGCAGTCTCGAGTAAATCTGGCTCATTATCCAATCATGCCTTTCACTTTGGTAAAGCCATTGGC
ACCCCAAATATCTTTTCACACGGCTCAACATGTCCGATCTCCTATGCGGTTGCCGCCAAGGTGATGTTCGGCAATGAGCT
TAAGCTGGATTTAGAAAACAGCAAATACATACTTAACTTCGGTCATAACCTGTATGAAGGGGTCAATATGTCAATGACCC
GAGGCTTGTTGGCGGCACAGGAAAAAGGCACGGTCAAGGTCGTGGTGTTTGAGCCTAGATTATCGGTGATGGCTGACAAA
GCAGATGAATGGCATGCCATTAAACCCGGCACAGACATTGCCGTTGTACTCGCTCTGAGTCATGTATTAATAGAAGAGCA
GTTGTACGATGAATCTTTTGTTCAGCAATATGTTAGTGGCTTCGAGGATTTTGCGACTTCGATGGCGCGATACAGCCCTG
AGTGGGCAAGTGAGGTATGCGGGATTTCAGCTGGCGACATTCGTCGTATCGCCCGAGAACTCGCCGCAGCCGCCCCCGCA
GCGATAGCGGACCATGGCCATCGCTGCACCTTTACACCTGAAGAGTTCGATCTTCGGCGTGGTATCTATGCCATAAACGT
GCTGCTGGGTAATATTGAACGTAAAGGAGGTGTGTATTTCGCTAAGAATTCTAAGCTGTACAACAAACTTGCTGGCGAAG
CGGTAGCCCCATCACTCGAAAAAATAAAAGTAGAAGGTTTACCAACCCCAGTGTTACCACGTATCGATTTAGCCCAAGCC
CAGTTTAAGTATGCTGCCAAAGCGGGTGGGCTCTATCAATCTATTATCCCTGCTGCCTTGCAGCAACAGCCCTACGCGAT
AAAGGCATGGGTGATGTCACGTTCTAACCCTGTGCAAACCGTCACCGATCGAACCCAAGTTCAAAAAGCACTCGAGGCGA
TGGATTTTGTTGTCGCCTGTGACGTATATATTAGCGAGAGCGCGGCCTATGCCGACGTTGTTCTGCCTGAGTCCACTTAT
CTTGAGCGTGACGAAGACATCAGTGACTATTCTGCTAAGAGCCCAGCCTACGCCTTGCGTCAGCAAGCAACCGATACCAT
AGGTAACACTCGACCAAGTTGGCAAATATTTAAAGAGATCGCTGACGCAATGGCGCTGGGACAATTTTTTCCTTGGCAAG
ATATTGCTGACTATCAACAGCGTCAGGTTGCAAACAACCCGGAATTATTACCGCAACTTAGAAAAGAAGGTTACCTCTCA
TTTGGCATCCCACTGTTGATGCGAGAACCAGATATGGTGGCGAAGTTTCGCCAGCACTATCCTAATGCGCAGCCTGTCTC
AGAGCGTGGCGACTACGGTCATCAGCTCCAATTCACGACTCCAAGTGGCAAGATTGAATTGAGCTCTCCTAGTCTCGAAA
AGATAGCCCCAGGCCGCAGTGTGATTAGCTACCGCCCCGTGACGCTAAAGCAGCCTGGTGAGCTGTACTTTATTCAAGGA
AAGTCTGCATTGCATACCAACGCAGCTACTCAAAATATCCCACTGCTTTACGACTTGCAGCCAGATAACCCTGTCTGGAT
CCATCCAGACACTGCTGCCAAACTCGGGATAAGTGATGGTGATAGCATCACCATCACTAGTCAGGTGGGAGAGGAGCAAG
GTACAGCCAAGGTAACGCTTGGGATCCGCAAAGATACCGTTTTCGCACATATGGGTTTTGGCAGTAAGAACCAAGAACTC
ACACGGGCCTTCGGTAAAGGAGTGCATTGCGGCGCTTTGTTACCCCATGCTACCGCAGCCATTACAGGCAGTAATGTTCA
TACCACGGGCGTCACTATCAGACGTGTGTAA

Upstream 100 bases:

>100_bases
ATTGGGGCAGGCGTACTATGCGTACAGCCGGCTTCTCTTCGTATAAAAAATAATTTCTTTTAAATCTGATGGTTATAAAC
GTGAAAAGAGAGGTAAGAAC

Downstream 100 bases:

