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The map label for this gene is engD [H]

Identifier: 157962950

GI number: 157962950

Start: 3837555

End: 3838646

Strand: Reverse

Name: engD [H]

Synonym: Spea_3133

Alternate gene names: 157962950

Gene position: 3838646-3837555 (Counterclockwise)

Preceding gene: 157962951

Following gene: 157962949

Centisome position: 74.18

GC content: 45.05

Gene sequence:

>1092_bases
ATGGGTTTCAAATGTGGCATCGTAGGCCTGCCAAACGTTGGTAAATCAACACTTTTCAACGCACTAACTAAAGCTGGTAT
CGAAGCGTCAAACTTCCCGTTCTGTACAATTGAGCCTAATACAGGTGTTGTTCCTGTACCTGACACTCGTCTAGACGCAT
TGGCTGCAATTGTTAAGCCTGAGCGTGTATTGGCGACAACTATGGAGTTCGTTGACATCGCGGGTCTAGTTGCCGGTGCA
TCTAAAGGTGAAGGTCTAGGTAACAAGTTCCTAGCTAACATCCGTGAAACCGATGCTATTGGTCATGTGGTTCGTTGTTT
TGAAGATGACAACATTGTTCACGTTGCTAATAAAGTAGATCCAGCAAGTGATATCGAAGTGATTAACACTGAGCTAGCGT
TAGCTGACCTTGACTCATGTGAGCGTGCTATTACACGTCAAGCTAAACGTGCTAAAGGCGGCGACACTGAAGCTAAGTTT
GAAGTGGCCGTACTTGAGAAAATGCGTCCAGTATTAGACGAAGGCAAAATGCTACGTTCTATGGAGCTTACTAAAGAAGA
GTTAGCAGCCATTGGCTACCTAAACTTTTTAACGCTTAAGCCTACCATGTATATTGCTAACGTGGCTGAAGATGGTTTTG
AGAATAACCCACACTTAGATACTGTTAAAGCACTTGCTGCAGAAGAAAACGCAGTCGTTGTTGCAGTTTGTGCAGCCATT
GAATCTGAGCTTGCTGAAATGGACGCGGAAGACCGTGAAGAGTTCATGGCAGATCTTGGTCTAGAAGAGCCTGGTCTTGA
TCGCGTGATCCGTGCAGGTTACGATCTACTAAGTCTACAAACCTATTTCACTGCTGGTGTAAAAGAAGTACGTGCTTGGA
CTGTAAGAATTGGCGCGAGTGCACCACAGGCAGCCGGTGTTATCCATACTGACTTTGAACGTGGCTTTATCCGTGCTCAA
GTTATGGCTTACGACGACTTCATTACTTATAAGGGTGAAGCCGGCGCGAAAGAGGCGGGTAAGTTACGCGTAGAAGGTAA
GACCTATACCGTAAAAGATGGCGATGTAATGCATTTCCTATTTAACGTATAA

Upstream 100 bases:

>100_bases
AAGATATGGCAAAGGCGATGCATAGACTGCATTCTTTTAAAGCATAAACAAGTGCTTGGAGCAAAGCTTCAAGTCATTCA
TTGATATTAAAGGTAACAAT

Downstream 100 bases:

>100_bases
GCCTGACGGTACGTTAAAGCAAACGGCGACTTATTATAGTCGCCGTTTTTGTAGCTGCGATTCGAGCAAGCTGAGGGCTT
TTAGATTACCTTATAATGAT

Product: GTP-dependent nucleic acid-binding protein EngD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 363; Mature: 362

Protein sequence:

>363_residues
MGFKCGIVGLPNVGKSTLFNALTKAGIEASNFPFCTIEPNTGVVPVPDTRLDALAAIVKPERVLATTMEFVDIAGLVAGA
SKGEGLGNKFLANIRETDAIGHVVRCFEDDNIVHVANKVDPASDIEVINTELALADLDSCERAITRQAKRAKGGDTEAKF
EVAVLEKMRPVLDEGKMLRSMELTKEELAAIGYLNFLTLKPTMYIANVAEDGFENNPHLDTVKALAAEENAVVVAVCAAI
ESELAEMDAEDREEFMADLGLEEPGLDRVIRAGYDLLSLQTYFTAGVKEVRAWTVRIGASAPQAAGVIHTDFERGFIRAQ
VMAYDDFITYKGEAGAKEAGKLRVEGKTYTVKDGDVMHFLFNV

Sequences:

>Translated_363_residues
MGFKCGIVGLPNVGKSTLFNALTKAGIEASNFPFCTIEPNTGVVPVPDTRLDALAAIVKPERVLATTMEFVDIAGLVAGA
SKGEGLGNKFLANIRETDAIGHVVRCFEDDNIVHVANKVDPASDIEVINTELALADLDSCERAITRQAKRAKGGDTEAKF
EVAVLEKMRPVLDEGKMLRSMELTKEELAAIGYLNFLTLKPTMYIANVAEDGFENNPHLDTVKALAAEENAVVVAVCAAI
ESELAEMDAEDREEFMADLGLEEPGLDRVIRAGYDLLSLQTYFTAGVKEVRAWTVRIGASAPQAAGVIHTDFERGFIRAQ
VMAYDDFITYKGEAGAKEAGKLRVEGKTYTVKDGDVMHFLFNV
>Mature_362_residues
GFKCGIVGLPNVGKSTLFNALTKAGIEASNFPFCTIEPNTGVVPVPDTRLDALAAIVKPERVLATTMEFVDIAGLVAGAS
KGEGLGNKFLANIRETDAIGHVVRCFEDDNIVHVANKVDPASDIEVINTELALADLDSCERAITRQAKRAKGGDTEAKFE
VAVLEKMRPVLDEGKMLRSMELTKEELAAIGYLNFLTLKPTMYIANVAEDGFENNPHLDTVKALAAEENAVVVAVCAAIE
SELAEMDAEDREEFMADLGLEEPGLDRVIRAGYDLLSLQTYFTAGVKEVRAWTVRIGASAPQAAGVIHTDFERGFIRAQV
MAYDDFITYKGEAGAKEAGKLRVEGKTYTVKDGDVMHFLFNV

