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The map label for this gene is leuO [H]

Identifier: 157962569

GI number: 157962569

Start: 3358517

End: 3359419

Strand: Reverse

Name: leuO [H]

Synonym: Spea_2748

Alternate gene names: 157962569

Gene position: 3359419-3358517 (Counterclockwise)

Preceding gene: 157962571

Following gene: 157962568

Centisome position: 64.92

GC content: 42.75

Gene sequence:

>903_bases
ATGATATTCGATAAATTTGATCTCAATTTGCTTAAAGTATTTTCTAAGGTGATGGAAACCGGTAGCTACGTGAAGACGAG
TGAAGCGCTGGGTATCTCTCCGCCTGCGGTGAGTATTGCGATGAAACGCTTACAAAAATCGCTAGAGAAAGAGCTCTTTG
TTCGAGGAGGAGGGCAGATCCAACCCACTGCAGCTGCGAAGGCTTTATACCAAAATGTTCGGTTTGAACTTTTGTCTATA
GAGACGGTGATTAGCGCTTTTGGACAGTTTGATCCTCATGTGTCGAAGGCACACTTTAGCTTAATGGGCCCTGAAGAATA
TAACGCTAAGTTACTGTCTGCATTTGCAGGCGAGCAAAACAATGGCTTGACCTATAGTTTGATGCAACAGGCGAGCACGG
ATGAAGAAGCTATTGCGAGTTTACGAACCCGAATGACAGACTTGGTTATCGATAGTGTCGTGCTAGCAGATAGTAGCATC
GAAAGTGAGCTACTGTTTGAAGATAGGATAGTCTTGATTGCAGCAAAAAGTAACTCGACAATCGATGAAAACTTGACCTT
CGAGCAGTATCAAGAGTTGCCGCAATCCGTGCTGAGTTTACGTCGAAGCGGTAAGTTGGCTTTGGAGATGTTTCTCGAAG
ATAAGCTTGAAGTTCGAAGAAATATTAGCCATGAGGCTAGCTCTATTATGGCAAATATATTGATTGTTAGCCAAACAATG
CTGTTCTGTCATGTGCCACTCAGGCTTGCTATACACTACCAAGAGCAGCTCTCTCTTAAAATCATAGAACCACCAATTAG
CCTCAAGCCTGTGCCGGTTATTATGCAGTGGCATAAATCAAATTCGATAGATCCTAGTCATATTTGGTTGCGAAATAGAA
TTAAACAGATATTGCTGCCGTAA

Upstream 100 bases:

>100_bases
CAGATTAAATTCATAGCACCAATTTACACTGGTAGTCAGATAAACTAAATTGACTAGAGTTTATCTAAGAGATAAGCACT
GTTTATCTTGCGGGGCTGTA

Downstream 100 bases:

>100_bases
ACAGGATATGACTTTGCTAAAAACACTGCCGATATAATTTGTTGCCTAGATCTAGTATGAGTTTCGGCTACTAGGGATAG
AGTTTTTTTAGGCGCGGTAA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MIFDKFDLNLLKVFSKVMETGSYVKTSEALGISPPAVSIAMKRLQKSLEKELFVRGGGQIQPTAAAKALYQNVRFELLSI
ETVISAFGQFDPHVSKAHFSLMGPEEYNAKLLSAFAGEQNNGLTYSLMQQASTDEEAIASLRTRMTDLVIDSVVLADSSI
ESELLFEDRIVLIAAKSNSTIDENLTFEQYQELPQSVLSLRRSGKLALEMFLEDKLEVRRNISHEASSIMANILIVSQTM
LFCHVPLRLAIHYQEQLSLKIIEPPISLKPVPVIMQWHKSNSIDPSHIWLRNRIKQILLP

Sequences:

>Translated_300_residues
MIFDKFDLNLLKVFSKVMETGSYVKTSEALGISPPAVSIAMKRLQKSLEKELFVRGGGQIQPTAAAKALYQNVRFELLSI
ETVISAFGQFDPHVSKAHFSLMGPEEYNAKLLSAFAGEQNNGLTYSLMQQASTDEEAIASLRTRMTDLVIDSVVLADSSI
ESELLFEDRIVLIAAKSNSTIDENLTFEQYQELPQSVLSLRRSGKLALEMFLEDKLEVRRNISHEASSIMANILIVSQTM
LFCHVPLRLAIHYQEQLSLKIIEPPISLKPVPVIMQWHKSNSIDPSHIWLRNRIKQILLP
>Mature_300_residues
MIFDKFDLNLLKVFSKVMETGSYVKTSEALGISPPAVSIAMKRLQKSLEKELFVRGGGQIQPTAAAKALYQNVRFELLSI
ETVISAFGQFDPHVSKAHFSLMGPEEYNAKLLSAFAGEQNNGLTYSLMQQASTDEEAIASLRTRMTDLVIDSVVLADSSI
ESELLFEDRIVLIAAKSNSTIDENLTFEQYQELPQSVLSLRRSGKLALEMFLEDKLEVRRNISHEASSIMANILIVSQTM
LFCHVPLRLAIHYQEQLSLKIIEPPISLKPVPVIMQWHKSNSIDPSHIWLRNRIKQILLP

Specific function: Unknown

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081684, Length=293, Percent_Identity=29.0102389078498, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1786820, Length=286, Percent_Identity=28.3216783216783, Blast_Score=86, Evalue=4e-18,

Paralogues:

None

Copy number: 825 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33731; Mature: 33731

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFDKFDLNLLKVFSKVMETGSYVKTSEALGISPPAVSIAMKRLQKSLEKELFVRGGGQI
CCCCCCCHHHHHHHHHHHHCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
QPTAAAKALYQNVRFELLSIETVISAFGQFDPHVSKAHFSLMGPEEYNAKLLSAFAGEQN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHEEEECCHHHHHHHHHHHCCCCC
NGLTYSLMQQASTDEEAIASLRTRMTDLVIDSVVLADSSIESELLFEDRIVLIAAKSNST
CCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHEECCCEEEEEECCCCC
IDENLTFEQYQELPQSVLSLRRSGKLALEMFLEDKLEVRRNISHEASSIMANILIVSQTM
CCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
LFCHVPLRLAIHYQEQLSLKIIEPPISLKPVPVIMQWHKSNSIDPSHIWLRNRIKQILLP
HHHHHHHHHEEEEHHCCEEEEECCCCCCCCCHHEEEECCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIFDKFDLNLLKVFSKVMETGSYVKTSEALGISPPAVSIAMKRLQKSLEKELFVRGGGQI
CCCCCCCHHHHHHHHHHHHCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
QPTAAAKALYQNVRFELLSIETVISAFGQFDPHVSKAHFSLMGPEEYNAKLLSAFAGEQN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHEEEECCHHHHHHHHHHHCCCCC
NGLTYSLMQQASTDEEAIASLRTRMTDLVIDSVVLADSSIESELLFEDRIVLIAAKSNST
CCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHEECCCEEEEEECCCCC
IDENLTFEQYQELPQSVLSLRRSGKLALEMFLEDKLEVRRNISHEASSIMANILIVSQTM
CCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
LFCHVPLRLAIHYQEQLSLKIIEPPISLKPVPVIMQWHKSNSIDPSHIWLRNRIKQILLP
HHHHHHHHHEEEEHHCCEEEEECCCCCCCCCHHEEEECCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3413113; 1630901; 9278503; 3519576 [H]