Click here to switch to the map view.

The map label for this gene is lcfB [H]

Identifier: 157962555

GI number: 157962555

Start: 3340420

End: 3342141

Strand: Reverse

Name: lcfB [H]

Synonym: Spea_2734

Alternate gene names: 157962555

Gene position: 3342141-3340420 (Counterclockwise)

Preceding gene: 157962556

Following gene: 157962554

Centisome position: 64.59

GC content: 49.13

Gene sequence:

>1722_bases
ATGGTAAGTCAGTTGATTCAAAACAATGACGGCGCAAATTTGTGCCGTCATTTAGTTAGTGCTGCCCAGACGATTCCACA
CTCGCTGGCGGTCGCCGTGCAGCAAGCCAATGGCAAATCAGTCGGTGGCTTACATTATCAAGAGATGGACTTTCAAACTC
TTAACGCCAAAAGTGACGAGCTTGCCAGTGCGCTAGTGAACCACGGCTTAACGCCTGGTATGAAGGCGGTATTGATGGTC
ACGCCTAGCATTGATTTCTTCTGCCTAACCTTCGCGCTGTTTAAGGCTGGTATCATTCCAATCTTGGTTGACCCAGGCAT
GGGGATAAAAAACCTTAAACAGTGTTTTATTGAGGCTAAACCCGACGCTTTTATCGGGATTCCCAAGGCGCACATTGCCA
GACGTCTTTTTGGTTGGGGCAAGTCTAGCGTTAAACATCTGATCAATGTGGGGGGCAGCGGTTTTGCTCAGGCATTGACT
GGCGCGGCAAGCTTAGAAAGCCTATTGAAAGCTAATCCTTCGGCAACAACTAAGCCTTTTAAGATGCAGTGGCTCGATGA
TGATGCCATGGCGGCAATCTTGTTTACCAGCGGCAGTACTGGTACGCCAAAGGGTGTGGTCTATTCCCATAAGATATTTG
AGGCGCAAATTACCGCGCTTAAACATGACTACGCCATCGAGCCTGGTGAGCGCGACCTTGCAACCTTCCCGCTGTTTTCA
CTGTTTGGCCCAGCTCTAGGCATGGCATCTATCGTGCCGGATATGGATGCCAGTAAACCCATTACCGCCAATCCTGATTT
TATCTTTGCCGCCATCGATAAATATCAGTGCAGTAATATGTTCGTCAATCCGGCGCTTATTGAGCGTCTTGGTGCGGCAG
GTGAAGCCAATCAACACAAGCTGAGTAGCATCAGGCGGGTGATCTCGGCCGGAGCGCCTGCGACCATCTCATCGATTAAA
CGCTTTAGTCAGATGCTTAATCATGGCGTAGAGGTGCTTAACTCCTACGGTGCGACCGAATCTCTGCCTATTAGCAAAAT
AGGAAGTCAGGCGCTGTTTGACACCACGGCTGCGACAGATAACGGCGGCGGAATTTGTGTCGGTAAGGCAGTCGATGGAG
TCGATATCGCCATCATTAAGATTGATGAACAACCTGTTGAGCATTGGGATGACTGTTTGCGCTTGCCTGTAGATTCAATC
GGTGAAATCGTCGTTAAAGGTCCTATGGTGAGCCGCAGCTATTATCAGCGAGACAGCGCAACCCAAGTGGCAAAAATTCA
AGATGGTGACAGTATTCGCCACCGTATGGGCGACTTAGGTTATCTCGATAATGACGGTTTGCTGTGGATGTGCGGCCGCA
AGGCTCATAGAGTCGACGCCGATGTTTTGCCGGCAACTAATAACCCAGCTAAACGTTATTACTCTATTCCTTGTGAGCGT
ATTTTTAATACTCATAGTGATGTAAAGCGTAGTGCTTTGGTGGGCGTTATGGTGGCGGGCAAAGTCACGCCACTTATCTG
TATCGAGCTTAAGCAGTCAGTTGCCTGTTCGTTGTCGGCTGTACTTTACGGTGAGCTAAGAGCGATTGCCGAGCAGCATC
TGCAGACTCAAGGTATCGAGCGCTTCCTCATTCATCCAGATTTTCCTGTCGATATCCGCCACAACGCGAAGATTTTCCGT
GAAAAGTTAGCGGTATGGGCACAAAAGCAGATCAAGGAGTAA

Upstream 100 bases:

>100_bases
ATATTTTGGAAGATGCCAGTGATGAAGTGATTGGCCATATTCAAGCCTTTATCGCAAAAACCGACAAACAAACTCCTACT
GAATCGGTAGCCTAAACTCC

Downstream 100 bases:

>100_bases
TGCATCTTGTCATTGGTTAACACGAGTCAACCACCAACGCAGACACCAAAGCTTGACGACTTCTTGCCCCAAGAGCAGGC
CGCGCTGCTTGCACTTAAGC

Product: peptide synthase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 573; Mature: 573