Specific function: GTP-dependent nucleic acid-binding protein which may act as a translation factor [H]

COG id: COG0012

COG function: function code J; Predicted GTPase, probable translation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI58761500, Length=367, Percent_Identity=44.6866485013624, Blast_Score=305, Evalue=4e-83,
Organism=Homo sapiens, GI58761502, Length=232, Percent_Identity=37.9310344827586, Blast_Score=156, Evalue=2e-38,
Organism=Escherichia coli, GI1787454, Length=363, Percent_Identity=77.4104683195592, Blast_Score=590, Evalue=1e-170,
Organism=Escherichia coli, GI1789574, Length=127, Percent_Identity=29.9212598425197, Blast_Score=63, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17509631, Length=371, Percent_Identity=41.7789757412399, Blast_Score=293, Evalue=1e-79,
Organism=Saccharomyces cerevisiae, GI6319499, Length=368, Percent_Identity=40.2173913043478, Blast_Score=257, Evalue=2e-69,
Organism=Saccharomyces cerevisiae, GI6321773, Length=380, Percent_Identity=33.1578947368421, Blast_Score=196, Evalue=6e-51,
Organism=Saccharomyces cerevisiae, GI6321649, Length=140, Percent_Identity=40.7142857142857, Blast_Score=81, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24640873, Length=366, Percent_Identity=42.896174863388, Blast_Score=300, Evalue=1e-81,
Organism=Drosophila melanogaster, GI24640877, Length=366, Percent_Identity=42.896174863388, Blast_Score=300, Evalue=1e-81,
Organism=Drosophila melanogaster, GI24640875, Length=366, Percent_Identity=42.896174863388, Blast_Score=300, Evalue=1e-81,
Organism=Drosophila melanogaster, GI24640879, Length=328, Percent_Identity=40.5487804878049, Blast_Score=244, Evalue=8e-65,
Organism=Drosophila melanogaster, GI24585318, Length=197, Percent_Identity=27.9187817258883, Blast_Score=69, Evalue=7e-12,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR004396
- InterPro:   IPR013029
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR012676
- InterPro:   IPR023192 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF06071 YchF-GTPase_C [H]

EC number: NA

Molecular weight: Translated: 39282; Mature: 39151

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFKCGIVGLPNVGKSTLFNALTKAGIEASNFPFCTIEPNTGVVPVPDTRLDALAAIVKP
CCEEECEEECCCCCHHHHHHHHHHCCCCCCCCCEEEECCCCCEEECCCCHHHHHHHHHCC
ERVLATTMEFVDIAGLVAGASKGEGLGNKFLANIRETDAIGHVVRCFEDDNIVHVANKVD
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCC
PASDIEVINTELALADLDSCERAITRQAKRAKGGDTEAKFEVAVLEKMRPVLDEGKMLRS
CCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHH
MELTKEELAAIGYLNFLTLKPTMYIANVAEDGFENNPHLDTVKALAAEENAVVVAVCAAI
HHHHHHHHHHHHHHHEEEECCCEEEEEHHHCCCCCCCCHHHHHHHHCCCCCEEEEHHHHH
ESELAEMDAEDREEFMADLGLEEPGLDRVIRAGYDLLSLQTYFTAGVKEVRAWTVRIGAS
HHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHEEEEEEECCC
APQAAGVIHTDFERGFIRAQVMAYDDFITYKGEAGAKEAGKLRVEGKTYTVKDGDVMHFL
CCCCCCEEEECCCCCCEEEEEEEEECEEEECCCCCCCCCCEEEECCCEEEECCCCEEEEE
FNV
ECC
>Mature Secondary Structure 
GFKCGIVGLPNVGKSTLFNALTKAGIEASNFPFCTIEPNTGVVPVPDTRLDALAAIVKP
CEEECEEECCCCCHHHHHHHHHHCCCCCCCCCEEEECCCCCEEECCCCHHHHHHHHHCC
ERVLATTMEFVDIAGLVAGASKGEGLGNKFLANIRETDAIGHVVRCFEDDNIVHVANKVD
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCC
PASDIEVINTELALADLDSCERAITRQAKRAKGGDTEAKFEVAVLEKMRPVLDEGKMLRS
CCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHH
MELTKEELAAIGYLNFLTLKPTMYIANVAEDGFENNPHLDTVKALAAEENAVVVAVCAAI
HHHHHHHHHHHHHHHEEEECCCEEEEEHHHCCCCCCCCHHHHHHHHCCCCCEEEEHHHHH
ESELAEMDAEDREEFMADLGLEEPGLDRVIRAGYDLLSLQTYFTAGVKEVRAWTVRIGAS
HHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHEEEEEEECCC
APQAAGVIHTDFERGFIRAQVMAYDDFITYKGEAGAKEAGKLRVEGKTYTVKDGDVMHFL
CCCCCCEEEECCCCCCEEEEEEEEECEEEECCCCCCCCCCEEEECCCEEEECCCCEEEEE
FNV
ECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]