>100_bases
TGTGTATTTAGTTAAACTAACAAGGAGCTACTTTACTTCAACCAAGGCAACAGTATGTGTTGGAGTAGAGTGGGGGGAAC
GCAATGAACGAAAAATATGT

Product: molybdopterin oxidoreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 756; Mature: 756

Protein sequence:

>756_residues
MLSRRDFLAKGSAFCAITVYAPGTLGAQSKILTPATPFIKEINSICEMCSTRCPITVESRLGKLFIRGNLNAKSFGGKVC
ARGTSGSKLLNDPHRLVKPIKRTGPRGSGQWQEIEWEEAYQLIAVNLNQVRDQYGPEAIAVSSKSGSLSNHAFHFGKAIG
TPNIFSHGSTCPISYAVAAKVMFGNELKLDLENSKYILNFGHNLYEGVNMSMTRGLLAAQEKGTVKVVVFEPRLSVMADK
ADEWHAIKPGTDIAVVLALSHVLIEEQLYDESFVQQYVSGFEDFATSMARYSPEWASEVCGISAGDIRRIARELAAAAPA
AIADHGHRCTFTPEEFDLRRGIYAINVLLGNIERKGGVYFAKNSKLYNKLAGEAVAPSLEKIKVEGLPTPVLPRIDLAQA
QFKYAAKAGGLYQSIIPAALQQQPYAIKAWVMSRSNPVQTVTDRTQVQKALEAMDFVVACDVYISESAAYADVVLPESTY
LERDEDISDYSAKSPAYALRQQATDTIGNTRPSWQIFKEIADAMALGQFFPWQDIADYQQRQVANNPELLPQLRKEGYLS
FGIPLLMREPDMVAKFRQHYPNAQPVSERGDYGHQLQFTTPSGKIELSSPSLEKIAPGRSVISYRPVTLKQPGELYFIQG
KSALHTNAATQNIPLLYDLQPDNPVWIHPDTAAKLGISDGDSITITSQVGEEQGTAKVTLGIRKDTVFAHMGFGSKNQEL
TRAFGKGVHCGALLPHATAAITGSNVHTTGVTIRRV

Sequences:

>Translated_756_residues
MLSRRDFLAKGSAFCAITVYAPGTLGAQSKILTPATPFIKEINSICEMCSTRCPITVESRLGKLFIRGNLNAKSFGGKVC
ARGTSGSKLLNDPHRLVKPIKRTGPRGSGQWQEIEWEEAYQLIAVNLNQVRDQYGPEAIAVSSKSGSLSNHAFHFGKAIG
TPNIFSHGSTCPISYAVAAKVMFGNELKLDLENSKYILNFGHNLYEGVNMSMTRGLLAAQEKGTVKVVVFEPRLSVMADK
ADEWHAIKPGTDIAVVLALSHVLIEEQLYDESFVQQYVSGFEDFATSMARYSPEWASEVCGISAGDIRRIARELAAAAPA
AIADHGHRCTFTPEEFDLRRGIYAINVLLGNIERKGGVYFAKNSKLYNKLAGEAVAPSLEKIKVEGLPTPVLPRIDLAQA
QFKYAAKAGGLYQSIIPAALQQQPYAIKAWVMSRSNPVQTVTDRTQVQKALEAMDFVVACDVYISESAAYADVVLPESTY
LERDEDISDYSAKSPAYALRQQATDTIGNTRPSWQIFKEIADAMALGQFFPWQDIADYQQRQVANNPELLPQLRKEGYLS
FGIPLLMREPDMVAKFRQHYPNAQPVSERGDYGHQLQFTTPSGKIELSSPSLEKIAPGRSVISYRPVTLKQPGELYFIQG
KSALHTNAATQNIPLLYDLQPDNPVWIHPDTAAKLGISDGDSITITSQVGEEQGTAKVTLGIRKDTVFAHMGFGSKNQEL
TRAFGKGVHCGALLPHATAAITGSNVHTTGVTIRRV
>Mature_756_residues
MLSRRDFLAKGSAFCAITVYAPGTLGAQSKILTPATPFIKEINSICEMCSTRCPITVESRLGKLFIRGNLNAKSFGGKVC
ARGTSGSKLLNDPHRLVKPIKRTGPRGSGQWQEIEWEEAYQLIAVNLNQVRDQYGPEAIAVSSKSGSLSNHAFHFGKAIG
TPNIFSHGSTCPISYAVAAKVMFGNELKLDLENSKYILNFGHNLYEGVNMSMTRGLLAAQEKGTVKVVVFEPRLSVMADK
ADEWHAIKPGTDIAVVLALSHVLIEEQLYDESFVQQYVSGFEDFATSMARYSPEWASEVCGISAGDIRRIARELAAAAPA
AIADHGHRCTFTPEEFDLRRGIYAINVLLGNIERKGGVYFAKNSKLYNKLAGEAVAPSLEKIKVEGLPTPVLPRIDLAQA
QFKYAAKAGGLYQSIIPAALQQQPYAIKAWVMSRSNPVQTVTDRTQVQKALEAMDFVVACDVYISESAAYADVVLPESTY
LERDEDISDYSAKSPAYALRQQATDTIGNTRPSWQIFKEIADAMALGQFFPWQDIADYQQRQVANNPELLPQLRKEGYLS
FGIPLLMREPDMVAKFRQHYPNAQPVSERGDYGHQLQFTTPSGKIELSSPSLEKIAPGRSVISYRPVTLKQPGELYFIQG
KSALHTNAATQNIPLLYDLQPDNPVWIHPDTAAKLGISDGDSITITSQVGEEQGTAKVTLGIRKDTVFAHMGFGSKNQEL
TRAFGKGVHCGALLPHATAAITGSNVHTTGVTIRRV