Protein sequence:

>573_residues
MVSQLIQNNDGANLCRHLVSAAQTIPHSLAVAVQQANGKSVGGLHYQEMDFQTLNAKSDELASALVNHGLTPGMKAVLMV
TPSIDFFCLTFALFKAGIIPILVDPGMGIKNLKQCFIEAKPDAFIGIPKAHIARRLFGWGKSSVKHLINVGGSGFAQALT
GAASLESLLKANPSATTKPFKMQWLDDDAMAAILFTSGSTGTPKGVVYSHKIFEAQITALKHDYAIEPGERDLATFPLFS
LFGPALGMASIVPDMDASKPITANPDFIFAAIDKYQCSNMFVNPALIERLGAAGEANQHKLSSIRRVISAGAPATISSIK
RFSQMLNHGVEVLNSYGATESLPISKIGSQALFDTTAATDNGGGICVGKAVDGVDIAIIKIDEQPVEHWDDCLRLPVDSI
GEIVVKGPMVSRSYYQRDSATQVAKIQDGDSIRHRMGDLGYLDNDGLLWMCGRKAHRVDADVLPATNNPAKRYYSIPCER
IFNTHSDVKRSALVGVMVAGKVTPLICIELKQSVACSLSAVLYGELRAIAEQHLQTQGIERFLIHPDFPVDIRHNAKIFR
EKLAVWAQKQIKE

Sequences:

>Translated_573_residues
MVSQLIQNNDGANLCRHLVSAAQTIPHSLAVAVQQANGKSVGGLHYQEMDFQTLNAKSDELASALVNHGLTPGMKAVLMV
TPSIDFFCLTFALFKAGIIPILVDPGMGIKNLKQCFIEAKPDAFIGIPKAHIARRLFGWGKSSVKHLINVGGSGFAQALT
GAASLESLLKANPSATTKPFKMQWLDDDAMAAILFTSGSTGTPKGVVYSHKIFEAQITALKHDYAIEPGERDLATFPLFS
LFGPALGMASIVPDMDASKPITANPDFIFAAIDKYQCSNMFVNPALIERLGAAGEANQHKLSSIRRVISAGAPATISSIK
RFSQMLNHGVEVLNSYGATESLPISKIGSQALFDTTAATDNGGGICVGKAVDGVDIAIIKIDEQPVEHWDDCLRLPVDSI
GEIVVKGPMVSRSYYQRDSATQVAKIQDGDSIRHRMGDLGYLDNDGLLWMCGRKAHRVDADVLPATNNPAKRYYSIPCER
IFNTHSDVKRSALVGVMVAGKVTPLICIELKQSVACSLSAVLYGELRAIAEQHLQTQGIERFLIHPDFPVDIRHNAKIFR
EKLAVWAQKQIKE
>Mature_573_residues
MVSQLIQNNDGANLCRHLVSAAQTIPHSLAVAVQQANGKSVGGLHYQEMDFQTLNAKSDELASALVNHGLTPGMKAVLMV
TPSIDFFCLTFALFKAGIIPILVDPGMGIKNLKQCFIEAKPDAFIGIPKAHIARRLFGWGKSSVKHLINVGGSGFAQALT
GAASLESLLKANPSATTKPFKMQWLDDDAMAAILFTSGSTGTPKGVVYSHKIFEAQITALKHDYAIEPGERDLATFPLFS
LFGPALGMASIVPDMDASKPITANPDFIFAAIDKYQCSNMFVNPALIERLGAAGEANQHKLSSIRRVISAGAPATISSIK
RFSQMLNHGVEVLNSYGATESLPISKIGSQALFDTTAATDNGGGICVGKAVDGVDIAIIKIDEQPVEHWDDCLRLPVDSI
GEIVVKGPMVSRSYYQRDSATQVAKIQDGDSIRHRMGDLGYLDNDGLLWMCGRKAHRVDADVLPATNNPAKRYYSIPCER
IFNTHSDVKRSALVGVMVAGKVTPLICIELKQSVACSLSAVLYGELRAIAEQHLQTQGIERFLIHPDFPVDIRHNAKIFR
EKLAVWAQKQIKE

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Escherichia coli, GI145693145, Length=304, Percent_Identity=26.6447368421053, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788107, Length=475, Percent_Identity=22.7368421052632, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1789201, Length=280, Percent_Identity=25.3571428571429, Blast_Score=70, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI71994694, Length=454, Percent_Identity=24.0088105726872, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71994703, Length=454, Percent_Identity=24.0088105726872, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71994690, Length=454, Percent_Identity=24.0088105726872, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17558820, Length=279, Percent_Identity=26.1648745519713, Blast_Score=79, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17559526, Length=313, Percent_Identity=24.6006389776358, Blast_Score=72, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17531443, Length=354, Percent_Identity=23.1638418079096, Blast_Score=69, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24648676, Length=482, Percent_Identity=24.2738589211618, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI21356947, Length=306, Percent_Identity=25.8169934640523, Blast_Score=76, Evalue=8e-14,
Organism=Drosophila melanogaster, GI19922652, Length=502, Percent_Identity=22.9083665338645, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24648260, Length=307, Percent_Identity=24.4299674267101, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI18859661, Length=277, Percent_Identity=24.5487364620939, Blast_Score=70, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24653035, Length=340, Percent_Identity=24.7058823529412, Blast_Score=69, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 61960; Mature: 61960

Theoretical pI: Translated: 7.79; Mature: 7.79

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSQLIQNNDGANLCRHLVSAAQTIPHSLAVAVQQANGKSVGGLHYQEMDFQTLNAKSDE
CCCHHHHCCCHHHHHHHHHHHHHHCCHHHHHHEECCCCCCCCCCEEECCCCHHCCCCHHH
LASALVNHGLTPGMKAVLMVTPSIDFFCLTFALFKAGIIPILVDPGMGIKNLKQCFIEAK
HHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHCC
PDAFIGIPKAHIARRLFGWGKSSVKHLINVGGSGFAQALTGAASLESLLKANPSATTKPF
CCCEEECCHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCE
KMQWLDDDAMAAILFTSGSTGTPKGVVYSHKIFEAQITALKHDYAIEPGERDLATFPLFS
EEEEECCCCEEEEEEECCCCCCCCCEEEEHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
LFGPALGMASIVPDMDASKPITANPDFIFAAIDKYQCSNMFVNPALIERLGAAGEANQHK
HHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECHHCCCCEEECHHHHHHHCCCCCCCHHH
LSSIRRVISAGAPATISSIKRFSQMLNHGVEVLNSYGATESLPISKIGSQALFDTTAATD
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCHHHHHCCCCCC
NGGGICVGKAVDGVDIAIIKIDEQPVEHWDDCLRLPVDSIGEIVVKGPMVSRSYYQRDSA
CCCCEEEECCCCCCEEEEEEECCCHHHHHHHHHHCCHHHHCCEEECCCCCCHHHHHCCCC
TQVAKIQDGDSIRHRMGDLGYLDNDGLLWMCGRKAHRVDADVLPATNNPAKRYYSIPCER
CCEEEECCCHHHHHHHCCCCEECCCCEEEEECCCCCCCCCCCCCCCCCHHHHEECCCHHH
IFNTHSDVKRSALVGVMVAGKVTPLICIELKQSVACSLSAVLYGELRAIAEQHLQTQGIE
HHCCHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RFLIHPDFPVDIRHNAKIFREKLAVWAQKQIKE
EEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVSQLIQNNDGANLCRHLVSAAQTIPHSLAVAVQQANGKSVGGLHYQEMDFQTLNAKSDE
CCCHHHHCCCHHHHHHHHHHHHHHCCHHHHHHEECCCCCCCCCCEEECCCCHHCCCCHHH
LASALVNHGLTPGMKAVLMVTPSIDFFCLTFALFKAGIIPILVDPGMGIKNLKQCFIEAK
HHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHCC
PDAFIGIPKAHIARRLFGWGKSSVKHLINVGGSGFAQALTGAASLESLLKANPSATTKPF
CCCEEECCHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCE
KMQWLDDDAMAAILFTSGSTGTPKGVVYSHKIFEAQITALKHDYAIEPGERDLATFPLFS
EEEEECCCCEEEEEEECCCCCCCCCEEEEHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
LFGPALGMASIVPDMDASKPITANPDFIFAAIDKYQCSNMFVNPALIERLGAAGEANQHK
HHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECHHCCCCEEECHHHHHHHCCCCCCCHHH
LSSIRRVISAGAPATISSIKRFSQMLNHGVEVLNSYGATESLPISKIGSQALFDTTAATD
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCHHHHHCCCCCC
NGGGICVGKAVDGVDIAIIKIDEQPVEHWDDCLRLPVDSIGEIVVKGPMVSRSYYQRDSA
CCCCEEEECCCCCCEEEEEEECCCHHHHHHHHHHCCHHHHCCEEECCCCCCHHHHHCCCC
TQVAKIQDGDSIRHRMGDLGYLDNDGLLWMCGRKAHRVDADVLPATNNPAKRYYSIPCER
CCEEEECCCHHHHHHHCCCCEECCCCEEEEECCCCCCCCCCCCCCCCCHHHHEECCCHHH
IFNTHSDVKRSALVGVMVAGKVTPLICIELKQSVACSLSAVLYGELRAIAEQHLQTQGIE
HHCCHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RFLIHPDFPVDIRHNAKIFREKLAVWAQKQIKE
EEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]