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The map label for this gene is ubiF [H]

Identifier: 157962952

GI number: 157962952

Start: 3839549

End: 3840748

Strand: Reverse

Name: ubiF [H]

Synonym: Spea_3135

Alternate gene names: 157962952

Gene position: 3840748-3839549 (Counterclockwise)

Preceding gene: 157962959

Following gene: 157962951

Centisome position: 74.22

GC content: 47.33

Gene sequence:

>1200_bases
GTGGAACAGACAAGTAGTAAATCGAGTCTATATGATGCGGTGGTTGTCGGTGGCGGCATGGTCGGCGCCGCTGCAGCGAT
AGGTCTTGGGCAGCTAGGATTAAAGGTGGCAGTAGTCGAAGCGTTTGCTCCAAAGGCTTATGACAGCGAACAGCCACTGG
ATTTGCGTGTATCCGCTATCAGCGCGGCATCGGAAGCTCTGCTTGAGCGCCTTGGGGCCTTGGGTGGACTCGACTCGATG
CGTAAGGTTGCCTATAAAGGCCTGGAAACGTGGGAGATGGATGGCTGTATCACCCGTTTTCATAGCGACCAGATTGGCAC
CTCTCATTTAGGTCATATCGTGGAGAATCGTCTGATCCAACTTTCTCTTTGGCAGCGTATGCAGCAGCTAGAGAATATTT
CTCTGTTCTGCCCGGTTAAAATTTCCGATATGTCACGTGACCCACTTCAAGATTTAATTGAAGTGCAGCTCGATAATGGG
GATGCATTGCAGGCTCGTTTGTTAATTGGCGCCGATGGCGCGAACTCTATGGTGCGACAGTGGGCTAATATAGGCATTAC
GGCTTGGGACTATGCTCAATCGGCGATGCTGATTAATATTGAAACCGCCTGTGCAGAGCAAGATGTTACCTGGCAACAGT
TCACACCAAGCGGGCCGCGATCGTTATTACCATTGCCAGATAACAATGCTTCATTAGTTTGGTATGACGACGCCAACAGA
ATTGCTCAGCTGAGCCAACTCAACAATTCTGCATTAAAGACGCAGATTGACAAATATTTTCCAGAGCGGTTAGACAGAAA
CTTCAGCGTTGTTGACAAGGCAAGTTTTAAGCTGACTCGCCGACATGCGCAAACTTACTACCGAGATAACTTGGTGATCT
TAGGCGACGCAGCCCATACAATTAATCCACTCGCAGGGCAGGGCGTCAATTTAGGTTTTAAAGATGTCGACGCCTTGATT
AATACCATAGCTGGACATCTAGAAGAGACAAACGGCTGGTGGAATTCTCAAGTATTAGCTAAGTATCAGCGAAGCCGGTA
CCGTGATAATCAATTGATGATGTCGACCATGGATTTGTTCTACGCAAGTTTCAGTAATGACATTCTGCCACTTAAGCTTC
TGCGTAATGGCGCACTAAAACTTGCTAATATTGATAGTCCAATTAAAAAGCAGGTACTTAGGTACGCAATGGGCTTTTAA

Upstream 100 bases:

>100_bases
AAATAGTGCAAACTTTCTTATATAAAGCCTCCAATCACGACGCTCTTTAGGTGTAAACTCTCAAGCAATAGTGATTGCAG
TTGTCAGGAGACAGGTAGCA

Downstream 100 bases:

>100_bases
GATAGTGCCTATTTAAAGATTAAAAACCGAGTGAAAAACACTCGGTTTTTTTCGTTACGGCTAAGCAAAACCACTAATTT
TTATCGGATAACTCTACTGG

Product: UbiH/UbiF/VisC/COQ6 family ubiquinone biosynthesis hydroxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MEQTSSKSSLYDAVVVGGGMVGAAAAIGLGQLGLKVAVVEAFAPKAYDSEQPLDLRVSAISAASEALLERLGALGGLDSM
RKVAYKGLETWEMDGCITRFHSDQIGTSHLGHIVENRLIQLSLWQRMQQLENISLFCPVKISDMSRDPLQDLIEVQLDNG
DALQARLLIGADGANSMVRQWANIGITAWDYAQSAMLINIETACAEQDVTWQQFTPSGPRSLLPLPDNNASLVWYDDANR
IAQLSQLNNSALKTQIDKYFPERLDRNFSVVDKASFKLTRRHAQTYYRDNLVILGDAAHTINPLAGQGVNLGFKDVDALI
NTIAGHLEETNGWWNSQVLAKYQRSRYRDNQLMMSTMDLFYASFSNDILPLKLLRNGALKLANIDSPIKKQVLRYAMGF

Sequences:

>Translated_399_residues
MEQTSSKSSLYDAVVVGGGMVGAAAAIGLGQLGLKVAVVEAFAPKAYDSEQPLDLRVSAISAASEALLERLGALGGLDSM
RKVAYKGLETWEMDGCITRFHSDQIGTSHLGHIVENRLIQLSLWQRMQQLENISLFCPVKISDMSRDPLQDLIEVQLDNG
DALQARLLIGADGANSMVRQWANIGITAWDYAQSAMLINIETACAEQDVTWQQFTPSGPRSLLPLPDNNASLVWYDDANR
IAQLSQLNNSALKTQIDKYFPERLDRNFSVVDKASFKLTRRHAQTYYRDNLVILGDAAHTINPLAGQGVNLGFKDVDALI
NTIAGHLEETNGWWNSQVLAKYQRSRYRDNQLMMSTMDLFYASFSNDILPLKLLRNGALKLANIDSPIKKQVLRYAMGF
>Mature_399_residues
MEQTSSKSSLYDAVVVGGGMVGAAAAIGLGQLGLKVAVVEAFAPKAYDSEQPLDLRVSAISAASEALLERLGALGGLDSM
RKVAYKGLETWEMDGCITRFHSDQIGTSHLGHIVENRLIQLSLWQRMQQLENISLFCPVKISDMSRDPLQDLIEVQLDNG
DALQARLLIGADGANSMVRQWANIGITAWDYAQSAMLINIETACAEQDVTWQQFTPSGPRSLLPLPDNNASLVWYDDANR
IAQLSQLNNSALKTQIDKYFPERLDRNFSVVDKASFKLTRRHAQTYYRDNLVILGDAAHTINPLAGQGVNLGFKDVDALI
NTIAGHLEETNGWWNSQVLAKYQRSRYRDNQLMMSTMDLFYASFSNDILPLKLLRNGALKLANIDSPIKKQVLRYAMGF

Specific function: Oxygenase that introduces the hydroxyl group at carbon five of 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol resulting in the formation of 2-octaprenyl-3-methyl-5-hydroxy-6-methoxy-1,4- benzoquinol [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiH/COQ6 family [H]

Homologues:

Organism=Homo sapiens, GI32967305, Length=449, Percent_Identity=32.5167037861915, Blast_Score=178, Evalue=9e-45,
Organism=Homo sapiens, GI32967307, Length=336, Percent_Identity=32.4404761904762, Blast_Score=156, Evalue=4e-38,
Organism=Escherichia coli, GI1786883, Length=388, Percent_Identity=46.1340206185567, Blast_Score=345, Evalue=2e-96,
Organism=Escherichia coli, GI1789273, Length=391, Percent_Identity=38.8746803069054, Blast_Score=238, Evalue=5e-64,
Organism=Escherichia coli, GI1789274, Length=390, Percent_Identity=26.9230769230769, Blast_Score=161, Evalue=6e-41,
Organism=Caenorhabditis elegans, GI25146631, Length=441, Percent_Identity=31.0657596371882, Blast_Score=175, Evalue=4e-44,
Organism=Saccharomyces cerevisiae, GI6321694, Length=458, Percent_Identity=27.9475982532751, Blast_Score=137, Evalue=2e-33,
Organism=Drosophila melanogaster, GI19920760, Length=436, Percent_Identity=29.8165137614679, Blast_Score=161, Evalue=9e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042
- InterPro:   IPR018168
- InterPro:   IPR010971 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.-

Molecular weight: Translated: 44186; Mature: 44186

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQTSSKSSLYDAVVVGGGMVGAAAAIGLGQLGLKVAVVEAFAPKAYDSEQPLDLRVSAI
CCCCCCHHHHHHHHEECCCHHHHHHHHHHHHHHHHEEEHHHHCCCCCCCCCCCEEEHHHH
SAASEALLERLGALGGLDSMRKVAYKGLETWEMDGCITRFHSDQIGTSHLGHIVENRLIQ
HHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEECCHHHHHHCCCCCCHHHHHHHHHHHHHH
LSLWQRMQQLENISLFCPVKISDMSRDPLQDLIEVQLDNGDALQARLLIGADGANSMVRQ
HHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHEEECCCCCEEEEEEEEECCCHHHHHHH
WANIGITAWDYAQSAMLINIETACAEQDVTWQQFTPSGPRSLLPLPDNNASLVWYDDANR
HHHCCCEEECCCCCEEEEEEEHHHCCCCCCHHHCCCCCCCCCCCCCCCCCEEEEECCHHH
IAQLSQLNNSALKTQIDKYFPERLDRNFSVVDKASFKLTRRHAQTYYRDNLVILGDAAHT
HHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHCCEEEEECCHHH
INPLAGQGVNLGFKDVDALINTIAGHLEETNGWWNSQVLAKYQRSRYRDNQLMMSTMDLF
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHH
YASFSNDILPLKLLRNGALKLANIDSPIKKQVLRYAMGF
HHHHCCCCEEHHHHHCCCEEEECCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MEQTSSKSSLYDAVVVGGGMVGAAAAIGLGQLGLKVAVVEAFAPKAYDSEQPLDLRVSAI
CCCCCCHHHHHHHHEECCCHHHHHHHHHHHHHHHHEEEHHHHCCCCCCCCCCCEEEHHHH
SAASEALLERLGALGGLDSMRKVAYKGLETWEMDGCITRFHSDQIGTSHLGHIVENRLIQ
HHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEECCHHHHHHCCCCCCHHHHHHHHHHHHHH
LSLWQRMQQLENISLFCPVKISDMSRDPLQDLIEVQLDNGDALQARLLIGADGANSMVRQ
HHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHEEECCCCCEEEEEEEEECCCHHHHHHH
WANIGITAWDYAQSAMLINIETACAEQDVTWQQFTPSGPRSLLPLPDNNASLVWYDDANR
HHHCCCEEECCCCCEEEEEEEHHHCCCCCCHHHCCCCCCCCCCCCCCCCCEEEEECCHHH
IAQLSQLNNSALKTQIDKYFPERLDRNFSVVDKASFKLTRRHAQTYYRDNLVILGDAAHT
HHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHCCEEEEECCHHH
INPLAGQGVNLGFKDVDALINTIAGHLEETNGWWNSQVLAKYQRSRYRDNQLMMSTMDLF
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHH
YASFSNDILPLKLLRNGALKLANIDSPIKKQVLRYAMGF
HHHHCCCCEEHHHHHCCCEEEECCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydroxylation [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10802164 [H]