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The map label for this gene is mexA [H]

Identifier: 157962628

GI number: 157962628

Start: 3429470

End: 3430606

Strand: Reverse

Name: mexA [H]

Synonym: Spea_2808

Alternate gene names: 157962628

Gene position: 3430606-3429470 (Counterclockwise)

Preceding gene: 157962632

Following gene: 157962627

Centisome position: 66.3

GC content: 45.03

Gene sequence:

>1137_bases
ATGGCTAGTGAATTAAAAAAAATTACCCGTTTAGGGCTGTTTGCAGTGGTCTGTTTCTGGACGACAGCCTGTGAGAAAAG
TGGCACTGACGAGGCGTTTAATTATCCAAACACCGCCAATATTGCCACCGTAACCGCCGAGTTAACTCAAGTCACCTTAT
TTGATGAGTTACAAGGGCGAGTGCGCCCATTACGTAGCGCTGAAATTCGCCCTCAGGTTGAAGGGATCATCACCCAAAGA
TTATTTAAACAAGGAGATACGCTTGAGCAAGGCGATGCACTGTTTCAAATTGACCAAGACATTTTCATTATCGATGTTGA
GATAAAGCAAGCAGCGCTTGAGCAATCTATGGCTCACTTAGCGTTAATACAAAGCCAAGTTGATCGCTTTACTCAATTAG
ATCGTACTAAGGCGGTAAGTAAGCAGGCTTTTGAAGAGGCCAGTTTTAACTTACAAATTGCAGCCGCCACTGTGCAGCAA
AATCGAGCTCAACTTAAGCATAGTGAGTTACAACTTGAGTACGCGAAAGTAACAGCTCCTATCTCAGGGATTATCGGTGA
GGCATTAGTGACTGAGGGAAGTTTAGTGAGCCGTACCGAGCCACAGCCATTAGCCATCATTCAGCAGATTGATAAGGTCT
ATATCGATGTTCGTCAACCCGCATCGAAACTTGGGGTATTGCGACGTTTATTAGCCAATGAACAGAAAAACAGTGAGCAA
GGGGTCAAGGTGATTGTGCAGTCGTCTGCAGAGAGTGCAGATGATATTGAAGGGCAAATTTTATTCTCAGGGATCAGCGT
TGATGAAAATACCGGCGATCTGGTTATTCGAATTATTGCTGATAACGCTAAGCAACAGCTGCTACCAGGCATGTATGTAC
GAACTCAAATTCCCCGTCAGAAGTTACAAGCAATCACAGTTCCGGAGCACGCGGTTCGCCGTAACAGTGCGGGCTCGCCG
TTTGTCTACATTGTTGTTAACGATAAGTTTGAGCAAAGACCAGTACAACTAGAAGGGCTGCAACAGCGACGATATGTCAT
TTCGAAAGGTATTAATCCAAACGATATTGTGGTTGTGACTGGCCAAGATAATCTGCGCGAAGGGGCTGAGTTAGTCCTGT
CAGATTGGCAACAGTAA

Upstream 100 bases:

>100_bases
TTGCTCATTTAATCTGTCGCAATCAATTTTAATGCCTAGGTTTTGTCTTCATTGAGATACAACATAGGCTGTGCCTGTCA
GGTATGAGTGAAGATAATAT

Downstream 100 bases:

>100_bases
TGTATCAACGGCTAGTGTAAGAGGAAGTTTATTGTGTCGCATTTTTTTATTTCTCGACCTGTTTTTGCATGGGTTATTGC
TATTTTAATTAGCATCGCAG

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 378; Mature: 377

Protein sequence:

>378_residues
MASELKKITRLGLFAVVCFWTTACEKSGTDEAFNYPNTANIATVTAELTQVTLFDELQGRVRPLRSAEIRPQVEGIITQR
LFKQGDTLEQGDALFQIDQDIFIIDVEIKQAALEQSMAHLALIQSQVDRFTQLDRTKAVSKQAFEEASFNLQIAAATVQQ
NRAQLKHSELQLEYAKVTAPISGIIGEALVTEGSLVSRTEPQPLAIIQQIDKVYIDVRQPASKLGVLRRLLANEQKNSEQ
GVKVIVQSSAESADDIEGQILFSGISVDENTGDLVIRIIADNAKQQLLPGMYVRTQIPRQKLQAITVPEHAVRRNSAGSP
FVYIVVNDKFEQRPVQLEGLQQRRYVISKGINPNDIVVVTGQDNLREGAELVLSDWQQ

Sequences:

>Translated_378_residues
MASELKKITRLGLFAVVCFWTTACEKSGTDEAFNYPNTANIATVTAELTQVTLFDELQGRVRPLRSAEIRPQVEGIITQR
LFKQGDTLEQGDALFQIDQDIFIIDVEIKQAALEQSMAHLALIQSQVDRFTQLDRTKAVSKQAFEEASFNLQIAAATVQQ
NRAQLKHSELQLEYAKVTAPISGIIGEALVTEGSLVSRTEPQPLAIIQQIDKVYIDVRQPASKLGVLRRLLANEQKNSEQ
GVKVIVQSSAESADDIEGQILFSGISVDENTGDLVIRIIADNAKQQLLPGMYVRTQIPRQKLQAITVPEHAVRRNSAGSP
FVYIVVNDKFEQRPVQLEGLQQRRYVISKGINPNDIVVVTGQDNLREGAELVLSDWQQ
>Mature_377_residues
ASELKKITRLGLFAVVCFWTTACEKSGTDEAFNYPNTANIATVTAELTQVTLFDELQGRVRPLRSAEIRPQVEGIITQRL
FKQGDTLEQGDALFQIDQDIFIIDVEIKQAALEQSMAHLALIQSQVDRFTQLDRTKAVSKQAFEEASFNLQIAAATVQQN
RAQLKHSELQLEYAKVTAPISGIIGEALVTEGSLVSRTEPQPLAIIQQIDKVYIDVRQPASKLGVLRRLLANEQKNSEQG
VKVIVQSSAESADDIEGQILFSGISVDENTGDLVIRIIADNAKQQLLPGMYVRTQIPRQKLQAITVPEHAVRRNSAGSPF
VYIVVNDKFEQRPVQLEGLQQRRYVISKGINPNDIVVVTGQDNLREGAELVLSDWQQ

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor. Note=The membrane anchor is not necessary for antibiotic efflux as the protein is functional when targeted to the periplasm by a foreign signal peptide [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786668, Length=355, Percent_Identity=33.8028169014084, Blast_Score=178, Evalue=4e-46,
Organism=Escherichia coli, GI1789665, Length=322, Percent_Identity=33.8509316770186, Blast_Score=167, Evalue=8e-43,
Organism=Escherichia coli, GI1789929, Length=351, Percent_Identity=31.3390313390313, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI87082036, Length=317, Percent_Identity=24.6056782334385, Blast_Score=91, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 42004; Mature: 41873

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASELKKITRLGLFAVVCFWTTACEKSGTDEAFNYPNTANIATVTAELTQVTLFDELQGR
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHH
VRPLRSAEIRPQVEGIITQRLFKQGDTLEQGDALFQIDQDIFIIDVEIKQAALEQSMAHL
HCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCEEEEEEHHHHHHHHHHHHHH
ALIQSQVDRFTQLDRTKAVSKQAFEEASFNLQIAAATVQQNRAQLKHSELQLEYAKVTAP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHEEEHHHHHCC
ISGIIGEALVTEGSLVSRTEPQPLAIIQQIDKVYIDVRQPASKLGVLRRLLANEQKNSEQ
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHEECCCCHHHHHHHHHHHHCCCCCCCC
GVKVIVQSSAESADDIEGQILFSGISVDENTGDLVIRIIADNAKQQLLPGMYVRTQIPRQ
CCEEEEECCCCCCCCCCCEEEEEECCCCCCCCCEEEEEEECCCHHHHCCCHHHHHHCCHH
KLQAITVPEHAVRRNSAGSPFVYIVVNDKFEQRPVQLEGLQQRRYVISKGINPNDIVVVT
HHHEECCCHHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEE
GQDNLREGAELVLSDWQQ
CCCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
ASELKKITRLGLFAVVCFWTTACEKSGTDEAFNYPNTANIATVTAELTQVTLFDELQGR
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHH
VRPLRSAEIRPQVEGIITQRLFKQGDTLEQGDALFQIDQDIFIIDVEIKQAALEQSMAHL
HCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCEEEEEEHHHHHHHHHHHHHH
ALIQSQVDRFTQLDRTKAVSKQAFEEASFNLQIAAATVQQNRAQLKHSELQLEYAKVTAP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHEEEHHHHHCC
ISGIIGEALVTEGSLVSRTEPQPLAIIQQIDKVYIDVRQPASKLGVLRRLLANEQKNSEQ
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHEECCCCHHHHHHHHHHHHCCCCCCCC
GVKVIVQSSAESADDIEGQILFSGISVDENTGDLVIRIIADNAKQQLLPGMYVRTQIPRQ
CCEEEEECCCCCCCCCCCEEEEEECCCCCCCCCEEEEEEECCCHHHHCCCHHHHHHCCHH
KLQAITVPEHAVRRNSAGSPFVYIVVNDKFEQRPVQLEGLQQRRYVISKGINPNDIVVVT
HHHEECCCHHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEE
GQDNLREGAELVLSDWQQ
CCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7968531; 10984043; 9989496; 8226684; 8540696; 9603892; 10671490 [H]