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The map label for this gene is nrfA [H]

Identifier: 157962632

GI number: 157962632

Start: 3433543

End: 3434943

Strand: Reverse

Name: nrfA [H]

Synonym: Spea_2812

Alternate gene names: 157962632

Gene position: 3434943-3433543 (Counterclockwise)

Preceding gene: 157962634

Following gene: 157962628

Centisome position: 66.38

GC content: 46.9

Gene sequence:

>1401_bases
ATGGATGGATACCCTATGAGAGCATACCAACTCCCTTTGATTGCTGGAGTTTGCATAGGTTTGTTTAGTAGCGCAGCAAT
TGCAAAAACGAACCCTGCAGATAATGCCCAGTACGAAAAACGCTTTCCTAAGCAATACCAAAGCTGGCAAAAAACAGCTG
AGAGTAAAGAGGTGGATGACGCATTAGCAGGTGACCCTAACTTGGTCATTCTGTGGGCGGGTTATGGTTTCTCTAAGGAT
TATAATGCCCCTAGAGGGCACCAATATGCTATTACCGATGTGAGAACCACGTTACGAACCGGCGCCCCAATGAAAGAAGA
TGAAGGCCCTATGCCGATGGCCTGCTGGAGTTGTAAGAGCCCAGATGTTCCGAGAGTTATCGAGGGACAAGGCGAAGGGG
AGTATTTCAGTGGTAAGTGGGCTAAAGGTGGCCCTGAAATCGTCAATGTGATTGGCTGTGCAGATTGTCATAAACCTGGG
ACATCAAAGTTAAGAGTTGCACGACCCTTTGCCGAACGCGCCTTTGAAGCGATAGGTACACCGTTTGATAAGGCTGAAAA
GAGCGAAAAGCAAAATATGGTGTGCGGACAGTGTCATGTAGAGTATTACTTTGAGAAAACGGCTGAGCACCCAAACTGGG
TTAAGTTCCCTTGGGATAACGGCACCACGGTAGAGAATATGGAAGCGTATTATGACAAGTTGAAGTTCAAAGATTGGACT
CATCAACTGTCAAAAGCACCAATGTTAAAAGCTCAGCATCCTGGCTATGAAACCACAGCGCTAGGTATGCACGGAGATAT
GGGCGTATCTTGTACCGATTGTCATATGCCGCGAGTCACCAATGCTGAGGGGAAGGAATACACCGACCATAATGTGGGTA
GCCCATTTGATAAGTTTGAATTTACCTGCGCAAATTGTCATGAGCAGACCAAAGAGCAATTGCAGTCTTTAGTTAAAGCG
AATAAAGATAAGGTCAATGCGCTTAAGCTTAAAGCTGAGGCAGAACTCGTTAAAGCTCACTTTGAAGCGGCTGCAGCATG
GAAAGCAGGAGCGACAGAGCAGCAAATGGCGGCGGCATTAACCGATATTCGTCACGCACAGTGGCGCTGGGACTTTGCGA
TTGCGTCCCACGGTATTGCGGCACATGCCCCAGAAGAAGGCTTACGCATTTTAGAAACCTCACTGACTAAAAGCGCTGAA
GCTCGGACTAAATTGGCGAAAGTCTTGGATACTCAAGGCGTTAAAGCTGCGGTCACCATTCCTGATATTTCAACTAAAGC
AAAAGCACAAGCGGTCTTAGGCATGGATATGGATAAGATGAATGCAGATAAAGCCATCTTTATCGAGAAAGTCGTGCCTA
AATGGGAGGCTGACTATAAGGCAGCCAATGGCGGTGAATAG

Upstream 100 bases:

>100_bases
CTCTATCTACAGCTTTCTTTTGTTTGATAACTAATTTTATGCGTTTTTTGACAATGCATTAGCAGCGACTACATTTTATA
TATCACAATACCCTTATGTA

Downstream 100 bases:

>100_bases
GTTTTAATTGCTGCGCTTCAATATAATCCGGGAAGTGAACTTCCCGGATTTTTTGTTTTCTGCTTAGAGTCTGTAATGGG
ATTAATTAGGTCTATATCGC

Product: nitrite reductase

Products: NA

Alternate protein names: Ammonia-forming cytochrome c nitrite reductase; Cytochrome c nitrite reductase [H]

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MDGYPMRAYQLPLIAGVCIGLFSSAAIAKTNPADNAQYEKRFPKQYQSWQKTAESKEVDDALAGDPNLVILWAGYGFSKD
YNAPRGHQYAITDVRTTLRTGAPMKEDEGPMPMACWSCKSPDVPRVIEGQGEGEYFSGKWAKGGPEIVNVIGCADCHKPG
TSKLRVARPFAERAFEAIGTPFDKAEKSEKQNMVCGQCHVEYYFEKTAEHPNWVKFPWDNGTTVENMEAYYDKLKFKDWT
HQLSKAPMLKAQHPGYETTALGMHGDMGVSCTDCHMPRVTNAEGKEYTDHNVGSPFDKFEFTCANCHEQTKEQLQSLVKA
NKDKVNALKLKAEAELVKAHFEAAAAWKAGATEQQMAAALTDIRHAQWRWDFAIASHGIAAHAPEEGLRILETSLTKSAE
ARTKLAKVLDTQGVKAAVTIPDISTKAKAQAVLGMDMDKMNADKAIFIEKVVPKWEADYKAANGGE

Sequences:

>Translated_466_residues
MDGYPMRAYQLPLIAGVCIGLFSSAAIAKTNPADNAQYEKRFPKQYQSWQKTAESKEVDDALAGDPNLVILWAGYGFSKD
YNAPRGHQYAITDVRTTLRTGAPMKEDEGPMPMACWSCKSPDVPRVIEGQGEGEYFSGKWAKGGPEIVNVIGCADCHKPG
TSKLRVARPFAERAFEAIGTPFDKAEKSEKQNMVCGQCHVEYYFEKTAEHPNWVKFPWDNGTTVENMEAYYDKLKFKDWT
HQLSKAPMLKAQHPGYETTALGMHGDMGVSCTDCHMPRVTNAEGKEYTDHNVGSPFDKFEFTCANCHEQTKEQLQSLVKA
NKDKVNALKLKAEAELVKAHFEAAAAWKAGATEQQMAAALTDIRHAQWRWDFAIASHGIAAHAPEEGLRILETSLTKSAE
ARTKLAKVLDTQGVKAAVTIPDISTKAKAQAVLGMDMDKMNADKAIFIEKVVPKWEADYKAANGGE
>Mature_466_residues
MDGYPMRAYQLPLIAGVCIGLFSSAAIAKTNPADNAQYEKRFPKQYQSWQKTAESKEVDDALAGDPNLVILWAGYGFSKD
YNAPRGHQYAITDVRTTLRTGAPMKEDEGPMPMACWSCKSPDVPRVIEGQGEGEYFSGKWAKGGPEIVNVIGCADCHKPG
TSKLRVARPFAERAFEAIGTPFDKAEKSEKQNMVCGQCHVEYYFEKTAEHPNWVKFPWDNGTTVENMEAYYDKLKFKDWT
HQLSKAPMLKAQHPGYETTALGMHGDMGVSCTDCHMPRVTNAEGKEYTDHNVGSPFDKFEFTCANCHEQTKEQLQSLVKA
NKDKVNALKLKAEAELVKAHFEAAAAWKAGATEQQMAAALTDIRHAQWRWDFAIASHGIAAHAPEEGLRILETSLTKSAE
ARTKLAKVLDTQGVKAAVTIPDISTKAKAQAVLGMDMDKMNADKAIFIEKVVPKWEADYKAANGGE

Specific function: Plays a role in nitrite reduction [H]

COG id: COG3303

COG function: function code P; Formate-dependent nitrite reductase, periplasmic cytochrome c552 subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cytochrome c-552 family [H]

Homologues:

Organism=Escherichia coli, GI1790506, Length=455, Percent_Identity=58.2417582417582, Blast_Score=553, Evalue=1e-158,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003321
- InterPro:   IPR017570
- InterPro:   IPR011031 [H]

Pfam domain/function: PF02335 Cytochrom_C552 [H]

EC number: =1.7.2.2 [H]

Molecular weight: Translated: 51512; Mature: 51512

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: PS51008 MULTIHEME_CYTC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDGYPMRAYQLPLIAGVCIGLFSSAAIAKTNPADNAQYEKRFPKQYQSWQKTAESKEVDD
CCCCCCCHHHHHHHHHHHHHHHHCCCEECCCCCCCCHHHHHCHHHHHHHHHHHHHHHHHH
ALAGDPNLVILWAGYGFSKDYNAPRGHQYAITDVRTTLRTGAPMKEDEGPMPMACWSCKS
HHCCCCCEEEEEECCCCCCCCCCCCCCEEEHHHHHHHHHCCCCCCCCCCCCCEEEECCCC
PDVPRVIEGQGEGEYFSGKWAKGGPEIVNVIGCADCHKPGTSKLRVARPFAERAFEAIGT
CCCCCEECCCCCCCEECCCCCCCCHHHEEEEECCCCCCCCCCCEEEECHHHHHHHHHHCC
PFDKAEKSEKQNMVCGQCHVEYYFEKTAEHPNWVKFPWDNGTTVENMEAYYDKLKFKDWT
CHHHHHHHHHHCCEEHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHCCHHHH
HQLSKAPMLKAQHPGYETTALGMHGDMGVSCTDCHMPRVTNAEGKEYTDHNVGSPFDKFE
HHHHCCCCCCCCCCCCCEEEECCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCHHHE
FTCANCHEQTKEQLQSLVKANKDKVNALKLKAEAELVKAHFEAAAAWKAGATEQQMAAAL
EEHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
TDIRHAQWRWDFAIASHGIAAHAPEEGLRILETSLTKSAEARTKLAKVLDTQGVKAAVTI
HHHHHHHEEEEHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
PDISTKAKAQAVLGMDMDKMNADKAIFIEKVVPKWEADYKAANGGE
CCCCCHHHHHHHHCCCHHHCCCCCEEEHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MDGYPMRAYQLPLIAGVCIGLFSSAAIAKTNPADNAQYEKRFPKQYQSWQKTAESKEVDD
CCCCCCCHHHHHHHHHHHHHHHHCCCEECCCCCCCCHHHHHCHHHHHHHHHHHHHHHHHH
ALAGDPNLVILWAGYGFSKDYNAPRGHQYAITDVRTTLRTGAPMKEDEGPMPMACWSCKS
HHCCCCCEEEEEECCCCCCCCCCCCCCEEEHHHHHHHHHCCCCCCCCCCCCCEEEECCCC
PDVPRVIEGQGEGEYFSGKWAKGGPEIVNVIGCADCHKPGTSKLRVARPFAERAFEAIGT
CCCCCEECCCCCCCEECCCCCCCCHHHEEEEECCCCCCCCCCCEEEECHHHHHHHHHHCC
PFDKAEKSEKQNMVCGQCHVEYYFEKTAEHPNWVKFPWDNGTTVENMEAYYDKLKFKDWT
CHHHHHHHHHHCCEEHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHCCHHHH
HQLSKAPMLKAQHPGYETTALGMHGDMGVSCTDCHMPRVTNAEGKEYTDHNVGSPFDKFE
HHHHCCCCCCCCCCCCCEEEECCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCHHHE
FTCANCHEQTKEQLQSLVKANKDKVNALKLKAEAELVKAHFEAAAAWKAGATEQQMAAAL
EEHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
TDIRHAQWRWDFAIASHGIAAHAPEEGLRILETSLTKSAEARTKLAKVLDTQGVKAAVTI
HHHHHHHEEEEHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
PDISTKAKAQAVLGMDMDKMNADKAIFIEKVVPKWEADYKAANGGE
CCCCCHHHHHHHHCCCHHHCCCCCEEEHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA