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The map label for this gene is ybjS [C]

Identifier: 157962554

GI number: 157962554

Start: 3339271

End: 3340413

Strand: Reverse

Name: ybjS [C]

Synonym: Spea_2733

Alternate gene names: 157962554

Gene position: 3340413-3339271 (Counterclockwise)

Preceding gene: 157962555

Following gene: 157962553

Centisome position: 64.55

GC content: 50.04

Gene sequence:

>1143_bases
TTGTCATTGGTTAACACGAGTCAACCACCAACGCAGACACCAAAGCTTGACGACTTCTTGCCCCAAGAGCAGGCCGCGCT
GCTTGCACTTAAGCAAGATGTGACTCGCGCCTTTGTAACCGGCGCAGGAGGCTTTTTAGGTAAAGCTATCTGTGAGCGAC
TACTCGCTGCTGGCATCGAGGTCATAGGCTTTGCTCGCGGTGACTACCCAGCATTGAGCGCCATGGGCGTTGAAATGGTT
AAAGGGGATATTGCCGATGAACGCGCGTTGCTTGGTGCTATGCAAGGCTGTGATCTGGTGTTTCATGTGGCATCGAAAGC
CGGGGTTTGGGGCAGCAAGAAGAGTTACTTCTCCCCCAATGTCGATGGAGCTCGTCACATCATTGAGGCCTGTAAGGCAT
GCAATATTGAGCGCCTAGTTTATACCAGTACACCGAGCGTCACCTTTGCCGGTGTGGATGAAGTGGGCAATGACGAGTCT
GCGCCATATGCAGAGCAGTTTCTTAATTATTACGGTGAGTCAAAGGCGATTGCCGAGCAGATGATGCTAGCGGCTAACGG
CGCTATGCTAGCTAATGGCAAACTGTTGGGCACCACGGCGTTAAGACCACATCTTATTTGGGGGCCAAATGATCCTCATC
TTGTGCCACGAGTGCTTGAGCGCGCCGAGGCGGGCAGGCTCAAGCTAGTAGGAAGTGAAGATAAGCTTGTCGATACCATC
TATGTGGGTAATGCCGCCTATGCTCATATCTTGGCGGCACTTACACTGAGTAATGGAGTGAGTAAAGGACAGGTTGAAGA
TAAGAATGCTAATCAGGCTGCTTGCGCGGGTAAGGCGTATTACTTGAGTAACGATGAACCGATTACGATGGCGACCATGC
TTAATAACATTCTTGCTTGTAAAAATTTGCCGCCAGTGACTAAGCGAGTACCGACTAATCTTGCCTACGCCGCCGGATGT
ATACTAGAGTCTATTTACGCTTTGCTGGGTAAAAAAGATGAGCCTATCATGACTCGCTTTGTCGCCAGACAGCTTTCCAC
TAGCCATTATTTCGATATTAGCGCGGCTAAGCGGGATCTAGGGTATCAGGCTTTAGTCAGTATTGACGAAGGGATGGAGC
AGCTTAAGGCGTCTTTACAATAA

Upstream 100 bases:

>100_bases
CATTCATCCAGATTTTCCTGTCGATATCCGCCACAACGCGAAGATTTTCCGTGAAAAGTTAGCGGTATGGGCACAAAAGC
AGATCAAGGAGTAATGCATC

Downstream 100 bases:

>100_bases
AGCTGACTAGGTTAATACTCACAACTCATGGACGTAAAAAACAGCAATATATTTGCTGTTTTTTTATGGGAAAAATAAGA
TTTGTTTATCAACTGACTGA

Product: 3-beta hydroxysteroid dehydrogenase/isomerase

Products: NA

Alternate protein names: Cholesterol dehydrogenase; 3-beta-hydroxy-Delta(5)-steroid dehydrogenase; 3-beta-HSD; 3BHSD; 3-beta hydroxysterol dehydrogenase; 3-beta-hydroxy-5-ene steroid dehydrogenase; Progesterone reductase; Steroid Delta-isomerase; Delta-5-3-ketosteroid isomerase [H]

