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The map label for this gene is proB [H]

Identifier: 157962903

GI number: 157962903

Start: 3784284

End: 3785402

Strand: Reverse

Name: proB [H]

Synonym: Spea_3085

Alternate gene names: 157962903

Gene position: 3785402-3784284 (Counterclockwise)

Preceding gene: 157962904

Following gene: 157962902

Centisome position: 73.15

GC content: 47.99

Gene sequence:

>1119_bases
ATGAACTTAAGTGAGATTGGCTACCGCCGCGTAGTGGTTAAATTGGGAACAAGTGTTCTGACATCTGGCAGTTTAAAGCT
GGATAAGGCGCACATGGTTGAATTAGCAAGACAGATGGCCTGCTTGATGAAAGCGGGGGTCGAAGTCGTATTGTGTACTT
CTGGTGCGATAGCCGCAGGTAAAGAGCATTTAGGCTACCCCAAATTACCCGATACCATAGCGAGTAAGCAATTACTTGCA
GCTGTCGGTCAAAGCCAGCTAATCCTTGCCTGGTCTCAGTTATTTAGTATCTATGGTTTGCATGTGGGTCAGCTGCTGCT
AACCCGTGCCGACTTACATGATAGAGAGCGTTACTTAAATGCTCGGGACTCACTCAATGCCTTGCTTAATAATGGCATTA
TCCCAATCATTAACGAGAATGACGCGGTAGCAACGGCTGAAATTAAAGTAGGCGACAATGATAATTTGTCTGCTCGCGCA
GCGCTGTTATGCGATGCAGATTTATTGATTTTATTGACAGATCAGAAAGGCTTATTCGATGCCGATCCGCGTAAGAACCC
TGATGCCAAGTTAATCACCGAAGTACAGAATATCGATGATAGCCTGCGTATGTTGGCGGGCGGTGCCGTTTCTGGTTTAG
GAACGGGCGGCATGGCGACTAAGTTAGAGGCGGCTGATATTGCGCGTCGCGCGGGTGTTGAGGTGGTTATTGCCTCTGGG
CACTATAAAGATGTGATTCAAAATGTAGTGTGTAAAAAGCCTGTCGGCACTCACTTTACAGCGCTTGAACATCCGCTTGA
GAGCCGCAAGCAGTGGATCTTAGCAGGGCCAAAGGCTCGTGGTCAGCTGGTTATCGATGCGGGGGCAATTGGCGCGGTGA
CAGAGAAGGGCAGAAGCTTGCTTTCTAAAGGGATCACTGAAGTTAAAGGCCTATTTCAGCGTGGTGATACCCTTGAACTG
ATTGATACCAAGGGTAAGGTATACGCTAAAGGCATGAGCCGTTATAGCTCTGCTGATGTGACTAAACTTGCTGGTAAGCA
CTCAGATAGCATCGAAGAGGTGCTCGGGTATGATTATGGTGATGCAGTAGTTCACCGCAACGACATGGTGGTGTTATGA

Upstream 100 bases:

>100_bases
TAAATCCGCCTTTTTTTTTAGATTCTTTGTTGGTGCTTACTACCAATTCAAACCTAGATTGGGTATAAGGATTTATTCAA
TGTAATTTTGTTAGCAAATA

Downstream 100 bases:

>100_bases
ATAACGACGAATATTTAACGGCTTTAGGACAGAATGCTAAGCAGGCGAGTTATGCTTTGGCCACGTTATCGGGTCAGCAA
AAATCTGCGCTACTTCGCTG

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MNLSEIGYRRVVVKLGTSVLTSGSLKLDKAHMVELARQMACLMKAGVEVVLCTSGAIAAGKEHLGYPKLPDTIASKQLLA
AVGQSQLILAWSQLFSIYGLHVGQLLLTRADLHDRERYLNARDSLNALLNNGIIPIINENDAVATAEIKVGDNDNLSARA
ALLCDADLLILLTDQKGLFDADPRKNPDAKLITEVQNIDDSLRMLAGGAVSGLGTGGMATKLEAADIARRAGVEVVIASG
HYKDVIQNVVCKKPVGTHFTALEHPLESRKQWILAGPKARGQLVIDAGAIGAVTEKGRSLLSKGITEVKGLFQRGDTLEL
IDTKGKVYAKGMSRYSSADVTKLAGKHSDSIEEVLGYDYGDAVVHRNDMVVL

Sequences:

>Translated_372_residues
MNLSEIGYRRVVVKLGTSVLTSGSLKLDKAHMVELARQMACLMKAGVEVVLCTSGAIAAGKEHLGYPKLPDTIASKQLLA
AVGQSQLILAWSQLFSIYGLHVGQLLLTRADLHDRERYLNARDSLNALLNNGIIPIINENDAVATAEIKVGDNDNLSARA
ALLCDADLLILLTDQKGLFDADPRKNPDAKLITEVQNIDDSLRMLAGGAVSGLGTGGMATKLEAADIARRAGVEVVIASG
HYKDVIQNVVCKKPVGTHFTALEHPLESRKQWILAGPKARGQLVIDAGAIGAVTEKGRSLLSKGITEVKGLFQRGDTLEL
IDTKGKVYAKGMSRYSSADVTKLAGKHSDSIEEVLGYDYGDAVVHRNDMVVL
>Mature_372_residues
MNLSEIGYRRVVVKLGTSVLTSGSLKLDKAHMVELARQMACLMKAGVEVVLCTSGAIAAGKEHLGYPKLPDTIASKQLLA
AVGQSQLILAWSQLFSIYGLHVGQLLLTRADLHDRERYLNARDSLNALLNNGIIPIINENDAVATAEIKVGDNDNLSARA
ALLCDADLLILLTDQKGLFDADPRKNPDAKLITEVQNIDDSLRMLAGGAVSGLGTGGMATKLEAADIARRAGVEVVIASG
HYKDVIQNVVCKKPVGTHFTALEHPLESRKQWILAGPKARGQLVIDAGAIGAVTEKGRSLLSKGITEVKGLFQRGDTLEL
IDTKGKVYAKGMSRYSSADVTKLAGKHSDSIEEVLGYDYGDAVVHRNDMVVL

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI62912457, Length=294, Percent_Identity=35.7142857142857, Blast_Score=138, Evalue=8e-33,
Organism=Homo sapiens, GI21361368, Length=296, Percent_Identity=35.472972972973, Blast_Score=137, Evalue=2e-32,
Organism=Escherichia coli, GI1786437, Length=360, Percent_Identity=61.9444444444444, Blast_Score=447, Evalue=1e-127,
Organism=Caenorhabditis elegans, GI17569967, Length=267, Percent_Identity=34.0823970037453, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17569969, Length=267, Percent_Identity=34.0823970037453, Blast_Score=130, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6320506, Length=415, Percent_Identity=37.1084337349398, Blast_Score=226, Evalue=5e-60,
Organism=Saccharomyces cerevisiae, GI6321822, Length=411, Percent_Identity=33.8199513381995, Blast_Score=211, Evalue=1e-55,
Organism=Drosophila melanogaster, GI21357643, Length=290, Percent_Identity=34.1379310344828, Blast_Score=130, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 39798; Mature: 39798

Theoretical pI: Translated: 7.63; Mature: 7.63

Prosite motif: PS50890 PUA ; PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLSEIGYRRVVVKLGTSVLTSGSLKLDKAHMVELARQMACLMKAGVEVVLCTSGAIAAG
CCCHHHHHHHHHHHHCHHHHCCCCCEECHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCC
KEHLGYPKLPDTIASKQLLAAVGQSQLILAWSQLFSIYGLHVGQLLLTRADLHDRERYLN
HHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHC
ARDSLNALLNNGIIPIINENDAVATAEIKVGDNDNLSARAALLCDADLLILLTDQKGLFD
HHHHHHHHHHCCEEEEECCCCCEEEEEEEECCCCCCCCEEEEEECCCEEEEEECCCCCCC
ADPRKNPDAKLITEVQNIDDSLRMLAGGAVSGLGTGGMATKLEAADIARRAGVEVVIASG
CCCCCCCCHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCHHEHHHHHHHHHCCCEEEEECC
HYKDVIQNVVCKKPVGTHFTALEHPLESRKQWILAGPKARGQLVIDAGAIGAVTEKGRSL
CHHHHHHHHHHCCCCCCCHHHHHCCHHHCCCEEEECCCCCCEEEEECCCCHHHHHHHHHH
LSKGITEVKGLFQRGDTLELIDTKGKVYAKGMSRYSSADVTKLAGKHSDSIEEVLGYDYG
HHHHHHHHHHHHHCCCEEEEEECCCCEEEHHHHHCCCCHHHHHHCCCCCCHHHHHCCCCC
DAVVHRNDMVVL
CCEEECCCEEEC
>Mature Secondary Structure
MNLSEIGYRRVVVKLGTSVLTSGSLKLDKAHMVELARQMACLMKAGVEVVLCTSGAIAAG
CCCHHHHHHHHHHHHCHHHHCCCCCEECHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCC
KEHLGYPKLPDTIASKQLLAAVGQSQLILAWSQLFSIYGLHVGQLLLTRADLHDRERYLN
HHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHC
ARDSLNALLNNGIIPIINENDAVATAEIKVGDNDNLSARAALLCDADLLILLTDQKGLFD
HHHHHHHHHHCCEEEEECCCCCEEEEEEEECCCCCCCCEEEEEECCCEEEEEECCCCCCC
ADPRKNPDAKLITEVQNIDDSLRMLAGGAVSGLGTGGMATKLEAADIARRAGVEVVIASG
CCCCCCCCHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCHHEHHHHHHHHHCCCEEEEECC
HYKDVIQNVVCKKPVGTHFTALEHPLESRKQWILAGPKARGQLVIDAGAIGAVTEKGRSL
CHHHHHHHHHHCCCCCCCHHHHHCCHHHCCCEEEECCCCCCEEEEECCCCHHHHHHHHHH
LSKGITEVKGLFQRGDTLELIDTKGKVYAKGMSRYSSADVTKLAGKHSDSIEEVLGYDYG
HHHHHHHHHHHHHCCCEEEEEECCCCEEEHHHHHCCCCHHHHHHCCCCCCHHHHHCCCCC
DAVVHRNDMVVL
CCEEECCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA