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The map label for this gene is clpB [H]

Identifier: 157962981

GI number: 157962981

Start: 3874215

End: 3876788

Strand: Reverse

Name: clpB [H]

Synonym: Spea_3164

Alternate gene names: 157962981

Gene position: 3876788-3874215 (Counterclockwise)

Preceding gene: 157962982

Following gene: 157962980

Centisome position: 74.92

GC content: 46.11

Gene sequence:

>2574_bases
ATGCGACTCGATCGTATGACCAATAAATTCCAAATTGCCATCTCGGATGCTCAGTCATTGGCACTAGGCCGAGATCATCA
ATTTATCGAACCATCACATTTGATGATGGCGTTACTGAATCAAGATGGTGGTTCAATTCACCCTCTACTCACGCAAGCAG
GGATCCAAGTAAGTACTCTGCGCTCGTTATTGAGTAAAGATCTAGAACAAAGACCTCAAGTCGAAGGGACTGGCGGGGAT
GTACAGCTATCTCAGGCATTGATTCGCCTACTCAATTTATGCGATAAGCTGGCGCAAAAGCGTAAAGACAAGTTTATCTC
TAGTGAGCTGTTTGTATTAGCGGCGCTAGAGGGGAATGACAGCCTAGCGCAAGCACTAAAACAATCCGGTGCGACTAAAG
AGTTGATGGAGTCGACTATAGACCAAATTCGTAACGGACAAAATGTGGACGACCCTAATGCTGAAGATCAGCGTCAAGCA
CTTAAGAAGTTTACCGTCGATCTTACCGAGCGAGCAGAGCAGGGGAAATTAGACCCAGTTATTGGTCGTGACGATGAAAT
TCGCCGTACCATTCAAGTGTTGCAGCGCCGTAGCAAGAACAATCCTGTATTGATCGGAGAGCCGGGTGTCGGTAAAACGG
CGATTGTTGAAGGCTTAGCGCAGCGGATCATTAACAACGAAGTCCCCGAAGGCATCAAGAATAAGCGTGTATTGTCATTG
GACATGGGCGCATTAGTGGCAGGTGCTAAGTATCGCGGTGAATTTGAGGAGCGTTTAAAAGCGGTGCTCAATGAGTTGTC
TCAAGAGGAGGGCCAAGTGATCCTCTTTATTGATGAACTACACACTATGGTAGGTGCGGGTAAGTCTGATGGCGCGATGG
ATGCGGGCAATATGTTAAAGCCTGCGCTGGCACGCGGTGATCTACACTGCGTGGGCGCAACAACATTGGATGAATATCGT
CAGTACATTGAAAAAGATGCAGCGCTTGAGCGTCGTTTCCAGAAAGTGCTGGTAGATGAGCCGAGTGTTGAAGACACTAT
CGCTATCTTACGTGGTCTGAAAGAGCGCTATGAGCTGCATCATCACGTTGAAATTACCGATCCGGCGATTGTTGCTGCGG
CAAGTATGTCACATCGCTATATCTCAGATCGTAAGTTGCCAGATAAAGCTATCGATCTGATTGACGAGGCTGCATCGAGC
ATCCGTATTCAGATTGACTCTAAGCCGGAATCTTTAGATAGATTGGAACGACGTGCTATTCAGCTAAAGCTTGAAGAGCA
AGCGTTAAGTAAAGAAGCTGATGAAGCCAGCTTACGCCGTTTAGACACATTGCGTGCTGAGCTTAAAGATGTTGAAGCGA
AAGGGGCTGAGCTAAATGAGATTTGGCATACCGAGAAAGCAGCGTTAGCTGGTACTCAGCATATAAAAGCTGATTTAGAG
CAAGCACGTATGGATCTTGAAGTGGCTAGACGTGCGGGTGACTTAACTCGTATGTCAGAGCTGCAGTATGGACGTATTCC
AGAGCTTGAAAAGCAGCTAGATTTAGCGGCGCAAGCTGAAATGCAAGATATGACCTTATTGCGGAATAAGGTTACCGATG
TTGAGATTGCCGAAGTTTTATCAAAGGCTACAGGTATTCCGGTATCTAAGATGCTCGAAGGGGAACGTGACAAGCTGCTT
AAGATGGAAGATGCGCTGCACGAACGAGTTATCGGCCAAAACGAAGCAGTAGATTCTGTGTCTAATGCAATTCGTCGTAG
CCGAGCTGGTCTTGCAGATCCGAATCGTCCAATTGGCTCTTTTTTATTCTTAGGTCCAACAGGCGTTGGTAAAACTGAGC
TATGTAAGTCGCTTGCTAAATTCTTGTTCGATACCGAATCTGCACTCGTTCGAGTCGACATGTCTGAGTTTATGGAAAAA
CATTCGGTTTCACGATTAGTTGGTGCCCCTCCAGGATATGTGGGTTATGAAGAGGGCGGCTACTTAACGGAAGCTGTGAG
ACGTAAACCATATTCGGTGATCCTACTTGATGAAGTTGAGAAGGCGCATCCAGATGTCTTTAATATCTTACTGCAAGTAT
TAGATGATGGACGCCTAACCGATGGACAAGGGCGCACGGTAGACTTTAGAAATACAGTGGTGATCATGACCTCTAATCTA
GGTTCAGATCTCATCCAATTGGGCTTTGGTACTCAAAGCTATGATGAGATGAAGTCTGATGTGATGCAGGTGGTGATGCA
GAACTTCAGACCTGAGTTTTTGAACCGTGTCGATGAGGCGGTAGTGTTCCATCCACTTGATGAGAAGAATATTGCCCATA
TTGCCAAGATCCAAATTGAAGGCCTTAAAAAACGCCTTGCAGACAAGGATTATGGACTTGAGATGAGTGATGACGCTCTA
GCATTGATTGCTCGAGCGGGTTTTGATCCTGTATATGGTGCAAGACCACTTAAGAGAGCATTGCAGCAAGAGGTTGAAAA
TCCATTGGCACAGCAGATCCTCAGTGGTCAGCTCTTGCCTGGCAAGGATATTAAGGTTTCAGTTGAAAACGACACCTTAG
TGTTTAGCCAATAA