Number of amino acids: Translated: 380; Mature: 379

Protein sequence:

>380_residues
MSLVNTSQPPTQTPKLDDFLPQEQAALLALKQDVTRAFVTGAGGFLGKAICERLLAAGIEVIGFARGDYPALSAMGVEMV
KGDIADERALLGAMQGCDLVFHVASKAGVWGSKKSYFSPNVDGARHIIEACKACNIERLVYTSTPSVTFAGVDEVGNDES
APYAEQFLNYYGESKAIAEQMMLAANGAMLANGKLLGTTALRPHLIWGPNDPHLVPRVLERAEAGRLKLVGSEDKLVDTI
YVGNAAYAHILAALTLSNGVSKGQVEDKNANQAACAGKAYYLSNDEPITMATMLNNILACKNLPPVTKRVPTNLAYAAGC
ILESIYALLGKKDEPIMTRFVARQLSTSHYFDISAAKRDLGYQALVSIDEGMEQLKASLQ

Sequences:

>Translated_380_residues
MSLVNTSQPPTQTPKLDDFLPQEQAALLALKQDVTRAFVTGAGGFLGKAICERLLAAGIEVIGFARGDYPALSAMGVEMV
KGDIADERALLGAMQGCDLVFHVASKAGVWGSKKSYFSPNVDGARHIIEACKACNIERLVYTSTPSVTFAGVDEVGNDES
APYAEQFLNYYGESKAIAEQMMLAANGAMLANGKLLGTTALRPHLIWGPNDPHLVPRVLERAEAGRLKLVGSEDKLVDTI
YVGNAAYAHILAALTLSNGVSKGQVEDKNANQAACAGKAYYLSNDEPITMATMLNNILACKNLPPVTKRVPTNLAYAAGC
ILESIYALLGKKDEPIMTRFVARQLSTSHYFDISAAKRDLGYQALVSIDEGMEQLKASLQ
>Mature_379_residues
SLVNTSQPPTQTPKLDDFLPQEQAALLALKQDVTRAFVTGAGGFLGKAICERLLAAGIEVIGFARGDYPALSAMGVEMVK
GDIADERALLGAMQGCDLVFHVASKAGVWGSKKSYFSPNVDGARHIIEACKACNIERLVYTSTPSVTFAGVDEVGNDESA
PYAEQFLNYYGESKAIAEQMMLAANGAMLANGKLLGTTALRPHLIWGPNDPHLVPRVLERAEAGRLKLVGSEDKLVDTIY
VGNAAYAHILAALTLSNGVSKGQVEDKNANQAACAGKAYYLSNDEPITMATMLNNILACKNLPPVTKRVPTNLAYAAGCI
LESIYALLGKKDEPIMTRFVARQLSTSHYFDISAAKRDLGYQALVSIDEGMEQLKASLQ

Specific function: 3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidation and isomerization of cholesterol, pregnenolone, and dehydroepiandrosterone (DHEA) into cholest-4-en-3-one, progesterone, and androsterone, respectively [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-beta-HSD family [H]

Homologues:

Organism=Homo sapiens, GI193211614, Length=367, Percent_Identity=34.0599455040872, Blast_Score=187, Evalue=2e-47,
Organism=Homo sapiens, GI8393516, Length=367, Percent_Identity=34.0599455040872, Blast_Score=187, Evalue=2e-47,
Organism=Homo sapiens, GI310132178, Length=357, Percent_Identity=31.3725490196078, Blast_Score=158, Evalue=8e-39,
Organism=Homo sapiens, GI310113012, Length=357, Percent_Identity=31.3725490196078, Blast_Score=158, Evalue=8e-39,
Organism=Homo sapiens, GI239745448, Length=357, Percent_Identity=31.3725490196078, Blast_Score=158, Evalue=9e-39,
Organism=Homo sapiens, GI116268111, Length=352, Percent_Identity=31.25, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI260763931, Length=358, Percent_Identity=31.0055865921788, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI4504509, Length=358, Percent_Identity=31.0055865921788, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI4504507, Length=358, Percent_Identity=31.2849162011173, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI19923621, Length=361, Percent_Identity=27.9778393351801, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI310113808, Length=263, Percent_Identity=28.1368821292776, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI310127990, Length=263, Percent_Identity=27.7566539923954, Blast_Score=76, Evalue=5e-14,
Organism=Escherichia coli, GI87081792, Length=360, Percent_Identity=26.6666666666667, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1786974, Length=294, Percent_Identity=26.530612244898, Blast_Score=62, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17570557, Length=342, Percent_Identity=23.9766081871345, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI193211272, Length=338, Percent_Identity=24.2603550295858, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17509709, Length=307, Percent_Identity=25.4071661237785, Blast_Score=76, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6321437, Length=363, Percent_Identity=29.4765840220386, Blast_Score=137, Evalue=4e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =1.1.1.145; =5.3.3.1 [H]

Molecular weight: Translated: 40677; Mature: 40546

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLVNTSQPPTQTPKLDDFLPQEQAALLALKQDVTRAFVTGAGGFLGKAICERLLAAGIE
CCCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCC
VIGFARGDYPALSAMGVEMVKGDIADERALLGAMQGCDLVFHVASKAGVWGSKKSYFSPN
EEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCC
VDGARHIIEACKACNIERLVYTSTPSVTFAGVDEVGNDESAPYAEQFLNYYGESKAIAEQ
CCHHHHHHHHHHHCCCCEEEEECCCCEEEECCHHCCCCCCCCHHHHHHHHHCCHHHHHHH
MMLAANGAMLANGKLLGTTALRPHLIWGPNDPHLVPRVLERAEAGRLKLVGSEDKLVDTI
HHHHCCCCEEECCEEEEECCCCCEEEECCCCCCHHHHHHHHHCCCCEEEECCCCCEEEEE
YVGNAAYAHILAALTLSNGVSKGQVEDKNANQAACAGKAYYLSNDEPITMATMLNNILAC
EECCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCEEEECCCCCEEHHHHHHHHHHH
KNLPPVTKRVPTNLAYAAGCILESIYALLGKKDEPIMTRFVARQLSTSHYFDISAAKRDL
CCCCCHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEHHHHHHHC
GYQALVSIDEGMEQLKASLQ
CHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SLVNTSQPPTQTPKLDDFLPQEQAALLALKQDVTRAFVTGAGGFLGKAICERLLAAGIE
CCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCC
VIGFARGDYPALSAMGVEMVKGDIADERALLGAMQGCDLVFHVASKAGVWGSKKSYFSPN
EEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCC
VDGARHIIEACKACNIERLVYTSTPSVTFAGVDEVGNDESAPYAEQFLNYYGESKAIAEQ
CCHHHHHHHHHHHCCCCEEEEECCCCEEEECCHHCCCCCCCCHHHHHHHHHCCHHHHHHH
MMLAANGAMLANGKLLGTTALRPHLIWGPNDPHLVPRVLERAEAGRLKLVGSEDKLVDTI
HHHHCCCCEEECCEEEEECCCCCEEEECCCCCCHHHHHHHHHCCCCEEEECCCCCEEEEE
YVGNAAYAHILAALTLSNGVSKGQVEDKNANQAACAGKAYYLSNDEPITMATMLNNILAC
EECCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCEEEECCCCCEEHHHHHHHHHHH
KNLPPVTKRVPTNLAYAAGCILESIYALLGKKDEPIMTRFVARQLSTSHYFDISAAKRDL
CCCCCHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEHHHHHHHC
GYQALVSIDEGMEQLKASLQ
CHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]