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The map label for this gene is phsA [H]

Identifier: 157962857

GI number: 157962857

Start: 3726403

End: 3728679

Strand: Reverse

Name: phsA [H]

Synonym: Spea_3039

Alternate gene names: 157962857

Gene position: 3728679-3726403 (Counterclockwise)

Preceding gene: 157962858

Following gene: 157962856

Centisome position: 72.06

GC content: 48.79

Gene sequence:

>2277_bases
ATGAAACTAGATCGGCGAACCTTTATCAAAGGGGCCGGAGCGGGTACAGCGACTTGCGCATTAGCGACGCTGCCAGGCTC
CTTAGCTGCCTTTGGTGATTCACAATTGCAAGGTAGTGCAGAGTCTGTAGCCAGTATTTGTGAGATGTGTTCAACTCGCT
GCCCGATATCGGCACGTGTCGTTAACGGCAAGAACGTATCGATTCTGGGTAATAAAAACGCCAAATCTTTTGGCGGTGCA
GTTTGTGCTCGCGGTGGTGCGGGGCATAGCCAGCTTTATGATAAACAGCGCATTGTTAAACCATTAAAGCGTGTTGGTGA
GCGTGGTGAAGGTAACTGGCAAGAGATTGAGTGGGATGAAGCCTATGAGCTAATTGCGCAGAACCTCAATAAGATCAAAG
CCGAACATGGCGCCGAGACTGTTGCTTTCTCATCTAAGTCTGGCTCATTATCCGGGCATCTTTTCCACTTAGCCAAAGCC
TTTGGCAGTCCAAATACCTTTACCCATGCATCGACCTGCCCTGGCAGCTATGTTATCGCGGCAAAGGCGATGTTTGGCGG
TAAAATTAAGCGTGATTTAAGCAATTCTAAGTACATCATTAACTTCGGTCATAACCTCTACGAAGGCATCAACATGTCTG
AGACAAGGGGCATGATGAATGCACAAATGGAGAAAGGCGCTAAGCTGGTGGTATTTGAACCACGCTTCTCGATTGTGGCG
GACAAGGCTGATGAGTGGTACGCGATTAAACCGGGCACCGACGTTGCTGTGGCATTGGCAATTTGTCATGTCTTGATTGA
AGATGGCCTATACGATAAAGCTTTCGTTGCCCAGTATGTATCGGGTTTTGATGAGTTCGCCAAAGAAGTGAAGGCTTACA
CGCCAGAGTGGGCCGAGTCTATTAGTGATGTGCCTGCCGAAGACATTCGCCGTATTACTCATGAATACGCAGCGGCTGCC
CCACATGCGATGGTAGATTTTGGTCACCGTTCATCGTTTACTCCTGAAGAGTTTGAAATGCGCCGTGCGCTTTACGCAGC
CAACGTATTGATTGGTAATATTGAGCGTAAAGGTGGCTTGTACTTTGGTAAGAAGGCAGCGAGTTACAACAAGTTTGCCG
GTGAGGCGGTCGCACCAACCTTAGCTAAACCGGGTGTCGATGGCATGCCAAAAATTGATGCTAAGCGTATCGATATGGTC
GATGAGCAGTATGCCCTAACTTGGTCTTCGGGTGGTATCTATCAAACCATTCTTGATGCAACGCTGGAAGCGACGCCGTA
CCAACTACGCGCTTGGGTAATGAGCCGTACGAACCCTATGCAGACCATGACTGACAGAGCACGCATCGTTAAGACGATGC
AGAAGCTCGATTTTATAGTGGCTTGCGATATCTACATCAGTGAGAGCGCGGCTTACGCCGATGTCTTCCTACCTGAGTCT
ACTTACCTCGAGCGTGATGAAGAGATTGCCGATAACTCGGGTAATCACCCTGCTTACTATGTGCGTCAACGTGTCGTAGA
AACGATTGGTGACACTAAACCGAGCTGGTTAATCTGGAAAGAACTTGCTGACAGGCTTGGTCAAGGTCAATTCTTCCCTT
GGGAGAACATGGAAACCTTACAGCTGTTCCAAGTACAGCGTGACTTTGACTTATTGAGAAAAATTAAAGATGACGGTTAC
GTCAGCTTTGGTAAGCCGCTAATGCTACGTGAGCGTGGCATGGTGAGAGACTTTGTTAATACCTACCCTAATGCAAAAGC
GGTCGATGAAGATGGCACTTATGCAAGTCAGTTAAGCTTTAAGACACCAACAGGCAAAATTGAATTGAGCTCTGCCAAGG
TCGAGAAGATGGCGGCGGGCCGCGGCGTTATTCGCTACCGTGAAGTGACGATGAAAAAAGCCGATGAGCTGTTTTTCATT
CAAGGAAAAGTCGCGGTGCACACTAACGCGGGTACTCAAAACGTACCAATGCTTGCGAATCTAATGTCAGACAATGCCGT
TTGGATCCATCCCATTACCGCGGGTTTACTAAAGATCGCCAGTGGTGACAAGATTAGGATTTACAACGATACCGGCAGCG
AAGAGGGACATGCTCTCGTGACGCCAGGTATTCGCCCTGACACCGTATTTGCCTATATGGGCTGCGGCTCAAAGAACAAG
GAGCTTGCTCGTGCCACCGGTAAAGGCGTGCATTGTGGCAACCTACTTCCCCACGTGACTTCTCCGGTGACAGGCATGAA
TCTGCATACCACCGGCGTTAAGATTGCCAAGATTTAA

Upstream 100 bases:

>100_bases
ACAATGATGAAAACCTTAGGAAGAAGTTGATTGTGCTAAGCAGTTAACTTGGGCTTTATCTAAAGCGCTCCTAAGGACAA
CTGTCATTGGGAGATTTATC

Downstream 100 bases:

>100_bases
TCGGAGTTAATTATGACTAAGCGTTATGTTCTCGTGCACGATGAAAACAAGTGCATTGGCTGTCAGGCTTGTAACGTGGC
TTGCCGTAGTGAAAACAATG

Product: molybdopterin oxidoreductase

Products: Reduced form of N-Oxide compounds [C]

Alternate protein names: NA

Number of amino acids: Translated: 758; Mature: 758

Protein sequence:

>758_residues
MKLDRRTFIKGAGAGTATCALATLPGSLAAFGDSQLQGSAESVASICEMCSTRCPISARVVNGKNVSILGNKNAKSFGGA
VCARGGAGHSQLYDKQRIVKPLKRVGERGEGNWQEIEWDEAYELIAQNLNKIKAEHGAETVAFSSKSGSLSGHLFHLAKA
FGSPNTFTHASTCPGSYVIAAKAMFGGKIKRDLSNSKYIINFGHNLYEGINMSETRGMMNAQMEKGAKLVVFEPRFSIVA
DKADEWYAIKPGTDVAVALAICHVLIEDGLYDKAFVAQYVSGFDEFAKEVKAYTPEWAESISDVPAEDIRRITHEYAAAA
PHAMVDFGHRSSFTPEEFEMRRALYAANVLIGNIERKGGLYFGKKAASYNKFAGEAVAPTLAKPGVDGMPKIDAKRIDMV
DEQYALTWSSGGIYQTILDATLEATPYQLRAWVMSRTNPMQTMTDRARIVKTMQKLDFIVACDIYISESAAYADVFLPES
TYLERDEEIADNSGNHPAYYVRQRVVETIGDTKPSWLIWKELADRLGQGQFFPWENMETLQLFQVQRDFDLLRKIKDDGY
VSFGKPLMLRERGMVRDFVNTYPNAKAVDEDGTYASQLSFKTPTGKIELSSAKVEKMAAGRGVIRYREVTMKKADELFFI
QGKVAVHTNAGTQNVPMLANLMSDNAVWIHPITAGLLKIASGDKIRIYNDTGSEEGHALVTPGIRPDTVFAYMGCGSKNK
ELARATGKGVHCGNLLPHVTSPVTGMNLHTTGVKIAKI