Upstream 100 bases:

>100_bases
TCATAAATGGCGGTATGAATGCTATCCAATTCGGTTTCTCGAAGCAGATATGCAATGCCCATTGCCTTTTAATCACTATT
TTTGAGTTATCGGAGGATTT

Downstream 100 bases:

>100_bases
TGTGAGTTAAAACAGCAAGCCGAACAGGCCGAGTGTTTAATATAAAGCTCAATAAAAGGTTTCAACATTTGTTGAAGCCT
TTTTTGTATAAAAATTGCGG

Product: ATPase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 857; Mature: 857

Protein sequence:

>857_residues
MRLDRMTNKFQIAISDAQSLALGRDHQFIEPSHLMMALLNQDGGSIHPLLTQAGIQVSTLRSLLSKDLEQRPQVEGTGGD
VQLSQALIRLLNLCDKLAQKRKDKFISSELFVLAALEGNDSLAQALKQSGATKELMESTIDQIRNGQNVDDPNAEDQRQA
LKKFTVDLTERAEQGKLDPVIGRDDEIRRTIQVLQRRSKNNPVLIGEPGVGKTAIVEGLAQRIINNEVPEGIKNKRVLSL
DMGALVAGAKYRGEFEERLKAVLNELSQEEGQVILFIDELHTMVGAGKSDGAMDAGNMLKPALARGDLHCVGATTLDEYR
QYIEKDAALERRFQKVLVDEPSVEDTIAILRGLKERYELHHHVEITDPAIVAAASMSHRYISDRKLPDKAIDLIDEAASS
IRIQIDSKPESLDRLERRAIQLKLEEQALSKEADEASLRRLDTLRAELKDVEAKGAELNEIWHTEKAALAGTQHIKADLE
QARMDLEVARRAGDLTRMSELQYGRIPELEKQLDLAAQAEMQDMTLLRNKVTDVEIAEVLSKATGIPVSKMLEGERDKLL
KMEDALHERVIGQNEAVDSVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKSLAKFLFDTESALVRVDMSEFMEK
HSVSRLVGAPPGYVGYEEGGYLTEAVRRKPYSVILLDEVEKAHPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNL
GSDLIQLGFGTQSYDEMKSDVMQVVMQNFRPEFLNRVDEAVVFHPLDEKNIAHIAKIQIEGLKKRLADKDYGLEMSDDAL
ALIARAGFDPVYGARPLKRALQQEVENPLAQQILSGQLLPGKDIKVSVENDTLVFSQ

Sequences:

>Translated_857_residues
MRLDRMTNKFQIAISDAQSLALGRDHQFIEPSHLMMALLNQDGGSIHPLLTQAGIQVSTLRSLLSKDLEQRPQVEGTGGD
VQLSQALIRLLNLCDKLAQKRKDKFISSELFVLAALEGNDSLAQALKQSGATKELMESTIDQIRNGQNVDDPNAEDQRQA
LKKFTVDLTERAEQGKLDPVIGRDDEIRRTIQVLQRRSKNNPVLIGEPGVGKTAIVEGLAQRIINNEVPEGIKNKRVLSL
DMGALVAGAKYRGEFEERLKAVLNELSQEEGQVILFIDELHTMVGAGKSDGAMDAGNMLKPALARGDLHCVGATTLDEYR
QYIEKDAALERRFQKVLVDEPSVEDTIAILRGLKERYELHHHVEITDPAIVAAASMSHRYISDRKLPDKAIDLIDEAASS
IRIQIDSKPESLDRLERRAIQLKLEEQALSKEADEASLRRLDTLRAELKDVEAKGAELNEIWHTEKAALAGTQHIKADLE
QARMDLEVARRAGDLTRMSELQYGRIPELEKQLDLAAQAEMQDMTLLRNKVTDVEIAEVLSKATGIPVSKMLEGERDKLL
KMEDALHERVIGQNEAVDSVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKSLAKFLFDTESALVRVDMSEFMEK
HSVSRLVGAPPGYVGYEEGGYLTEAVRRKPYSVILLDEVEKAHPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNL
GSDLIQLGFGTQSYDEMKSDVMQVVMQNFRPEFLNRVDEAVVFHPLDEKNIAHIAKIQIEGLKKRLADKDYGLEMSDDAL
ALIARAGFDPVYGARPLKRALQQEVENPLAQQILSGQLLPGKDIKVSVENDTLVFSQ
>Mature_857_residues
MRLDRMTNKFQIAISDAQSLALGRDHQFIEPSHLMMALLNQDGGSIHPLLTQAGIQVSTLRSLLSKDLEQRPQVEGTGGD
VQLSQALIRLLNLCDKLAQKRKDKFISSELFVLAALEGNDSLAQALKQSGATKELMESTIDQIRNGQNVDDPNAEDQRQA
LKKFTVDLTERAEQGKLDPVIGRDDEIRRTIQVLQRRSKNNPVLIGEPGVGKTAIVEGLAQRIINNEVPEGIKNKRVLSL
DMGALVAGAKYRGEFEERLKAVLNELSQEEGQVILFIDELHTMVGAGKSDGAMDAGNMLKPALARGDLHCVGATTLDEYR
QYIEKDAALERRFQKVLVDEPSVEDTIAILRGLKERYELHHHVEITDPAIVAAASMSHRYISDRKLPDKAIDLIDEAASS
IRIQIDSKPESLDRLERRAIQLKLEEQALSKEADEASLRRLDTLRAELKDVEAKGAELNEIWHTEKAALAGTQHIKADLE
QARMDLEVARRAGDLTRMSELQYGRIPELEKQLDLAAQAEMQDMTLLRNKVTDVEIAEVLSKATGIPVSKMLEGERDKLL
KMEDALHERVIGQNEAVDSVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKSLAKFLFDTESALVRVDMSEFMEK
HSVSRLVGAPPGYVGYEEGGYLTEAVRRKPYSVILLDEVEKAHPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNL
GSDLIQLGFGTQSYDEMKSDVMQVVMQNFRPEFLNRVDEAVVFHPLDEKNIAHIAKIQIEGLKKRLADKDYGLEMSDDAL
ALIARAGFDPVYGARPLKRALQQEVENPLAQQILSGQLLPGKDIKVSVENDTLVFSQ

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=320, Percent_Identity=36.25, Blast_Score=209, Evalue=9e-54,
Organism=Escherichia coli, GI1788943, Length=857, Percent_Identity=77.0128354725788, Blast_Score=1352, Evalue=0.0,
Organism=Escherichia coli, GI1787109, Length=420, Percent_Identity=39.0476190476191, Blast_Score=278, Evalue=1e-75,
Organism=Saccharomyces cerevisiae, GI6320464, Length=712, Percent_Identity=52.8089887640449, Blast_Score=767, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6323002, Length=843, Percent_Identity=42.8232502965599, Blast_Score=658, Evalue=0.0,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR017730
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 95234; Mature: 95234

