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The map label for this gene is psrB [H]

Identifier: 157963004

GI number: 157963004

Start: 3907475

End: 3908053

Strand: Reverse

Name: psrB [H]

Synonym: Spea_3187

Alternate gene names: 157963004

Gene position: 3908053-3907475 (Counterclockwise)

Preceding gene: 157963005

Following gene: 157962998

Centisome position: 75.52

GC content: 47.84

Gene sequence:

>579_bases
ATGAACGAAAAATATGTACTCATTCATGATGAAAATCGCTGTATAGGTTGTCATGCCTGCACTGCTGCTTGTAACACATC
TAACGATTTGGCCGATCATCAAAACCGGGCCACAATTGATGTAAAGGGCCCATATCTTATCGATGGCGAACTGCACTATC
GTTATCTGCGAGTCTCTTGCGAGCAGTGTGACAGACCCGCCTGCATCAAGGCCTGCCCTTATGGGGCGATTAGTCAAACA
GTGGATGGTGTAGTGTCATTTAATCATGACCGCTGCAAAGGCTGCTTAATGTGTGTAGCCGCCTGTCCTACAGGGGGATG
TTTCTATAATAAGCACGCCAAATCTACTGATAACTGCGATTTCTGCATGAAAAGCCGTGTTGCAGAGGGGCTACAGCCAG
CCTGCGTCACGGTATGTCCTGCCGATGCCCTCATTTTTGGCAATGCCAACGATCCAAACAGCGAAGTACATAAGTGTCTG
TCGACGGTACTCACCTATAACCGTCCAGTTGGCTATAACAATAAACCTAAGCTCTACCGAGTAGCCAGTCAGGTGGCAAG
TGAACGGAGACTTGTTTAG

Upstream 100 bases:

>100_bases
CTATCAGACGTGTGTAATGTGTATTTAGTTAAACTAACAAGGAGCTACTTTACTTCAACCAAGGCAACAGTATGTGTTGG
AGTAGAGTGGGGGGAACGCA

Downstream 100 bases:

>100_bases
CTCAACAGTTGAGGTTTTGCCACCTATCAGGCGGCGCTTGAGCTTTAAGTTAGACAGCGTCTAACAATTAGGAGGAGTAA
TCAAGCTGCGCTTGATAGCA

Product: 4Fe-4S ferredoxin iron-sulfur-binding domain-containing protein

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: Sulfur reductase chain B [H]

Number of amino acids: Translated: 192; Mature: 192

Protein sequence:

>192_residues
MNEKYVLIHDENRCIGCHACTAACNTSNDLADHQNRATIDVKGPYLIDGELHYRYLRVSCEQCDRPACIKACPYGAISQT
VDGVVSFNHDRCKGCLMCVAACPTGGCFYNKHAKSTDNCDFCMKSRVAEGLQPACVTVCPADALIFGNANDPNSEVHKCL
STVLTYNRPVGYNNKPKLYRVASQVASERRLV

Sequences:

>Translated_192_residues
MNEKYVLIHDENRCIGCHACTAACNTSNDLADHQNRATIDVKGPYLIDGELHYRYLRVSCEQCDRPACIKACPYGAISQT
VDGVVSFNHDRCKGCLMCVAACPTGGCFYNKHAKSTDNCDFCMKSRVAEGLQPACVTVCPADALIFGNANDPNSEVHKCL
STVLTYNRPVGYNNKPKLYRVASQVASERRLV
>Mature_192_residues
MNEKYVLIHDENRCIGCHACTAACNTSNDLADHQNRATIDVKGPYLIDGELHYRYLRVSCEQCDRPACIKACPYGAISQT
VDGVVSFNHDRCKGCLMCVAACPTGGCFYNKHAKSTDNCDFCMKSRVAEGLQPACVTVCPADALIFGNANDPNSEVHKCL
STVLTYNRPVGYNNKPKLYRVASQVASERRLV

Specific function: Component of the phosphorylative electron transport system with polysulfide as the terminal acceptor [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI2367345, Length=179, Percent_Identity=42.4581005586592, Blast_Score=149, Evalue=1e-37,
Organism=Escherichia coli, GI226510944, Length=181, Percent_Identity=37.5690607734807, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1787872, Length=185, Percent_Identity=36.7567567567568, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1787122, Length=158, Percent_Identity=37.3417721518987, Blast_Score=97, Evalue=6e-22,
Organism=Escherichia coli, GI1787749, Length=158, Percent_Identity=34.8101265822785, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1790326, Length=163, Percent_Identity=32.5153374233129, Blast_Score=84, Evalue=5e-18,
Organism=Escherichia coli, GI1789370, Length=180, Percent_Identity=28.8888888888889, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1787963, Length=151, Percent_Identity=29.8013245033113, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI87082179, Length=145, Percent_Identity=35.8620689655172, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1788811, Length=145, Percent_Identity=32.4137931034483, Blast_Score=68, Evalue=4e-13,
Organism=Escherichia coli, GI1787478, Length=86, Percent_Identity=36.046511627907, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1787740, Length=86, Percent_Identity=33.7209302325581, Blast_Score=63, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 21106; Mature: 21106

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

9.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
10.9 %Cys+Met (Translated Protein)
9.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
10.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEKYVLIHDENRCIGCHACTAACNTSNDLADHQNRATIDVKGPYLIDGELHYRYLRVSC
CCCCEEEEECCCCEEEHHHHHHHCCCCCCHHCCCCCEEEEECCCEEECCCEEEEEEEEEH
EQCDRPACIKACPYGAISQTVDGVVSFNHDRCKGCLMCVAACPTGGCFYNKHAKSTDNCD
HHCCCCCHHHHCCCCHHHHHHHHHEECCCHHHHHHHHHHHHCCCCCEEECCCCCCCCCHH
FCMKSRVAEGLQPACVTVCPADALIFGNANDPNSEVHKCLSTVLTYNRPVGYNNKPKLYR
HHHHHHHHCCCCCCEEEECCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHH
VASQVASERRLV
HHHHHHHHCCCC
>Mature Secondary Structure
MNEKYVLIHDENRCIGCHACTAACNTSNDLADHQNRATIDVKGPYLIDGELHYRYLRVSC
CCCCEEEEECCCCEEEHHHHHHHCCCCCCHHCCCCCEEEEECCCEEECCCEEEEEEEEEH
EQCDRPACIKACPYGAISQTVDGVVSFNHDRCKGCLMCVAACPTGGCFYNKHAKSTDNCD
HHCCCCCHHHHCCCCHHHHHHHHHEECCCHHHHHHHHHHHHCCCCCEEECCCCCCCCCHH
FCMKSRVAEGLQPACVTVCPADALIFGNANDPNSEVHKCLSTVLTYNRPVGYNNKPKLYR
HHHHHHHHCCCCCCEEEECCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHH
VASQVASERRLV
HHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1597189; 14500908 [H]