Sequences:

>Translated_758_residues
MKLDRRTFIKGAGAGTATCALATLPGSLAAFGDSQLQGSAESVASICEMCSTRCPISARVVNGKNVSILGNKNAKSFGGA
VCARGGAGHSQLYDKQRIVKPLKRVGERGEGNWQEIEWDEAYELIAQNLNKIKAEHGAETVAFSSKSGSLSGHLFHLAKA
FGSPNTFTHASTCPGSYVIAAKAMFGGKIKRDLSNSKYIINFGHNLYEGINMSETRGMMNAQMEKGAKLVVFEPRFSIVA
DKADEWYAIKPGTDVAVALAICHVLIEDGLYDKAFVAQYVSGFDEFAKEVKAYTPEWAESISDVPAEDIRRITHEYAAAA
PHAMVDFGHRSSFTPEEFEMRRALYAANVLIGNIERKGGLYFGKKAASYNKFAGEAVAPTLAKPGVDGMPKIDAKRIDMV
DEQYALTWSSGGIYQTILDATLEATPYQLRAWVMSRTNPMQTMTDRARIVKTMQKLDFIVACDIYISESAAYADVFLPES
TYLERDEEIADNSGNHPAYYVRQRVVETIGDTKPSWLIWKELADRLGQGQFFPWENMETLQLFQVQRDFDLLRKIKDDGY
VSFGKPLMLRERGMVRDFVNTYPNAKAVDEDGTYASQLSFKTPTGKIELSSAKVEKMAAGRGVIRYREVTMKKADELFFI
QGKVAVHTNAGTQNVPMLANLMSDNAVWIHPITAGLLKIASGDKIRIYNDTGSEEGHALVTPGIRPDTVFAYMGCGSKNK
ELARATGKGVHCGNLLPHVTSPVTGMNLHTTGVKIAKI
>Mature_758_residues
MKLDRRTFIKGAGAGTATCALATLPGSLAAFGDSQLQGSAESVASICEMCSTRCPISARVVNGKNVSILGNKNAKSFGGA
VCARGGAGHSQLYDKQRIVKPLKRVGERGEGNWQEIEWDEAYELIAQNLNKIKAEHGAETVAFSSKSGSLSGHLFHLAKA
FGSPNTFTHASTCPGSYVIAAKAMFGGKIKRDLSNSKYIINFGHNLYEGINMSETRGMMNAQMEKGAKLVVFEPRFSIVA
DKADEWYAIKPGTDVAVALAICHVLIEDGLYDKAFVAQYVSGFDEFAKEVKAYTPEWAESISDVPAEDIRRITHEYAAAA
PHAMVDFGHRSSFTPEEFEMRRALYAANVLIGNIERKGGLYFGKKAASYNKFAGEAVAPTLAKPGVDGMPKIDAKRIDMV
DEQYALTWSSGGIYQTILDATLEATPYQLRAWVMSRTNPMQTMTDRARIVKTMQKLDFIVACDIYISESAAYADVFLPES
TYLERDEEIADNSGNHPAYYVRQRVVETIGDTKPSWLIWKELADRLGQGQFFPWENMETLQLFQVQRDFDLLRKIKDDGY
VSFGKPLMLRERGMVRDFVNTYPNAKAVDEDGTYASQLSFKTPTGKIELSSAKVEKMAAGRGVIRYREVTMKKADELFFI
QGKVAVHTNAGTQNVPMLANLMSDNAVWIHPITAGLLKIASGDKIRIYNDTGSEEGHALVTPGIRPDTVFAYMGCGSKNK
ELARATGKGVHCGNLLPHVTSPVTGMNLHTTGVKIAKI

