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The map label for this gene is psrB [H]

Identifier: 157962856

GI number: 157962856

Start: 3725818

End: 3726390

Strand: Reverse

Name: psrB [H]

Synonym: Spea_3038

Alternate gene names: 157962856

Gene position: 3726390-3725818 (Counterclockwise)

Preceding gene: 157962857

Following gene: 157962855

Centisome position: 72.01

GC content: 48.87

Gene sequence:

>573_bases
ATGACTAAGCGTTATGTTCTCGTGCACGATGAAAACAAGTGCATTGGCTGTCAGGCTTGTAACGTGGCTTGCCGTAGTGA
AAACAATGTGCCTGAGTCGGTGACTCGTATTCAGGTGAGAATTGAGGGGCCTTACGGCAACTTCCCGCACCTACATTTTA
AGTATAACCGCGTCTCTTGTGAGCAGTGTGAAAACGCGCCTTGCGTTAAGGTGTGTCCAACCGGCGCAGCCTATGTTAAT
GATGACGGCATTGTATCGATTAACGAGAAGAAGTGTGTCGGCTGTCTATATTGTGTCGCGGCTTGTCCTTACAAGGTGCG
TTTCATTAACCCTGAAACCCGAGTGCCTGATAAGTGCGATTTCTGTAAAGAAACACGCTTGGCTCGAGGTGAAGAGCCTG
CTTGTGTCACGGTTTGCCCAACCCAAGCACTGGTGTTTGGTGATGCCAATGACATGACATCGGAAGTGCGCAAAGTGCTA
GACACAAAAAATCACTACCAACAGAAGGTAAACCTTGGCACTGAGCCACGTGTCTACCGCATTCCTAGCCGTAAAGGAGG
GATCAAAGCATGA

Upstream 100 bases:

>100_bases
GCATTGTGGCAACCTACTTCCCCACGTGACTTCTCCGGTGACAGGCATGAATCTGCATACCACCGGCGTTAAGATTGCCA
AGATTTAATCGGAGTTAATT

Downstream 100 bases:

>100_bases
ACAATATCTGGGGCTCTATGGAGCAATACGATCCGGTAGTTTGGAACTGGATCATCGCGGTGTATTTGTTTATGGCGGGC
TTATCTGCTGGCTCAATGCT

Product: 4Fe-4S ferredoxin iron-sulfur-binding domain-containing protein

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: Sulfur reductase chain B [H]

Number of amino acids: Translated: 190; Mature: 189

Protein sequence:

>190_residues
MTKRYVLVHDENKCIGCQACNVACRSENNVPESVTRIQVRIEGPYGNFPHLHFKYNRVSCEQCENAPCVKVCPTGAAYVN
DDGIVSINEKKCVGCLYCVAACPYKVRFINPETRVPDKCDFCKETRLARGEEPACVTVCPTQALVFGDANDMTSEVRKVL
DTKNHYQQKVNLGTEPRVYRIPSRKGGIKA

Sequences:

>Translated_190_residues
MTKRYVLVHDENKCIGCQACNVACRSENNVPESVTRIQVRIEGPYGNFPHLHFKYNRVSCEQCENAPCVKVCPTGAAYVN
DDGIVSINEKKCVGCLYCVAACPYKVRFINPETRVPDKCDFCKETRLARGEEPACVTVCPTQALVFGDANDMTSEVRKVL
DTKNHYQQKVNLGTEPRVYRIPSRKGGIKA
>Mature_189_residues
TKRYVLVHDENKCIGCQACNVACRSENNVPESVTRIQVRIEGPYGNFPHLHFKYNRVSCEQCENAPCVKVCPTGAAYVND
DGIVSINEKKCVGCLYCVAACPYKVRFINPETRVPDKCDFCKETRLARGEEPACVTVCPTQALVFGDANDMTSEVRKVLD
TKNHYQQKVNLGTEPRVYRIPSRKGGIKA

Specific function: Component of the phosphorylative electron transport system with polysulfide as the terminal acceptor [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI2367345, Length=186, Percent_Identity=51.0752688172043, Blast_Score=206, Evalue=6e-55,
Organism=Escherichia coli, GI226510944, Length=177, Percent_Identity=42.9378531073446, Blast_Score=172, Evalue=1e-44,
Organism=Escherichia coli, GI1787872, Length=159, Percent_Identity=37.7358490566038, Blast_Score=102, Evalue=1e-23,
Organism=Escherichia coli, GI1787122, Length=159, Percent_Identity=37.7358490566038, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1787749, Length=159, Percent_Identity=33.3333333333333, Blast_Score=91, Evalue=3e-20,
Organism=Escherichia coli, GI1790326, Length=162, Percent_Identity=32.7160493827161, Blast_Score=90, Evalue=7e-20,
Organism=Escherichia coli, GI1789067, Length=169, Percent_Identity=35.5029585798817, Blast_Score=84, Evalue=6e-18,
Organism=Escherichia coli, GI87082180, Length=187, Percent_Identity=34.2245989304813, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI87082179, Length=145, Percent_Identity=36.551724137931, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI1789370, Length=206, Percent_Identity=30.0970873786408, Blast_Score=81, Evalue=5e-17,
Organism=Escherichia coli, GI1787963, Length=162, Percent_Identity=28.3950617283951, Blast_Score=77, Evalue=8e-16,
Organism=Escherichia coli, GI1788811, Length=148, Percent_Identity=33.7837837837838, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI2367245, Length=153, Percent_Identity=33.9869281045752, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI1787478, Length=137, Percent_Identity=32.1167883211679, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787740, Length=96, Percent_Identity=38.5416666666667, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1789079, Length=185, Percent_Identity=29.7297297297297, Blast_Score=60, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 21255; Mature: 21123

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

8.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
9.5 %Cys+Met (Translated Protein)
8.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
9.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKRYVLVHDENKCIGCQACNVACRSENNVPESVTRIQVRIEGPYGNFPHLHFKYNRVSC
CCCEEEEEECCCCEEEEEECCEEECCCCCCCCCEEEEEEEEECCCCCCCEEEEEECCCCH
EQCENAPCVKVCPTGAAYVNDDGIVSINEKKCVGCLYCVAACPYKVRFINPETRVPDKCD
HHHCCCCEEEECCCCCEEECCCCEEEECCCCHHHHHHHHHHCCEEEEEECCCCCCCCCCH
FCKETRLARGEEPACVTVCPTQALVFGDANDMTSEVRKVLDTKNHYQQKVNLGTEPRVYR
HHHHHHHCCCCCCCEEEECCCEEEEEECCCHHHHHHHHHHHCHHHHHHHHCCCCCCEEEE
IPSRKGGIKA
CCCCCCCCCC
>Mature Secondary Structure 
TKRYVLVHDENKCIGCQACNVACRSENNVPESVTRIQVRIEGPYGNFPHLHFKYNRVSC
CCEEEEEECCCCEEEEEECCEEECCCCCCCCCEEEEEEEEECCCCCCCEEEEEECCCCH
EQCENAPCVKVCPTGAAYVNDDGIVSINEKKCVGCLYCVAACPYKVRFINPETRVPDKCD
HHHCCCCEEEECCCCCEEECCCCEEEECCCCHHHHHHHHHHCCEEEEEECCCCCCCCCCH
FCKETRLARGEEPACVTVCPTQALVFGDANDMTSEVRKVLDTKNHYQQKVNLGTEPRVYR
HHHHHHHCCCCCCCEEEECCCEEEEEECCCHHHHHHHHHHHCHHHHHHHHCCCCCCEEEE
IPSRKGGIKA
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1597189; 14500908 [H]