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The map label for this gene is msbB [H]

Identifier: 157962288

GI number: 157962288

Start: 3009622

End: 3010560

Strand: Reverse

Name: msbB [H]

Synonym: Spea_2467

Alternate gene names: 157962288

Gene position: 3010560-3009622 (Counterclockwise)

Preceding gene: 157962289

Following gene: 157962276

Centisome position: 58.18

GC content: 43.98

Gene sequence:

>939_bases
TTGTCTAGAAAGCCGAAGTATTTTGACCGACGTTTTAAGCTTAGTTTGTTGCACCCAAGATATTGGTTAACCTGGGTCGC
CATTCTAGTGCTGGTGGTATTTGGTCTTATGCCTGCAAGGTTAAGGGATCCAATCGCCCGTACACTGGCTAAATTAGTGA
TGAAAATTGCTAAGAAGCCAATTAAGGTTGCTAGGGCAAATTTGACCACTTGTTTCCCTGAAAAATCAGCCGATGAGATT
GAAGCGTTAGTTAAAGATAACGTCGAAAACTTTGTGATGATCTTGCTGGCACAATCAGAATTGATGTTAAAGTCTCGTGC
TCATATGAGACGTCGAGTGCAGCTGCATGGATTTGAGCATGTTGAGAATGCACGTGCAGCGGGTCAGCCAGTGATTTTTA
TCATGCCGCATGTATGGGGGATTGAATACGCAGGTTTAAGGCTGAACATCGATTTACCTATGGTTTCGATGGCTAAGGCT
CACAAGAATGATCTGTTTAACTGGTTTAATAACCGTATGCGTAGCTCCCAAGGCGGCAATATTTATATGCGTGAAGCAGG
GATCAGAGCCTTGCTTACCGAGCTTAAGCAAGATAACAGCTTCTTTTACCTACCCGATGAAGATCTTGGGCGAGATAAGA
GTATCTTTGCGCCCTTTTTAGGTACCACTAAGGCGACATTACCTGTTGTAGGCCGTTTAGCTCAGGCCGGTAACGCCCAA
GTTATGCCGGTCAAGATTGGCTATGATCAAGTTAAGCGCCATTTTGATTTAACTGTGATGCCTGCAATCTCCCCTGAAAC
CATGCAGGGTAAAGAAAATGAAGCCATTGCGTTGAATAAGGCGGTAGAGCAGGTGATCTTAGCTTACCCAGAGCAGTATA
TGTGGTTTTTAAAGGTATTAAAGACACGACCTGAAGGCGAAGAGTCTATCTATAAGTAG

Upstream 100 bases:

>100_bases
AAGAGGCTAACGCAAAGAAATAATTGCTTTAGCACTGTAATGAAGAGGCCACTCAATTGAGTGGCTTTTTCTGTTTCCAT
TTGCTGTAGAGGTTTTATCC

Downstream 100 bases:

>100_bases
TCTTTTCAATAAATTGTTGCAGCCGATAACTTGTCTGGTTTTGGGTTTTAGGGCAGTACATATTGATGTCCCGTCCTGAA
ATAGGTTGACGGTTTTTACT

Product: lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 312; Mature: 311

Protein sequence:

>312_residues
MSRKPKYFDRRFKLSLLHPRYWLTWVAILVLVVFGLMPARLRDPIARTLAKLVMKIAKKPIKVARANLTTCFPEKSADEI
EALVKDNVENFVMILLAQSELMLKSRAHMRRRVQLHGFEHVENARAAGQPVIFIMPHVWGIEYAGLRLNIDLPMVSMAKA
HKNDLFNWFNNRMRSSQGGNIYMREAGIRALLTELKQDNSFFYLPDEDLGRDKSIFAPFLGTTKATLPVVGRLAQAGNAQ
VMPVKIGYDQVKRHFDLTVMPAISPETMQGKENEAIALNKAVEQVILAYPEQYMWFLKVLKTRPEGEESIYK

Sequences:

>Translated_312_residues
MSRKPKYFDRRFKLSLLHPRYWLTWVAILVLVVFGLMPARLRDPIARTLAKLVMKIAKKPIKVARANLTTCFPEKSADEI
EALVKDNVENFVMILLAQSELMLKSRAHMRRRVQLHGFEHVENARAAGQPVIFIMPHVWGIEYAGLRLNIDLPMVSMAKA
HKNDLFNWFNNRMRSSQGGNIYMREAGIRALLTELKQDNSFFYLPDEDLGRDKSIFAPFLGTTKATLPVVGRLAQAGNAQ
VMPVKIGYDQVKRHFDLTVMPAISPETMQGKENEAIALNKAVEQVILAYPEQYMWFLKVLKTRPEGEESIYK
>Mature_311_residues
SRKPKYFDRRFKLSLLHPRYWLTWVAILVLVVFGLMPARLRDPIARTLAKLVMKIAKKPIKVARANLTTCFPEKSADEIE
ALVKDNVENFVMILLAQSELMLKSRAHMRRRVQLHGFEHVENARAAGQPVIFIMPHVWGIEYAGLRLNIDLPMVSMAKAH
KNDLFNWFNNRMRSSQGGNIYMREAGIRALLTELKQDNSFFYLPDEDLGRDKSIFAPFLGTTKATLPVVGRLAQAGNAQV
MPVKIGYDQVKRHFDLTVMPAISPETMQGKENEAIALNKAVEQVILAYPEQYMWFLKVLKTRPEGEESIYK

Specific function: Transfers myristate or laurate, activated on ACP, to (KDO)2-(lauroyl)-lipid IVA [H]

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family [H]

Homologues:

Organism=Escherichia coli, GI1788161, Length=302, Percent_Identity=33.112582781457, Blast_Score=178, Evalue=4e-46,
Organism=Escherichia coli, GI87082094, Length=310, Percent_Identity=30.3225806451613, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1787292, Length=304, Percent_Identity=28.2894736842105, Blast_Score=143, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004960
- InterPro:   IPR011921 [H]

Pfam domain/function: PF03279 Lip_A_acyltrans [H]

EC number: 2.3.1.-

Molecular weight: Translated: 35772; Mature: 35640

Theoretical pI: Translated: 10.36; Mature: 10.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRKPKYFDRRFKLSLLHPRYWLTWVAILVLVVFGLMPARLRDPIARTLAKLVMKIAKKP
CCCCCCHHHHHEEEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCC
IKVARANLTTCFPEKSADEIEALVKDNVENFVMILLAQSELMLKSRAHMRRRVQLHGFEH
HHHHHCCCCEECCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
VENARAAGQPVIFIMPHVWGIEYAGLRLNIDLPMVSMAKAHKNDLFNWFNNRMRSSQGGN
HHHHHHCCCCEEEEECCCCCCEECCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
IYMREAGIRALLTELKQDNSFFYLPDEDLGRDKSIFAPFLGTTKATLPVVGRLAQAGNAQ
EEEEHHHHHHHHHHHHCCCCEEECCCHHCCCCCCHHHHHHCCCHHHHHHHHHHHHCCCCE
VMPVKIGYDQVKRHFDLTVMPAISPETMQGKENEAIALNKAVEQVILAYPEQYMWFLKVL
EEEEECCHHHHHHHCCEEEECCCCCHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHH
KTRPEGEESIYK
HCCCCCHHHCCC
>Mature Secondary Structure 
SRKPKYFDRRFKLSLLHPRYWLTWVAILVLVVFGLMPARLRDPIARTLAKLVMKIAKKP
CCCCCHHHHHEEEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCC
IKVARANLTTCFPEKSADEIEALVKDNVENFVMILLAQSELMLKSRAHMRRRVQLHGFEH
HHHHHCCCCEECCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
VENARAAGQPVIFIMPHVWGIEYAGLRLNIDLPMVSMAKAHKNDLFNWFNNRMRSSQGGN
HHHHHHCCCCEEEEECCCCCCEECCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
IYMREAGIRALLTELKQDNSFFYLPDEDLGRDKSIFAPFLGTTKATLPVVGRLAQAGNAQ
EEEEHHHHHHHHHHHHCCCCEEECCCHHCCCCCCHHHHHHCCCHHHHHHHHHHHHCCCCE
VMPVKIGYDQVKRHFDLTVMPAISPETMQGKENEAIALNKAVEQVILAYPEQYMWFLKVL
EEEEECCHHHHHHHCCEEEECCCCCHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHH
KTRPEGEESIYK
HCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]