Specific function: Oxidoreductase which produces hydrogen sulfide from thiosulfate (Potential) [H]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasm Face Of The Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081797, Length=847, Percent_Identity=26.6824085005903, Blast_Score=196, Evalue=5e-51,
Organism=Escherichia coli, GI1787870, Length=843, Percent_Identity=26.4531435349941, Blast_Score=191, Evalue=2e-49,
Organism=Escherichia coli, GI171474008, Length=842, Percent_Identity=24.8218527315914, Blast_Score=186, Evalue=4e-48,
Organism=Escherichia coli, GI3868721, Length=589, Percent_Identity=26.1460101867572, Blast_Score=161, Evalue=2e-40,
Organism=Escherichia coli, GI145693196, Length=668, Percent_Identity=22.9041916167665, Blast_Score=112, Evalue=6e-26,
Organism=Escherichia coli, GI87081994, Length=803, Percent_Identity=23.0386052303861, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI1787231, Length=715, Percent_Identity=21.2587412587413, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI3868720, Length=311, Percent_Identity=25.7234726688103, Blast_Score=82, Evalue=2e-16,
Organism=Escherichia coli, GI1788534, Length=550, Percent_Identity=20.7272727272727, Blast_Score=72, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655
- InterPro:   IPR006311 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: 1.8.99.- [C]

Molecular weight: Translated: 83173; Mature: 83173

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: PS00464 RIBOSOMAL_L22 ; PS00551 MOLYBDOPTERIN_PROK_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLDRRTFIKGAGAGTATCALATLPGSLAAFGDSQLQGSAESVASICEMCSTRCPISARV
CCCCCCHHEECCCCCHHEEEEECCCCCHHHCCCHHCCCCHHHHHHHHHHHHCCCCCEEEE
VNGKNVSILGNKNAKSFGGAVCARGGAGHSQLYDKQRIVKPLKRVGERGEGNWQEIEWDE
ECCCEEEEEECCCCCHHCCHHEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
AYELIAQNLNKIKAEHGAETVAFSSKSGSLSGHLFHLAKAFGSPNTFTHASTCPGSYVIA
HHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEE
AKAMFGGKIKRDLSNSKYIINFGHNLYEGINMSETRGMMNAQMEKGAKLVVFEPRFSIVA
EHHHHCCHHHHHCCCCEEEEECCCHHHHCCCCHHHHHHHHHHHHCCCEEEEECCCCEEEE
DKADEWYAIKPGTDVAVALAICHVLIEDGLYDKAFVAQYVSGFDEFAKEVKAYTPEWAES
CCCCCEEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
ISDVPAEDIRRITHEYAAAAPHAMVDFGHRSSFTPEEFEMRRALYAANVLIGNIERKGGL
HHCCCHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHEECCCCCCCE
YFGKKAASYNKFAGEAVAPTLAKPGVDGMPKIDAKRIDMVDEQYALTWSSGGIYQTILDA
EECCCCCCCHHHCCCHHCCHHCCCCCCCCCCCCHHHHCCCCCCEEEEECCCCHHHHHHHH
TLEATPYQLRAWVMSRTNPMQTMTDRARIVKTMQKLDFIVACDIYISESAAYADVFLPES
HHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCEEEEECCCC
TYLERDEEIADNSGNHPAYYVRQRVVETIGDTKPSWLIWKELADRLGQGQFFPWENMETL
HHHCCCHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHH
QLFQVQRDFDLLRKIKDDGYVSFGKPLMLRERGMVRDFVNTYPNAKAVDEDGTYASQLSF
HHHHHHHHHHHHHHHCCCCCEECCCCHHHHHCCHHHHHHHHCCCCCCCCCCCCCHHEEEE
KTPTGKIELSSAKVEKMAAGRGVIRYREVTMKKADELFFIQGKVAVHTNAGTQNVPMLAN
CCCCCEEEECHHHHHHHHCCCCCEEHHHHHHHCCCCEEEEEEEEEEEECCCCCCCCHHHH
LMSDNAVWIHPITAGLLKIASGDKIRIYNDTGSEEGHALVTPGIRPDTVFAYMGCGSKNK
HHCCCCEEEEEHHHHHHHHCCCCEEEEECCCCCCCCCEEECCCCCCCCEEEEECCCCCCC
ELARATGKGVHCGNLLPHVTSPVTGMNLHTTGVKIAKI
HHHHHCCCCCCCCCCCCHHCCCCCCCEEEECCEEEEEC
>Mature Secondary Structure
MKLDRRTFIKGAGAGTATCALATLPGSLAAFGDSQLQGSAESVASICEMCSTRCPISARV
CCCCCCHHEECCCCCHHEEEEECCCCCHHHCCCHHCCCCHHHHHHHHHHHHCCCCCEEEE
VNGKNVSILGNKNAKSFGGAVCARGGAGHSQLYDKQRIVKPLKRVGERGEGNWQEIEWDE
ECCCEEEEEECCCCCHHCCHHEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
AYELIAQNLNKIKAEHGAETVAFSSKSGSLSGHLFHLAKAFGSPNTFTHASTCPGSYVIA
HHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEE
AKAMFGGKIKRDLSNSKYIINFGHNLYEGINMSETRGMMNAQMEKGAKLVVFEPRFSIVA
EHHHHCCHHHHHCCCCEEEEECCCHHHHCCCCHHHHHHHHHHHHCCCEEEEECCCCEEEE
DKADEWYAIKPGTDVAVALAICHVLIEDGLYDKAFVAQYVSGFDEFAKEVKAYTPEWAES
CCCCCEEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
ISDVPAEDIRRITHEYAAAAPHAMVDFGHRSSFTPEEFEMRRALYAANVLIGNIERKGGL
HHCCCHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHEECCCCCCCE
YFGKKAASYNKFAGEAVAPTLAKPGVDGMPKIDAKRIDMVDEQYALTWSSGGIYQTILDA
EECCCCCCCHHHCCCHHCCHHCCCCCCCCCCCCHHHHCCCCCCEEEEECCCCHHHHHHHH
TLEATPYQLRAWVMSRTNPMQTMTDRARIVKTMQKLDFIVACDIYISESAAYADVFLPES
HHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCEEEEECCCC
TYLERDEEIADNSGNHPAYYVRQRVVETIGDTKPSWLIWKELADRLGQGQFFPWENMETL
HHHCCCHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHH
QLFQVQRDFDLLRKIKDDGYVSFGKPLMLRERGMVRDFVNTYPNAKAVDEDGTYASQLSF
HHHHHHHHHHHHHHHCCCCCEECCCCHHHHHCCHHHHHHHHCCCCCCCCCCCCCHHEEEE
KTPTGKIELSSAKVEKMAAGRGVIRYREVTMKKADELFFIQGKVAVHTNAGTQNVPMLAN
CCCCCEEEECHHHHHHHHCCCCCEEHHHHHHHCCCCEEEEEEEEEEEECCCCCCCCHHHH
LMSDNAVWIHPITAGLLKIASGDKIRIYNDTGSEEGHALVTPGIRPDTVFAYMGCGSKNK
HHCCCCEEEEEHHHHHHHHCCCCEEEEECCCCCCCCCEEECCCCCCCCEEEEECCCCCCC
ELARATGKGVHCGNLLPHVTSPVTGMNLHTTGVKIAKI
HHHHHCCCCCCCCCCCCHHCCCCCCCEEEECCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: May Bind 4Fe-4S Cluster. [C]

Metal ions: Fe; Mo [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-Oxide; Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

Specific reaction: Reduces Various N-Oxide And Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7751291; 11677609; 7737516 [H]