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00870 CLPAB_1 ; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLDRMTNKFQIAISDAQSLALGRDHQFIEPSHLMMALLNQDGGSIHPLLTQAGIQVSTL
CCCCCCCCEEEEEECCHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHCCCHHHHH
RSLLSKDLEQRPQVEGTGGDVQLSQALIRLLNLCDKLAQKRKDKFISSELFVLAALEGND
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCH
SLAQALKQSGATKELMESTIDQIRNGQNVDDPNAEDQRQALKKFTVDLTERAEQGKLDPV
HHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
IGRDDEIRRTIQVLQRRSKNNPVLIGEPGVGKTAIVEGLAQRIINNEVPEGIKNKRVLSL
CCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEE
DMGALVAGAKYRGEFEERLKAVLNELSQEEGQVILFIDELHTMVGAGKSDGAMDAGNMLK
EHHHHHHCCHHCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCCCCCCCCHHH
PALARGDLHCVGATTLDEYRQYIEKDAALERRFQKVLVDEPSVEDTIAILRGLKERYELH
HHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
HHVEITDPAIVAAASMSHRYISDRKLPDKAIDLIDEAASSIRIQIDSKPESLDRLERRAI
HCEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHH
QLKLEEQALSKEADEASLRRLDTLRAELKDVEAKGAELNEIWHTEKAALAGTQHIKADLE
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
QARMDLEVARRAGDLTRMSELQYGRIPELEKQLDLAAQAEMQDMTLLRNKVTDVEIAEVL
HHHHHHHHHHHHCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
SKATGIPVSKMLEGERDKLLKMEDALHERVIGQNEAVDSVSNAIRRSRAGLADPNRPIGS
HHHCCCCHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
FLFLGPTGVGKTELCKSLAKFLFDTESALVRVDMSEFMEKHSVSRLVGAPPGYVGYEEGG
EEEECCCCCCHHHHHHHHHHHHHCCCHHHEEECHHHHHHHHHHHHHHCCCCCCCCCCCCC
YLTEAVRRKPYSVILLDEVEKAHPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNL
HHHHHHHCCCCEEEEECCHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEEECCC
GSDLIQLGFGTQSYDEMKSDVMQVVMQNFRPEFLNRVDEAVVFHPLDEKNIAHIAKIQIE
CHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHEEECCCCCCCCHHHHHHHHH
GLKKRLADKDYGLEMSDDALALIARAGFDPVYGARPLKRALQQEVENPLAQQILSGQLLP
HHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCHHHHHHHCCCCCC
GKDIKVSVENDTLVFSQ
CCCCEEEECCCEEEECC
>Mature Secondary Structure
MRLDRMTNKFQIAISDAQSLALGRDHQFIEPSHLMMALLNQDGGSIHPLLTQAGIQVSTL
CCCCCCCCEEEEEECCHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHCCCHHHHH
RSLLSKDLEQRPQVEGTGGDVQLSQALIRLLNLCDKLAQKRKDKFISSELFVLAALEGND
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCH
SLAQALKQSGATKELMESTIDQIRNGQNVDDPNAEDQRQALKKFTVDLTERAEQGKLDPV
HHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
IGRDDEIRRTIQVLQRRSKNNPVLIGEPGVGKTAIVEGLAQRIINNEVPEGIKNKRVLSL
CCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEE
DMGALVAGAKYRGEFEERLKAVLNELSQEEGQVILFIDELHTMVGAGKSDGAMDAGNMLK
EHHHHHHCCHHCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCCCCCCCCHHH
PALARGDLHCVGATTLDEYRQYIEKDAALERRFQKVLVDEPSVEDTIAILRGLKERYELH
HHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
HHVEITDPAIVAAASMSHRYISDRKLPDKAIDLIDEAASSIRIQIDSKPESLDRLERRAI
HCEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHH
QLKLEEQALSKEADEASLRRLDTLRAELKDVEAKGAELNEIWHTEKAALAGTQHIKADLE
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
QARMDLEVARRAGDLTRMSELQYGRIPELEKQLDLAAQAEMQDMTLLRNKVTDVEIAEVL
HHHHHHHHHHHHCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
SKATGIPVSKMLEGERDKLLKMEDALHERVIGQNEAVDSVSNAIRRSRAGLADPNRPIGS
HHHCCCCHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
FLFLGPTGVGKTELCKSLAKFLFDTESALVRVDMSEFMEKHSVSRLVGAPPGYVGYEEGG
EEEECCCCCCHHHHHHHHHHHHHCCCHHHEEECHHHHHHHHHHHHHHCCCCCCCCCCCCC
YLTEAVRRKPYSVILLDEVEKAHPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNL
HHHHHHHCCCCEEEEECCHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEEECCC
GSDLIQLGFGTQSYDEMKSDVMQVVMQNFRPEFLNRVDEAVVFHPLDEKNIAHIAKIQIE
CHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHEEECCCCCCCCHHHHHHHHH
GLKKRLADKDYGLEMSDDALALIARAGFDPVYGARPLKRALQQEVENPLAQQILSGQLLP
HHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCHHHHHHHCCCCCC
GKDIKVSVENDTLVFSQ
CCCCEEEECCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]