Specific function: Oxidoreductase which produces hydrogen sulfide from thiosulfate (Potential) [H]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasm Face Of The Membrane [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI1787870, Length=728, Percent_Identity=26.0989010989011, Blast_Score=177, Evalue=2e-45,
Organism=Escherichia coli, GI87081797, Length=741, Percent_Identity=25.7759784075574, Blast_Score=176, Evalue=5e-45,
Organism=Escherichia coli, GI171474008, Length=775, Percent_Identity=25.8064516129032, Blast_Score=170, Evalue=3e-43,
Organism=Escherichia coli, GI3868721, Length=697, Percent_Identity=24.390243902439, Blast_Score=160, Evalue=3e-40,
Organism=Escherichia coli, GI145693196, Length=679, Percent_Identity=24.7422680412371, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI87081994, Length=686, Percent_Identity=23.6151603498542, Blast_Score=113, Evalue=5e-26,
Organism=Escherichia coli, GI1787231, Length=686, Percent_Identity=21.5743440233236, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI3868719, Length=603, Percent_Identity=20.5638474295191, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655
- InterPro:   IPR006311 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: 1.8.99.- [C]

Molecular weight: Translated: 82781; Mature: 82781

Theoretical pI: Translated: 8.10; Mature: 8.10

Prosite motif: PS00490 MOLYBDOPTERIN_PROK_2 ; PS00932 MOLYBDOPTERIN_PROK_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSRRDFLAKGSAFCAITVYAPGTLGAQSKILTPATPFIKEINSICEMCSTRCPITVESR
CCCCCCHHCCCCEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCC
LGKLFIRGNLNAKSFGGKVCARGTSGSKLLNDPHRLVKPIKRTGPRGSGQWQEIEWEEAY
CCEEEEEECCCCCCCCCCEEECCCCCCHHHCCHHHHHHHHHHCCCCCCCCCCCCCHHHHH
QLIAVNLNQVRDQYGPEAIAVSSKSGSLSNHAFHFGKAIGTPNIFSHGSTCPISYAVAAK
EEEEECHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHH
VMFGNELKLDLENSKYILNFGHNLYEGVNMSMTRGLLAAQEKGTVKVVVFEPRLSVMADK
EEECCEEEEEECCCEEEEECCCHHHHCCCHHHHHHHHHCCCCCCEEEEEECCCHHHHHCC
ADEWHAIKPGTDIAVVLALSHVLIEEQLYDESFVQQYVSGFEDFATSMARYSPEWASEVC
CCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHC
GISAGDIRRIARELAAAAPAAIADHGHRCTFTPEEFDLRRGIYAINVLLGNIERKGGVYF
CCCHHHHHHHHHHHHHHCCHHHHCCCCEECCCCHHHHHHCCCEEEEEHHHCCCCCCCEEE
AKNSKLYNKLAGEAVAPSLEKIKVEGLPTPVLPRIDLAQAQFKYAAKAGGLYQSIIPAAL
EECCHHHHHHCCHHHCCCHHHEEECCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHH
QQQPYAIKAWVMSRSNPVQTVTDRTQVQKALEAMDFVVACDVYISESAAYADVVLPESTY
HCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCEEEEECCCCHH
LERDEDISDYSAKSPAYALRQQATDTIGNTRPSWQIFKEIADAMALGQFFPWQDIADYQQ
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHH
RQVANNPELLPQLRKEGYLSFGIPLLMREPDMVAKFRQHYPNAQPVSERGDYGHQLQFTT
HHHCCCCHHHHHHHHCCCEEECCEEEECCCHHHHHHHHHCCCCCCCHHCCCCCCEEEEEC
PSGKIELSSPSLEKIAPGRSVISYRPVTLKQPGELYFIQGKSALHTNAATQNIPLLYDLQ
CCCEEEECCCCHHHHCCCCCEEEECCEEECCCCCEEEEECCCCEECCCCCCCCCEEEEEC
PDNPVWIHPDTAAKLGISDGDSITITSQVGEEQGTAKVTLGIRKDTVFAHMGFGSKNQEL
CCCCEEECCCCCCEECCCCCCCEEEEECCCCCCCCEEEEEEEECCEEEEEECCCCCCHHH
TRAFGKGVHCGALLPHATAAITGSNVHTTGVTIRRV
HHHHCCCCEECCCCCCCCEEEECCCEEECCEEEEEC
>Mature Secondary Structure
MLSRRDFLAKGSAFCAITVYAPGTLGAQSKILTPATPFIKEINSICEMCSTRCPITVESR
CCCCCCHHCCCCEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCC
LGKLFIRGNLNAKSFGGKVCARGTSGSKLLNDPHRLVKPIKRTGPRGSGQWQEIEWEEAY
CCEEEEEECCCCCCCCCCEEECCCCCCHHHCCHHHHHHHHHHCCCCCCCCCCCCCHHHHH
QLIAVNLNQVRDQYGPEAIAVSSKSGSLSNHAFHFGKAIGTPNIFSHGSTCPISYAVAAK
EEEEECHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHH
VMFGNELKLDLENSKYILNFGHNLYEGVNMSMTRGLLAAQEKGTVKVVVFEPRLSVMADK
EEECCEEEEEECCCEEEEECCCHHHHCCCHHHHHHHHHCCCCCCEEEEEECCCHHHHHCC
ADEWHAIKPGTDIAVVLALSHVLIEEQLYDESFVQQYVSGFEDFATSMARYSPEWASEVC
CCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHC
GISAGDIRRIARELAAAAPAAIADHGHRCTFTPEEFDLRRGIYAINVLLGNIERKGGVYF
CCCHHHHHHHHHHHHHHCCHHHHCCCCEECCCCHHHHHHCCCEEEEEHHHCCCCCCCEEE
AKNSKLYNKLAGEAVAPSLEKIKVEGLPTPVLPRIDLAQAQFKYAAKAGGLYQSIIPAAL
EECCHHHHHHCCHHHCCCHHHEEECCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHH
QQQPYAIKAWVMSRSNPVQTVTDRTQVQKALEAMDFVVACDVYISESAAYADVVLPESTY
HCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCEEEEECCCCHH
LERDEDISDYSAKSPAYALRQQATDTIGNTRPSWQIFKEIADAMALGQFFPWQDIADYQQ
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHH
RQVANNPELLPQLRKEGYLSFGIPLLMREPDMVAKFRQHYPNAQPVSERGDYGHQLQFTT
HHHCCCCHHHHHHHHCCCEEECCEEEECCCHHHHHHHHHCCCCCCCHHCCCCCCEEEEEC
PSGKIELSSPSLEKIAPGRSVISYRPVTLKQPGELYFIQGKSALHTNAATQNIPLLYDLQ
CCCEEEECCCCHHHHCCCCCEEEECCEEECCCCCEEEEECCCCEECCCCCCCCCEEEEEC
PDNPVWIHPDTAAKLGISDGDSITITSQVGEEQGTAKVTLGIRKDTVFAHMGFGSKNQEL
CCCCEEECCCCCCEECCCCCCCEEEEECCCCCCCCEEEEEEEECCEEEEEECCCCCCHHH
TRAFGKGVHCGALLPHATAAITGSNVHTTGVTIRRV
HHHHCCCCEECCCCCCCCEEEECCCEEECCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: May Bind 4Fe-4S Cluster. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7751291; 11677609; 7737516 [H]